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Guangda Feng Songzhen Yang Yonghong Wang Qing Yao Honghui Zhu 《Current microbiology》2014,69(6):888-893
A Gram-negative bacterial strain, designated WB1T, was isolated from a domestic refrigerator in Guangzhou, PR China. Cells of strain WB1T were oxidase-negative, catalase-positive, strictly aerobic, non-spore-forming and non-motile coccobacilli with peritrichous fimbriae-like structures. The strain was able to grow at 10–40 °C with optimum growth at 28–30 °C, pH 6.0–8.0 (optimum, pH 7.0) and 0–6 % NaCl (w/v, optimum, 0.5 %). Phylogenetic analyses based on 16S rRNA gene and rpoB gene sequences revealed that strain WB1T belonged to the genus Acinetobacter and was most closely related to A. indicus DSM 25388T (97.2 % 16S rRNA gene sequence similarity) and A. radioresistens NBRC 102413T (96.8 %). The DNA G + C content of strain WB1T was 46.74 ± 0.04 mol % and the major fatty acids comprised summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C18:1 ω9c, C16:0 and C12:0. The predominant respiratory quinone was identified as Q-9 and the polar lipids as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and an unidentified phospholipid. Phenotypic, phylogenetic and chemotaxonomic data, including low DNA–DNA relatedness with closely related type strains, supported that strain WB1T represents a distinct novel species in the genus Acinetobacter, for which the name Acinetobacter refrigeratorensis sp. nov. was proposed. The type strain is WB1T (=GIMCC 1.663T = CCTCC AB 2014197T = KCTC 42011T). 相似文献
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Oh Byeong Seob Kim Ji-Sun Yu Seung Yeob Ryu Seoung Woo Park Seung-Hwan Kang Se Won Park Jam-Eon Choi Seung-Hyeon Han Kook-Il Lee Keun Chul Eom Mi Kyung Suh Min Kuk Kim Han Sol Lee Dong Ho Yoon Hyuk Kim Byung-Yong Lee Je Hee Lee Jung-Sook Lee Ju Huck 《Journal of microbiology (Seoul, Korea)》2020,58(2):99-104
Journal of Microbiology - An obligately anaerobic, Gram-stain-negative, non-motile, non-spore-forming, and coccobacilli-shaped bacterial strain, designated KGMB03119T, was isolated from human... 相似文献
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Helicobacter anseris sp. nov. and Helicobacter brantae sp. nov., Isolated from Feces of Resident Canada Geese in the Greater Boston Area
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James G. Fox Nancy S. Taylor Shelly Howe Michele Tidd Shilu Xu Bruce J. Paster Floyd E. Dewhirst 《Applied microbiology》2006,72(7):4633-4637
Numbers of nonmigratory Canada geese have increased substantially in the past decade, and they have become a nuisance in some urban areas. Because of their close contact with humans in parks and areas adjacent to surface waterways, contact with their feces poses a zoonotic risk. A total of 97 geese from 10 separate geographic locales in the greater Boston area had their feces sampled for detection of Helicobacter spp. Identification of Helicobacter spp. based on 16S rRNA genus-specific helicobacter primers was noted in 39 of 97 (40.2%) DNA fecal extracts. Twenty-seven (27.8%) of these geese had helicobacters isolated from their feces. A urease-positive novel species, Helicobacter anseris, based on phenotypic, biochemical, and 16S rRNA analyses, was isolated from 20 geese from seven different flocks. A second, novel, urease-negative Helicobacter sp., H. brantae, was identified in seven geese. Four geese had both novel Helicobacter spp. cultured from their feces. Whether these two novel helicobacters pose a zoonotic risk, similar to other enteric helicobacters (e.g., H. canadensis, previously isolated from diarrheic and bacteremic humans and from geese in Europe), will require further studies. 相似文献
