Effect of elevated CO2 concentration on seedling growth rate and photosynthesis inHevea brasiliensis

To study the effect of elevated CO₂ concentration on plant growth and photosynthesis, two clones ofHevea brasiliensis were grown in polybags and exposed to elevated concentration (700±25ppm) for 60 days. There was higher biomass accumulation, leaf area and better growth when compared to ambient air grown plantso From A/Ci curves it is clear that photosynthetic rates increases with increase in CO₂ concentrations. After 60 days of exposure to higher CO₂ concentration, a decrease in the carbon assimilation rate was noticed.     

Photosynthetic characteristics of Cymbidium plantlet in vitro

The photosynthetic characteristics of the Cymbidium plantlet in vitro cultured on Hyponex-agar medium with 2% sucrose were determined based on the measurements of CO₂ concentration inside and outside of the culture vessels. The CO₂ measurements were made with a gas chromatograph at a PPF (photosynthetic photon flux) of 35, 102 and 226 mol m^-2 s^-1, a chamber air temperature of 15, 25 and 35°C and a CO₂ concentration outside the vessel of approximately 350, 1100 and 3000 ppm. The net photosynthetic rates were determined on individual plantlets and were expressed on a dry weight basis. The steady-state CO₂ concentration during the photoperiod was lower inside the vessel than outside the vessel at any PPF greater than 35 mol m^-2s^-1 and at any chamber air temperature. The photosynthetic response curves relating the net photosynthetic rate, PPF, and CO₂ concentration in the vessel and chamber air temperature were similar to those for Cymbidium plants grown outside and other C₃ plants grown outside under shade. The results indicate that CO₂ enrichment for the plantlets in vitro at a relatively high PPF would promote photosynthesis and hence the growth of chlorophyllous shoots/plantlets in vitro and that the plantlets in vitro would make photoautotrophic growth under environmental conditions favorable for photosynthesis.Abbreviations C_in CO₂ concentration in the culture vessel - C_out CO₂ concentration outside the vessel (in the culture room) - PPF photosynthetic photon flux     

High photosynthetic photon flux and high CO2 concentration under increased number of air exchanges promote growth and photosynthesis of four kinds of orchid plantlets in vitro

Summary In vitro plantlets of Phalaenopsis ‘Happy Valentine’, Neofinetia falcate Hu, Cymbidium kanran Makino, and Cymbidium goeringii Reichb. f. were grown under photoautotrophic [high photosynthetic photon flux (PPF), high CO₂ concentration, and increased number of air exchanges] and heterotrophic (low PPF, low CO₂ concentration, no air exchanges) culture conditions. After 40 d of culture, a significant difference in plantlet growth was observed between the two cultures. Total fresh and dry mass were on average 1.5 times greater in photoautotrophic culture than in heterotrophic culture. Higher net photosynthetic rates were also observed for Phalaenopsis in photoautotrophic culture. In photoautotrophic culture, little difference was observed in air temperature between the inside and outside of the culture vessel, whereas in heterotrophic culture, air temperature inside the culture vessel was 1–2°C higher than that outside the culture vessel. Relative humidity inside the culture vessel was remarkably different between the two cultures: 83–85% in photoautotrophic culture and 97–99% in heterotrophic culture. These results indicated that growth and net photosynthetic rate of in vitro orchid plantlets were susceptible to the culture environments such as PPF, CO₂ concentration, relative humidity (RH), and the number of air exchanges, which would allow a more efficient micropropagation system for these orchid plants.     

The effect of light quality and gibberellic acid (GA3) on photosynthesis and respiration rates of pea seedlings

CO₂ exchange were measured on pea seedlings (Pisum sativum L. var. Bördi) cultivated from seeds imbibed either in water (C-plants) or in gibberellic acid (GA₃) at the concentration of 25 g/1 (GA-plants), and then grown under 17 W/m² blue light (B-plants) or 11 W/m² red light (R-plants).When measured under the same light conditions as during growth the net photosynthesis (APS) rate in B-plants was about twice higher than that in R-plants. Dark respiration (DR) rate was 70% higher in B- than in R-plants. Red light retarded the development of photosynthetic activity, but GA₃ suppressed this effect. The hormone enhanced net photosynthesis and dark respiration to the same extent.When measured under saturating white light net photosynthesis rate of C-plants was also two times higher in B-plants than in R-plants. Growth conditions had only a slight effect on the APS of GA-plants under white light. APS rates of GA-plants grown under red light were higher under white light than those of C-plants, but lower than those of plants grown under blue light.We assume that blue light induced formation of plants that were adapted to higher light intensity: red light had an opposite effect, whereas gibberellic acid induced formation of plants that were adapted to medium light intensity.     

