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《Molecular & cellular proteomics : MCP》2019,18(6):1123-1137
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- •Changes to the proteome of skin fibroblasts subjected to reductive stress have been quantitated.
- •Only a small set of proteins is selectively diminished upon exposure to reductants.
- •Collagens (COL1A2 and COL6A2) emerge as sentinels of reductive stress.
- •Reductive stress triggers receptor-independent Akt phosphorylation at Ser473.
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《Molecular & cellular proteomics : MCP》2019,18(9):1880-1892
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- •New software to determine differentially expressed proteins in quantitative MS experiments.
- •Applicable to any modern quantitative MS setup and study type, including clinical setups.
- •Ultra-sensitive at strict FDR control, up to 1000 additional proteins in a single comparison.
- •Easy to use, fast processing and readily available package, including user friendly manual.
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《Molecular & cellular proteomics : MCP》2019,18(3):546-560
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- •Proteomic analysis of cerebrospinal fluid and identification of synaptic component.
- •Use of super resolution microscopy to verify synapse-specificity in human tissue.
- •Selective reaction monitoring MS (SRM) of synaptic panel in 3 cohorts of Alzheimer's disease cerebrospinal fluid.
- •Synaptic protein changes precede tau in preclinical Alzheimer's disease.
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《Molecular & cellular proteomics : MCP》2019,18(6):1085-1095
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- •Rapamycin and zinc induce moderate but significant mitochondrial proteome changes.
- •The mitochondrial proteins processing system is robust under subtoxic conditions.
- •Rapamycin and zinc perturb the mitochondrial proteins processing system.
- •Rapamycin and zinc perturb the mitochondrial proteins homeostasis.
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《Molecular & cellular proteomics : MCP》2018,17(11):2146-2163
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- •Construction of threespine stickleback gill assay library using DDA proteomics
- •Population-specific gill proteome signatures of four ecotypes identified by DIA
- •HSP47 and extracellular matrix proteins highly elevated in warm-adapted sticklebacks
- •Inflammasome and proteolytic proteins highly elevated in freshwater sticklebacks
6.
《Molecular & cellular proteomics : MCP》2019,18(9):1807-1823
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- •Seventy-six most promising proteins were qualified, and 19 proteins were verified by SRM in 219 seminal plasma samples of patients with prostate cancer and negative biopsies.
- •Prostate-specific, secreted and androgen-regulated protein-glutamine gamma-glutamyltransferase 4 (TGM4) was verified by SRM assay and an in-house immunoassay.
- •TGM4 detected prostate cancer on biopsy in seminal plasma (AUC=0.66), but not in blood serum.
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《Molecular & cellular proteomics : MCP》2019,18(12):2359-2372
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- •Nonenzymatically Ksu proteins shown different pattern from native cell Ksu proteins.
- •Motif preference of Ksu proteins was associated with different biological processes.
- •Up to 67 developing rice seeds proteins contain PTMs of Kac, Ksu, Kcr, Kmal, and Khib.
- •Some lysine residues of the key pathway enzymes are modified by succinylation.
9.
《Molecular & cellular proteomics : MCP》2019,18(9):1732-1744
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- •An innovative co-IP crosslinking proteomics study was designed for the TLR2 interactome.
- •Proteomic profiling revealed combinatorial effects of simvastatin and Pam3CSK4 on the TLR2 interactome.
- •ACTR1A and MARCKSL1 proteins were identified as potential interactors of TLR2 during the immune response.
- •ACTR1A has important modulatory actions on the TLR2 pro-inflammatory signaling cascade.
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Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation*
《Molecular & cellular proteomics : MCP》2019,18(5):909-922
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- •Characterization of the phagosomal proteome comparing resting and LPS-treated BMDCs.
- •Label-free quantification determined 2843 phagosomal proteins.
- •Reduced recruitment of hydrolases and V-ATPase to phagosomes of LPS-treated cells.
- •Increased recruitment of antigen cross-presentation molecules to these phagosomes.
11.
《Molecular & cellular proteomics : MCP》2018,17(11):2242-2255
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- •BioID and IP-MS were conducted to generate a global ZIKV-host protein interactome
- •Interactome consists of >3000 high confidence ZIKV-host protein interactions
- •Data mining indicates that ZIKV proteins interact with multiple host cell organelles
- •An important role for peroxisomes in ZIKV infection is uncovered
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《Molecular & cellular proteomics : MCP》2018,17(12):2371-2386
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- •Stability of oxidative phosphorylation subunits are reduced in a diet-induced mouse model of NAFLD.
- •These changes are associated with impaired activities of electron transport chain complexes and ATP synthesis.
- •Increased mitophagy contributed to enhanced degradation of mitochondrial proteins.
15.
《Molecular & cellular proteomics : MCP》2019,18(6):1110-1122
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- •Comprehensive analysis of inter-individual variation of normal urinary proteome.
- •Significant gender differences were observed.
- •Proteins increased in female urine are enriched in immunological pathways.
- •Estimated reference intervals of proteins as the baseline for biomarker discovery.
16.
《Molecular & cellular proteomics : MCP》2019,18(12):2506-2515
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- •Method for the analysis of response curves from thermal proteome profiling (TPP).
- •NPARC uses nonparametric statistics and provides false discovery-rate (FDR) control.
- •Increased proteome coverage and sensitivity to identify drug-binding proteins.
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《Molecular & cellular proteomics : MCP》2019,18(3):408-422
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- •Quantitative phosphoproteomics of cells treated with sphingolipid analogs or PP2A inhibitor identify novel protein targets of PP2A.
- •PP2A substrates include several nutrient transporter proteins, GTPase regulators and proteins associated with actin cytoskeletal remodeling.
- •Differential regulation of Akt and Gsk3b account for the difference in vacuolating phenotype observed between SH-BC-893 and C2-ceramide.
- •Dynamic phosphoproteomics enabled the correlation of cell signaling with phenotypes to rationalize their mode of action.
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《Molecular & cellular proteomics : MCP》2019,18(11):2244-2261
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- •Proteomic analysis of brain and cerebrospinal fluid from mouse NCL models.
- •Disease-associated changes in lysosomal proteins and markers of neuroinflammation.
- •Biomarker candidates identified for further evaluation.
19.
《Molecular & cellular proteomics : MCP》2019,18(1):28-40
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- •Method to probe the isomeric variants of the glycans attached to purified proteins.
- •Uses multiple rounds of glycosidase cleavage and lectin profiling.
- •Computation integration of lectin-binding, glycan-array, and mass spectrometry data.
- •Applied to microspots for compatibility with analyzing low-abundance proteins.
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《Molecular & cellular proteomics : MCP》2019,18(3):461-476
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- •Genital samples were analyzed using a high-throughput bead-based affinity assay.
- •Proteins were validated by tandem mass spectrometry with concordant results.
- •Genital epithelial proteins were increased in HIV-serodiscordant women.