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《Molecular & cellular proteomics : MCP》2019,18(2):372-382
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- •Surface loops play an essential role in SH2 domain specificity.
- •Diverse specificities may be obtained from a single SH2 domain by combinatorial mutations in the EF and BG loops.
- •The specificity of a loop mutant correlates with the sequence characteristics of the bait peptide used in its isolation.
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《Molecular & cellular proteomics : MCP》2019,18(6):1157-1170
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- •Auxin responsive proteins in Arabidopsis roots were identified from 3,514 detected proteins.
- •All six auxin receptors are stable in response to hormone via novel MRM assays.
- •The >100 differentially expressed proteins exhibit dynamic and transient responses to auxin.
- •Phenotypic screening of the top responsive proteins uncovered several novel root mutants.
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《Molecular & cellular proteomics : MCP》2019,18(5):936-953
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- •In-depth proteome profiling of primary human myeloma cells
- •Characteristics of myeloma cells are related to hypoxic bone marrow conditions
- •Myeloma cells show specific immune evasion strategies
- •Metabolic adaptations involve tumor and stroma cells
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《Molecular & cellular proteomics : MCP》2019,18(4):622-641
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- •Serial biopsies and autopsy from a metastatic lung cancer patient over 7 years.
- •Tumor heterogeneity characterized by quantifying the proteome and phosphoproteome.
- •Patient-specific database built using whole genome sequencing data from tumors.
- •MRM assay and functional validation of a novel lung-specific CDK12-G879V mutant.
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《Molecular & cellular proteomics : MCP》2018,17(12):2434-2447
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- •Anticancer activity of midostaurin but not other PKC inhibitors in NSCLC cells.
- •Mechanism of action by network-based integration of chemo- and phosphoproteomics.
- •Midostaurin polypharmacology by simultaneous inhibition of TBK1, PDPK1 and AURKA.
- •Design of synergistic drug combination with PLK1 inhibitor by pathway validation.
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《Molecular & cellular proteomics : MCP》2019,18(9):1893-1898
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- •Support for mass spectrometry-based proteomics in cBioPortal.
- •User-friendly web interface, a web API, and an R client to query proteogenomic data.
- •Integration of Clinical Proteomics Tumor Analysis Consortium data with cBioPortal.
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《Molecular & cellular proteomics : MCP》2019,18(10):2029-2043
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- •LFQ-based protein patterns define glioma subgroups that correlate with genomic alterations.
- •Proteomic analysis resolves distinct pathway-level differences among glioma subtypes.
- •Distinct IDH subtype-specific tumor patterns are maintained in glioma stem cell cultures.
9.
《Molecular & cellular proteomics : MCP》2019,18(1):51-64
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- •microRNA-222 attenuates TGEV-induced mitochondrial dysfunction.
- •microRNA-222 downregulates THBS1 and CD47.
- •THBS1 is the target of microRNA-222 during TGEV infection.
- •THBS1 and CD47 increase mitochondrial Ca2+ level and reduced mitochondrial membrane potential (MMP).
10.
《Molecular & cellular proteomics : MCP》2019,18(7):1345-1362
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- •Plant mitoribosomes contain several pentatricopeptide repeat proteins.
- •The small mitoribosomal subunit is of an exceptionally large size.
- •Protein units not directly related to translation may be attached to plant mitoribosomes to confer additional functions to these molecular machines.
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《Molecular & cellular proteomics : MCP》2019,18(2):169-181
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- •Quantitative proteomics of mitotic chromosome scaffold isolated from chicken DT40 cells.
- •BAZ1B identified in the isolated mitotic chromosome scaffold localizes to mitotic chromosome axes.
- •BAZ1B knockout caused prophase delay because of altered chromosome condensation timing and impaired mitosis progression.
- •BAZ1B knockout did not affect prometaphase chromosome structure.
13.
《Molecular & cellular proteomics : MCP》2019,18(2):245-262
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- •AP-DIA/SWATH analysis to identify TCTP-interacting proteins in NF1 tumor cells.
- •A highly specific TCTP–EF1A2 interaction but rather than TCTP–EF1A1 interaction.
- •TCTP–EF1A2 interaction mediating formation of EF1A2-elogation factor complex.
- •TCTP–EF1A2 dependent translation machinery regulating NF1 tumor cell growth.
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《Molecular & cellular proteomics : MCP》2019,18(1):151-161
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- •New MALDI MS imaging sample preparation workflow reveals tissue protease activity.
- •Differential time- and inhibitor concentration-dependence confirm active proteases.
- •Mouse gastric tumor displays high protease activity compared to surrounding tissue.
- •Proteomic data and biochemical protease activity assay support MALDI MSI results.
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《Molecular & cellular proteomics : MCP》2019,18(3):408-422
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- •Quantitative phosphoproteomics of cells treated with sphingolipid analogs or PP2A inhibitor identify novel protein targets of PP2A.
- •PP2A substrates include several nutrient transporter proteins, GTPase regulators and proteins associated with actin cytoskeletal remodeling.
- •Differential regulation of Akt and Gsk3b account for the difference in vacuolating phenotype observed between SH-BC-893 and C2-ceramide.
- •Dynamic phosphoproteomics enabled the correlation of cell signaling with phenotypes to rationalize their mode of action.
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《Molecular & cellular proteomics : MCP》2019,18(9):1796-1806
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- •iTRAQ-based analysis of saliva samples from oral cancer patients.
- •Proteome profiling of saliva samples from patients with oral premalignant lesions.
- •Verification of salivary biomarker candidates with MRM-MS and immunoassays.
- •Identification of salivary proteins as potential biomarkers of oral cancer.
19.
《Molecular & cellular proteomics : MCP》2019,18(4):744-759
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- •Design of an MRM assay to determine the absolute quantity and stoichiometry of ubiquitous and tissue-specific human 20S proteasome subtypes.
- •Use of purified isotopically labelled 20S proteasome as internal standard for accurate quantification.
- •Variation in the expression of immunoproteasome in adipocyte-derived stem cells (ADSCs) grown under different O2 levels might be causal for change in cells differentiation capacity.
- •The status of 20S proteasome during ADSCs expansion might constitute an additional relevant quality control parameter to contribute to predict, among other quality markers, their therapeutic capacity.
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《Molecular & cellular proteomics : MCP》2019,18(9):1782-1795
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- •MEEC are a reliable endocardial in vitro model.
- •Quantitative proteomics to characterize the NOTCH-driven endocardial secretome.
- •NOTCH pathway status underlies different paracrine biological functions.
- •New insights into secreted factors involved in cardiac valve development.