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1.
Sulfate-reducing bacteria (SRB) in anoxic waters and sediments are the major producers of methylmercury in aquatic systems. Although a considerable amount of work has addressed the environmental factors that control methylmercury formation and the conditions that control bioavailability of inorganic mercury to SRB, little work has been undertaken analyzing the biochemical mechanism of methylmercury production. The acetyl-coenzyme A (CoA) pathway has been implicated as being key to mercury methylation in one SRB strain, Desulfovibrio desulfuricans LS, but this result has not been extended to other SRB species. To probe whether the acetyl-CoA pathway is the controlling biochemical process for methylmercury production in SRB, five incomplete-oxidizing SRB strains and two Desulfobacter strains that do not use the acetyl-CoA pathway for major carbon metabolism were assayed for methylmercury formation and acetyl-CoA pathway enzyme activities. Three of the SRB strains were also incubated with chloroform to inhibit the acetyl-CoA pathway. So far, all species that have been found to have acetyl-CoA activity are complete oxidizers that require the acetyl-CoA pathway for basic metabolism, as well as methylate mercury. Chloroform inhibits Hg methylation in these species either by blocking the methylating enzyme or by indirect effects on metabolism and growth. However, we have identified four incomplete-oxidizing strains that clearly do not utilize the acetyl-CoA pathway either for metabolism or mercury methylation (as confirmed by the absence of chloroform inhibition). Hg methylation is thus independent of the acetyl-CoA pathway and may not require vitamin B(12) in some and perhaps many incomplete-oxidizing SRB strains. 相似文献
2.
Javaherdashti R 《Applied microbiology and biotechnology》2011,91(6):1507-1517
Microbiologically Influenced Corrosion (MIC) is an electrochemical corrosion influenced by the presence/action of biological
agents such as, but not limited to, bacteria. One of the key elements of MIC is sulphate-reducing bacteria (SRB). There are
still many misunderstandings about these bacteria, their role in the deterioration of engineering materials and their importance
over other types of corrosion-related micro-/macro-organisms. SRB do not require oxygen, yet they can be found in oxygenated
environments; they are capable of tolerating a relative wide range of temperature, pH, chloride concentration and pressure
values. Not only can SRB have deteriorating impact on engineering materials, they are also capable of inducing harm to health
and agriculture. In this paper, after reviewing facts and figures regarding ecological and economical impacts of corrosion
in general and MIC, in particular, the central concept of MIC, that is, biofilm formation and its deterioration mechanisms
and the role of SRB in such mechanisms are described. Also, the possible enhancing role of SRB on stress corrosion cracking
of steels and the controversial concept of no relationship between the number of SRB and corrosion rate are addressed and
reviewed. 相似文献
3.
Mercury Methylation Independent of the Acetyl-Coenzyme A Pathway in Sulfate-Reducing Bacteria 总被引:1,自引:1,他引:0
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Sulfate-reducing bacteria (SRB) in anoxic waters and sediments are the major producers of methylmercury in aquatic systems. Although a considerable amount of work has addressed the environmental factors that control methylmercury formation and the conditions that control bioavailability of inorganic mercury to SRB, little work has been undertaken analyzing the biochemical mechanism of methylmercury production. The acetyl-coenzyme A (CoA) pathway has been implicated as being key to mercury methylation in one SRB strain, Desulfovibrio desulfuricans LS, but this result has not been extended to other SRB species. To probe whether the acetyl-CoA pathway is the controlling biochemical process for methylmercury production in SRB, five incomplete-oxidizing SRB strains and two Desulfobacter strains that do not use the acetyl-CoA pathway for major carbon metabolism were assayed for methylmercury formation and acetyl-CoA pathway enzyme activities. Three of the SRB strains were also incubated with chloroform to inhibit the acetyl-CoA pathway. So far, all species that have been found to have acetyl-CoA activity are complete oxidizers that require the acetyl-CoA pathway for basic metabolism, as well as methylate mercury. Chloroform inhibits Hg methylation in these species either by blocking the methylating enzyme or by indirect effects on metabolism and growth. However, we have identified four incomplete-oxidizing strains that clearly do not utilize the acetyl-CoA pathway either for metabolism or mercury methylation (as confirmed by the absence of chloroform inhibition). Hg methylation is thus independent of the acetyl-CoA pathway and may not require vitamin B12 in some and perhaps many incomplete-oxidizing SRB strains. 相似文献
4.
