Cover Photograph: Upper left: Newt (P. waltl) juveniles and Xenopus froglets were subjected to agarose-embedded heat-shock (AeHS) method. GFP expression was induced specifically in the regenerating limbs (see Matsubara et al. 86–93). Upper right: Schematic diagram of the intermittent hypoxia exposure model. Pimonidazole-positive cells were detected in the pulmonary tissues of Xenopus laevis during repeated dives, indicating tissue hypoxia (see Fujiyama, 94–99). Lower left: The intercalary element of Zhangixalus schlegelii (arrowheads). Such skeletal elements are seen between the terminal and subterminal phalanges of all the digits of arboreal frogs (see Nakanishi, 100–108). Lower right: Tetraspanin9 expressions in Xenopus laevis (above) and X. tropicalis embryo (below) hybridized in the same tube. (see Kuriyama and Tanaka, 109–119). Bottom right corner: A 48 hpf early four-armed pluteus taken through a confocal microscope. Catecholamine-positive cells are colored in yellow (see Kalachev and Tankovich, 120–131).
A tidal pool dinoflagellate, Chiharadinium hexapraecingulum (T. Horiguchi & Chihara) Dawut & T. Horiguchi gen. & comb. nov. Upper row: Light micrographs of lateral view, ventral view, epifluorescence micrograph of motile cells and scanning electron micrograph of apical view of a cell. Lower row: Scanning electron micrographs of ventral view, left lateral view and antapical view of motile cells. Establishment of a new genus Chiharadinium is reported by Dawut et al. in this issue.
Cover Image An infl orescence of Clerodendrum izuinsulare (left) and C. trichotomum (right). Photos by Natsuki Aihara in Niijima Island (left) and by Takashi Miyake in Izu Peninsula (right), Japan. Miyake et al., doi.org/10.1111/1442-1984.12287
Jackson County, Illinois, U.S.A., Photo by Nathan Soley (right) Champaign County, Illinois, U.S.A., Photo by Nathan Soley (left) Soley and Sipes “Reproductive biology and pollinators of the invasive shrub Autumn olive (Elaeagnus umbellata Thunberg)”. https://doi.org/10.1111/1442-1984.12307
This work studied two-photon collagen crosslinking (CXL) in human corneal lenticules induced by near infrared femtosecond laser, possessing the advantages of multiphoton absorption and near-infrared light. The corneal stiffness can be enhanced by up to 296% without significantly reducing corneal transparency (<3%), paving the way to treat ophthalmic disorders such as keratoconus by 3D CXL of in vivo human cornea with higher safety, precision and efficacy. Further details can be found in the article by Zhenzhou Cheng, Nan Zhang, Le Chang, Pengfei Qi, Lin Zhang, Lie Lin, Yan Wang, and Weiwei Liu ( e202200160 ).
An optical fan was demonstrated to screen leukemia cells from the blood sample at the single‐cell level in a noninvasive and noncontact manner. Further details can be found in the article by Xiaoshuai Liu, Yuchao Li, Xiaohao Xu, Yao Zhang, Baojun Li ( e201900155 ).
Cover Image The pistillate-phase flowers of Artabotrys (Annonaceae): A. blumei (left) and A. brachypetalus (right). Photos by Junhao Chen, taken in Hong Kong and South Africa, respectively (DOI: 10.1111/1442-1984.12273 ).
Beautiful underwater view of an edible brown alga, Sargassum fusiforme (Fucales, Phaeophyceae) at the rocky shore in Sakurajima, Kagoshima, Japan (Photo by Ryuta Terada). Characteristic responses of the PSII photochemical efficiency on desiccation and salinity gradients in S. fusiforme are reported by Yonemori et al. in this issue.
A Orobanche boninsimae and its visitors, Japanese white-eyes (Zosterops japonicus). Photo by Akihiro Nishimura at Chichijima Island, the Bonin Islands, Japan. Nishimura & Takayama (2023) “First record of potential bird pollination in the holoparasitic genus Orobanche L.” Plant Species Biology, 6–17 https://doi.org/10.1111/1442-1984.12389
Cover Image Tulipa pumila. This photo was taken by Sandro Pratesi at Doccino at the municipality of Riparbella, province of Pisa, Tuscany, Italy (DOI: 10.1111/1442-1984.12267 ).