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Strain IAM 14839, isolated from activated sludge in Japan, forms a visible floc and grows in the flocculated state. This bacterium is Gram-negative, rod-shaped, strictly aerobic and highly motile with a single polar flagellum. Both oxidase and catalase activities are positive. No growth was observed on sugars. The strain can grow at 20 degrees C, but does not grow at 37 degrees C. The G+C content of DNA is 66.3 mol% and Q-8 is the major quinone. The major cellular fatty acids are 16:1omega7c, 16:0, 18:1omega7c, 2OH 16:0, 3OH 10:0. The 16S rDNA sequence analysis indicated that the bacterium clustered within the genus Comamonas. On the basis of the phylogenetic analysis and phenotypic properties, it is proposed that the strain IAM 14839T be classified in a novel species of the genus Comamonas, Comamonas badia sp. nov. The type strain is IAM 14839T (=KCTC 12244T ). 相似文献
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A Gram-positive, catalase and oxidase positive, rod-shaped bacteria, and spore-forming, designated as J20-3T was isolated from a peat soil, collected near a coal mine at Prokopyevsk, (GPS; N53°52′51″, E86°43′39″) Kemerovo Oblast, Russia. A polyphasic taxonomy study using phenotypic, phylogenetic, and genotypic method was performed to characterize strain J20-3T. Comparative 16S rRNA gene sequence analysis indicated that strain J20-3T represented a novel subline within the genus Cohnella in the family Paenibacillaceae. According to 16S rRNA gene sequence, strain J20-3T showed 93.7–97.2 % similarity levels with other Cohnella species. Strain J20-3T exhibited relatively low level of DNA–DNA hybridization value with type strains KACC 11643T (40 %), KACC 11771T (37.5 %), and KACC 15372T (30.5 %). The strain showed typical chemotaxonomic characteristic of the genus Cohnella, with the presence of predominant respiratory quinone MK-7; major fatty acids are C15:0, C16:0, iso, and C16:0. The DNA G+C content of the strain J20-3T was 56.3 mol%. The polar lipid profile of the strain J20-3T included major amount of diphosphatidylglycerol, phosphatidylglycerol, and phosphoatidylethanolamine. On the basis of its phenotypic and genotypic properties, and its phylogenetic distinctiveness, strain J20-3T should be classified as a novel species in the genus Cohnella, for which the name Cohnella humi sp. nov. is proposed. 相似文献
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Yan-yu Meng Yun-bin Fu Meng-yu Wang Yong Zhao Qi-shun Liu Yu-guang Du 《Current microbiology》2013,66(5):487-492
A Gram-negative, aerobic, rod-shaped, motile, non-spore-forming bacterial strain, designated 13-QT, was isolated from seaside soil under the stacks of the red algae in Hainan province in China. Identification was carried out on the basis of polyphasic taxonomy. Phylogenetic analysis of 16S rRNA gene sequences showed that strain 13-QT belonged to the genus Pedobacter, and the highest similarity was 94.4 % with Pedobacter terricola KCTC 12876T. Strain 13-QT was able to grow at 10–40 °C, in pH 5.0–10.0, in the presence of 0–2.0 % NaCl. The major fatty acids were iso-C15:0 (40.4 %), summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1 ω7c) (18.9 %) and iso-C17:0 3-OH (18.4 %). The predominant menaquinone was MK-7. The G+C content of the genomic DNA was 42.7 mol%. Strain 13-QT could be distinguished from the nearest phylogenetic neighbors by various chemotaxonomic and phenotypic properties. The results of the polyphasic analyses suggested that strain 13-QT should be considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter hainanensis sp. nov. is proposed. The type strain is 13-QT (=CCTCC AB 2012076T = NRRL B-59850T). 相似文献
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Sathiyaraj Srinivasan Jae-Jin Lee Sang-Seob Lee Myung Kyum Kim 《Current microbiology》2014,69(3):263-269