P. W. Blum A. E. Hershey M. T.-K. Tsui C. R. Hammerschmidt A. M. Agather 《Biogeochemistry》2018,137(1-2):181-195
Methylated mercury (MeHg) can be produced by all microbes possessing the genes hgcA and hgcB, which can include sulfate-reducing bacteria (SRB), iron-reducing bacteria (FeRB), methane-producing archaea (MPA), and other anaerobic microbes. These microbial groups compete for substrates, including hydrogen and acetate. When sulfate is in excess, SRB can outcompete other anaerobic microbes. However, low concentrations of sulfate, which often occur in stream sediments, are thought to reduce the relative importance of SRB. Although SRB are regarded as the primary contributors of MeHg in many aquatic environments, their significance may not be universal, and stream sediments are poorly studied with respect to microbial Hg methylation. We evaluated suppression of methanogenesis by SRB and the potential contributions from SRB, MPA and other MeHg producing microbes (including FeRB) to the production of MeHg in stream sediments from the North Carolina Piedmont region. Lower methanogenesis rates were observed when SRB were not inhibited, however, application of a sulfate-reduction inhibitor stimulated methanogenesis. Greater MeHg production occurred when SRB were active. Other MeHg producing microbes (i.e., FeRB) contributed significantly less MeHg production than SRB. MPA produced MeHg in negligible amounts. Our results suggest that SRB are responsible for the majority of MeHg production and suppress methanogenesis in mid-order stream sediments, similar to other freshwater sediments. Further investigation is needed to evaluate the generality of these findings to streams in other regions, and to determine the mechanisms regulating sulfate and electron acceptor availability and other potential factors governing Hg methylation and methane production in stream sediments. 相似文献
5.
脱氮硫杆菌生长特性及其对SRB生长的影响 总被引:10,自引:0,他引:10
由土壤中分离得到一株自养型的脱氮硫杆菌(Thiobacillus denitrigioans,硫杆菌属,硫杆菌科,革兰氏阴性化能自养细菌),该菌株的最佳生长pH为7.0。将此菌株与硫酸盐还原菌(Sulfate Reducing Bacteria,SRB,脱硫弧菌属,革兰氏阴性厌氧细菌)混合培养,测定SRB的菌量变化,结果表明,脱氮硫杆菌的生长抑制了硫酸盐还原菌的生长,降低了SRB的腐蚀性的代谢产物硫化物的浓度,腐蚀速率降低,有利于防治SRB引起的微生物腐蚀。 相似文献
6.
7.
Several biotreatmemt techniques for sulfate conversion by the sulfate reducing bacteria (SRB) have been proposed in the past, however few of them have been practically applied to treat sulfate containing acid mine drainage (AMD). This research deals with development of an innovative polypropylene hollow fiber membrane bioreactor system for the treatment of acid mine water from the Berkeley Pit, Butte, MT, using hydrogen consuming SRB biofilms. The advantages of using the membrane bioreactor over the conventional tall liquid phase sparged gas bioreactor systems are: large microporous membrane surface to the liquid phase; formation of hydrogen sulfide outside the membrane, preventing the mixing with the pressurized hydrogen gas inside the membrane; no requirement of gas recycle compressor; membrane surface is suitable for immobilization of active SRB, resulting in the formation of biofilms, thus preventing washout problems associated with suspended culture reactors; and lower operating costs in membrane bioreactors, eliminating gas recompression and gas recycle costs. Information is provided on sulfate reduction rate studies and on biokinetic tests with suspended SRB in anaerobic digester sludge and sediment master culture reactors and with SRB biofilms in bench-scale SRB membrane bioreactors. Biokinetic parameters have been determined using biokinetic models for the master culture and membrane bioreactor systems. Data are presented on the effect of acid mine water sulfate loading at 25, 50, 75 and 100 ml/min in scale-up SRB membrane units, under varied temperatures (25, 35 and 40 °C) to determine and optimize sulfate conversions for an effective AMD biotreatment. Pilot-scale studies have generated data on the effect of flow rates of acid mine water (MGD) and varied inlet sulfate concentrations in the influents on the resultant outlet sulfate concentration in the effluents and on the number of SRB membrane modules needed for the desired sulfate conversion in those systems. The pilot-scale data indicate that the SRB membrane bioreactors systems can be applied toward field-scale biotreatment of AMD and for recovery of high purity metals and an agriculturally usable water. 相似文献
8.