Leptocereus santamarinae. In Herradura beach in Las Tunas Province. Photo by Duniel Barrios. Flores et al. https://esj-journals.onlinelibrary.wiley.com/doi/abs/10.1111/1442-1984.12334 (right) Echinocereus enneacanthus (Cactaceae). Spring of 2020, Mapimi Biosphere Reserve, Southern Chihuahuan Desert, Durango, Mexico. Photo by José Antonio Aranda-Pineda. Aranda-Pineda et al. https://esj-journals.onlinelibrary.wiley.com/doi/abs/10.1111/1442-1984.12329 (left)
Left: Isodon shikokianus and Bombus diversus Right: Isodon shikokianus and Bombus honshuensis Both Photos by Ikumi Dohzono at Mt. Hyonosen, Hyogo Prefecture, Japan. Ogishima, M., Hoshino, Y., Horie, S., Yamashiro, T., Maki, M., Suzuki, K., & Dohzono, I. (2022). Secondary contact and adaptation to local pollinator assemblages mediate geographical variation in corolla length in Isodon shikokianus. Plant Species Biology, 222–230. https://doi.org/10.1111/1442-1984.12370
Lake Inle, a pristine lake in Myanmar, is known to harbor a number of endemic aquatic species and is a home to an enigmatic cyanobacterium Oscillatoria kawamurae, which was first reported in Lake Biwa (Japan). Macroscopic trichome (upper left), microscopic image (upper middle). Photos by Phong San (Lake Inle) and Nanda Kyaw Thu (O. kawamurae). See Thu et al. in this issue. Cover picture from: Article link here
The detection of colon cancer by using Poincaré sphere and 2D polarimetric imaging approach. Further details can be found in the article by Deyan Ivanov, Viktor Dremin, Alexander Bykov, Ekaterina Borisova, et al. ( e202000082 ).
CRISPR-Cas9 system with PEG-mediated transfection was efficient for genome editing in Ulva prolifera. U. prolifera (left) and male gametes (middle). Wild type (upper right) and the genome-edited strain (lower right) cultured in 2-FA selection medium, and each mutation site on the APT gene. See Ichihara et al. in this issue.
A high‐efficiency computer‐aided diagnostic model of ovarian cancer was developed, integrating SHG imaging technology for non‐invasive imaging of living tissue and machine learning method based on radiomics and TPOT. This model can rapidly, non‐destructively, and accurately perform ovarian cancer diagnosis and has great potential in improving diagnostic efficacy and efficiency of medical pathologists. Further details can be found in the article by Guangxing Wang, Yang Sun, Youting Chen, Qiqi Gao, Dongqing Peng, Hongxin Lin, Zhenlin Zhan, Zhiyi Liu, and Shuangmu Zhuo ( e202000050 ).
Peptoids belong to a class of sequence-controlled polymers containing N-alkylglycine units. Ren et al. use tandem mass spectrometry to characterize the fragmentation patterns of some singly and doubly protonated peptoids with one basic residue at different positions. Upon collision-induced dissociation, a charged peptoid dissociates into two sets of charged fragments: B-ions, containing the N-terminal residue (structure on the left); and Y-ions, containing the C-terminal residue (structure on the right). The Y-ions are of higher abundance than the B-ions, revealing that the C-terminal fragments have a higher affinity toward the charge carrier, the proton. (DOI: 10.1002/bip.23358 )
A novel 0.9 mm-diameter intravascular photoacoustic catheter with coaxial excitation and detection was developed to overcome the limitation of imaging range. A miniature ring-shaped ultrasound transducer with a 0.18 mm-diameter orifice in the center was successfully fabricated. The results demonstrated that the coaxial catheter exhibited much better photoacoustic/ultrasound imaging performance from the intima to the adventitia. Further details can be found in the article by Riqiang Lin, Qi Zhang, Shengmiao Lv, Jiaming Zhang, Xiatian Wang, Dongliang Shi, Xiaojing Gong, and Kwok-ho La ( e202200269 ).
A false‐colored and merged image of fresh, ex vivo rat kidney acquired using an excitation‐scanning hyperspectral imaging system. The spectral image was acquired using excitation wavelengths from 360 to 550 nm. Colors represent principal components extracted from a spectral image cube featuring no added labels or markers. Further details can be found in the article by Peter F. Favreau, Joshua A. Deal, Bradley Harris, et al. ( e201900183 ).
