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1.
《Molecular & cellular proteomics : MCP》2019,18(10):2108-2120
Highlights
- •Bayesian Beta-Binomial model integrates ion statistics with peptide ratio agreement.
- •Model appropriately interprets information from low signal peptides.
- •Confidence can be assigned even without replicates.
- •Model adds sensitivity to detection of small changes.
2.
《Molecular & cellular proteomics : MCP》2019,18(5):936-953
Highlights
- •In-depth proteome profiling of primary human myeloma cells
- •Characteristics of myeloma cells are related to hypoxic bone marrow conditions
- •Myeloma cells show specific immune evasion strategies
- •Metabolic adaptations involve tumor and stroma cells
3.
《Molecular & cellular proteomics : MCP》2019,18(2):383-390
Highlights
- •Fast and simple capillary column packing protocol.
- •Low-pressure packing at <100 bars from ultrahigh sorbent suspension concentration.
- •Sorbent particle aggregation leading to blocking of the column entrance is avoided.
- •Effective for long capillary UHPLC column packing with a wide range of sorbents.
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5.
《Molecular & cellular proteomics : MCP》2019,18(8):1619-1629
Highlights
- •Quantitative proteomics analysis of cancer cell derived small extracellular vesicles (sEVs) reveals metastasis related proteins.
- •HGF/c-Met signaling pathway is mainly activated by cancer cell-secreted sEVs-HGF.
- •sEVs-HGF plays essential role in the metastasis of cancer cells.
6.
《Molecular & cellular proteomics : MCP》2019,18(1):151-161
Highlights
- •New MALDI MS imaging sample preparation workflow reveals tissue protease activity.
- •Differential time- and inhibitor concentration-dependence confirm active proteases.
- •Mouse gastric tumor displays high protease activity compared to surrounding tissue.
- •Proteomic data and biochemical protease activity assay support MALDI MSI results.
7.
《Molecular & cellular proteomics : MCP》2018,17(11):2146-2163
Highlights
- •Construction of threespine stickleback gill assay library using DDA proteomics
- •Population-specific gill proteome signatures of four ecotypes identified by DIA
- •HSP47 and extracellular matrix proteins highly elevated in warm-adapted sticklebacks
- •Inflammasome and proteolytic proteins highly elevated in freshwater sticklebacks
8.
《Molecular & cellular proteomics : MCP》2019,18(2):200-215
Highlights
- •A global lysine succinylome was investigated in A. hydrophila.
- •The lysine succinylation modifications play crucial role on various metabolic pathways.
- •Reversible succinylation on Lys23 and Lys30 regulates the activity of S-ribosylhomocysteine lyase LuxS.
- •Lysine succinylation modifications of LuxS affect quorum sensing and metabolism.
9.
《Molecular & cellular proteomics : MCP》2019,18(3):594-605
Highlights
- •A new strategy for simultaneous quantification of protein expression and modification.
- •This top-down LC/MS-based method shows high reproducibility and high throughput.
- •Quantification at the intact protein level with results comparable to Western blot.
- •This top-down proteomics method is applicable to different species and tissues.
10.
《Molecular & cellular proteomics : MCP》2019,18(7):1396-1409
Highlights
- •A panel of HEK293 isogenic cell lines with knockout of GALNT genes.
- •Identification of nonredundant O-glycosylation sites regulated by specific GalNAc-T isoforms.
- •GalNAc-T7 and T10 contribute to follow-up activity in regions of high density O-glycosylation.
- •GalNAc-T11 specifically controls O-glycosylation of specific linker regions in the low-density lipoprotein receptor related proteins.
11.
12.
《Molecular & cellular proteomics : MCP》2019,18(6):1183-1196
Highlights
- •Atlantic salmon O-glycome expanded to 169 structures in three epithelia.
- •Low interindividual variation amongst all populations and geographical regions.
- •Small variations in glycosylation between geographical locations and fish size.
- •Prominent fucosylation in gastrointestinal mucins from Tasmanian fish.
13.
《Molecular & cellular proteomics : MCP》2019,18(6):1085-1095
Highlights
- •Rapamycin and zinc induce moderate but significant mitochondrial proteome changes.
- •The mitochondrial proteins processing system is robust under subtoxic conditions.
- •Rapamycin and zinc perturb the mitochondrial proteins processing system.
- •Rapamycin and zinc perturb the mitochondrial proteins homeostasis.
14.
《Molecular & cellular proteomics : MCP》2019,18(3):490-503
Highlights
- •MHC-II-bound peptide repertoires from DO-sufficient and DO-deficient cells.
- •Fewer unique peptides and core epitopes were presented in the absence of DO.
- •Immunopeptidome differences appeared to result from reduced DM editing.
- •DO-dependent self-epitopes elicited CD4 T cell responses in mice.
15.
《Molecular & cellular proteomics : MCP》2019,18(1):41-50
Highlights
- •Proteome of mature boar spermatozoa from cauda epididymal and ejaculated sources were analyzed by iTRAQ-based LC-MS/MS.
- •1,723 sperm proteins identified (974 of Sus scrofa taxonomy).
- •1,602 sperm proteins quantified (960 of Sus scrofa taxonomy).
- •32 Sus scrofa sperm proteins were differentially expressed among sperm sources.
- •The proteome of boar spermatozoa is remodelled during ejaculation.
16.
《Molecular & cellular proteomics : MCP》2018,17(11):2119-2131
Highlights
- •Temporal proteome profiling of lipotoxicity and glucolipotoxicity in β-cells
- •Palmitate induced cholesterol metabolism earlier than fatty acid metabolism
- •Setd8 promotes palmitate + glucose-stimulated INS-1 cell proliferation
- •PA induced apoptosis partially via upregulation of Rhob in INS-1 cells
17.
《Molecular & cellular proteomics : MCP》2019,18(10):2029-2043
Highlights
- •LFQ-based protein patterns define glioma subgroups that correlate with genomic alterations.
- •Proteomic analysis resolves distinct pathway-level differences among glioma subtypes.
- •Distinct IDH subtype-specific tumor patterns are maintained in glioma stem cell cultures.
18.
《Molecular & cellular proteomics : MCP》2019,18(3):520-533
Highlights
- •1. Celastrol alleviated cholestatic liver injury.
- •2. Celastrol inhibited the decrease of SIRT1 induced by deoxycholic acid.
- •3. SIRT1-FXR signaling pathway mediated the effect of celastrol.
19.
《Molecular & cellular proteomics : MCP》2019,18(6):1110-1122
Highlights
- •Comprehensive analysis of inter-individual variation of normal urinary proteome.
- •Significant gender differences were observed.
- •Proteins increased in female urine are enriched in immunological pathways.
- •Estimated reference intervals of proteins as the baseline for biomarker discovery.
20.
《Molecular & cellular proteomics : MCP》2019,18(4):657-668
Highlights
- •Global proteomic remodeling alters antibiotic susceptibility in K. pneumoniae.
- •Drug specific transport, sugar utilization, central metabolism, capsule synthesis.
- •Common pathways of reactive oxygen scavenging, turnover of misfolded proteins.
- •Integrated adjustments and unique drug-specific features for drug combinations.