Mineral precipitation in microbial mats may have been the key to their preservation as fossil stromatolites, potentially documenting evidence of the earliest life on Earth. Two factors that contribute to carbonate mineral precipitation are the saturation index (SI) and the presence of nucleation sites. Both of these can be influenced by micro‐organisms, which can either alter SI through their metabolisms, or produce and consume organic substances such as extracellular polymeric substances (EPS) that can affect nucleation. It is the balance of individual metabolisms within the mat community that determines the pH and the dissolved inorganic carbon concentration, thereby potentially increasing the alkalinity and consequently the SI. Sulfate‐reducing bacteria (SRB) are an important component of this ‘alkalinity engine.’ The activity of SRB often peaks in layers where CaCO3 precipitates, and mineral precipitation has been demonstrated in SRB cultures; however, the effect of their metabolism on the alkalinity engine and actual contribution to mineral precipitation is the subject of controversy. Here, we show through culture experiments, theoretical calculations, and geochemical modeling studies that the pH, alkalinity, and organomineralization potential will vary depending on the type of electron donor. Specifically, hydrogen and formate can increase the pH, but electron donors like lactate and ethanol, and to a lesser extent glycolate, decrease the pH. The implication of this for the lithification of mats is that the combination of processes supplying electron donors and the utilization of these compounds by SRB may be critical to promoting mineral precipitation. 相似文献
9.
KEX2 mutations suppress RNA polymerase II mutants and alter the temperature range of yeast cell growth. 总被引:4,自引:2,他引:2
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Suppressors of a temperature-sensitive RNA polymerase II mutation were isolated to identify proteins that interact with RNA polymerase II in yeast cells. Ten independently isolated extragenic mutations that suppressed the temperature-sensitive mutation rpb1-1 and produced a cold-sensitive phenotype were all found to be alleles of a single gene, SRB1. An SRB1 partial deletion mutant was further investigated and found to exhibit several pleiotropic phenotypes. These included suppression of numerous temperature-sensitive RNA polymerase II mutations, alteration of the temperature growth range of cells containing wild-type RNA polymerase, and sterility of cells of alpha mating type. The ability of SRB1 mutations to suppress the temperature-sensitive phenotype of RNA polymerase II mutants did not extend to other temperature-sensitive mutants investigated. Isolation of the SRB1 gene revealed that SRB1 is KEX2. These results indicate that the KEX2 protease, whose only known substrates are hormone precursors, can have an important influence on RNA polymerase II and the temperature-dependent growth properties of yeast cells. 相似文献
10.
油田硫酸盐还原菌酸化腐蚀机制及防治研究进展 总被引:3,自引:0,他引:3
硫酸盐还原菌(Sulfate reducing bacteria,SRB)是一些厌氧产硫化氢的细菌的统称,是以有机物为养料的厌氧菌。它们广泛分布于pH值6-9的土壤、海水、河水、淤泥、地下管道、油气井、港湾及锈层中,它们生存于好气性硫细菌产生的沉积物下,其最适宜的生长温度是20-30℃,可以在高达50-60℃的温度下生存,与腐蚀相关的最主要的是脱硫脱硫弧菌(Desulfovibrio desulfuricans)。 它们是许多腐蚀问题的主因,例如油田系统金属管路的腐蚀等。在海上油田生产中,海水常被注入油井用于进行2次采油。富含硫酸盐的海水能加速油藏中SRB的生长,随之H2S大量产生,引起油田水的酸化,H2S具有毒性和腐蚀性,增加石油和天然气中的硫含量,并可能引起油田堵塞。SRB引起的腐蚀问题是拭待解决的最主要问题。国内外治理该问题的途径主要有物理杀灭、添加化学杀菌剂等方法,但是这些方法成本高,持续效果不显著。近几年来国外学者开始重点关注利用生物竞争排斥技术(Bio-competitive inhibition technology,BCX)控制硫酸盐还原菌的生长代谢的方法,该方法的原理为通过加入特定的药剂,激活油藏中的本源微生物或加入外源微生物,使其与SRB竞争营养源或产生代谢物抑制SRB的生长代谢,进而抑制H2S的产生。GMT-LATA的科学家对在厌氧油气储层和开采系统中硝酸盐还原菌的作用进行了最早的研究,认为该细菌可以抑制硫酸盐还原菌的代谢活动。随后BCX技术已经在国外部分油田得到了应用,国内还没有在海油生产中应用的报道,但是也有学者对该方法进行了研究。 相似文献
11.
