Ecoregions as predictors of lotic assemblages of blackflies (Diptera: Simuliidae)

Local ecological attributes of streams are known to have strong influences on community membership for many aquatic insects. Differences in aquatic insect assemblages, therefore, should be clearly detectable across large scale ecological "units", such as ecoregions. Many studies of aquatic invertebrates however, have suffered from a lack of species level identifications. In addition, many previous studies that examined the influence of ecoregion on aquatic assemblages have the implicit assumption that members of different taxa are responding in the same manner. Our study, therefore, was restricted to an ecologically (lotic) and functionally (mostly filter-feeding) homogenous group, the Simuliidae. In the current study, we examine the relationship between species assemblages of preimaginal blackflies and the landscape through which their stream habitats flow. Accordingly, the larval simuliid faunas from South Carolina, USA, are compared among three ecoregions established a priori: Blue Ridge Mountains, Piedmont, and Sandhills. Using discriminant function analysis, we show that each ecoregion produces a distinct stream habitat; factors responsible for regionalization are quantified. We also show that streams can be assigned correctly to ecoregion of origin 85% of the time on the basis of the simuliid assemblage. We suggest that our results can be interpreted most readily by considering the distribution of individual species.     

Do isolation and local habitat jointly limit the structure of stream invertebrate assemblages?

1. Both local and regional processes simultaneously control species assemblages depending on spatial habitat configuration. In dendritic networks like streams, the unique spatial arrangement of habitats produces various combinations of local habitat size and isolation. Stream invertebrate assemblages could therefore be controlled by different combinations of local and regional processes, depending on their location in the network. 2. Using quantile regression, we investigated how local habitat size, local environmental conditions and spatial isolation influenced variation in assemblage composition. Adult Trichoptera and benthic macroinvertebrate assemblages were represented by non‐metric multidimensional scaling (NMDS) ordination scores, as were local environmental conditions, in four headwater stream networks in New Zealand. 3. With increasing local habitat size, there was a decrease in variation in assemblage composition (NMDS scores) of both adult Trichoptera and benthic macroinvertebrates. This relationship between habitat size and assemblage variation was related to local habitat conditions at the upper limit of assemblage variability and spatial isolation at the lower limit of assemblage variability, for both adult Trichoptera and benthic assemblages, indicating joint local and regional controls on stream invertebrate assemblages. 4. The relationships between local assemblages and their neighbours, based on community similarity scores, differed between benthic macroinvertebrates and adult Trichoptera. For benthic assemblages, the larger the stream, the more similar assemblages were to neighbouring assemblages, whereas there was no consistent relationship between assemblage similarity and stream size for adult Trichoptera. This difference in structuring could be attributed to contrasting spatial influences linked to the different dispersal modes of adults and larvae. However, because adult and benthic assemblages are not independent, the influence of life stage on spatial distribution is difficult to determine (i.e. it is essentially a ‘chicken and egg’ argument). 5. Overall, our approach using quantile regression to evaluate limit responses, rather than regressions on means, has highlighted the joint importance of local habitat and spatial processes in structuring stream invertebrate assemblages. Furthermore, we have provided evidence for the importance of the spatial network arrangement and interactions between life stages and dispersal processes, in structuring stream assemblages.     

Patterns of species richness for blackflies (Diptera: Simuliidae) in the Nearctic and Neotropical regions

Abstract.  1. Patterns of simuliid species richness were examined over a variety of scales at 532 stream sites in the Nearctic (394) and Neotropical (138) regions. In Nearctic streams, species richness of immature blackflies both within and across ecoregions and over two seasons was examined. Stream variables at each site included seston, width, depth, velocity, discharge, conductivity, pH, dissolved oxygen, water temperature, dominant streambed-particle size, canopy cover, and riparian vegetation. These variables were subjected to a principal component analysis and derived principal components were related back to richness, using regression analysis. At the level of the stream reach, richness was not highly correlated with single-point measurements of stream conditions.
2. Using data from both Nearctic and Neotropical sites, the effect of regional richness on local richness was examined. As regional richness increased, local diversity reached an asymptote in which further increases in regional richness were not matched by increases in local richness. Hence, simuliid communities are best described as saturated (type II) communities, consistent with the current view of lotic communities as non-equilibrium systems.
3. The well-established pattern of greater species richness in tropical regions was not observed in this study. To the contrary, blackfly richness is higher in temperate streams than in tropical streams at both local and regional scales.     

