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1.
Evidence for the Colonization of Lactic Acid Bacteria in the Gastrointestinal Tract of Suckling Mink
Lactic acid bacteria are considered indigenous members of the gastrointestinal microflora in a number of animal species (Savage 1977a). Some intestinal strains of lactobacilli and streptococci are aWe to adhere to stratified squamous epithelium of some animals (Tannock et al. 1987), in the non-secreting part of the stomach of piglets (Barrow et al. 1980, Fuller et al. 1978) and rodents (Tannock et al. 1982), and in the crop of poultry (Fuller 1978). The presence of lactic acid bacteria in the digestive tract is believed to be of beneficial value to the host animal (Fuller 1989). The production of organic acids in the stomach or the crop helps maintaining a low pH which may be important for inhibiting the colonization of potentially pathogenic bacteria, particularly in the newborn animal (Barrow et al 1980, Fuller 1977, Fuller 1978). The adhesion of lactobacilli to squamous epithelium is host specific: strains capable of adhering to the epithelium of piglets are usually not able to adhere in rodents or poultry and vice versa (Fuller 1978, Lin & Savage 1984, Tannock et al 1982). Adhesion of lactic acid bacterial strains to other epithelia than stratified squamous epithelium has been reported. Thus, the attachment of lactobacilli to cells from the secreting epithelium of the murine stomach (Kotarski & Savage 1979), to intestinal cells of humans (Goldin & Gorbach 1987), and to columnar epithelial cells of piglets and calves (Mäyrä-Mäkinen et al 1983) has been demonstrated using in vitro methods. In another study the in vivo attachment of Enterococcus faecium to duodenal epithelium of gnotobi-otic chickens was demonstrated (Fuller et al 1981). Recent research indicated that in adult mink lactic acid bacteria are not indigenous members of the intestinal flora, and they do not attach to epithelium in any part of the gastrointestinal tract (Federsen & Jørgensen 1992). The present paper presents evidence that Gram positive cocci may colonize the gut of suckling mink kits and attach to the gut mucosa. 相似文献
2.
Tissue expression of three structurally different fatty acid binding proteins from rat heart muscle, liver, and intestine 总被引:4,自引:0,他引:4
Three structurally different 14-15 kDa fatty acid binding proteins have have been purified from rat liver, small intestinal epithelium, and heart muscle, and were quantitated using specific antisera in rat tissues. Heart muscle fatty acid binding protein comprised 5% of heart muscle cytosol protein and was also expressed in stomach, muscle, testis, ovary, kidney, brain, and adipose tissue, a pattern distinct from both liver protein (expressed in liver, small and large intestinal epithelium, and adipose tissue) and intestinal protein (expressed in small and large intestinal epithelium and stomach). Distinctive patterns of tissue expression of the three different fatty acid binding proteins suggest that they may perform different specific functions in fatty acid transport and metabolism. 相似文献
3.
Peroxisomes, participants in lipid metabolism, have been shown to be altered in liver in two metabolic diseases in which long-chain fatty acids accumulate in tissues: Zellweger's syndrome and neonatal adrenoleukodystrophy (ALD). The intestine also plays a role in lipid metabolism, and we have had the opportunity to compare peroxisomes in normal intestinal epithelium with those from patients with Zellweger's syndrome and neonatal ALD at the electron microscopic level by using the combined techniques of cytochemistry and stereological analysis. Peroxisomes were numerous in intestinal epithelium of the normal individuals. They were ellipsoidal in shape with average diameters of 0.37 by 0.56 micron and filled with coarsely granular, DAB+ content. Peroxisomes in the intestinal epithelium of the ALD patient were similar in appearance and number but smaller in size (0.28 by 0.44 micron). Peroxisomes of normal appearance were absent from the intestinal epithelium of patients with Zellweger's syndrome; DAB+ content, however, was observed in rare, membrane-bound structures of much smaller size (0.12 by 0.19 micron). In liver of patients with Zellweger's syndrome, peroxisomes are lacking; in neonatal ALD they are abnormal in appearance and greatly reduced in number. The presence of rare minute peroxisomes in the intestinal epithelium in Zellweger's syndrome and of small peroxisomes in this epithelium in neonatal ALD indicate that peroxisomes in the intestinal epithelium are affected in these diseases, but to a lesser extent than in the liver. In the ALD intestinal epithelium, DAB+ material was also seen in long, sinuous, tubular or cisternal elements intermingled and occasionally in continuity with peroxisomes. It is suggested that these represent the early stages of peroxisome formation, the peroxisomal reticulum as originally envisioned by Lazarow, while the rare structures seen in Zellweger's represent rudiments of such a reticulum. Lamellar inclusions and clear spaces occurred in the cytoplasm adjacent to these structures indicating either that material accumulated there had been extracted during fixation or that these regions are more susceptible to autolysis. Mitochondria are also involved in lipid metabolism and have been reported to be abnormal in Zellweger's tissue. No qualitative differences were observed in the mitochondria of the intestinal epithelia examined in this study. Although quantitation revealed a greater mean volume, number, and surface density of mitochondria in the intestinal epithelia of neonatal ALD, it was not a statistically significant difference in all cases. 相似文献
4.
