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1.
Shuhui Li Kai Tang Keshao Liu Chang-Ping Yu Nianzhi Jiao 《Antonie van Leeuwenhoek》2014,105(1):245-251
A Gram-negative, aerobic, motile by single polar flagellum, short rod-shaped bacterium was isolated from a deep-seawater sample of the Southeastern Pacific Ocean. Growth was found to occur at 10–40 °C, at pH 4.0–10.0 and in the presence of 0–9.0 % (w/v) NaCl. The phylogenetic tree based on 16S rRNA gene sequences showed that strain JLT2013T fell within a clade comprising species of the genus Parvularcula and formed a coherent cluster with Parvularcula lutaonensis CC-MMS-1T (neighbour-joining phylogenetic tree) or Parvularcula dongshanensis SH25T (maximum-likelihood and maximum-parsimony phylogenetic trees). Sequence similarity analyses based on 16S rRNA gene sequences revealed that strain JLT2013T shows high sequence similarity to P. lutaonensis CC-MMS-1T (96.7 %), P. dongshanensis SH25T (96.0 %) and Parvularcula bermudensis HTCC2503T (95.2 %). The major cellular fatty acids were identified as C12:0 (34.3 %), summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) (10.9 %), C16:0 (10.0 %) and C17:1 ω6c (7.2 %). The polar lipids were found to include diphosphatidylglycerol, phosphatidylglycerol, three sphingoglycolipids and three unknown glycolipids. Strain JLT2013T was found to contain Q-10 as the predominant quinone. The DNA G+C content was determined to be 66.3 mol%. In the light of the phenotypic characteristics, chemotaxonomic features and phylogenetic evidence gathered in this study, strain JLT2013T (=LMG 27362T = CGMCC 1.12400T) should be classified as a novel species in the genus Parvularcula, for which the name Parvularcula oceanus sp. nov. is proposed. 相似文献
2.
Yanxia Li Yuki Chan Yingnan Fu Rui Zhang Jill M. Y. Chiu 《Antonie van Leeuwenhoek》2013,104(6):983-991
A novel bacterial strain, JLT2006T, was isolated from the scleractinian coral Platygyra carnosus, located in Hong Kong, China. Cells of this strain were Gram-negative, rod-shaped or oval-shaped and motile by the means of polar flagella. They formed faint-yellow, round colonies on marine agar medium. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that the strain JLT2006T belonged to the class Gammaproteobacteria and was most closely related to Alteromonas-like bacteria of the genera Psychromonas, Pseudoalteromonas, Moritella, Shewanella and Ferrimonas, with less than 93 % sequence similarity. The predominant fatty acids were identified as C18:1ω7c/C18:1ω6c (23.0 %), C16:1ω7c/C16:1ω6c (18.2 %) and C16:0 (16.4 %). The quinone was menaquinone-7 (100 %). The polar lipids were determined to be phosphatidylglycerol, phosphatidylethanolamine, phospholipid, glycolipid and lipid. The genomic DNA G+C content was 40.3 mol%. Based on the 16S rRNA gene sequence as well as the physiological and biochemical features that separate the strain JLT2006T from other recognized bacteria, a novel species of a new genus with the name Coralslurrinella hongkonensis gen. nov., sp. nov. is proposed. The type strain is JLT2006T (=JCM 18796T = CGMCC 1.10992T). 相似文献
3.
A novel Gram-negative, orange-pigmented bacterial strain JLT2008T was isolated from the surface seawater of the Western Pacific and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JLT2008T belonged to the genus Erythrobacter, sharing the highest similarity (96.6 %) with Erythrobacter gangjinensis K7-2T and the lowest similarity (94.9 %) with Erythrobacter litoralis DSM 8509T. Strain JLT2008T did not contain bacteriochlorophyll a, and the predominant respiratory lipoquinone was ubiquinone-10. The major fatty acids were C18:1 ω7c, C16:0, C16:1 ω7c/C16:1 ω6c. The prominent polar lipids were sphingoglycolipid, phosphatidylethanolamine, and phosphatidylglycerol. The genomic G + C content was 60.1 mol %. Based on the polyphasic taxonomic data, a novel species within the genus Erythrobacter, and with the name Erythrobacter westpacificensis sp. nov., is proposed. The type strain is JLT2008T (=CGMCC 1.10993T = JCM 18014T). 相似文献
4.
