Comprehensive Analysis of Individual Variation in the Urinary Proteome Revealed Significant Gender Differences

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Highlights

• •Comprehensive analysis of inter-individual variation of normal urinary proteome.
• •Significant gender differences were observed.
• •Proteins increased in female urine are enriched in immunological pathways.
• •Estimated reference intervals of proteins as the baseline for biomarker discovery.

     

Agonists of Orally Expressed TRP Channels Stimulate Salivary Secretion and Modify the Salivary Proteome

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Highlights

• •Salivary secretion was increased by mouth rinsing with TRP channel agonists.
• •The salivary proteome varied over time and was changed by TRP channel stimulation.
• •Immunoreactive Cystatin S was increased in saliva after TRPV1 stimulation.

     

Posttranslational Modifications Drive Protein Stability to Control the Dynamic Beer Brewing Proteome

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Highlights

• •SWATH-MS profiles protein abundance and modification during mashing in beer brewing.
• •Proteolysis due to barley proteases leads to extreme proteoform diversity.
• •Sequence-specific proteolytic clipping of proteins controls their stability.

     

Associations of the Fecal Microbial Proteome Composition and Proneness to Diet-induced Obesity

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Highlights

• •Feeding mice a western-style obesogenic diet resulted in dramatic changes in fecal microbial proteome.
• •Basal fecal microbial proteome, but not microbiome, composition was associated with extent of diet-induced obesity.
• •A major feature of fecal microbial proteome of high-responder mice was enriched motility-related proteins.

     

A Combined N-terminomics and Shotgun Proteomics Approach to Investigate the Responses of Human Cells to Rapamycin and Zinc at the Mitochondrial Level

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Highlights

• •Rapamycin and zinc induce moderate but significant mitochondrial proteome changes.
• •The mitochondrial proteins processing system is robust under subtoxic conditions.
• •Rapamycin and zinc perturb the mitochondrial proteins processing system.
• •Rapamycin and zinc perturb the mitochondrial proteins homeostasis.

     

耐碳青霉烯类肺炎克雷伯菌的耐药机制研究

目的了解碳青霉烯类耐药肺炎克雷伯菌及耐药机制。方法对2012-2013年临床分离的耐碳青霉烯类肺炎克雷伯菌共计12株进行分析,药敏采用MIC方法检测,用WHONET 5.6软件进行分析,KPC表型检测采用改良Hodge试验,基因检测采用PCR方法。结果 12株碳青霉烯类耐药肺炎克雷伯菌改良Hodge试验阴性,基因测序为KPC-2型。结论 KPC-2基因是引起本院肺炎克雷伯菌耐药的主要原因。     

耐碳青霉烯类肺炎克雷伯菌血流感染危险因素及预后分析

目的 探讨碳青霉烯类耐药肺炎克雷伯菌血流感染（CSKP）的危险因素以及影响患者28 d预后的相关因素。方法 回顾性分析我院2016年1月至2017年12月期间住院的肺炎克雷伯菌血流感染患者的临床病史资料,按患者血培养标本采集后28 d内预后情况分为存活组与死亡组,应用单因素分析及多因素Logistic回归分析探讨碳青霉烯耐药肺炎克雷伯菌血流感染的危险因素,应用Cox回归分析研究影响肺炎克雷伯菌血流感染28 d预后的相关因素。结果 耐碳青霉烯类肺炎克雷伯菌血流感染的危险因素包括高APACHEⅡ评分、高Pitt菌血症评分、感染时入住ICU、感染前30 d内手术、有创操作、深静脉置管、有创机械通气、器官移植、使用免疫抑制剂、感染前3个月内入住ICU和感染前使用抗菌药物。Logistic回归分析显示高APACHEⅡ评分（OR=1.066,95% CI：1.027~1.107,P=0.001）、手术（OR=3.777,95% CI：1.816~7.855,P<0.001）、有创操作（OR=2.864,95% CI：1.303~6.295,P=0.009）、器官移植（OR=3.892,95% CI：1.553~9.752,P=0.004）、感染前使用抗菌药物（OR=5.626,95% CI：2.740~11.553,P<0.001）是发生碳青霉烯类耐药的肺炎克雷伯菌血流感染的独立危险因素。影响肺炎克雷伯菌血流感染28 d预后的相关因素有高APACHEⅡ评分、高Pitt菌血症评分、感染时入住ICU、感染前30 d内手术、有创操作、深静脉置管、有创机械通气、器官移植、感染前3个月内ICU入住史、使用抗菌药物、粒细胞缺乏、血液透析和菌株对碳青霉烯类耐药。Cox回归分析发现高APACHEⅡ评分（HR=1.061,95% CI：1.039~1.084,P<0.001）、有创操作（HR=2.505,95% CI：1.239~5.063,P=0.011）、入住ICU（HR=1.589,95% CI：1.042~2.424,P=0.031）是影响患者预后的独立危险因素。耐碳青霉烯类肺炎克雷伯菌（CRKP）血流感染患者的28 d病死率明显高于碳青霉烯类敏感肺炎克雷伯菌感染患者（χ2=41.612,P<0.001）。结论 高APACHEⅡ评分、手术、有创操作、器官移植、感染前使用抗菌药物可导致耐碳青霉烯类肺炎克雷伯菌血流感染的发生风险增加。CRKP血流感染患者死亡率显著高于CSKP感染者,但CRKP感染并非患者短期死亡的独立危险因素。而高APACHEⅡ评分、有创操作、入住ICU则可显著增加患者短期病死率。     