Ulla Domar Berith Nilsson Vladimir Baranov Ulf Gerdes Torgny Stigbrand 《Histochemistry and cell biology》1992,98(6):359-364
Human intestinal alkaline phosphatase was immunohistochemically identified and localized in the pancreas, liver and kidney by use of a monoclonal antibody specific for intestinal alkaline phosphatase isozyme and by amplified biotin-streptavidin staining. In all the examined organs, the intestinal isozyme was found to be localized in the epithelial cells of ducts: bile ducts in the liver, distal convoluted tubules and collecting tubules in the kidney and ducts in the secretory epithelium in the pancreas. In the liver the antibody also stained some sinus-lining cells. In all the examined organs the endothelial cells of the capillaries and some vessels were stained. By use of immunoelectron microscopy, intestinal alkaline phosphatase was, as expected, found to be localized to the microvillar region of the small intestine. The isozyme was abundantly expressed in the apical area of the microvilli and in membrane remnants in the fuzzy coat. Capillaries and vessels in the submucosa were also stained, as well as small vesicles in the endothelial cells. The present investigation demonstrates the expression and localization of the intestinal alkaline phosphatase in several organs, though previously believed to be expressed only in the intestine. 相似文献
5.
Twelve 2–5-week-old calves affected with a spontaneous intestinal disorder were examined; 8 had diarrhea and 4 were convalescents. In all the affected calves the “pseudovilli” (syn. domes or lymphoid villi) over Peyer’s patches seemed atrophic and appeared enclosed within the mucosa, owing to fusion of ordinary villi with “pseudovilli”. Morphometric examination showed a decrease of lymphoid follicle length in the affected calves as compared with controls (P < 0.01). Convalescents showed longer follicles than diarrheic calves (P<0.05). Often cytoplasmic acid phosphatase of the follicle-associated epithelium (FAE) in affected calves did not show the marked basal-apical decrease along “pseudovillus”, typical of the controls. Scanning electron microscopy revealed sparse development of concentric folds in the luminal plasma membrane of the enclosed FAE, contrasting with their abundance in the normal FAE. Transmission electron microscopy showed that the “pseudovilli” had increased numbers of ordinary villous epithelial cells. Affinity of chlamydia for FAE was shown. It is suggested that the sparse occurrence of surface folds in the FAE and the change in acid phosphatase distribution indicate diminished endocytosis of antigenic material, probably resulting from the enclosure of “pseudovilli”. The atrophy of lymphoid follicles may be another expression of the probable decreased contact with the intestinal contents. 相似文献
6.
Expression of intestinal alkaline phosphatase in human organs. 总被引:1,自引:0,他引:1
Human intestinal alkaline phosphatase was immunohistochemically identified and localized in the pancreas, liver and kidney by use of a monoclonal antibody specific for intestinal alkaline phosphatase isozyme and by amplified biotin-streptavidin staining. In all the examined organs, the intestinal isozyme was found to be localized in the epithelial cells of ducts: bile ducts in the liver, distal convoluted tubules and collecting tubules in the kidney and ducts in the secretory epithelium in the pancreas. In the liver the antibody also stained some sinus-lining cells. In all the examined organs the endothelial cells of the capillaries and some vessels were stained. By use of immunoelectron microscopy, intestinal alkaline phosphatase was, as expected, found to be localized to the microvillar region of the small intestine. The isozyme was abundantly expressed in the apical area of the microvilli and in membrane remnants in the fuzzy coat. Capillaries and vessels in the submucosa were also stained, as well as small vesicles in the endothelial cells. The present investigation demonstrates the expression and localization of the intestinal alkaline phosphatase in several organs, though previously believed to be expressed only in the intestine. 相似文献
7.