Qipei Li Yongle Xu Keshao Liu Lanlan Cai Yingnan Fu Jia Sun Rui Zhang 《Current microbiology》2013,66(5):450-455
A Gram-negative, rod-shaped, non-spore-forming aerobic bacterium, motile with a single polar flagellum, strain JLT2005T, was isolated from surface seawater collected from the East China Sea and formed ivory white colonies on a rich organic medium. The strain was positive for catalase, oxidase, and urease. It grew in the presence of 0–12 % (w/v) NaCl (optimum 5 %), at 20–35 °C (optimum 25 °C), or at pH 6–10 (optimum pH 9). The major fatty acids (>10 %) were C18:1ω7c, C19:0ω8c cyclo, C16:0, and C18:0. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and five unidentified glycolipids. Ubiquinone-10 and Ubiquinone-11 were present as the major quinones. The DNA G+C content was 74.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain JLT2005T belongs to the genus Pelagibacterium in the family Hyphomicrobiaceae, class Alphaproteobacteria. The closest neighbors were Pelagibacterium halotolerans B2T (98.7 % similarity) and Pelagibacterium luteolum 1_C16_27T (97.1 % similarity). DNA–DNA relatedness values of strain JLT2005T with P. halotolerans B2T and with P. luteolum 1_C16_27T were 31.6 and 25 %. Evidence from genotypic, chemotaxonomic, and phenotypic data shows that strain JLT2005T represents a novel species of the genus Pelagibacterium, for which the name Pelagibacterium nitratireducens sp. nov is proposed. The type strain is JLT2005T (=CGMCC 1.10829T =JCM 17767T). 相似文献
5.
Guizhen Li Qiliang Lai Xiupian Liu Fengqin Sun Yaping Du Guangyu Li Zongze Shao 《Antonie van Leeuwenhoek》2013,104(6):1073-1081
A taxonomic study was carried out on strain 22II-S10r2T, which was isolated from the deep sea sediment of the Atlantic Ocean using oil-degrading enrichment. The bacterium was Gram-negative, oxidase positive and catalase negative, spherical in shape, and motile by polar flagella. Growth was observed at salinities of 0.5–7 % and at temperatures of 10–41 °C. The isolate was capable of aesculin hydrolysis, but unable to reduce nitrate to nitrite or degrade Tween 80 or gelatine. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S10r2T belonged to the family Ectothiorhodospiraceae, with highest sequence similarity to Thioalkalivibrio sulfidiphilus HL-EbGR7T (90.9 % similarity). The principal fatty acids were Sum In Feature 8 (C18:1 ω7c/ω6c (29.9 %), C18:1 ω9c (13.5 %), C16:1 ω5c (12.3 %), C12:03OH (6.8 %), C18:1 ω5c (5.7 %) and C16:0 (5.3 %). The G+C content of the chromosomal DNA was 60.7 mol%. The respiratory quinone was determined to be Q-7 (25 %) and Q-8 (75 %). Phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid, glycolipid, three phospholipids and lipid were present. The strain was aerobic, non-phototrophic and non-chemolithoautotrophic. The combined genotypic and phenotypic data show that strain 22II-S10r2T represents a novel species within a novel genus, for which the name Maricoccus atlantica gen. nov. sp. nov. is proposed, with the type strain 22II-S10r2T (=CGMCC NO.1.12317T = LMG 27155T = MCCC 1A09384T). 相似文献
6.