Exploring Regulation of Protein O-Glycosylation in Isogenic Human HEK293 Cells by Differential O-Glycoproteomics

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Highlights

• •A panel of HEK293 isogenic cell lines with knockout of GALNT genes.
• •Identification of nonredundant O-glycosylation sites regulated by specific GalNAc-T isoforms.
• •GalNAc-T7 and T10 contribute to follow-up activity in regions of high density O-glycosylation.
• •GalNAc-T11 specifically controls O-glycosylation of specific linker regions in the low-density lipoprotein receptor related proteins.

     

肺炎克雷伯菌临床分离株的庆大霉素耐药性和细菌铁载体的作用

目的 研究临床分离的肺炎克雷伯菌对氨基糖苷类抗生素庆大霉素的耐药性与其产铁载体的关系。方法 采用K-B纸片法和肉汤稀释法确定70株临床分离的肺炎克雷伯菌对庆大霉素的药物敏感性;CAS琼脂实验检测肺炎克雷伯菌是否产铁载体;紫外可见分光光度法确定细菌产铁载体的量,根据中位数法将70株临床分离菌分为铁载体高产组（35株）和低产组（35株）;应用SPSS统计学软件分析抗生素耐药性与其产铁载体是否相关。结果 药物敏感性试验检测出菌株对庆大霉素的耐药率为50.00%（35/70）;铁载体检测实验确定70株肺炎克雷伯菌均产生铁载体,肺炎克雷伯菌对庆大霉素的耐药性与铁载体产量呈正相关关系（r=0.3154,P<0.05）,对庆大霉素耐药菌株铁载体产量明显高于敏感菌株（t=3.1650,P<0.05）,且铁载体高产组耐药率及lgMIC值明显高于低产组（χ2=9.6570,t=3.1360,P<0.05）。结论 70株临床分离的肺炎克雷伯菌均产生铁载体,铁载体可能参与肺炎克雷伯菌对庆大霉素的耐药,干扰庆大霉素的抑菌或杀菌过程。     

Simultaneous Quantification of Protein Expression and Modifications by Top-down Targeted Proteomics: A Case of the Sarcomeric Subproteome

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Highlights

• •A new strategy for simultaneous quantification of protein expression and modification.
• •This top-down LC/MS-based method shows high reproducibility and high throughput.
• •Quantification at the intact protein level with results comparable to Western blot.
• •This top-down proteomics method is applicable to different species and tissues.

     

Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation*

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Highlights

• •Characterization of the phagosomal proteome comparing resting and LPS-treated BMDCs.
• •Label-free quantification determined 2843 phagosomal proteins.
• •Reduced recruitment of hydrolases and V-ATPase to phagosomes of LPS-treated cells.
• •Increased recruitment of antigen cross-presentation molecules to these phagosomes.

     

临床分离肺炎克雷伯菌耐药性监测

目的了解临床分离肺炎克雷伯菌的耐药性,为临床合理应用抗菌药物提供实验室依据。方法采用微量稀释法对392例临床分离肺炎克雷伯菌进行药物敏感性测定;超广谱β-内酰胺酶(Extendedspectrumbeta-lactamases,ESBLs)检测用微量稀释法初筛,纸片法做确证试验。结果肺炎克雷伯菌对18种抗菌药物的药敏结果中,耐药率大于30%的抗菌药物多达11种;其中氨苄西林-舒巴坦、氨苄西林、头孢噻吩、哌拉西林、复方新诺明和头孢唑啉的耐药率高达20%以上。耐药率低于10%的抗菌药物仅有4种,分别为头孢曲松(7.7%)、头孢噻肟(7.4%)、氨曲南(6.9%)和亚胺培南(3.1%)。其它抗菌药物的耐药率都高于10%。产ESBLs菌株的发生率为32.9%～45.8%,平均为39.8%;产ESBLs菌株对多种抗菌药物的耐药率显著高于非产ESBLs菌株(P<0.05)。结论临床分离肺炎克雷伯菌对多种抗菌药物的耐药率较高,尤其是产ESBLs菌株的高耐药率及多重耐药性更为明显。临床应加强对肺炎克雷伯菌耐药性的监测并预防耐药菌株的传播流行。