Flow intermittency alters longitudinal patterns of invertebrate diversity and assemblage composition in an arid‐land stream network

1. Temporary streams comprise a large proportion of the total length of most stream networks, and the great majority of arid‐land stream networks, so it is important to understand their contribution to biotic diversity at both local and landscape scales. 2. In late winter 2010, we sampled invertebrate assemblages in 12 reaches of a large arid‐land stream network (including perennial and intermittent headwaters, intermittent middle reaches and perennial rivers) in south‐east Arizona, U.S.A. Intermittent reaches had then been flowing for c. 60 days, following a dry period of more than 450 days. We sampled a subset of the perennial study reaches three more times between 2009 and 2011. Since intermittent reaches were dry during these additional sampling periods, we used assemblage data from two other intermittent streams in the study network (sampled in 2004–05 and 2010) to explore interannual variability in intermittent stream assemblage composition. 3. Invertebrate richness was lowest in intermittent reaches, despite their often being connected to species‐rich perennial reaches. The assemblages of these intermittent reaches were not simply a subset of the species in perennial streams, but rather were dominated by a suite of stoneflies, blackflies and midges with adaptations to intermittency (e.g. egg and/or larval diapause). On average, 86% of individuals in these samples were specialists or exclusive to intermittent streams. Predators were 7–14 times more abundant in perennial than in intermittent reaches. 4. Despite being separated by long distances (12–25 km) and having very different physical characteristics, the assemblages of perennial headwaters and rivers were more similar to one another than to intervening intermittent reaches, emphasising the prime importance of local hydrology in this system. 5. The duration and recurrence intervals of dry periods, and the relative importance of dispersal from perennial refuges, probably influence the magnitude of biological differences between neighbouring perennial and temporary streams. Although perennial headwaters supported the highest diversity of invertebrates, intermittent reaches supported a number of unique or locally rare species and as such contribute to regional species diversity and should be included in conservation planning.     

Species richness and assemblage structure of Trichoptera in Danish streams

• 1 Species richness and assemblages of Trichoptera from 157 ‘pristine’ Danish lowland stream sites were analyzed, for dependence on geographical position of the sites and simple physical variables, using two way indicator species analysis and detrended correspondence analysis.
• 2 A total of 106 species were recorded, representing ≈ 90% of the species pool known from Danish streams. Only seven species occurred at more than half the sites, whereas an additional 11 species were found at more than a quarter of the sites.
• 3 Although sites showed significant regional differences in environmental variables (stream order, width, slope and presence/absence of riparian forest), species richness and assemblages were primarily correlated with stream order, width and slope. Maximum richness was found at the largest (5th order) stream sites.
• 4 Regional differences in species assemblages were found, with several species being absent from the islands Funen and Bornholm. Species assemblages also differed between forested and non‐forested stream sites.
• 5 We found evidence that stream temperature may be of only minor importance in determining Trichoptera species richness and assemblage composition in Danish streams compared to other size‐related physical factors.

     

Effects of local and regional factors on the fish assemblage structure in Meridional Amazonian streams

The aim of this study was to evaluate the effects of local, regional and temporal factors structuring fish assemblages in Meridional Amazonian streams during the months of May (rainy season) and August (dry season) of 2008. To accomplish this task, 14 streams located in Serra do Expedito (Aripuanã River basin) were sampled along 30-m stretches. A total of 3,212 specimens distributed among five orders, 18 families, and 55 species were recorded. The fish assemblage structure in the streams presented variation among types of riparian vegetation (local factor) and watersheds (regional factor), but did not present variation between seasons (temporal factor) and stream order (regional factor). Larger streams with margins covered with pasture presented higher species richness and abundance than smaller streams with margins covered with forest.     