Ecosystem carbon fluxes of a ryegrass and clover fodder crop in a Mediterranean environment

A mixture of ryegrass (Lolium italicum A. Braun) and clover (Trifolium alexandrinum L.) was sown in Eboli (Salerno, Southern Italy) in September 2007. Crop growth, leaf and canopy gas exchange and ecophysiological traits were monitored throughout the growth cycle. The gross primary production (GPP) was not affected by air temperature (T _air); on the contrary the ecosystem respiration (R _eco) decreased as T _air decreased while net ecosystem CO₂ exchange (NEE) increased. When was normalized with leaf area index (LAI), GPP decreased with T _air, a likely response to cold that down-regulated canopy photosynthesis in order to optimize the light use at low winter temperatures. Net photosynthetic rates (P _N), the effective quantum yield of PSII (Φ_PSII) and photosynthetic pigment content were higher in clover than ryegrass, in relation to the higher leaf N content. The lower Φ_PSII in ryegrass was linked to lower photochemical quenching coefficient (q_P) values, due to a reduced number of reaction centres, in agreement with the lowest Chl a content. This behaviour can be considered as an adaptive strategy to cold to avoid photooxidative damage at low temperature rather than an impairment of PSII complexes.     

Factors influencing the capacity for photosynthetic carbon assimilation in barley leaves at low temperatures

The aim of this work was to examine the effect upon photosynthetic capacity of short-term exposure (up to 10 h) to low temperatures (5° C) of darkened leaves of barley (Hordeum vulgare L.) plants. The carbohydrate content, metabolite status and the photosynthetic rate of leaves were measured at low temperature, high light and higher than ambient CO₂. Under these conditions we could detect whether previous exposure of leaves to low temperature overcame the limitation by phosphate which occurs in leaves of plants not previously exposed to low temperatures. The rates of CO₂ assimilation measured at 8° C differed by as much as twofold, depending upon the pretreatment. (i) Leaves from plants which had previously been darkened for 24 h had a low content of carbohydrate, had the lowest CO₂-assimilation rates at low temperature, and photosynthesis was limited by carbohydrate, as shown by a large stimulation of photosynthesis by feeding glucose, (ii) Leaves from plants which had previously been illuminated for 24 h and which contained large carbohydrate reserves showed an accumulation of phosphorylated intermediates and higher CO₂-assimilation rates at low temperature, but nevertheless remained limited by phosphate, (iii) Maximum rates of CO₂ assimilation at low temperature were observed in leaves which had intermediate reserves of carbohydrate or in leaves which were rich in carbohydrate and which were also fed phosphate. It is suggested that carbohydrate reserves potentiate the system for the achievement of high rates of photosynthesis at low temperatures by accumulation of photosynthetic intermediates such as hexose phosphates, but that this potential cannot be realised if, at the same time, carbohydrate accumulation is itself leading to feedback inhibition of photosynthesis. This work was supported by the Agricultural and Food Research Council, UK (Research grant PG50/67) and by the Science and Engineering Reserach Council, UK. C.A.L. was supported by the British Council, by an Overseas Research Student Award and by the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil.     

Control Mechanisms of Photosynthetic Capacity Under Elevated CO2 Concentration: Evidence from Three Experiments with Norway Spruce Trees