Olga I. Nedashkovskaya Anna M. Stenkova Natalia V. Zhukova Stefanie Van Trappen Jung-Sook Lee Seung Bum Kim 《Antonie van Leeuwenhoek》2013,103(1):69-77
A novel Gram-negative, facultatively anaerobic and motile bacterial strain, designated KMM 6351T, was isolated from the sea urchin Strongylocentrotus intermedius and examined using a polyphasic taxonomic approach. A phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strain formed a distinct phyletic line in the class Gammaproteobacteria and was most closely related to the genera Aliivibrio, Photobacterium and Vibrio. Strain KMM 6351T grows at 4–40 °C and with 0.5–12 % NaCl and decomposes aesculin, agar, gelatin, starch, chitin and DNA. The DNA G+C content of the strain was determined to be 46.1 mol%. The prevalent fatty acids were found to be C16:0, C18:1 ω7c, C12:0 3-OH and summed feature 3 (comprising C16:1 ω7c and/or iso-C15:0 2-OH fatty acids). The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The predominant ubiquinone was found to be Q-8. The results of the phenotypic, chemotaxonomic and genotypic analyses clearly indicated that the novel strain should be assigned to a new genus and species within the class γ-Proteobacteria for which the name Echinimonas agarilytica gen. nov., sp. nov. is proposed. The type strain is KMM 6351T (=KCTC 22996T = LMG 25420T). 相似文献
7.
Yingfang Lin Kai Tang Shuhui Li Keshao Liu Jia Sun Nianzhi Jiao 《Antonie van Leeuwenhoek》2014,106(3):507-513
A Gram-stain negative, oval-shaped, aerobic, catalase and oxidase-positive bacterium, designated JLT2014T, was isolated from a deep-seawater sample (obtained at a 2,000 m depth) of the Southeastern Pacific Ocean. The dominant fatty acids were identified as C18:1ω7c/C18:1ω6c, C16:0 and C10:0 3-OH, which altogether represented 60.1 % of the total. The predominant respiratory quinone was identified as Q-10. The G+C content of genomic DNA was determined to be 66.4 mol %. The major polar lipids were identified as phosphatidylethanolamine and diphosphatidylglycerol. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate can be affiliated with the Roseobacter clade within the family Rhodobacteraceae. Strain JLT2014T exhibited highest 16S rRNA gene sequence similarity value to Pelagibaca bermudensis HTCC2601T (sequence similarity value: 97.6 %). The DNA–DNA relatedness value between strain JLT2014T and P. bermudensis HTCC2601T was 46.9 ± 2 %. Based on phenotypic properties and phylogenetic analysis, the name Pelagibaca abyssi sp. nov. is proposed, with JLT2014T(=LMG 27363T=CGMCC 1.12376T) as the type strain. 相似文献
8.
Nupur Bhumika Vaidya Naga Radha Srinivas Tanuku Anil Kumar Pinnaka 《Antonie van Leeuwenhoek》2013,103(2):347-355
A novel marine, Gram-negative, rod-shaped bacterium, designated strain N9T, was isolated from a water sample of the sea shore at Visakhapatnam, Andhra Pradesh (India). Strain N9T was found to be positive for oxidase and catalase activities. The fatty acids were found to be dominated by C16:0, C18:1 ω7c and summed in feature 3 (C16:1 ω7c and/or C16:1 ω6c). Strain N9T was determined to contain Q-10 as the major respiratory quinone and phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, two phospholipids and four unidentified lipids as polar lipids. The DNA G+C content of the strain N9T was found to be 63 mol%. 16S rRNA gene sequence analysis indicated that Rhodobacter sphaeroides, Rhodobacter johrii, Pseudorhodobacter ferrugineus, Rhodobacter azotoformans, Rhodobacter ovatus and Pseudorhodobacter aquimaris were the nearest phylogenetic neighbours, with pair-wise sequence similarities of 95.43, 95.36, 94.24, 95.31, 95.60 and 94.74 %, respectively. Phylogenetic analysis showed that strain N9T formed a distinct branch within the family Rhodobacteraceae and clustered with the clade comprising species of the genus Pseudorhodobacter, together with species of the genera Roseicitreum, Roseinatronobacter, Roseibaca and Rhodobaca. Species of the genus Pseudorhodobacter are phylogenetically close with a 16S rRNA gene sequence dissimilarity of 5.9–7.3 % (92.7–94.1 % similarity). Based on the above-mentioned phenotypic characteristics and on phylogenetic inference, strain N9T is proposed as a representative of a new genus and a novel species of the family Rhodobacteraceae as Albirhodobacter marinus gen. nov., sp. nov. The type strain of Albirhodobacter marinus is N9 (= MTCC 11277T = JCM 17680T). 相似文献
9.