Convergence of temperate and tropical stream fish assemblages

The hypothesis of convergence takes the deterministic view that community (or assemblage) structure can be predicted from the environment, and that the environment is expected to drive evolution in a predictable direction. Here we present results of a comparative study of freshwater fish assemblages from headwater streams in four continents (Europe, North America, Africa and South America), with the general objective of testing whether these assemblages display convergent structures under comparable environmental conditions (i.e. assemblage position in the stream longitudinal continuum). We tested this hypothesis by comparing species richness and trophic guilds of those stream fish assemblages represented in available data from multiple sites on each continent. Independent of phylogenetic and historical constraints, fish assemblage richness and trophic structure in the four continents converged along the stream continua to a substantial degree. For the four continents, assemblage richness increased, the proportion of invertivorous species decreased, and the proportion of omnivorous species increased from upstream to downstream, supporting theoretical predictions of the river continuum concept. However, the herbivore/detritivore and piscivore guilds were virtually absent from our small European and North American stream sites, unlike our African and South American stream sites. This divergence can be linked to differences in energy availability between temperate and tropical systems.     

Environmental variability drives phytoplankton assemblage persistence in a subtropical reservoir

Temporal changes of assemblages may result from environmental variability and reflect seasonal dynamics of their ecosystem. In the subtropics, the hydrological regime is usually characterized by well‐defined wet and dry seasons, regulating discharge and influencing a series of environmental variables that affect phytoplankton persistence. Therefore, we may expect that dry seasons are environmentally more stable than wet seasons. We analysed interannual phytoplankton assemblage variability (or, inversely, persistence) in a subtropical reservoir sampled every austral summer and winter during 5 years. We tested (i) if phytoplankton assemblage structure differed between the dry (summer) and wet (winter) seasons; (ii) if assemblage persistence differed between the seasons; (iii) if assemblage persistence was related to environmental stability; and (iv) if assemblage dissimilarity increased over time. Phytoplankton assemblages differed between the summer and winter seasons. Winter indicator species were mostly Bacillariophyceae or Cryptophyceae, whereas Cyanophyceae and Chlorophyceae taxa were more frequent and abundant in summer. Assemblages in the dry season were more persistent among years than those occurring during rainy periods. Similarly, environmental variability tended to be lower among dry than among rainy seasons. The relation between the phytoplankton temporal cycle and the temporal patterns of environmental variability supports our prediction that high environmental stability results in more persistent assemblages. Assemblage dissimilarity increased as sampling years were farther apart, for both seasons. Additionally, assemblages in the rainy periods showed a more pronounced increase in dissimilarity, as their changes among years were less predictable. We found a clear temporal pattern and an increased dissimilarity over time in the phytoplankton assemblage structure. Unravelling these temporal patterns may improve our understanding of phytoplankton temporal dynamics, and may have implications for management and monitoring programs. High dissimilarity of assemblages among years, particularly among rainy periods, can obscure human impacts, and monitoring programs should take this into account.     

Effects of multi-year droughts on fish assemblages of seasonally drying Mediterranean streams

