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1.
《Molecular & cellular proteomics : MCP》2019,18(11):2324-2334
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- •Automated analysis of protein complexes in proteomic experiments.
- •Quantitative measurement of the coordinated changes in protein complex components.
- •Interactive visualizations for exploratory analysis of proteomic results.
2.
《Molecular & cellular proteomics : MCP》2019,18(5):954-967
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- •Two-step cross-linking coupled with affinity purification to facilitate structural analysis of protein complexes.
- •Integrated QXL-MS workflow for studying condition-dependent structural changes of protein complexes.
- •Mechanistic insights on in vivo H2O2-induced conformational dynamics of proteasome complexes.
3.
《Molecular & cellular proteomics : MCP》2018,17(12):2448-2461
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- •Chemical proteomics strategy for quantitative profiling of phosphoprotein phosphatases.
- •Compatible with quantitative multiplexing approaches.
- •Applicable to many samples types including tissues from human to yeast.
4.
《Molecular & cellular proteomics : MCP》2019,18(7):1428-1436
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- •Protein interaction screen of SETD1A/COMPASS complex subunits.
- •Unexpected interaction with DNA damage protein RAD18 was confirmed for SETD1A, but not for other subunits.
- •SETD1A and/or RAD18 influence each other's mRNA and protein expression levels, and disruption of either gene elicits a similar DNA damage sensitivity phenotype.
5.
《Molecular & cellular proteomics : MCP》2019,18(7):1437-1453
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- •mRNA-seq, miRNA-seq, proteomes of P. fulvidraco, P. vachelli, hybrid Huangyou-1.
- •Predicted miRNA-mRNA-protein pairs were found and validated by qRT-PCR and PRM.
- •Immune, metabolism, digestion, absorption, proliferation, development generate heterosis.
- •High parental gene/protein with low parental miRNAs inherit from the mother or father.
6.
《Molecular & cellular proteomics : MCP》2019,18(12):2516-2523
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- •Open source software for comprehensive HDX-MS data analysis.
- •Automatic back-exchange correction options.
- •Rigorous statistical analysis of the significance of uptake differences.
- •High quality visualization tools.
7.
《Molecular & cellular proteomics : MCP》2019,18(2):308-319
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- •Over 1700 Arabidopsis proteins with thermal models in multiple replicates.
- •Melting temperature correlates with 1°, 2°, and 3° protein characteristics.
- •Ligand-induced thermal shifts are evident in complex protein extracts.
8.
《Molecular & cellular proteomics : MCP》2018,17(12):2518-2533
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- •Chromobodies are stabilized by antigen binding in live cells.
- •Monitoring changes of endogenous protein levels in living cells with chromobodies.
- •Broadly applicable system to generate turnover-accelerated chromobodies.
- •Quantification of time- and dose-dependent compound effects.
9.
《Molecular & cellular proteomics : MCP》2019,18(6):1110-1122
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- •Comprehensive analysis of inter-individual variation of normal urinary proteome.
- •Significant gender differences were observed.
- •Proteins increased in female urine are enriched in immunological pathways.
- •Estimated reference intervals of proteins as the baseline for biomarker discovery.
10.
《Molecular & cellular proteomics : MCP》2019,18(8):1479-1490
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- •Frequent genetic polymorphism affects the glycosylation pattern of fetuin.
- •Personalized in-depth proteoform profiling of fetuin purified from 20 donors.
- •Classification of serum donors into three different genotypes.
- •Septic patients show increased level of fucosylation at N-glycolation site N176.
11.
《Molecular & cellular proteomics : MCP》2019,18(8):1556-1571
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- •Functional role of a yet uncharacterized receptor kinase QSK1.
- •Activation model for SIRK1 receptor kinase in a heteromer with QSK1.
- •Role of QSK1 in substrate recruitment and stabilization of the complex.
12.
《Molecular & cellular proteomics : MCP》2019,18(4):786-795
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- •Quantitative cross-linking mass spectrometry (QCLMS) was automated by Spectronaut.
- •Data-independent acquisition (DIA) was adapted to QCLMS.
- •Accuracy and precision of quantitation improves with DIA over DDA.
- •QCLMS is now ready for use in complex samples.
13.
《Molecular & cellular proteomics : MCP》2019,18(11):2207-2224
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- •Quantitative proteomes and epigenetic regulation of T. gondii.
- •Protein crotonylation and 2-hydroxyisobutylation in phenotypically different T. gondii parasites.
- •Regulation of invasion regulation of T. gondii by protein modification.
- •Lysine crotonylation and 2-hydroxyisobutylation regulated in multiple biological processes in phenotypically different T. gondii parasites.
14.
《Molecular & cellular proteomics : MCP》2019,18(10):2089-2098
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- •Cathepsin-L is introduced as a novel protease for HX-MS studies.
- •Cathepsin-L improves resolution of traditionally challenging histone tails.
- •Cathepsin-L can be readily combined with pepsin for improved protein coverage.
- •In-solution dynamics of the H3.1 and H4 monomers reveal extensive EX1 kinetics.
15.
《Molecular & cellular proteomics : MCP》2019,18(4):773-785
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- •The developed Ac-LysargiNase showed higher stability and activity than before.
- •The merged spectra of the mirror peptides achieved nearly complete ion coverage.
- •pNovoM obviously increased the efficiency and accuracy of peptide sequencing.
- •The mirror enzymatic strategy achieved precision de novo sequencing on proteome scales.
16.
《Molecular & cellular proteomics : MCP》2019,18(8):1630-1650
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- •Our search identifies 2,134 kinase-substrate phosphosite pairs in breast cancer.
- •CDKs and MAPKs are dominant regulators of trans substrate-phosphorylation.
- •Druggability, outcomes, and immune signatures related to kinase-substrates.
- •Experimentally validated activated phosphosites of ERBB2, EIF4EBP1, and EGFR.
17.
《Molecular & cellular proteomics : MCP》2019,18(1):41-50
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- •Proteome of mature boar spermatozoa from cauda epididymal and ejaculated sources were analyzed by iTRAQ-based LC-MS/MS.
- •1,723 sperm proteins identified (974 of Sus scrofa taxonomy).
- •1,602 sperm proteins quantified (960 of Sus scrofa taxonomy).
- •32 Sus scrofa sperm proteins were differentially expressed among sperm sources.
- •The proteome of boar spermatozoa is remodelled during ejaculation.
18.
《Molecular & cellular proteomics : MCP》2019,18(10):2078-2088
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- •Proteomic landscapes of Drosophila somatic and reproductive tissues during aging.
- •Pulsed metabolic labeling determines a decline in protein synthesis with age.
- •Drosophila model of human Parkinson's disease signifies an early-onset decline in protein synthesis.
- •Collapse of proteostasis and mitochondria are early signals for normal aging.
19.
《Molecular & cellular proteomics : MCP》2019,18(5):854-864
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- •Zero-length chemical cross-linking of APOA1 peptides in HDL.
- •Cross-links match antiparallel isomers of APOA dimers in molecular modeling.
- •Identical MS/MS spectra of native and synthetic cross-linked peptides.
- •First biochemical evidence of LL5/5 and LL5/4 isomers in human HDL.
20.
《Molecular & cellular proteomics : MCP》2019,18(10):2058-2077
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- •Identification of the substrates profile of the endothelial phosphatase VE-PTP.
- •A large fraction of VE-PTP substrate candidates (29%) is cell junction related.
- •Tie-2 and EPHB are substrates which associate as ternary complex with VE-PTP.