A Gram-negative, aerobic, non-motile, dark brown-coloured and rod-shaped bacterial strain, designated G-MB1T, was isolated from a tidal flat sediment of the South Sea, South Korea. Strain G-MB1T was found to grow optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain G-MB1T fell within the clade comprising Thalassomonas species, clustering with the type strains of Thalassomonas agarivorans, Thalassomonas loyana, Thalassomonas ganghwensis and Thalassomonas agariperforans, with which it exhibited 16S rRNA gene sequence similarity values of 96.0–96.9 %. The 16S rRNA gene sequence similarity values between strain G-MB1T and the type strains of the other Thalassomonas species were 94.6–95.1 %. Strain G-MB1T was found to contain Q-8 as the predominant ubiquinone and C16:0, C17:1 ω8c, C16:1 ω9c, C12:0 3-OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) as the major fatty acids. The major polar lipids of strain G-MB1T were phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. The DNA G+C content of strain G-MB1T was determined to be 42.4 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain G-MB1T is separated from other Thalassomonas species. On the basis of the data presented, strain G-MB1T is considered to represent a novel species of the genus Thalassomonas, for which the name Thalassomonas fusca sp. nov. is proposed. The type strain is G-MB1T (=KCTC 32499T = NBRC 109830T). 相似文献
10.
Yi Li Hong Zhu Qiliang Lai Xueqian Lei Huajun Zhang Zhangran Chen Wei Zheng Yun Tian Hong Xu Tianling Zheng 《Antonie van Leeuwenhoek》2014,106(5):911-918
A Gram-stain negative, short rod-shaped aerobic bacterium with flagella, designated strain Y32T, was isolated from coastal seawater in Xiamen, Fujian Province of China. 16S rRNA gene sequence comparisons showed that strain Y32T is a member of the family Oceanospirillaceae, forming a distinct lineage with species of the genus Litoribacillus. The 16S rRNA gene sequence similarities between strain Y32T and other strains were all less than 94.0 %. Strain Y32T was found to grow optimally at 28 °C, at pH 7.0–8.0 and in the presence of 4–5 % (w/v) NaCl. The major fatty acids were identified as Summed Feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c, 49.4 %), C16:0 (17.7 %), C14:0 (6.9 %) and C18:1 ω9c (5.4 %). The major respiratory quinone was identified as ubiquinone-8 (Q-8). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain Y32T was determined to be 55.6 mol%. According to its morphology, physiology, fatty acid composition, polar lipids composition and 16S rRNA gene sequence data, strain Y32T represents a novel species of a new genus in the family Oceanospirillaceae, for which the name Litoribrevibacter albus gen. nov. sp. nov. is proposed. The type strain of Litoribrevibacter albus is Y32T (=MCCC 1F01211T=NBRC 110071T). 相似文献
11.
Shuang Geng Xin-Chi Pan Ran Mei Ya-Nan Wang Ji-Quan Sun Xue-Ying Liu Yue-Qin Tang Xiao-Lei Wu 《Current microbiology》2014,68(3):324-329
A Gram-negative, short rod-shaped, floc-forming bacterial strain J5-66T without any flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was capable of optimal growth at pH 7, 30 °C, and 1–2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the genus Ottowia in Comamonadaceae, and the highest 16S rRNA gene sequence similarity was 96.2 % with Ottowia pentelensis DSM 21699T. The major cellular fatty acids of strain J5-66T were C16:1 ω7c/C16:1 ω6c (45.0 %), C16:0 (21.1 %), C18:1 ω7c or/and C18:1 ω6c (19.2 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, glycolipid and two unidentified phospholipids (PL1 and PL2). The predominant ubiquinone was Q-8, and the G+C content of the genome DNA was 64.4 mol%. On the basis of genetic, phenotypic and chemotaxonomic analyses, strain J5-66T represents a novel species of the genus Ottowia for which the name Ottowia shaoguanensis sp. nov. is proposed. The type strain is J5-66T (=CGMCC 1.12431T =LMG 27408T). 相似文献
12.