1. This study analysed changes occurring in Mediterranean stream fish assemblages over a sequence of dry years followed by a generally wet period (1991–98). Variations in assemblage attributes were quantified at the basin and stream reach scales, and related to variables reflecting the occurrence of unusually dry or wet conditions. 2. Assemblage variability increased along with the resolution of analysis, with little changes in species richness, composition and rank abundances, but significant variation in individual species abundances. Fluctuations in relative abundances were significantly affected by variables reflecting the severity of summer droughts and the occurrence of rainy springs. These patterns were evident at the basin scale, while variability at individual stream reaches tended to be higher and less related to rainfall patterns. 3. At least three response guilds to rainfall variation could be identified: two of the four abundant and widespread species (chub and loach) declined following dry years, whereas the two other core species (nase and eel) declined after rainy spring; one scarce native species (stickleback) increased in dry years. 4. Except at the two most upstream reaches, the assemblages tended to recover quickly to previous configuration after the changes occurring during the sequence of dry years. 5. Temporal variability of local assemblages was concordant among reaches but did not follow any consistent spatial pattern. Instead, spatial patterns in assemblage attributes changed over time in response to environmental variability, with a tendency for a disruption of upstream–downstream gradients following dry years. 6. Results supported the view that present‐day droughts cause relatively small and transient changes to Mediterranean stream fish assemblages. However, longer and more severe droughts expected under altered future climates, may result in declines or local extinctions of the most sensitive species and their potential replacement by more resistant species. Changing drought regimes thus need to be duly considered in the development of conservation strategies for Mediterranean stream fish.     

Spatial and temporal patterns in fish assemblages of upper coastal plain streams, Mississippi, USA

We assessed spatial, seasonal, and annual variation in fish assemblages over 17 months in three small- to medium-sized, incised streams characteristic of northwestern Mississippi streams. We sampled 17 962 fish representing 52 species and compared assemblages within and among streams. Although annual and seasonal variability in assemblage structure was high, fish assemblages maintained characteristics unique to each stream. High variability in fish catch-per-unit-effort (CPUE) was exemplified in one site where total CPUE increased an order of magnitude from July 1993 to 1994. Species turnover and percent dissimilarity were often higher seasonally than annually, consistent with a period of change in spring to early summer and a return to similar species compositions between summers. Temporal variability was also high at the individual species level, and no species were classified as 'stable'. We found little evidence for correlation between changes in fish assemblage structure and measured habitat conditions. The fish characteristics fit the profile of 'colonizing assemblages', which probably resulted from both natural and anthropogenic causes. Flashy hydrographs , created in part by stream channelization and incision and watershed deforestation, may play a large role in structuring these fish assemblages. Extreme interannual variability in assemblages in the absence of detectable habitat change has important implications for the statistical power of fish monitoring programs designed to detect trends in fish assemblages over time.     

Nutrient recycling by fish in streams along a gradient of agricultural land use

Nutrient recycling is an essential ecosystem process provided by animals. In many aquatic systems, fish have been identified as important in ecosystem nutrient recycling; however, this importance can vary widely between systems. The factors controlling intersystem variation in animal‐mediated nutrient cycling have rarely been examined and as such it remains unclear what impact human landscape changes will have upon these processes. Here we examined rates of nutrient recycling for temperate stream fish assemblages along a gradient of agricultural land use (proportion cropland in the watershed: 1–59%). We quantified nutrient excretion rates of both ammonium–N (NH₄⁺–N) and phosphate (as soluble reactive phosphate: SRP) for fish assemblages at eight streams in southern Ontario, Canada with species‐specific excretion measurements and quantitative assemblage sampling. For both nutrients, total assemblage excretion exhibited a strong positive relationship with riparian cropland. The distance required for fish assemblages to turn over ambient nutrient pools was shorter for cropland systems, indicating that the relative importance of excreted nutrients was higher in these systems. Based on measured uptake rates of NH₄⁺–N in two streams (one higher cropland and one low cropland) and on modeled uptake rates for all streams, the proportion of ecosystem demand that can be satisfied by excretion is generally higher in the more agricultural streams. These patterns appear to be driven largely by disproportionate increases in fish assemblage biomass with increasing stream nutrient concentrations.     