Twelve-year-old Norway spruce (Picea abies [L.] Karst.) were exposed to ambient (AC) or elevated (EC) [ambient + 350 mol(CO₂) mol^–1] CO₂ concentration [CO₂] using the facilities of open-top-chambers (OTCs) and glass domes (GDs). A combination of gas exchange measurements and application of a biochemical model of photosynthesis were used for the evaluation of CO₂ assimilation characteristics. Morphological change was assessed on the base of specific leaf area (SLA). Nitrogen (N) content in the assimilation apparatus was considered a main factor influencing the biochemical capacity. Three experiments confirm the hypothesis that an adjustment of photosynthetic capacity under EC is controlled by the combination of biochemical, morphological, and physiological feedback mechanisms. We observed periodicity of down-regulation of photosynthetic capacity (Experiment No. 1) during the vegetation seasons. In the spring months (May–June), i.e. during the occurrence of active carbon sink associated with the formation of new foliage, up-regulation (10–35 %) of photosynthetic capacity (P _Nsat) was observed. On the contrary, in the autumn months (September–October) down-regulation (25–35 %) of P _Nsat was recorded that was mainly associated with reduced carbon sink strength and biochemical change, i.e. decrease of N status (up to 32 %) and accumulation of saccharides (up to 72 %) in leaves. Different adjustments of photosynthetic activities were observed in current (C) and one-year-old (C-1) needles exposed to EC (Experiment No. 2). Strong down-regulation of P _Nsat and the diminution of the initial stimulation of photosynthetic rate (P _Nmax) was associated with decreases of both ribulose-1,5-bisphosphate carboxylase/oxygenase carboxylation activity (by 32 %) and RuBP regeneration (by 40 %). This performance was tightly correlated with the absence of active carbon sinks, decrease of N content, and starch accumulation in C-1 needles. Finally, different responses of sun- and shade-adapted needles to EC (Experiment No. 3) were associated with the balance between morphological and biochemical changes. Observed P _Nsat down-regulation (by 22 %) of exposed needles in EC was predominantly caused by effects of both higher assimilate accumulation and stronger N dilution, resulting from higher absolute photosynthetic rates and incident irradiances in the upper canopy.     

Performance of two Picea abies (L.) Karst. stands at different stages of decline

Summary Photosynthetic rates and nutrient contents of spruce needles were measured in a region with high levels of air pollution in NE Bavaria, Germany (FRG), and compared to spruce grown under clean air conditions at Craigieburn, in the South Island of New Zealand (NZ). The absolute rates of CO₂ uptake, the slope of the CO₂ response curve at 240 l l^–1 internal CO₂ concentration, and the change of photosynthetic rates with needle age at ambient and saturated CO₂ concentrations were virtually identical at both measuring sites. These results confirm an earlier conclusion, that there is no long-term effect of atmospheric pollutants directly on photosynthetic CO₂ uptake rates with persistent exposure at the FRG site to high levels of anthropogenic air pollution. Photosynthetic capacity at saturating CO₂ concentration was three times higher in the NZ spruce. Needles with high photosynthetic capacity in NZ had lower nitrogen and higher calcium concentrations per unit dry weight but higher concentrations of nitrogen, phosphorus, potassium, magnesium and calcium per unit leaf area, and twice the specific leaf weight.     

The effect of water flow on photosynthetic processes of the alga Ulva lactuca L.

Photosynthetic rates of aquatic macrophytes are affected by the diffusion of carbon through the boundary layer which decreases with increasing flow velocities. This is shown by a significant increase in photosynthetic rates of Ulva lactuca fronds exposed to friction velocities (u _*) between 0.0 and 0.3 cm s^–1. No further increase in photosynthetic rates is observed at u _* values between 0.3 and 1.0 cm s^–1. Stagnating flow conditions at saturating light levels caused a 29% reduction in photosynthetic rates. Experiments in a closed system indicate that Ulva may utilize internal carbon sources during periods of stagnation. On a subtidal rock directly exposed to wave action, Ulva lactuca is not exposed to such conditions while in a wave flushed intertidal pool stagnation occurs during very short time intervals based on in situ measurements of u _* levels on fronds of this species using a microprocessor-controlled, autonomous recorder and hot-film sensors.     