L M Isachkova A A Zhavoronkov N F Timchenko 《Biulleten' eksperimental'no? biologii i meditsiny》1985,100(7):117-120
Some data showing specific ways of interaction of Yersinia pseudotuberculosis with the epithelium of the small intestinal mucosa have been received for the first time by using light microscopy of semithin sections. Comparative study of the lesion of the epithelium of different parts of rabbit small intestine by oral inoculation with Yersinia tuberculosis has expanded the ideas of the infection entry. It has been established for the first time that the most intensive penetration of the microorganisms into the epithelium of the mucous membrane takes place in the duodenum and jejunum. As the infected contents is evacuated via the intestinal tract, the Yersinia pathogenicity decreases, and in the ileum, the invasion of the microorganisms becomes less pronounced. It has been revealed that in pseudotuberculosis, there takes place a repeated dissemination of the infection in the intestinal tract because of decay of the involved enterocytes, which is of pathogenetic importance for the progress and outcome of the infection. 相似文献
8.
Gert H. Hansen Karina Rasmussen Lise-Lotte Niels-Christiansen E. Michael Danielsen 《Histochemistry and cell biology》2009,131(6):727-732
Lipopolysaccharide (LPS)-binding protein (LBP) is an acute-phase protein involved in the host’s response to endotoxin and
mainly synthesized and secreted to the blood by the liver. But in addition, LBP is also made by extrahepatic cells, including
the enterocyte-like cell line Caco-2. To study in closer detail the synthesis and storage of LBP in the intestinal mucosal
epithelium, we performed an immunolocalization of LBP in mouse small intestine. By immunofluorescence microscopy, an antibody
recognizing the 58–60 kDa protein of LBP distinctly labeled a small population of cells located deep into the crypts. This
cell population was also positive for lysozyme and α-defensin 4, identifying Paneth cells as the main intestinal LBP-producing
cells. By immunogold electron microscopy, intense labeling was observed in the secretory granules of these cells. We conclude
that Paneth cells express LBP together with other proteins acting in the innate immune response of the gut, such as lysozyme,
defensins and intelectin. 相似文献
9.
Kohtaro Asayama Kazushige Dobashi Yasusuke Kawada Takaya Nakane Akira Kawaoi Shinpei Nakazawa 《The Histochemical journal》1996,28(1):63-71
Summary To quantitate the developmental changes in selenium-dependent cellular glutathione peroxidase during the perinatal period,
tissue sections from foetal (day 12 to day 22) and neonatal (day 6) rats were stained immunohistochemically using specific
polyclonal antiserum. The intensity of the staining was quantified by fluorescence microscopy image analysis. There was a
general trend of enriched glutathione peroxidase in the epithelial linings and metabolically active sites. Significant fluorescence
was detected in cardiomyocytes, hepatocytes, renal tubular epithelium, bronchiolar epithelium and intestinal epithelium at
day 15. The intensity increased in a stepwise manner therafter. The overall increase in the intensity of staining in the heart,
liver, kidneys, lungs and intestine was 1.5-, 2.3-, 1.6-, 1.7- and 3.0-fold, respectively. The phase of most rapid increase
occurred during the foetal period in the liver, intestine and heart. In the kidneys and lungs, glutathione peroxidase increased
significantly during foetal life, and to a similar extent postnatally. These results suggest that the intracellular H2O2-scavenging system develops during the foetal period as an essential mechanism for living under atmospheric oxygen conditions.
The late development observed in the kidneys and lungs is consistent with the relative biological immaturity of these organs
in full-term neonates. 相似文献
10.
M L Hermiston C B Latham J I Gordon K A Roth 《The journal of histochemistry and cytochemistry》1992,40(9):1283-1290
To study the geographic differentiation of the intestinal epithelium and to understand the complex lineage relationships of its cell populations, it is often necessary to visualize the protein products of multiple genes in sections prepared from different positions along the duodenal-to-colonic and/or crypt-to-villus axes. Multilabel fluorescence or brightfield immunohistochemical techniques have previously been used for this purpose. However, the number of antigens that can be identified on single sections is limited in fluorescence microscopy by the number of fluorophores with non-overlapping absorption and emission characteristics, in brightfield microscopy by the number of visually distinguishable chromogens, and in both methods by the availability of primary antisera raised in multiple species. We have now used a combination of light and fluorescence microscopic techniques to increase the number of antigens that can be detected in a single section to six. Sections were sequentially stained using immunogold with silver intensification, peroxidase-antiperoxidase with diaminobenzidine chromogen, and peroxidase-anti-peroxidase with alpha-naphthol/basic dye as chromogen, followed by simultaneous fluorescent detection with fluorescein, 7-amino-4-methylcoumarin-3-acetic acid, and beta-phycoerythrin. This method enables up to four separate antigens to be visualized within a single cell and two additional antigens to be detected in unrelated cells. The technique is illustrated by examining the cellular patterns of expression of liver fatty acid binding protein/human growth hormone fusion genes in the intestinal epithelium of adult transgenic mice. It should be generally applicable to other experimental systems that require localization of multiple antigens in single tissue sections. 相似文献
11.