Sulphate-reducing bacteria (SRB) play a vital role both the carbon and sulphur cycles and thus are extremely important components
of the global microbial community. However, it is clear that the ecology, the distribution and activity of different SRB groups
is poorly understood. Probing of rRNA suggests that different sediments have distinctly different patterns of SRB with complex
factors controlling the activity of these organisms. The linking of community structure and function using sediment slurry
microcosms suggests that certain groups of SRB, e.g., Desulfobacter and Desulfobulbus, can be linked to the use of specific substrates in situ. However, it is still unclear what environmental substrates are utilised by the majority of known SRBs. The work to date
has greatly enhanced our understanding of the ecology of these organisms and is beginning to suggest patterns in their distribution
and activity that may be relevant to understanding microbial ecology in general.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
12.
The importance of sulfate-reducing bacteria (SRB) in nature has been widely recognized for many years. However, little is known about the ecology of SRB. The problem has been detecting, classifying, and quantifying these organisms. There are many shortcomings in the use of culture media for this purpose. As an alternative, fluorescent antibody (FA) techniques were considered as a method for the detection and identification of SRB. Antisera were prepared against whole cells of different species of SRB and evaluated for detection and identification of these organisms. Surface antigens of SRB were species specific. In addition, culture conditions influenced the expression of surface antigens, causing the antisera to be extremely specific. These results were confirmed by the sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE) profiles of membrane proteins. On the basis of this specificity, the application of FA produced against culture collection strains would have limited application for detecting, identifying, and enumerating these organisms in nature. 相似文献
13.
14.
硫酸盐还原菌(sulfate-reducing bacteria,SRB)是肠道菌群的重要组成成员之一。SRB在其增殖与新陈代谢过程中会产生硫化氢。现有研究显示,SRB的过度繁殖与炎症性肠病(inflammatory bowel disease,IBD)、肠易激综合征(irritable bowel syndrome,IBS)、乳糜泻(celiac disease,CLD)和结直肠癌(colorectal cancer,CRC)等密切相关,但目前尚无相关文献对SRB在肠道疾病中扮演的角色、致病机理和肠道微生态等研究进行系统性的综述。SRB在肠道疾病中的分布特征及其致病机制值得进一步总结与探讨。本文收集过去10年来发表的关于SRB与肠道疾病的文献并进行详细分析与归纳,对SRB在宿主肠道内的分布与生理特征、SRB在不同肠道疾病的相关分布特点,以及SRB致病机制的研究进展等方面进行详细论述,以期增加本领域研究人员对SRB的重视。同时,本文对未来如何深化SRB在肠道相关疾病的研究方向进行探讨,以期为SRB相关肠道疾病的预防与治疗提供一定参考。 相似文献
15.
Nitrite reductase activity of sulphate-reducing bacteria prevents their inhibition by nitrate-reducing,sulphide-oxidizing bacteria 总被引:6,自引:0,他引:6
Sulphate-reducing bacteria (SRB) can be inhibited by nitrate-reducing, sulphide-oxidizing bacteria (NR-SOB), despite the fact that these two groups are interdependent in many anaerobic environments. Practical applications of this inhibition include the reduction of sulphide concentrations in oil fields by nitrate injection. The NR-SOB Thiomicrospira sp. strain CVO was found to oxidize up to 15 mM sulphide, considerably more than three other NR-SOB strains that were tested. Sulphide oxidation increased the environmental redox potential (Eh) from -400 to +100 mV and gave 0.6 nitrite per nitrate reduced. Within the genus Desulfovibrio, strains Lac3 and Lac6 were inhibited by strain CVO and nitrate for the duration of the experiment, whereas inhibition of strains Lac15 and D. vulgaris Hildenborough was transient. The latter had very high nitrite reductase (Nrf) activity. Southern blotting with D. vulgaris nrf genes as a probe indicated the absence of homologous nrf genes from strains Lac3 and Lac6 and their presence in strain Lac15. With respect to SRB from other genera, inhibition of the known nitrite reducer Desulfobulbus propionicus by strain CVO and nitrate was transient, whereas inhibition of Desulfobacterium autotrophicum and Desulfobacter postgatei was long-lasting. The results indicate that inhibition of SRB by NR-SOB is caused by nitrite production. Nrf-containing SRB can overcome this inhibition by further reducing nitrite to ammonia, preventing a stalling of the favourable metabolic interactions between these two bacterial groups. Nrf, which is widely distributed in SRB, can thus be regarded as a resistance factor that prevents the inhibition of dissimilatory sulphate reduction by nitrite. 相似文献
16.