Guanghua Wang Jiewei Fan Hualian Wu Xiaoyong Zhang Guangyu Li Hao Zhang Xi Yang Fangfang Ye Wenzhou Xiang Xiang Li 《Antonie van Leeuwenhoek》2013,104(6):933-939
A Gram-negative, aerobic, motile rod strain, designated Ma-20T, was isolated from a pool of marine Spirulina platensis cultivation, Sanya, China, and was subjected to a polyphasic taxonomy study. Strain Ma-20T can grow in the presence of 0.5–11 % (w/v) NaCl, 10–43 °C and pH 6–10, and grew optimally at 30 °C, pH 7.5–9.0 in natural seawater medium. The polar lipids were composed of phosphatidylethanolamine, three unidentified phospholipids and three unidentified polar lipids. The respiratory quinone was ubiquinone 8 (Q-8) and the major fatty acids were C18:1ω6c/C18:1ω7c (summed feature 8, 32.84 %), C16:1ω6c/C16:1ω7c (summed feature 3, 30.76 %), C16:0 (13.54 %), C12:03-OH (4.63 %), and C12:0 (4.09 %). The DNA G+C content of strain Ma-20T was 58 mol %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ma-20T belonging to Gammaproteobacteria, it shared 88.46–91.55 and 89.21–91.26 % 16S rRNA gene sequence similarity to the type strains in genus Hahella and Marinobacter, respectively. In addition to the large 16S rRNA gene sequence difference, Ma-20T can also be distinguished from the reference type strains Hahella ganghwensis FR1050T and Marinobacter hydrocarbonoclasticus sp. 17T by several phenotypic characteristics and chemotaxonomic properties. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain Ma-20T is suggested to represent a novel species of a new genus in Gammaproteobacteria, for which the name Nonhongiella spirulinensis gen. nov., sp. nov. is proposed. The type strain is Ma-20T (=KCTC 32221T=LMG 27470T). 相似文献
13.
Young-Ok Kim Sooyeon Park Bo-Hye Nam Yong-Taek Jung Dong-Gyun Kim Jung-Hoon Yoon 《Antonie van Leeuwenhoek》2013,104(6):1021-1027
A Gram-negative, non-spore-forming, aerobic, motile and rod-shaped or ovoid bacterial strain, designated MA1-3T, was isolated from a sea squirt (Halocynthia roretzi) collected from the South sea in South Korea. Strain MA1-3T was found to grow optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain MA1-3T fell within the clade comprising Colwellia species, clustering coherently with the type strains of Colwellia aestuarii, Colwellia polaris and Colwellia chukchiensis, showing sequence similarity values of 97.2, 96.4 and 95.6 %, respectively. It exhibited 16S rRNA gene sequence similarity values of 93.9–96.1 % to the type strains of the other Colwellia species. Strain MA1-3T was found to contain Q-8 as the predominant ubiquinone and C16:1 ω7c and/or C16:1 ω6c, C16:0 and C16:1 ω9c as the major fatty acids. The DNA G+C content of strain MA1-3T was determined to be 39.1 mol% and its mean DNA–DNA relatedness value with the type strain of C. aestuarii was 13 ± 5.4 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that the novel strain is separated from other Colwellia species. On the basis of the data presented, strain MA1-3T is considered to represent a novel species of the genus Colwellia, for which the name Colwellia meonggei sp. nov. is proposed. The type strain is MA1-3T (=KCTC 32380T = CECT 8302T). 相似文献
14.