Control of stream insect assemblages: roles of spatial configuration and local environmental factors

Abstract 1. Current views in ecology emphasise that community structure is the sum of multiple processes, with imprints of both regional and local drivers. However, the degree to which stream insect assemblages are structured by spatial configuration (complying with the dispersal‐based neutral hypothesis) and local environmental features (complying with the niche‐based species sorting hypothesis) has not been rigorously examined based on surveys in multiple years. 2. Stream sites in a boreal drainage system were surveyed during three consecutive years and the relative contribution of spatial configuration and local environmental variables to aquatic insect assemblage structure (characterised by both abundance and presence–absence data) was assessed. Separate analyses were conducted for mayflies (Ephemeroptera), stoneflies (Plecoptera), caddisflies (Trichoptera), and non‐biting midges (Diptera: Chironomidae) in each year. 3. There were no relationships between the spatial location and local environmental features of streams in Mantel tests, facilitating exploration of their independent effects on assemblage structure. The study found virtually no effects of spatial location on stream insect assemblages across the study drainage system, as evidenced by Mantel tests and canonical correspondence analyses (CCA). The environmental variables were also rather weakly associated with assemblage structure, with the total amount of explained variation ranging from 9.8% to 31.7% in the CCAs. There were no appreciable differences in the amount of environment‐related explained variation in assemblage structure between mayflies, stoneflies, caddisflies, and midges, but some between‐year differences were noticeable in most insect groups. The environmental variables that were significantly related to assemblage structure exhibited some between‐group and between‐year variability. In general, patterns shown by abundance and presence–absence data were highly similar. 4. It appears that stream insect assemblages comply with the niche‐based species sorting hypothesis in the context of metacommunity ecology. In contrast, the absence of spatial structuring suggests that stream insect assemblages do not comply with the neutral hypothesis, being not strongly dispersal limited at the within‐drainage basin scale.     

Seasonal dynamics of macroinvertebrate assemblages in the benthos and associated with detritus packs in two low-order streams with different riparian vegetation

• 1 The seasonal dynamics of the benthic macroinvertebrate assemblage, and the subset of this assemblage colonising naturally formed detritus accumulations, was investigated in two streams in south‐west Ireland, one draining a conifer plantation (Streamhill West) and the other with deciduous riparian vegetation (Glenfinish). The streams differed in the quantity, quality and diversity of allochthonous detritus and in hydrochemistry, the conifer stream being more acid at high discharge. We expected the macroinvertebrate assemblage colonising detritus to differ in the two streams, due to differences in the diversity and quantity of detrital inputs.
• 2 Benthic density and taxon richness did not differ between the two streams, but the density of shredders was greater in the conifer stream, where there was a greater mass of benthic detritus. There was a significant positive correlation between shredder density and detritus biomass in both streams over the study period.
• 3 Detritus packs in the deciduous stream were colonised by a greater number of macroinvertebrates and taxa than in the conifer stream, but packs in both streams had a similar abundance of shredders. The relative abundance of taxa colonising detritus packs was almost always significantly different to that found in the source pool of the benthos.
• 4 Correspondence analysis illustrated that there were distinct faunal differences between the two streams overall and seasonally within each stream. Differences between the streams were related to species tolerances to acid episodes in the conifer stream. Canonical correspondence analysis demonstrated a distinct seasonal pattern in the detrital composition of the packs and a corresponding seasonal pattern in the structure of the detritus pack macroinvertebrate assemblage.
• 5 Within‐stream seasonal variation both in benthic and detritus pack assemblages and in detrital inputs was of similar magnitude to the between‐stream variation. The conifer stream received less and poorer quality detritus than the deciduous stream, yet it retained more detritus and had more shredders in the benthos. This apparent contradiction may be explained by the influence of hydrochemistry (during spate events) on the shredder assemblage, by differences in riparian vegetation between the two streams, and possibly by the ability of some taxa to exhibit more generalist feeding habits and thus supplement their diets in the absence of high quality detritus.