Nitrogen assimilation and transpiration: key processes conditioning responsiveness of wheat to elevated [CO2] and temperature

Although climate scenarios have predicted an increase in [CO₂] and temperature conditions, to date few experiments have focused on the interaction of [CO₂] and temperature effects in wheat development. Recent evidence suggests that photosynthetic acclimation is linked to the photorespiration and N assimilation inhibition of plants exposed to elevated CO₂. The main goal of this study was to analyze the effect of interacting [CO₂] and temperature on leaf photorespiration, C/N metabolism and N transport in wheat plants exposed to elevated [CO₂] and temperature conditions. For this purpose, wheat plants were exposed to elevated [CO₂] (400 vs 700 µmol mol^?1) and temperature (ambient vs ambient + 4°C) in CO₂ gradient greenhouses during the entire life cycle. Although at the agronomic level, elevated temperature had no effect on plant biomass, physiological analyses revealed that combined elevated [CO₂] and temperature negatively affected photosynthetic performance. The limited energy levels resulting from the reduced respiratory and photorespiration rates of such plants were apparently inadequate to sustain nitrate reductase activity. Inhibited N assimilation was associated with a strong reduction in amino acid content, conditioned leaf soluble protein content and constrained leaf N status. Therefore, the plant response to elevated [CO₂] and elevated temperature resulted in photosynthetic acclimation. The reduction in transpiration rates induced limitations in nutrient transport in leaves of plants exposed to elevated [CO₂] and temperature, led to mineral depletion and therefore contributed to the inhibition of photosynthetic activity.     

Effects of an Experimental Increase of Temperature and Drought on the Photosynthetic Performance of Two Ericaceous Shrub Species Along a North–South European Gradient

Plant ecophysiological changes in response to climatic change may be different in northern and southern European countries because different abiotic factors constrain plant physiological activity. We studied the effects of experimental warming and drought on the photosynthetic performance of two ericaceous shrubs (Erica multiflora and Calluna vulgaris) along a European gradient of temperature and precipitation (UK, Denmark, The Netherlands, and Spain). At each site, a passive warming treatment was applied during the night throughout the whole year, whereas the drought treatment excluded rain events over 6–10 weeks during the growing season. We measured leaf gas exchange, chlorophyll a fluorescence, and leaf carbon isotope ratio (¹³C) during the growing seasons of 1999 and 2000. Leaf net photosynthetic rates clearly followed a gradient from northern to southern countries in agreement with the geographical gradient in water availability. Accordingly, there was a strong correlation between net photosynthetic rates and the accumulated rainfall over the growing season. Droughted plants showed lower leaf gas exchange rates than control plants in the four sites. Interestingly, although leaf photosynthetic rates decreased along the precipitation gradient and in response to drought treatment, droughted plants were able to maintain higher leaf photosynthetic rates than control plants in relation to the accumulated rainfall over the months previous to the measurements. Droughted plants also showed higher values of potential photochemical efficiency (F _v/F _m) in relation to controls, mainly at midday. The warming treatment did not affect significantly any of the studied instantaneous ecophysiological variables..     

Seasonal changes in net photosynthesis rates and photosynthetic capacity in leaves of Cistus salvifolius,a European mediterranean semi-deciduous shrub

Summary During five different periods between Nov. 1982 and Aug. 1983, the diurnal patterns exhibited in photosynthetic CO₂ uptake and stomatal conductance were observed under natural conditions on twigs of Cistus salvifolius, a Mediterranean semi-deciduous shrub which retains a significant proportion of its leaves through the summer drought. During the same periods, net photosynthesis at saturating CO₂ partial pressure was measured on the same twigs as a function of irradiance at different temperatures. From these data, photosynthetic capacity, defined here as the CO₂- and light-saturated net photosynthesis rate, was obtained as a function of leaf temperature. C. salvifolius is a winter growing species, shoot growth being initiated in Nov. and continuing through May. Photosynthetic capacity was quite high in Nov., March and June, exceeding 40 mol m^-2 s^-1 at optimum temperature. In Dec., photosynthetic capacity was somewhat reduced, perhaps due to low night-time temperatures (<5°C) during the measurement period. In Aug., capacity in oversummering shoots at optimum temperature fell to less than 8 mol m^-2 s^-1, due to water trees and perhaps leaf aging. Seasonal changes in maximal photosynthetic rates under ambient conditions were similar, and like those found in co-occurring evergreen sclerophylls. Like the evergreens, Cistus demonstrated considerable stomatal control of transpirational water loss, particularly in oversummering leaves. During each measurement period except Aug. when capacity was quite low, the maximum rates of net photosynthesis measured under ambient conditions were less than half the measured photosynthetic capacities at comparable temperatures, suggesting an apparent excess nitrogen investment in the photosynthetic apparatus.     