Xue-Feng Jin Jin-Kwang Kim Qing-Mei Liu Myung-Suk Kang Dan He Feng-Xie Jin Sun-Chang Kim Wan-Taek Im 《Antonie van Leeuwenhoek》2013,103(6):1359-1367
A Gram-negative, strictly aerobic, non-motile, non-spore-forming and rod-shaped bacterial strain designated KHI67T was isolated from sediment of the Gapcheon River in South Korea and its taxonomic position was investigated by using a polyphasic approach. Strain KHI67T was observed to grow optimally at 25–30 °C and at pH 7.0 on nutrient and R2A agar. On the basis of 16S rRNA gene sequence similarity, strain KHI67T was shown to belong to the family Sphingomonadaceae and was related to Sphingomonas faeni MA-olkiT (97.6 % sequence similarity), Sphingomonas aerolata NW12T (97.5 %) and Sphingomonas aurantiaca MA101bT (97.3 %). The G + C content of the genomic DNA was determined to be 65.6 %. The major ubiquinone was found to be Q-10, the major polyamine was identified as homospermidine and the major fatty acids identified were summed feature 8 (comprising C18:1 ω7c/ω6c; 37.0 %), C16:0 (13.0 %), summed feature 3 (comprising C16:1 ω7c/C16:1 ω6c; 12.8 %) and C14:0 2OH (9.3 %). DNA and chemotaxonomic data supported the affiliation of strain KHI67T to the genus Sphingomonas. The DNA–DNA relatedness values between strain KHI67T and its closest phylogenetic neighbours were below 15 %. Strain KHI67T could be differentiated genotypically and phenotypically from the recognised species of the genus Sphingomonas. The isolate therefore represents a novel species, for which the name Sphingomonas ginsenosidivorax sp. nov. is proposed, with the type strain KHI67T (=KACC 14951T = JCM 17076T = LMG 25801T). 相似文献
15.
Zhi-Qing You Jie Li Sheng Qin Xin-Peng Tian Fa-Zuo Wang Si Zhang Wen-Jun Li 《Antonie van Leeuwenhoek》2013,103(5):963-969
A Gram-positive bacterium, designated SCSIO 15042T, was isolated from a sediment of the South China Sea and was subjected to a polyphasic taxonomic study. The isolate grew at 20–60 °C, pH 6.0–10.0 and it could grow with up to 10 % (w/v) NaCl. The cell-wall diamino acid was found to be meso-diaminopimelic acid. Polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and an unknown polar lipid. The only menaquinone was determined to be MK-7. The major fatty acids were identified as C16:1 ω7c/C16:1 ω6c, C16:0, iso-C15:0, anteiso-C15:0, and iso-C16:0. The DNA G+C content of strain SCSIO 15042T was determined to be 43.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain SCSIO 15042T to the genus Bacillus. Levels of 16S rRNA gene sequence similarities between strain SCSIO 15042T and Bacillus herbersteinensis D-1-5aT, Bacillus infantis SMC 4352-1T, Bacillus novalis LMG 21837T and Bacillus drentensis LMG 21831T were 96.2, 96.2, 96.1 and 96.1 %, respectively. Based on the evidence of the present polyphasic study, strain SCSIO 15042T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus abyssalis sp. nov. is proposed. The type strain is SCSIO 15042T (=DSM 25875T = CCTCC AB 2012074T = NBRC 109102T). 相似文献
16.
A new bacterial strain, designated as FF42T, was isolated from feces of domestic pigs—collected from Suwon, Korea—and was characterized to determine its taxonomic position. Strain FF42T was observed to be Gram negative, aerobic, non-spore forming, motile, and rod-shaped cells. Based on the phylogenetic and 16S rRNA sequence analyses, it was revealed that strain FF42T belonged to the genus Comamonas. The highest degree of sequence similarities was determined to be with Comamonas zonglianii BF-3T (96.3 %), Comamonas composti CC-YY287T (96.1 %), and Comamonas nitrativorans 23310T (95.9 %), while showing less than 95.6 % identity with the remaining Comamonas species. Growth of strain FF42T occurred between 25 and 40 °C (optimum, 28 °C) and at pH of 5-9 (optimum, pH 6.0). It grew in the presence of 0–3 % (w/v) NaCl while minimally tolerating at 3 % (w/v) NaCl. Biochemical and physiological tests revealed phenotypic differentiation of strain FF42T to other members of the genus Comamonas. The predominant quinone is ubiquinone (Q-8). The major cellular fatty acids were C10:0 3OH, C16:0, summed feature 3 (C16:1 ω7c/C16:1 ω6c), and summed feature 8 (C18:1 ω6c/C18:1 ω7c), all of which have previously been reported to occur in the species of the genus Comamonas. The G+C molar content for strain FF42T is 60.2 mol %. Based on phylogenetic and phenotypic analyses, strain FF42T (=KEMC 1002-058T=JCM 17561T) is clearly referred to be a novel species for the genus Comamonas, for which the name Comamonas faecalis sp. nov. is proposed. 相似文献
17.