In recent years scanning electron microscopy has been used in gastric biopsy studies, contributing to better recognition of intestinal metaplasia and carcinoma, as a complement to light and transmission electron microscopy. During the second half of 1983, 53 cases of gastric carcinoma were diagnosed at the Department of Pathology of Hospital Mexico, of which six were studied ultrastructurally. A pattern similar to that of intestinal epithelium was found in cases of intestinal metaplasia. Well differentiated adenocarcinomas showed marked tumor cell proliferation with irregular "projections". In poorly differentiated carcinomas, changes were limited to areas where tumor cells invaded the epithelial surface. In summary, scanning electron microscopy is of great help in research and diagnosis of pathologic changes occurring in mucosal surfaces. 相似文献
12.
Sakaguchi T Gu X Golden HM Suh E Rhoads DB Reinecker HC 《The Journal of biological chemistry》2002,277(24):21361-21370
13.
Alain D. Silk Charles E. Gast Paige S. Davies Farnaz D. Fakhari Gretchen E. Vanderbeek Motomi Mori Melissa H. Wong 《PloS one》2013,8(1)
Following transplantation of hematopoietic lineage cells, genetic markers unique to the transplanted cells have been detected in non-hematopoietic recipient cells of human liver, vascular endothelium, intestinal epithelium and brain. The underlying mechanisms by which this occurs are unclear. Evidence from mice suggests it is due in part to fusion between cells of hematopoietic and non-hematopoietic origins; however, direct evidence for this in humans is scant. Here, by quantitative and statistical analysis of X- and Y-chromosome numbers in epithelial and non-epithelial intestinal cells from gender-mismatched hematopoietic cell transplant patients, we provide evidence that transplanted cells of the hematopoietic lineage incorporate into human intestinal epithelium through cell fusion. This is the first definitive identification of cell fusion between hematopoietic cells and any epithelial cell type in humans, and provides the basis for further understanding the physiological and potential pathological consequences of cell fusion in humans. 相似文献
14.
研究了成年雌性黑麂(Muntiacus crinifrons)的肝、小肠和大肠的组织学结构及Ghrelin的分布。采用H.E染色法观察组织学结构,免疫组化PV-9000两步法并结合DAB显色技术确定Ghrelin的分布。结果表明,黑麂的肝组织分为被膜、肝小叶、肝中央静脉、门管区等结构。被膜为浆膜结构,肝小叶不明显。肝细胞以中央静脉为中心,呈放射状排列。肝血窦的形状不规则。肠黏膜上皮为单层柱状上皮,小肠、盲肠和结肠的黏膜肌层很薄,管壁皱襞与肠绒毛等形态在消化道各部也存在差异。免疫组化结果显示肝细胞中有Ghrelin阳性细胞的表达;在肠道,免疫阳性细胞在十二指肠、空肠、回肠、盲肠和直肠的黏膜、黏膜下层和肌层均有分布,尤其在肠绒毛上皮和黏膜下层分布较多。黑麂肝、小肠和大肠结构与哺乳动物基本相似,但无十二指肠腺;Ghrelin阳性细胞在肝、小肠和大肠均有分布,这表明Ghrelin可能对消化有一定的调节作用。 相似文献
15.
Iu D Ivashchenko I T Gut L A Osipova L V Garmanchuk L N Khranovskaia 《Biulleten' eksperimental'no? biologii i meditsiny》1986,101(4):475-478
To determine the role of the epidermal growth factor (EGF) in the regenerative stimulation of intestinal epithelium and hepatocyte proliferation after partial resection of these organs the labeling index of the intestine and liver in sialadenectomized rats was studied. EGF concentration in the saliva and serum was determined using radioimmunoassay. The decrease in EGF concentration after the removal of submandibular salivary glands was shown to slower hepatocytes entering the mitotic cycle and to inhibit the activity of enterocyte proliferation in the small intestine. The data show pronounced in vivo mitogenic effect of EGF on the liver and intestinal epithelium. 相似文献
16.