COMPARATIVE PHOTOSYNTHESIS OF TWO SPECIES OF INTERTIDAL EPIPHYTIC MACROALGAE ON MANGROVE ROOTS DURING SUBMERSION AND EMERSION

Photosynthesis and dark respiration rates were measured in water and in air, and the capacity to recover photosynthetic activity from emersion stress was examined for two species of intertidal, epiphytic macroalgae—Bostrychia calliptera (Montagne) Montagne and Caloglossa leprieurii (Montagne) J. Agardh—collected on prop roots of the red mangrove Rhizophora mangle L. in Buenaventura Bay, Pacific coast of Colombia. In both species, net photosynthetic rates were significantly higher under submersed conditions. Maximum photosynthetic rates (P_max) in water and in air were highest in B. calliptera, 126 ± 4 versus 52 ± 9 μmol O₂·mg chl a⁻¹·h⁻¹, respectively. In C. leprieurii, P_max of submerged plants in water and in air were 98 ± 9 versus 30 ± 11 μmol O₂·mg chla⁻¹·h⁻¹. The photoinhibition model of Platt et al. (1980) was used to fit the experimental data in both water and air for both species. Photoinhibition occurred at irradiance as low as 200 μmol·m⁻²·s⁻¹. The photosynthesis–light response curves demonstrated an adaptation to shaded habitats for both species, as light compensation points in water and air for both species were below 17 ± 5 μmol·m⁻²·s⁻¹. The rate of dehydration was significantly lower in thalli of B. calliptera compared to C. leprieurii. An increase of photosynthetic activity in B. calliptera was evident between 5% and 15% water loss, but rates decreased thereafter with declining water content. In C. leprieurii, desiccation negatively influenced photosynthetic rates that significantly decreased linearly with declining water content. In B. calliptera, net photosynthesis reached zero only at a water content between 29% and 35%, whereas in C. leprieurii no net photosynthesis occurred in plants containing less than about 50% of their relative water content. Resubmerged plants ofB. calliptera exhibited 100% photosynthetic recovery after 45 min, whereas C. leprieurii recovered 100% at about 120 min. On the basis of the comparison of rates of light-saturated net photosynthesis for B. calliptera in air versus in water, aerial photosynthetic activity ranged from 35% to 42% of that in water, whereas the emersed photosynthetic capacity of C. leprieurii ranged from 24% to 29% of that in water. Using tidal predictions and the emersed photosynthetic rates, a carbon balance model was constructed for both species over a single daylight period. The calculations indicated that emersed photosynthesis increased average daily carbon production of B. calliptera by 17% and C. leprieuri by 12%. The physiological responses to desiccation stress and the photosynthetic recovery capacities between species correlated with, and may determine, their vertical distribution in the mangrove habitats of Buenaventura Bay.     

Inhibition of photosynthesis ofPopulus euramericana andHelianthus annuus by SO2, NO2 and O3

Populus euramericana cv. I-214 andHelianthus annuus L. cv. Russian Mammoth were exposed to various concentrations of O₃ SO₂ or NO₂ for 2 h in a cylindrical assimilation chamber. The threshold concentrations of air pollutants for inhibition of net photosynthesis differed between the two species and also between the pollutants tested. Furthermore, the lethal concentrations where the net photosynthetic rates were completely inhibited, also differed between species and between pollutants. For SO₂ and NO₂,P. euramericana was more tolerant photosynthetically thanH. annuus when related to the concentration of pollutants used during the experiment. However, when related to the cumulative uptake rate of each pollutant, the photosynthetic tolerance of the two species was similar. In contrast to the effects of SO₂ or NO₂, the influence of O₃ on net photosynthesis was quite different. The relative rates of net photosynthesis in both species showed the same linear relationship with O₃ concentration. However, the relationship between the relative rate of net photosynthesis and the cumulative uptake rate of O₃ differed between the two species, although it was linear in both cases.     