Sang Hyeon Jeong Jong Hoon Lee Ji Young Jung Se Hee Lee Moon Su Park Che Ok Jeon 《Antonie van Leeuwenhoek》2013,103(3):503-512
Two moderately halophilic, facultatively aerobic, motile bacteria with flagella, designated strains 10-C-3T and 30-C-3, were isolated from jeotgal, a traditional Korean fermented seafood. Cells of the strains were observed to be ovoid-rods showing catalase- and oxidase-positive reactions and production of creamy-pink pigments. Growth of strain 10-C-3T was observed at 15–35 °C (optimum, 25–30 °C), at pH 5.5–9.0 (optimum, pH 7.0–7.5), and in the presence of 3–15 % (w/v) salts (optimum: 5–10 %). The two strains were found to contain C18:1 ω7c, C16:0, summed feature 3 (as defined by the MIDI system, comprising C16:1 ω7c and/or C16:1 ω6c), and C12:0 3-OH as the major cellular fatty acids. The G+C contents of the genomic DNA of strains 10-C-3T and 30-C-3 were determined to be 63.2 and 63.1 mol%, respectively and the respiratory quinone detected was ubiquinone 9 (Q-9) only. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains 10-C-3Tand 30-C-3 formed a distinct phyletic lineage within the genus Halomonas and are most closely related to Halomonas fontilapidosi 5CRT with 95.2 % of 16S rRNA sequence similarity. Strains 10-C-3Tand 30-C-3 shared 99.2 % of 16S rRNA gene sequence similarity and their DNA–DNA relatedness value was 96.6 ± 0.9 %. On the basis of phenotypic, chemotaxonomic and molecular features, strains 10-C-3Tand 30-C-3 represent a novel species of the genus Halomonas, for which the name Halomonas cibimaris sp. nov. is proposed. The type strain is 10-C-3T (= KACC 14932T = JCM 16914T). 相似文献
18.
Chongping Li Qiliang Lai Guizhen Li Chunming Dong Jianning Wang Yueting Liao Zongze Shao 《Antonie van Leeuwenhoek》2014,106(4):657-665
Two Gram-negative, non-spore-forming, oval to pear shaped motile strains, designated 25B14_1T and BH-BN04-4T, isolated from surface seawater from the Bering Sea and Chukchi Sea, respectively, were subjected to polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strains 25B14_1T and BH-BN04-4T clustered together with Hyphomonas atlanticus 22II1-22F38T and Hyphomonas oceanitis DSM 5155T, respectively, within genus Hyphomonas. Based on whole genome sequence analysis, the calculated DDH and ANIm values between strain 25B14_1T and BH-BN04-4T are 18.8 and 83.19 % respectively. The calculated DDH values of strain 25B14_1T and BH-BN04-4T with seven type strains ranged from 18.2 to 19.9 % and from 18.4 to 40.4 %, respectively. The ANIm values of strain 25B14_1T and BH-BN04-4T with seven type strains ranged from 83.00 to 84.67 % and from 83.14 to 90.58 %, respectively. Both isolates were found to contain Q-11 as the predominant respiratory quinone. The major fatty acids of strain 25B14_1T were identified as C16:0, C17:0, C18:1 ω7c-methyl and Summed Feature 8 (C18:1 ω6c/ω7c as defined by MIDI), while in the case of strain BH-BN04-4T they were identified as C16:0, C18:1 ω7c-methyl and Summed Feature 8 (C18:1 ω6c/ω7c). The G+C contents of 25B14_1T and BH-BN04-4T were determined to be 58.4 and 61.0 mol%, respectively. The combined phenotypic and genotypic data show that the two isolates each represent novel species of the genus Hyphomonas, for which the names Hyphomonas beringensis sp. nov. and Hyphomonas chukchiensis sp. nov. are proposed, with the type strain 25B14_1T (=MCCC 1A07321T = LMG 27914T) and BH-BN04-4T (=MCCC 1A07481T = LMG 27915T), respectively. 相似文献
19.