Biogeochemical transformations occurring in the anoxic zones of stratified sedimentary microbial communities can profoundly influence the isotopic and organic signatures preserved in the fossil record. Accordingly, we have determined carbon isotope discrimination that is associated with both heterotrophic and lithotrophic growth of pure cultures of sulfate-reducing bacteria (SRB). For heterotrophic-growth experiments, substrate consumption was monitored to completion. Sealed vessels containing SRB cultures were harvested at different time intervals, and delta(13)C values were determined for gaseous CO(2), organic substrates, and products such as biomass. For three of the four SRB, carbon isotope effects between the substrates, acetate or lactate and CO(2), and the cell biomass were small, ranging from 0 to 2 per thousand. However, for Desulfotomaculum acetoxidans, the carbon incorporated into biomass was isotopically heavier than the available substrates by 8 to 9 per thousand. SRB grown lithoautotrophically consumed less than 3% of the available CO(2) and exhibited substantial discrimination (calculated as isotope fractionation factors [alpha]), as follows: for Desulfobacterium autotrophicum, alpha values ranged from 1.0100 to 1.0123; for Desulfobacter hydrogenophilus, the alpha value was 0.0138, and for Desulfotomaculum acetoxidans, the alpha value was 1.0310. Mixotrophic growth of Desulfovibrio desulfuricans on acetate and CO(2) resulted in biomass with a delta(13)C composition intermediate to that of the substrates. The extent of fractionation depended on which enzymatic pathways were used, the direction in which the pathways operated, and the growth rate, but fractionation was not dependent on the growth phase. To the extent that environmental conditions affect the availability of organic substrates (e.g., acetate) and reducing power (e.g., H(2)), ecological forces can also influence carbon isotope discrimination by SRB. 相似文献
17.
水稻土中硫酸盐还原微生物研究进展 总被引:2,自引:0,他引:2
硫是水稻必需的营养元素之一.硫酸盐还原是硫元素生物地球化学循环中的关键步骤,在稻田土壤表层和水稻根际都十分活跃.介导硫酸盐还原过程的硫酸盐还原菌(sulfate- reducing bacteria, SRB)是稻田土壤中重要的功能菌群.它们不仅是硫元素生物地球化学循环的重要参与者,也是土壤中有机污染物降解的主要力量之一,发挥着重要的生态和环境功能.综述了稻田土壤中微生物参与的硫酸盐还原过程、SRB的生物多样性以及目前研究稻田土壤SRB主要采用的分子生态学方法,如末端限制性片段长度多样性(T-RFLP)、变性梯度凝胶电泳(DGGE)、实时荧光定量PCR(real-time PCR)、荧光原位杂交(FISH),并对水稻土壤中SRB的分子生态学研究方向进行了展望. 相似文献
18.
