共查询到20条相似文献,搜索用时 15 毫秒
1.
Jack William Houghton Guy Carpenter Joachim Hans Manuel Pesaro Steven Lynham Gordon Proctor 《Molecular & cellular proteomics : MCP》2020,19(10):1664-1676
Highlights
- •Salivary secretion was increased by mouth rinsing with TRP channel agonists.
- •The salivary proteome varied over time and was changed by TRP channel stimulation.
- •Immunoreactive Cystatin S was increased in saliva after TRPV1 stimulation.
2.
Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation*
《Molecular & cellular proteomics : MCP》2019,18(5):909-922
Highlights
- •Characterization of the phagosomal proteome comparing resting and LPS-treated BMDCs.
- •Label-free quantification determined 2843 phagosomal proteins.
- •Reduced recruitment of hydrolases and V-ATPase to phagosomes of LPS-treated cells.
- •Increased recruitment of antigen cross-presentation molecules to these phagosomes.
3.
《Molecular & cellular proteomics : MCP》2019,18(3):490-503
Highlights
- •MHC-II-bound peptide repertoires from DO-sufficient and DO-deficient cells.
- •Fewer unique peptides and core epitopes were presented in the absence of DO.
- •Immunopeptidome differences appeared to result from reduced DM editing.
- •DO-dependent self-epitopes elicited CD4 T cell responses in mice.
4.
《Molecular & cellular proteomics : MCP》2019,18(3):594-605
Highlights
- •A new strategy for simultaneous quantification of protein expression and modification.
- •This top-down LC/MS-based method shows high reproducibility and high throughput.
- •Quantification at the intact protein level with results comparable to Western blot.
- •This top-down proteomics method is applicable to different species and tissues.
5.
《Molecular & cellular proteomics : MCP》2019,18(9):1705-1720
Highlights
- •Quantitative changes in global proteome and ubiquitinome in Huntington's disease.
- •Differential ubiquitination of wild-type and mutant Htt in mice brain.
- •Enriched pathways include vesicle transport and mRNA processing.
- •Correlation between protein and diGly site fold changes.
6.
《Molecular & cellular proteomics : MCP》2019,18(4):606-621
Highlights
- •In-depth proteome underpins the gland ontogeny and age-specific activity of the HGs.
- •The well-developed acini in the HGs of NBs promote the RJ secretary activities.
- •The enhanced protein and energy metabolism in the HGs boost the stronger RJ secretion of RJBs.
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《Molecular & cellular proteomics : MCP》2019,18(8):1491-1510
Highlights
- •HLA-B*51 and ERAP1, but not ERAP2, are risk factors for Behçet's disease.
- •The HLA-B*51 peptidome and the effects of ERAP1 and ERAP2 on it are analyzed.
- •ERAP1 and ERAP2 alter multiple features of the HLA-B*51 peptidome in distinct ways.
- •Both enzymes act independently with complementary and partially redundant functions.
9.
《Molecular & cellular proteomics : MCP》2019,18(4):786-795
Highlights
- •Quantitative cross-linking mass spectrometry (QCLMS) was automated by Spectronaut.
- •Data-independent acquisition (DIA) was adapted to QCLMS.
- •Accuracy and precision of quantitation improves with DIA over DDA.
- •QCLMS is now ready for use in complex samples.
10.
《Molecular & cellular proteomics : MCP》2019,18(4):704-714
Highlights
- •The proteomes of L. lactis MG1363 and phage p2 at different stages of infection were characterized.
- •16% (226/1412) of the bacterial proteins detected were unique to infected cultures.
- •A targeted approach using synthetic peptides improved the coverage of phage p2 proteome.
- •By means of proteogenomics, we uncovered a conserved phage protein coded by a previously unannotated gene.
- •Deletion of the bacterial gene llmg_0219 (unknown function) impedes phage p2 infection.
11.
《Molecular & cellular proteomics : MCP》2019,18(4):657-668
Highlights
- •Global proteomic remodeling alters antibiotic susceptibility in K. pneumoniae.
- •Drug specific transport, sugar utilization, central metabolism, capsule synthesis.
- •Common pathways of reactive oxygen scavenging, turnover of misfolded proteins.
- •Integrated adjustments and unique drug-specific features for drug combinations.
12.
《Molecular & cellular proteomics : MCP》2019,18(9):1864-1879
Highlights
- •Feeding mice a western-style obesogenic diet resulted in dramatic changes in fecal microbial proteome.
- •Basal fecal microbial proteome, but not microbiome, composition was associated with extent of diet-induced obesity.
- •A major feature of fecal microbial proteome of high-responder mice was enriched motility-related proteins.
13.
《Molecular & cellular proteomics : MCP》2018,17(12):2534-2545
Highlights
- •Online PASEF achieves greater than 100 MS/MS per second at full sensitivity.
- •Accurate label-free quantification of over 6000 proteins in 2 h.
- •High throughput demonstrated on 50 ng digests measured in 5 min.
- •High-precision determination of 100,000 peptide collisional cross sections.
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《Molecular & cellular proteomics : MCP》2019,18(1):41-50
Highlights
- •Proteome of mature boar spermatozoa from cauda epididymal and ejaculated sources were analyzed by iTRAQ-based LC-MS/MS.
- •1,723 sperm proteins identified (974 of Sus scrofa taxonomy).
- •1,602 sperm proteins quantified (960 of Sus scrofa taxonomy).
- •32 Sus scrofa sperm proteins were differentially expressed among sperm sources.
- •The proteome of boar spermatozoa is remodelled during ejaculation.
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《Molecular & cellular proteomics : MCP》2019,18(10):2089-2098
Highlights
- •Cathepsin-L is introduced as a novel protease for HX-MS studies.
- •Cathepsin-L improves resolution of traditionally challenging histone tails.
- •Cathepsin-L can be readily combined with pepsin for improved protein coverage.
- •In-solution dynamics of the H3.1 and H4 monomers reveal extensive EX1 kinetics.