Mass Spectrometry-based Absolute Quantification of 20S Proteasome Status for Controlled Ex-vivo Expansion of Human Adipose-derived Mesenchymal Stromal/Stem Cells

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Highlights

• •Design of an MRM assay to determine the absolute quantity and stoichiometry of ubiquitous and tissue-specific human 20S proteasome subtypes.
• •Use of purified isotopically labelled 20S proteasome as internal standard for accurate quantification.
• •Variation in the expression of immunoproteasome in adipocyte-derived stem cells (ADSCs) grown under different O₂ levels might be causal for change in cells differentiation capacity.
• •The status of 20S proteasome during ADSCs expansion might constitute an additional relevant quality control parameter to contribute to predict, among other quality markers, their therapeutic capacity.