Submerged versus air-exposed intertidal macrophyte productivity: from physiological to community-level assessments

The photosynthetic productivity of the intertidal communities dominated by the seagrass Zostera noltii and the cordgrass Spartina maritima was assessed in two contrasting situations during a tidal cycle, i.e., air exposure and water immersion. Two complementary methods were used: infra red gas analysis of CO₂ flux measurements in whole communities and chlorophyll a fluorescence measurements of individual plants photosynthetic activity. Higher photosynthetic rates of Z. noltii in air were observed both at the individual plants response level determined by chlorophyll fluorescence and at the community level measured as gas exchange (CO₂ uptake). S. maritima plants consistently showed low photosynthetic response when immersed. Gross community production (GCP) measured as carbon dioxide uptake was always higher in air than in water for both communities. When immersed, the GCP of both communities was similar. However, when exposed to the air, the GCP of the S. maritima community was higher than the one of Z. noltii's. The key factor in CO₂ assimilation by air-exposed Z. noltii was the retention of water in sediment microdepressions. During low tide, depressions in the sediment retain a considerable amount of water, enough to maintain leaf hydration. In these conditions, rapid air-water CO₂ diffusion occurs, making it readily available to plants. The community gas exchange measurements compared well with the fluorescence indications. Both Z. noltii and S. maritima were shown to be responsible for the overall pattern of photosynthetic carbon fixation within their respective communities, both during submersion and emersion periods. The short-term incubations method described in this report proved to be a valuable tool for field measurements of intertidal lagoon productivity. It provides fast and precise values of carbon dioxide fixation, both in submerged and air-exposed communities.     

Environmental effects on circadian rhythms in photosynthesis and stomatal opening

Persistent circadian rhythms in photosynthesis and stomatal opening occurred in bean (Phaseolus vulgaris L.) plants transferred from a natural photoperiod to a variety of constant conditions. Photosynthesis, measured as carbon assimilation, and stomatal opening, as conductance to water vapor, oscillated with a freerunning period close to 24 h under constant moderate light, as well as under light-limiting and CO₂-limiting conditions. The rhythms damped under constant conditions conducive to high photosynthetic rates, as did rates of carbon assimilation and stomatal conductance, and this damping correlated with the accumulation of carbohydrate. No rhythm in respiration occurred in plants transferred to constant darkness, and the rhythm in stomatal opening damped rapidly in constant darkness. Damping of rhythms also occurred in leaflets exposed to constant light and CO₂-free air, demonstrating that active photosynthesis and not simply light was necessary for sustained expression of these rhythms. This is CIWDPB Publication No. 1142 This research was supported by National Science Foundation grant BSR 8717422 (C.B.F.) and a U.S. Department of Agriculture training grant to Stanford University (T.L.H.).     

Overcoming drought-induced decreases in soybean leaf photosynthesis by measuring with CO2-enriched air

Soybean [Glycine max (L.) Merr. cv. Williams 82 and A3127] plants were grown in the field under long-term soil moisture deficit and irrigation to determine the effects of severe drought stress on the photosynthetic capacity of soybean leaves. Afternoon leaf water potentials, stomatal conductances, intercellular CO₂ concentrations and CO₂-assimilation rates for the two soil moisture treatments were compared during the pod elongation and seed enlargement stages of crop development. Leaf CO₂-assimilation rates were measured with either ambient (340 l CO₂ l^–1) or CO₂-enriched (1800 l CO₂ l^–1) air. Although seed yield and leaf area per plant were decreased an average of 48 and 31%, respectively, as a result of drought stress, leaf water potentials were reduced only an average of 0.27 MPa during the sampling period. Afternoon leaf CO₂-assimilation rates measured with ambient air were decreased an average of 56 and 49% by soil moisture deficit for Williams 82 and A3127, respectively. The reductions in leaf photosynthesis of both cultivars were associated with similar decreases in leaf stomatal conductance and with small increases in leaf intercellular CO₂ concentration. When the CO₂-enriched air was used, similar afternoon leaf CO₂-assimilation rates were found between the soil moisture treatments at each stage of crop development. These results suggest that photosynthetic capacity of soybean leaves is not reduced by severe soil moisture deficit when a stress develops gradually under field conditions.Abbreviations C_i intercellular CO₂ concentrations - A_a rates of CO₂ assimilation measured with ambient air - A_e rates of CO₂ assimilation measured with CO₂-enriched air - g_s stomatal conductances - RuBPCase ribulose-1,5-bisphosphate carboxylase     