Linked Redox Precipitation of Sulfur and Selenium under Anaerobic Conditions by Sulfate-Reducing Bacterial Biofilms 总被引:5,自引:1,他引:4
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A biofilm-forming strain of sulfate-reducing bacteria (SRB), isolated from a naturally occurring mixed biofilm and identified by 16S rDNA analysis as a strain of Desulfomicrobium norvegicum, rapidly removed 200 μM selenite from solution during growth on lactate and sulfate. Elemental selenium and elemental sulfur were precipitated outside SRB cells. Precipitation occurred by an abiotic reaction with bacterially generated sulfide. This appears to be a generalized ability among SRB, arising from dissimilatory sulfide biogenesis, and can take place under low redox conditions and in the dark. The reaction represents a new means for the deposition of elemental sulfur by SRB under such conditions. A combination of transmission electron microscopy, environmental scanning electron microscopy, and cryostage field emission scanning electron microscopy were used to reveal the hydrated nature of SRB biofilms and to investigate the location of deposited sulfur-selenium in relation to biofilm elements. When pregrown SRB biofilms were exposed to a selenite-containing medium, nanometer-sized selenium-sulfur granules were precipitated within the biofilm matrix. Selenite was therefore shown to pass through the biofilm matrix before reacting with bacterially generated sulfide. This constitutes an efficient method for the removal of toxic concentrations of selenite from solution. Implications for environmental cycling and the fate of sulfur and selenium are discussed, and a general model for the potential action of SRB in selenium transformations is presented. 相似文献
19.
Dar SA Yao L van Dongen U Kuenen JG Muyzer G 《Applied and environmental microbiology》2007,73(2):594-604
Here we describe the diversity and activity of sulfate-reducing bacteria (SRB) in sulfidogenic bioreactors by using the simultaneous analysis of PCR products obtained from DNA and RNA of the 16S rRNA and dissimilatory sulfite reductase (dsrAB) genes. We subsequently analyzed the amplified gene fragments by using denaturing gradient gel electrophoresis (DGGE). We observed fewer bands in the RNA-based DGGE profiles than in the DNA-based profiles, indicating marked differences in the populations present and in those that were metabolically active at the time of sampling. Comparative sequence analyses of the bands obtained from rRNA and dsrB DGGE profiles were congruent, revealing the same SRB populations. Bioreactors that received either ethanol or isopropanol as an energy source showed the presence of SRB affiliated with Desulfobulbus rhabdoformis and/or Desulfovibrio sulfodismutans, as well as SRB related to the acetate-oxidizing Desulfobacca acetoxidans. The reactor that received wastewater containing a diverse mixture of organic compounds showed the presence of nutritionally versatile SRB affiliated with Desulfosarcina variabilis and another acetate-oxidizing SRB, affiliated with Desulfoarculus baarsii. In addition to DGGE analysis, we performed whole-cell hybridization with fluorescently labeled oligonucleotide probes to estimate the relative abundances of the dominant sulfate-reducing bacterial populations. Desulfobacca acetoxidans-like populations were most dominant (50 to 60%) relative to the total SRB communities, followed by Desulfovibrio-like populations (30 to 40%), and Desulfobulbus-like populations (15 to 20%). This study is the first to identify metabolically active SRB in sulfidogenic bioreactors by using the functional gene dsrAB as a molecular marker. The same approach can also be used to infer the ecological role of coexisting SRB in other habitats. 相似文献
20.
Linked redox precipitation of sulfur and selenium under anaerobic conditions by sulfate-reducing bacterial biofilms 总被引:5,自引:0,他引:5
A biofilm-forming strain of sulfate-reducing bacteria (SRB), isolated from a naturally occurring mixed biofilm and identified by 16S rDNA analysis as a strain of Desulfomicrobium norvegicum, rapidly removed 200 micro M selenite from solution during growth on lactate and sulfate. Elemental selenium and elemental sulfur were precipitated outside SRB cells. Precipitation occurred by an abiotic reaction with bacterially generated sulfide. This appears to be a generalized ability among SRB, arising from dissimilatory sulfide biogenesis, and can take place under low redox conditions and in the dark. The reaction represents a new means for the deposition of elemental sulfur by SRB under such conditions. A combination of transmission electron microscopy, environmental scanning electron microscopy, and cryostage field emission scanning electron microscopy were used to reveal the hydrated nature of SRB biofilms and to investigate the location of deposited sulfur-selenium in relation to biofilm elements. When pregrown SRB biofilms were exposed to a selenite-containing medium, nanometer-sized selenium-sulfur granules were precipitated within the biofilm matrix. Selenite was therefore shown to pass through the biofilm matrix before reacting with bacterially generated sulfide. This constitutes an efficient method for the removal of toxic concentrations of selenite from solution. Implications for environmental cycling and the fate of sulfur and selenium are discussed, and a general model for the potential action of SRB in selenium transformations is presented. 相似文献