     

Drought refuges,spatial scale and recolonisation by invertebrates in non‐perennial streams

1. If resistance traits drive recolonisation after drought, then drought refuges should contribute strongly to assemblage composition within streams. If resilience traits drive recolonisation, macroinvertebrates emerging from refuges may disperse widely, colonising many streams. To determine whether the contribution of drought refuges to macroinvertebrate recolonisation in non‐perennial streams was mostly local (within stream) or broader scale (across streams), we measured the association between the composition of invertebrate assemblages in different types of in‐stream drought refuge and the assemblage composition of streams when flow resumed. 2. We sampled 16 streams of varying hydrological regime on the western side of the Victoria Range in the Grampians National Park, Victoria, Australia. Drought refuges (perennial pools, dry sediment, damp sediment, seeps, patches of leaf litter, beneath stones) were identified and sampled during autumn. Most taxa were found in perennial pools; few taxa were found aestivating beneath stones or having desiccation‐resistant stages in dry sediment. Perennial pools and perennially flowing reaches were the refuges that harboured the greatest diversity of macroinvertebrate taxa. 3. Streams were sampled again during spring. Assemblage composition of non‐perennial reaches in spring was unrelated to composition in nearby refuges in the previous autumn. In contrast, assemblage composition in perennial reaches during spring was strongly correlated with composition during autumn. Therefore, drought refuges did not directly influence assemblage composition locally within non‐perennial streams. Rather, both perennially flowing reaches and perennial pools acted as drought refuges across the broader landscape. Resilience traits are likely to drive recolonisation in these streams. 4. Monitoring of drought refuges in a particular stream will therefore not predict species composition when flow resumes. Drought refuges are likely to sustain biodiversity over larger spatial scales such as groups of streams or whole drainage networks. Consequently, stream networks will need to be managed as entities rather than as single waterways and the focus of drought refuge protection should be on perennial pools and reaches.     

Loss of functionally important and regionally endemic species from streams forced into intermittency by global warming

Climate change is altering hydrological cycles globally, and in Mediterranean (med-) climate regions it is causing the drying of river flow regimes, including the loss of perennial flows. Water regime exerts a strong influence over stream assemblages, which have developed over geological timeframes with the extant flow regime. Consequently, sudden drying in formerly perennial streams is expected to have large, negative impacts on stream fauna. We compared contemporary (2016/17) macroinvertebrate assemblages of formerly perennial streams that became intermittently flowing (since the early 2000s) to assemblages recorded in the same streams by a study conducted pre-drying (1981/82) in the med-climate region of southwestern Australia (the Wungong Brook catchment, SWA), using a multiple before-after, control-impact design. Assemblage composition in the stream reaches that remained perennial changed very little between the studies. In contrast, recent intermittency had a profound effect on species composition in streams impacted by drying, including the extirpation of nearly all Gondwanan relictual insect species. New species arriving at intermittent streams tended to be widespread, resilient species including desert-adapted taxa. Intermittent streams also had distinct species assemblages, due in part to differences in their hydroperiods, allowing the establishment of distinct winter and summer assemblages in streams with longer-lived pools. The remaining perennial stream is the only refuge for ancient Gondwanan relict species and the only place in the Wungong Brook catchment where many of these species still persist. The fauna of SWA upland streams is becoming homogenised with that of the wider Western Australian landscape, as drought-tolerant, widespread species replace local endemics. Flow regime drying caused large, in situ alterations to stream assemblage composition and demonstrates the threat posed to relictual stream faunas in regions where climates are drying.     