A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated as SWU8T, was isolated from a mountain soil collected in Seoul Women’s University campus at South Korea. Phylogenic analysis, using 16S rRNA gene sequence of the new isolate, showed that strain SWU8T belongs to the genus Pontibacter. The highest sequence similarities were 96.2 % with Pontibacter saemangeumensis GCM0142T, 95.5 % with Pontibacter toksunensis ZLD-7T, 95.3 % with Pontibacter roseus DSM 17521T, and 95.1 % with Pontibacter odishensis JC130T. Chemotaxonomic data showed that the most abundant fatty acids were summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B; 26.9 %), iso-C15:0 (25.6 %), and iso-C17:0 3OH (10.6 %), and major polar lipid was phosphatidylethanolamine. The DNA G+C content of strain SWU8T was 48.5 mol%. Together, the phenotypic, phylogenetic, and chemotaxonomic data supported that strain SWU8T presents a novel species of the genus Pontibacter, for which the name Pontibacter humi sp. nov. is proposed. The type strain is SWU8T (=KEMC 9004-131T = JCM 19178T). 相似文献
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The strain designated as AB21T was isolated from chloroethylenes contaminated soil. Cells are gram-negative, aerobic, non-spore-forming, and motile rods. Phylogenetic analysis based on 16S rRNA gene sequence showed that it belonged to the genus Rhizobium, and was closely related to Rhizobium sullae IS 123T (97.4 %), Rhizobium yanglingense SH 22623T (97.2 %), Rhizobium gallicum R 602spT (97.1 %), Rhizobium alamii GBV 016T (97.0 %), and Rhizobium monogolense USDA 1844T (97.0 %). It showed less than 97 % identity with the remaining Rhizobium species. This novel isolate grew optimally at 25–37 °C (optimum, 30 °C) and pH 6–9 (optimum, pH 8.0). It grew in the presence of 0–4 % (w/v) NaCl, tolerating a 4 % (w/v) NaCl. DNA–DNA hybridization experiment shows less than 53 % binding with closely related Rhizobium. Predominant quinone is ubiquinone (Q-10). The major fatty acids were summed feature 8 (composed of C18:1 ω7c/C18:1 ω6c), C19:0 cyclo ω8c, and C16:0. The G+C molar content is 62.5 mol%. Based on the polyphasic analysis, strain AB21T is referred to be a novel species of the genus Rhizobium for which the name Rhizobium halotolerans sp. nov. is proposed. The type strain is AB21T (=KEMC 224-056T = JCM 17536T). 相似文献
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Kunwar Digvijay Narayan Shachindra K. Pandey Subrata K. Das 《Current microbiology》2010,60(4):248-253
Comamonas thiooxidans (strain S23T) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA–DNA homology
study showed 55% similarity with Comamonas testosteroni KCTC2990T and 52% with Comamonas composti LMG24008T, the nearest phylogenetic relative (16S rRNA sequence similarity <97%). Comparative genomic fingerprinting by using ERIC
and Rep-PCR further delineated species identity of the strain S23T for which Comamonas thiooxidans sp. nov. is proposed. In addition, thiosulfate oxidation potential of the strain S23T was compared with Comamonas testosteroni and Comamonas composti. 相似文献
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A Gram-negative, motile, rod-shaped, non-spore-forming bacterial strain, designated as Ko03(T), was isolated from microbial granules, and was characterized, using a polyphasic approach, in order to determine its taxonomic position. The isolate was positive for catalase and oxidase, but negative for gelatinase and beta-galactosidase. Phylogenetic analyses using the 16S rRNA gene sequence showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus Comamonas, its closest neighbors being Comamonas koreensis KCTC 12005(T) (95.9% sequence similarity), Comamonas nitrativorans DSM 13191(T) (95.7%), and Comamonas odontotermitis LMG 23579(T) (95.7%). Strain Ko03(T) had a genomic DNA G+C content of 68.4 mol% and the predominant respiratory quinone was Q-8. The major fatty acids were C(16:1) omega7c (44.7%), C(16:0) (28.1%), C(18:1) (16.1%), and C(10:0) 3-OH (3.5%). These chemo-taxonomic results supported the affiliation of strain Ko03(T) to the genus Comamonas. However, low 16S rRNA gene sequence similarity values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain Ko03(T) from recognized Comamonas species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Ko03(T) represents a novel species of the genus Comamonas, for which the name Comamonas granuli sp. nov. is proposed. The type strain is Ko03(T) (= KCTC 12199(T) = NBRC 101663(T)). 相似文献
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