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The murine liver-specific nonclassical MHC class I molecule Q10 binds a classical peptide repertoire
Zappacosta F Tabaczewski P Parker KC Coligan JE Stroynowski I 《Journal of immunology (Baltimore, Md. : 1950)》2000,164(4):1906-1915
The biological properties of the nonclassical class I MHC molecules secreted into blood and tissue fluids are not currently understood. To address this issue, we studied the murine Q10 molecule, one of the most abundant, soluble class Ib molecules. Mass spectrometry analyses of hybrid Q10 polypeptides revealed that alpha1alpha2 domains of Q10 associate with 8-9 long peptides similar to the classical class I MHC ligands. Several of the sequenced peptides matched intracellularly synthesized murine proteins. This finding and the observation that the Q10 hybrid assembly is TAP2-dependent supports the notion that Q10 groove is loaded by the classical class I Ag presentation pathway. Peptides eluted from Q10 displayed a binding motif typical of H-2K, D, and L ligands. They carried conserved residues at P2 (Gly), P6 (Leu), and Pomega (Phe/Leu). The role of these residues as anchors/auxiliary anchors was confirmed by Ala substitution experiments. The Q10 peptide repertoire was heterogeneous, with 75% of the groove occupied by a multitude of diverse peptides; however, 25% of the molecules bound a single peptide identical to a region of a TCR V beta-chain. Since this peptide did not display enhanced binding affinity for Q10 nor does its origin and sequence suggest that it is functionally significant, we propose that the nonclassical class I groove of Q10 resembles H-2K, D, and L grooves more than the highly specialized clefts of nonclassical class I Ags such as Qa-1, HLA-E, and M3. 相似文献
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David T. Evans Marian S. Piekarczyk Luis Cadavid Virginia S. Hinshaw D. I. Watkins 《Immunogenetics》1998,47(3):206-211
The products of the highly polymorphic and variable major histocompatibility complex (MHC) class I loci play a crucial role
in host defenses against infectious disease. While similar alleles have been found in closely related species, sharing of
a functional MHC class I allele between two species has never been reported. Here we show that an identical functional MHC
class I molecule is present in two different primate species with an approximate divergence time of 0.7 million years. Lymphocytes
from the red-crested tamarin (Saguinus geoffroyi) expressed an MHC class I allele (Sage-G
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01) that was identical in coding sequence to an MHC class I allele (Saoe-G
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08) found in the cotton-top tamarin (Saguinus oedipus). Furthermore, influenza virus-specific cytotoxic T lymphocytes (CTLs) generated in the cotton-top tamarin killed lymphocytes
expressing the influenza virus nucleoprotein (NP) from the red-crested tamarin. Since the influenza virus NP epitope is bound
by Saoe-G*08 in the cotton-top tamarin, it is likely that this molecule is functional in both species. These data provide the first
evidence that functional MHC class I molecules can be maintained entirely intact in two separate species.
Received: 6 June 1997 / Revised: 21 July 1997 相似文献
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Cytotoxic T lymphocytes from mice with soluble class I Q10 molecules in their serum are not tolerant to membrane-bound Q10 总被引:2,自引:0,他引:2
D W Mann I Stroynowski L Hood J Forman 《Journal of immunology (Baltimore, Md. : 1950)》1987,138(1):240-245
Q10 is a class I Qa-2 region-encoded molecule that is secreted by the liver and present in serum at high concentrations (about 10 to 60 micrograms/ml) in most strains of mice. The amino terminal portion of this molecule can also be expressed as an integral membrane protein by splicing the 5' end of the Q10 gene to the 3' end of H-2Ld and transfecting the hybrid gene into murine L cells. Because CTL primarily recognize polymorphic determinants controlled by the alpha 1 and alpha 2 domains of class I molecules and because the Q10d/Ld product expressed by transfected L cells includes the alpha 1 and alpha 2 domains of Q10d, we could address whether mice bearing serum Q10 were tolerant to this molecule at the CTL level. The results of these experiments demonstrate that Q10+ mice are able to generate H-2-unrestricted CTL activity against Q10d expressed on transfected L cells, and this response was not inhibitable by the addition of Q10-containing normal mouse serum. It is unlikely that this CTL activity is due to possible polymorphic differences in Q10 alleles, since semisyngeneic BALB/c (H-2d) mice, from which the Q10d hybrid gene construct was derived, are able to generate anti-Q10d effector cells. The Q10d molecule was shown to cross-react with H-2Ld, lending support to the concept that Qa genes can serve as donors for polymorphic sequences found in H-2K, -D, and -L. That mice can generate anti-Q10 CTL activity suggests that this soluble class I protein does not act as a toleragen for these cells. The implications of these findings for an understanding of self-tolerance are discussed. 相似文献
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Individual MHC class I and MHC class IIB diversities are associated with male and female reproductive traits in the three-spined stickleback 总被引:1,自引:0,他引:1
Jäger I Eizaguirre C Griffiths SW Kalbe M Krobbach CK Reusch TB Schaschl H Milinski M 《Journal of evolutionary biology》2007,20(5):2005-2015
Genes of the major histocompatibility complex (MHC) are indispensable for pathogen defence in vertebrates. With wild-caught three-spined sticklebacks (Gasterosteus aculeatus) we conducted the first study to relate individual reproductive parameters to both MHC class I and II diversities. An optimal MHC class IIB diversity was found for male nest quality. However, male breeding colouration was most intense at a maximal MHC class I diversity. One MHC class I allele was associated with male redness. Similarly, one MHC class IIB allele was associated with continuous rather than early female reproduction, possibly extending the reproductive period. Both alleles occurred more frequently with increasing individual allele diversity. We suggest that if an allele is currently not part of the optimum, it had not been propagated by choosy females. The parasite against which this allele provides resistance is therefore unlikely to have been predominant the previous year - a step to negative frequency-dependent selection. 相似文献
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Structure and expression of a chicken MHC class I gene 总被引:7,自引:0,他引:7
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