Fish assemblage convergence along stream environmental gradients: an intercontinental analysis

Species that pass through similar environmental filters, regardless of geographic proximity or evolutionary history, are expected to share many traits, resulting in similar assemblage trait distributions. Convergence of assemblage trait distributions among different biotic regions would indicate that consistent ecological processes produce repeated patterns of adaptive evolution. This study analyzes trait–environment relationships across multiple stream fish assemblages representing evolutionarily divergent faunas. We hypothesized that trait–environment patterns converge across regional faunas in response to a common set of environmental filters acting on functional traits. One hundred and ninety‐seven species and forty streams were sampled from five regions: Belize, Benin, Brazil, Cambodia and USA. By examining trait–environment plots, multiple congruent trait–environment patterns were found across all regions, indicative of a consistent set of environmental filters acting on local community assembly. The consistency of these patterns strongly suggests that water velocity and habitat structural complexity function as universal environmental filters, producing similar assemblage trait distributions in streams across all regions. Bivariate relationships were not universal, and only one of the associations between a single functional trait and single environmental variable was statistically significant across all five regions. Strong phylogenetic signal was found in traits and habitat use, which implies that niche conservatism also influenced assemblage trait distributions. Overall, results support the idea that habitat templates structure trait distributions of stream fish assemblages and do so in a consistent manner.     

Effects of natural hydrological variability on fish assemblages in small Mediterranean streams: Implications for ecological assessment

Small Mediterranean streams are shaped by predictable seasonal events of flooding and drying over an annual cycle, and present a strong inter and intra-annual variation in flow regime. Native fish assemblages in these streams are adapted to this natural environmental variability. The distinction of human-induced disturbances from the natural ones is thus a crucial step before assessing the ecological status of these streams. In this aim, the present study evaluates the effects of natural hydrological variability on fish assemblages from disturbed and least disturbed sites in small intermittent streams of south Portugal. Data were collected over the last two decades (1996–2011) in 14 sites located in the Guadiana and Sado river basins. High variability of fish assemblages was strongly dependent on human-induced disturbances, particularly nutrient/organic load and sediment load, and on natural hydrological variability. Natural hydrological variability can act jointly with anthropogenic disturbances, producing changes on fish assemblages structure of small intermittent streams. In least disturbed sites, despite the natural disturbances caused by inter-annual rainfall variations (including drought and flood events), fish assemblages maintained a long-term stability and revealed a high resilience. On the contrary, disturbed sites presented significantly higher variability on fish assemblages and a short and long-term instability, reflecting a decrease on the resistance and resilience of fish assemblages. Under these conditions, fish fauna integrity is particularly vulnerable and the ecological assessment may be influenced by natural hydrological variations. High hydrological variability (especially if it entails high frequency of dryer years and meaningful cumulative water deficit) may affect the impact of the human pressures with significant and consistent consequences on fish assemblage composition and integrity. In this study, fish metrics that maximize the detection of human degradation and minimize the response to natural variability were based on the relative abundance of native species (insectivorous species, eurytopic species, water column species, native lithophilic species), relative abundance of species with intermediate tolerance and relative number of exotic species. Results highlight the importance of assessing temporal variability on stream biomonitoring programs and emphasize the need to improve the assessment tools, accounting for long-term changes in fish assemblages, namely by selecting the most appropriate fish metrics that respond to anthropogenic disturbances but exhibit low natural temporal variability, essential both in the characterization of the biological reference conditions and in the development of fish indexes in intermittent streams.     

Fishes of Indiana streams: current and historic assemblage structure

We examined Indiana fish assemblages using taxonomy and ecological categories to assess temporal shifts in community structure and recent environmental relationships. Historic (1945) and recent (1996–2007) presence/absence data were compiled by subbasin and analyzed with Nonmetric Multidimensional Scaling (NMS) ordination and by species richness. Canonical Correspondence Analysis (CCA) was used to test taxonomic identity and ecological category abundance data for explanation with recent (1996–2007) environmental variables. We found a decrease in assemblage heterogeneity for recent assemblages and an increase in the number of tolerant species per subbasin. Recent Indiana streams are dominated by tolerant fishes with generalist life history strategies and low functional variation. The use of ecological categories resulted in weaker relationships with environmental variables than analyses with taxonomic identities. Analyses using taxonomy resulted in strong assemblage explanation from stream size and flow variation, while analyses using ecological categories resulted in strong assemblage explanation from habitat variation in silt substrates and flow. Analyses of recent assemblage structure using ecological categories resulted in decreased assemblage variation among subbasins than in analyses using taxonomic identities. We found that fish assemblages of Indiana streams are structured primarily by habitat complexity and have been altered during the past 50 years through multiple disturbances including fragmentation, siltation, and species introductions.     