Cell-specific subcellular localization of soluble epoxide hydrolase in human tissues. 总被引:2,自引:0,他引:2
Ahmed E Enayetallah Richard A French Michele Barber David F Grant 《The journal of histochemistry and cytochemistry》2006,54(3):329-335
Soluble epoxide hydrolase (sEH) is a phase-I xenobiotic metabolizing enzyme having both an N-terminal phosphatase activity and a C-terminal epoxide hydrolase activity. Endogenous hydrolase substrates include arachidonic acid epoxides, which have been involved in regulating blood pressure and inflammation. The subcellular localization of sEH has been controversial. Earlier studies using mouse and rat liver suggested that sEH may be cytosolic and/or peroxisomal. In this study we applied immunofluorescence and confocal microscopy using markers for different subcellular compartments to evaluate sEH colocalization in an array of human tissues. Results showed that sEH is both cytosolic and peroxisomal in human hepatocytes and renal proximal tubules and exclusively cytosolic in other sEH-containing tissues such as pancreatic islet cells, intestinal epithelium, anterior pituitary cells, adrenal gland, endometrium, lymphoid follicles, prostate ductal epithelium, alveolar wall, and blood vessels. sEH was not exclusively peroxisomal in any of the tissues evaluated. Our data suggest that human sEH subcellular localization is tissue dependent, and that sEH may have tissue- or cell-type-specific functionality. To our knowledge, this is the first report showing the subcellular localization of sEH in a wide array of human tissues. 相似文献
17.
Kostiukevich SV 《Tsitologiia》2003,45(6):549-554
The epithelium of the distal part of Rana temporaria intestine has been studied by light and electron microscopy. It has been shown that the number of agrent-affined endocrinocytes in the mucosa of the frog intestinal epithelium decreases gradually from the small bowel to the colon, and then sharply increased in the distal part of colon (cloaca). Four types of endocrinocytes have been identified: EC, D, L and type IV. In frogs examined in March, endocrinocytes of the colon mucosal epithelium demonstrate structural features characteristic of the state of functional strain. These features are most pronounced in EC-cells. This seems to be due to the state of awakening from hibernation, serotonin (produced by EC-cells) playing an essential role in this process. 相似文献
18.
Summary A possible contribution of the intestinal epithelium to the immune defence system was studied by electron microscopy in the rat small intestine. The cells of the immune system (CIS) such as lymphocytes, eosinophils and macrophages penetrate the basal lamina into the epithelium and make close relationships with the absorptive cells. At the points of close apposition, the two cell membranes run parallel at a regular distance of about 20 nm. On the other hand, about 5% of the intestinal absorptive cells also penetrate the basal lamina into the lamina propria via their basal protrusions and show a similar close association with CIS. The basal protrusions contain many microfilaments; this indicates that they are structures with a definite function rather than a simple hernia. These findings are discussed with respect to the transport of antigenic molecules and of intercellular communication between CIS and the intestinal epithelium. 相似文献
19.
It was observed that ground substance between the smooth muscle fibers in cerebral arteries stained by periodic acid-Schiff (PAS) was red as seen by ordinary bright-field microscopy (BF), but blue as observed by phase-contrast microscopy (PC). The basement membranes in the small intestine and around the kidney tubules, as well as the striated borders of the intestinal epithelium and the brush borders of the kidney tubules, were seen in blue when stained by PAS and observed by PC. The cytoplasm of PAS stained liver cells, when observed by PC, had irregular shaped areas of blue interspersed between the red material. This blue color was seen by PC after PAS, ninhydrin-Schiff and the Feulgen procedures. Our evidence suggests that this phenomena is characteristic of Schiff-positive material. Digestion by various enzymes: malt diastase, testicular hyaluronidase, collagenase, pepsin, pectinase, trypsin and DNase showed different effects on ground substance, liver cells, basement membranes, and brush and striated borders. 相似文献
20.
Moriwaki Y Yamamoto T Yamakita J Takahashi S Higashino K 《The Histochemical journal》1998,30(2):69-74
The distribution of aldehyde oxidase activity was evaluated in unfixed cryostat sections from tissues of male Wistar rats using a tissue protectant, polyvinyl alcohol, with Tetranitro BT as a final electron acceptor. The distribution of aldehyde oxidase activity was compared with that of xanthine oxidoreductase. The enzyme histochemical method demonstrated aldehyde oxidase activity in the epithelium of the tongue, renal tubules and bronchioles, as well as in the cytoplasm of liver cells. Such activity was not detected in oesophagus, stomach, spleen, adrenal glands, small or large intestine or skeletal and heart muscle fibres. In contrast, xanthine oxidoreductase activity was demonstrated in the tongue, renal tubules, bronchioles, oesophageal, gastric, small and large intestinal epithelial cells, adrenal glands, spleen and liver cytoplasm but not in skeletal and heart muscle fibres. The significance of the ubiquitous distribution of aldehyde oxidase activity, especially in surface epithelial cells from various tissues, except for the gastrointestinal tract, is unclear. However, aldehyde oxidase may possess some physiological activity other than in the metabolism of N-heterocyclics or of certain drugs. © 1998 Chapman & Hall 相似文献