Yi Chen Zilian Zhang Yingnan Fu Yanan Wang Yu Wang Nianzhi Jiao 《Antonie van Leeuwenhoek》2013,104(6):1151-1157
A novel chemoheterotrophic, aerobic, Gram-negative, rod-shaped, yellow-pigmented, bacterial strain JLT2010T was isolated from deep seawater of the South China Sea. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain JLT2010T belongs to the family Flavobacteriaceae and is most closely related to Ulvibacter antarcticus IMCC3101T with 95.7 % similarity. Some phenotypic characteristics such as the absence of flexirubin-type pigments, growth at 37 °C, hydrolysis of casein differentiated strain JLT2010T from the genus Ulvibacter as well as other genera in the family Flavobacteriaceae. The DNA G+C content of the strain JLT2010T was found to be 35.7 mol% and the major respiratory quinone was found to be MK-6. On the basis of phenotypic and phylogenetic features, JLT2010T is classified as a novel genus and species within the family Flavobacteriaceae, for which the name Altuibacter lentus gen. nov., sp. nov. is proposed. The type strain is JLT2010T (=JCM 18884T = CGMCC 1.12167T). 相似文献
20.
Lei Zhang Lingfang Zhu Meiru Si Changfu Li Liang Zhao Yahong Wei Xihui Shen 《Antonie van Leeuwenhoek》2014,106(2):279-285
A white-coloured bacterium, designated strain GTJR-20T, was isolated from a stem of Phytolacca acinosa Roxb. collected from Taibai Mountain in Shaanxi Province, north-west China, and was subjected to a taxonomic study by using a polyphasic approach. The novel isolate was found to grow optimally at 28–30 °C, at pH 7.5–8.0 and in the absence of NaCl. Cells were observed to be Gram-stain positive, strictly aerobic, rod-shaped and non-motile. The predominant respiratory quinone was identified as MK-7(H4) and the major cellular fatty acids were identified as iso-C16:0 (35.8 %), C18:1 ω9c (17.7 %), C17:1 ω6c (11.0 %), C17:1 ω8c (7.8 %) and C18:3 ω6c (6, 9, 12) (7.2 %). The DNA G+C content was determined to be 71.6 mol %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GTJR-20T is a member of the genus Solirubrobacter and is closely related to Solirubrobacter phytolaccae GTGR-8T (16S rRNA gene sequence similarity, 98.4 %), Solirubrobacter soli KCTC 12628T (97.8 %), Solirubrobacter pauli KCTC 9974T (97.7 %) and Solirubrobacter ginsenosidimutans KCTC 19420T (97.6 %). No other recognized bacterial species showed more than 94.6 % 16S rRNA gene sequence similarity to the novel isolate. DNA–DNA relatedness values for strain GTJR-20T with respect to its closely related neighbours S. phytolaccae GTGR-8T, S. soli KCTC 12628T, S. pauli KCTC 9974T and S. ginsenosidimutans KCTC 19420T were 48.3 ± 8.6, 21.3 ± 5.2, 36.8 ± 6.2 and 36.0 ± 5.5 %, respectively. Based on the phenotypic, phylogenetic and genotypic data, strain GTJR-20T is considered to represent a novel species of the genus Solirubrobacter, for which the name Solirubrobacter taibaiensis sp. nov. is proposed. The type strain is GTJR-20T (=CCTCC AB 2013308T = KCTC 29222T). 相似文献