Effect of fire on benthic algal assemblage structure and recolonization in intermittent streams

Abstract: Dry biofilm on rocks and other substrata forms an important drought refuge for benthic algae in intermittent streams following the cessation of flow. This dry biofilm is potentially susceptible to disturbance from bushfires, including direct burning and/or scorching and damage from radiant heat, particularly when streams are dry. Therefore, damage to dry biofilms by fire has the potential to influence algal recolonization and assemblage structure in intermittent streams following commencement of flow. The influence of fire on benthic algal assemblages and recolonization was examined in intermittent streams of the Grampians National Park, Victoria, Australia, using a field survey and manipulative field experiment. The field survey compared assemblages in two intermittent streams within a recently burnt area (within 5 months of the fire) with two intermittent streams within an unburnt area. The two burnt streams were still flowing during the fire so most biofilms were not likely to be directly exposed to flames. Considerable site‐to‐site and stream‐to‐stream variation was detected during the field survey, which may have obscured potential differences attributable to indirect effects of the fire. The manipulative field experiment occurred in two intermittent streams and consisted of five treatments chosen to replicate various characteristics of bushfires that may influence dry biofilms: dry biofilm exposed directly to fire; dry biofilm exposed to radiant heat; dry biofilm exposed to ash; and two procedural controls. After exposure to the different treatments, rocks were replaced in the streams and algae were sampled 7 days after flow commenced. Differences occurred across treatments, but treatment differences were inconsistent across the two streams. For example, direct exposure to fire reduced the abundance of recolonizing algae and altered assemblage structure in both streams, while radiant heat had an effect on assemblage structure in one stream only. The manipulative field experiment is likely to have represented the intensity of a small bushfire only. Nonetheless, significant differences across treatments were detected, so these experimental results suggest that fire can damage dry biofilms, and hence, influence algal recolonization and assemblage structure in intermittent streams.     

Seasonal variation in the composition of fish assemblages in small Amazonian forest streams: evidence for predictable changes

1. The effects of seasonal inundation on the biology of fishes on floodplains of large Amazonian rivers are well studied. However, the small seasonal changes in headwater streams are generally considered to have little effect on fish assemblages. 2. In this study, we analysed seasonal changes in the species composition and abundance of fish in small Amazonian forest streams. We sampled fish with hand and seine nets in headwater streams in a 10 000 ha terra‐firme forest reserve near Manaus, Brazil. Each stream was surveyed at the end of the 2005 dry season, at the beginning of the 2006 rainy season and at the beginning of the 2006 dry season, by means of a standardized sampling effort. 3. The numbers of individuals and species caught were higher in the dry season, but rarefaction analyses indicated that greater species numbers could have been due simply to the larger number of individuals caught. 4. Between the dry and rainy season, the direction of changes in species composition in multivariate space varied among sites, especially for quantitative (abundance) data. However, the observed variation among sites was the less than expected if the directions of change were random. 5. Fish assemblages in the second dry season were more similar to those in the previous dry season than expected if changes in species composition among seasons were random. This indicates that a general seasonal pattern in fish assemblages can be detected, despite the existence of some erratic site‐specific changes. 6. Most of the species that showed large seasonal variations in density occupy temporary ponds during the rainy season, when much of the valley is inundated and pond networks form adjacent to streams. Short‐duration lateral migrations to these ponds may play an important role in the seasonal fish‐assemblage dynamics in Amazonian headwater streams. 7. Our results contrast with previous studies on small Amazonian streams, which have found little seasonal change in fish assemblages, and highlight the importance of the abundance of common species as an indicator of general fish assemblage structure in biological monitoring programmes.