Water loss from cut grass with special reference to hay-making

Water loss from cut grass was studied to determine factors limiting the drying process. An apparatus, used to measure water loss at 28±1°C from blotting paper and from leaves and stem internodes of cocksfoot, consisted of four channels in which air speed was controlled at 25–80 cm s^-1 and relative humidity at 7–68%. The maximum rate of water loss from wet blotting paper was 10500 mg water dm^-2 h^-1 but from leaves and stem internodes supplied with water it was less than 250 mg dm^-2 h^-1. The rate of loss from both plant specimens and blotting paper was linearly related to the vapour pressure differences between the specimen and the surrounding air but was not increased when air speed was changed from 40 to 80 cm s^-1. Grass specimens supplied with water had lower rates of water loss than wet blotting paper because of tissue resistances which were calculated for (a) untreated leaf and stem specimens, (b) rubbed leaves, (c) cut leaves, (d) leaves exposed to steam for 60 s. Treatments (b)-(d) greatly reduced tissue resistances. The rates of drying of leaves and stem internodes not supplied with water changed only slightly in response to faster air speeds but were significantly increased by treatments (b), (c), (c_s) (split stems), (d) and (e) (exposure to petroleum vapour for 60 s). The most effective treatments trebled the drying rates of leaves and increased the drying rates of stem internodes by 10 times. Reductions in relative humidity had little effect on drying rate following treatments (a), (b), (c) and (d), but when treatments (c_s) and (e) were given, additional significant increases in drying rates were obtained when the relative humidity was reduced. Grass specimens given the most effective treatments and dried under the most favourable conditions did not utilize the full drying capacity of the environment, for the rates of water loss from these specimens were at least three times lower than those from wet blotting paper. The results indicate that high rates of drying could be achieved at 28 ^oC or similar temperatures if practical treatments were developed to remove or greatly reduce the high resistance to water loss in cut grass.     

Effect of varying environments on photosynthetic parameters of C3, C3-C4 and C4 species of Panicum

Summary CO₂ exchange characteristics and the activity of the carboxylating enzymes phosphoenolpyruvate carboxylase (PEP-C, E.C. 4.1.1.31) and ribulose 1,5-bisphosphate carboxylase (RuBP-C, E.C. 4.1.1.39) during one year in the greenhouse and at two levels of light and temperature in growth chambers were determined in the C₃-C₄ intermediate species P. milioides Nees ex. Trin. These results were compared with those of P. bisulcatum Thumb. (C₃) and P. maximum Jacq. (C₄). Under all tested conditions, and even when the influence of leaf surface temperature on photosynthetic rates and CO₂ compensation points were measured, the biochemical and physiological behaviour of the C₃-C₄ intermediate was more similar to that of the C₃ plant than the C₄ species. The C₄ plant P. maximum, however, responded positively, mainly in terms of PEP-C activity and photosynthetic rate, to the regime of high light and temperature. The results presented indicate that in the C₃-C₄ Panicum grown in high light and temperature no direct relationships between a low CO₂ compesation point and superior growth are evident. It has still to be clarified why in nature a photosynthetic-photorespiratory pathway leading to an intermediate CO₂ compensation value has evolved in P. milioides.     

Photosynthetic characteristics of leaves developed at different irradiances and temperatures: an extension of the current hypothesis

Photosynthetic characteristics at high measurement irradiance were analyzed for single leaves of two C₃ and one C₄ species grown under twenty one combinations of irradiance level, irradiance duration, and air temperature in order to test the idea that photosynthetic characteristies developed by leaves in different environments are controlled by the daily amount of photosynthesis. Photosynthetic rates per unit area and mesophyll conductances at 25°C and air levels of CO₂ and O₂, and parameters for two photosynthesis models were used to characterize the photosynthetic properties of the leaves. Leaves with highest values of the photosynthetic parameters for each species were often developed in environments with irradiance levels below saturation for photosynthesis, and with only 12 hours of irradiance per day. Lower air temperature during growth increased the photosynthetic characteristics for a given irradiance regime. Photosynthetic characteristics had higher correlation coefficients with daily photosynthesis of mature leaves divided by 24-hour leaf elongation rates of young leaves, than with daily photosynthesis alone, indicating that photosynthetic characteristics may be related to a balance between photosynthesis and leaf expansion.