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1.
《Molecular & cellular proteomics : MCP》2019,18(9):1732-1744
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- •An innovative co-IP crosslinking proteomics study was designed for the TLR2 interactome.
- •Proteomic profiling revealed combinatorial effects of simvastatin and Pam3CSK4 on the TLR2 interactome.
- •ACTR1A and MARCKSL1 proteins were identified as potential interactors of TLR2 during the immune response.
- •ACTR1A has important modulatory actions on the TLR2 pro-inflammatory signaling cascade.
2.
《Molecular & cellular proteomics : MCP》2019,18(4):669-685
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- •quantitative phosphoproteome analysis of TDM-activated macrophages.
- •distinct Mincle-dependent and independent phosphorylation and gene regulations.
- •Mincle-dependent activation of PI3K/AKT signaling by TDM.
- •Mincle-independent macrophage response is linked to cell cycle regulation.
3.
《Molecular & cellular proteomics : MCP》2018,17(12):2496-2507
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- •Quantitative (phospho)proteome analysis of antibiotic treatment in E. coli.
- •Largest bacterial phosphorylation catalogue.
- •Specific phosphorylation motifs changes during resistance development.
- •Phosphorylation mediated signaling could be a potential target for drug design.
4.
《Molecular & cellular proteomics : MCP》2019,18(11):2324-2334
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- •Automated analysis of protein complexes in proteomic experiments.
- •Quantitative measurement of the coordinated changes in protein complex components.
- •Interactive visualizations for exploratory analysis of proteomic results.
5.
《Molecular & cellular proteomics : MCP》2019,18(3):520-533
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- •1. Celastrol alleviated cholestatic liver injury.
- •2. Celastrol inhibited the decrease of SIRT1 induced by deoxycholic acid.
- •3. SIRT1-FXR signaling pathway mediated the effect of celastrol.
6.
《Molecular & cellular proteomics : MCP》2019,18(6):1096-1109
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- •Quantitative phosphoproteome of BRAF drug-resistance in melanoma cells.
- •Cytoskeletal proteins are downregulated in resistant vs. sensitive cells.
- •Nestin is associated with an invasive phenotype and resistance to MEK and BRAF inhibitors.
- •Nestin depletion affects PI3K/AKT and integrin signaling similar to resistant cells.
7.
《Molecular & cellular proteomics : MCP》2019,18(12):2516-2523
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- •Open source software for comprehensive HDX-MS data analysis.
- •Automatic back-exchange correction options.
- •Rigorous statistical analysis of the significance of uptake differences.
- •High quality visualization tools.
8.
Intact Transition Epitope Mapping – Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE),
《Molecular & cellular proteomics : MCP》2019,18(8):1543-1555
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- •Multiplex epitope mapping/antigenic determinant identification in the gas phase.
- •Intact transition and controlled dissociation of immune complexes by MS.
- •Simultaneous identification and amino acid sequence determination of epitopes.
- •Simplified in-solution sample handling because of ion manipulation and filtering by MS.
9.
《Molecular & cellular proteomics : MCP》2019,18(5):936-953
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- •In-depth proteome profiling of primary human myeloma cells
- •Characteristics of myeloma cells are related to hypoxic bone marrow conditions
- •Myeloma cells show specific immune evasion strategies
- •Metabolic adaptations involve tumor and stroma cells
10.
《Molecular & cellular proteomics : MCP》2018,17(12):2371-2386
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- •Stability of oxidative phosphorylation subunits are reduced in a diet-induced mouse model of NAFLD.
- •These changes are associated with impaired activities of electron transport chain complexes and ATP synthesis.
- •Increased mitophagy contributed to enhanced degradation of mitochondrial proteins.
11.
《Molecular & cellular proteomics : MCP》2019,18(12):2348-2358
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- •Glycosylation is not currently considered in flu vaccine design.
- •Glycosylation influences on immunodominance are not well understood.
- •Identification of site-specific glycosylation using mass spectrometry has matured.
- •New methods are needed to quantify site-specific glycosylation for vaccine design.
12.
《Molecular & cellular proteomics : MCP》2019,18(3):576-593
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- •Database of PTM site-specific phosphorylation signatures of kinases, perturbations and signaling pathways (PTMsigDB).
- •PTM signature enrichment analysis (PTM-SEA) outperformed gene-centric analysis in detection of EGF induced phospho signaling events.
- •PI3K perturbation signatures were readily detected in PI3Ka inhibited human breast cancer cells.
- •PTMsigDB and PTM-SEA can be freely accessed at https://github.com/broadinstitute/ssGSEA2.0.
13.
《Molecular & cellular proteomics : MCP》2019,18(5):982-994
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- •MaxQuant.Live controls Orbitrap mass analyzers in real-time.
- •Freely available apps enable advanced data acquisition strategies.
- •On-the-fly mass, retention time and intensity recalibration.
- •Global targeting unifies shotgun and targeted proteomics.
14.
《Molecular & cellular proteomics : MCP》2019,18(2):231-244
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- •OMICS distinguish cancer cells from resistant or cancer stem cells.
- •Bactericidal antibiotics and mitochondria.
- •Linezolid and anticancer therapy.
15.
《Molecular & cellular proteomics : MCP》2019,18(9):1705-1720
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- •Quantitative changes in global proteome and ubiquitinome in Huntington's disease.
- •Differential ubiquitination of wild-type and mutant Htt in mice brain.
- •Enriched pathways include vesicle transport and mRNA processing.
- •Correlation between protein and diGly site fold changes.
16.
《Molecular & cellular proteomics : MCP》2019,18(5):954-967
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- •Two-step cross-linking coupled with affinity purification to facilitate structural analysis of protein complexes.
- •Integrated QXL-MS workflow for studying condition-dependent structural changes of protein complexes.
- •Mechanistic insights on in vivo H2O2-induced conformational dynamics of proteasome complexes.
17.
《Molecular & cellular proteomics : MCP》2019,18(9):1796-1806
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- •iTRAQ-based analysis of saliva samples from oral cancer patients.
- •Proteome profiling of saliva samples from patients with oral premalignant lesions.
- •Verification of salivary biomarker candidates with MRM-MS and immunoassays.
- •Identification of salivary proteins as potential biomarkers of oral cancer.
18.
《Molecular & cellular proteomics : MCP》2018,17(12):2518-2533
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- •Chromobodies are stabilized by antigen binding in live cells.
- •Monitoring changes of endogenous protein levels in living cells with chromobodies.
- •Broadly applicable system to generate turnover-accelerated chromobodies.
- •Quantification of time- and dose-dependent compound effects.
19.
《Molecular & cellular proteomics : MCP》2019,18(11):2285-2297
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- •BioID with Golgi fractions identified C10orf76 as proximal to GBF1.
- •Tagged C10orf76 overlaps with Golgi markers.
- •C10orf76 binds GBF1 and exchanges rapidly between free and bound forms.
- •C10orf76 is essential for maintenance of the Golgi and for secretion.
20.
《Molecular & cellular proteomics : MCP》2019,18(12):2524-2531
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- •Efficient sample preparation workflow for deep N-glycomics analysis from serum.
- •Temperature gradient denaturing protocol to prevent protein precipitation.
- •Decrease of free sugar content in serum enhanced PNGase F digestion efficiency.
- •Modified evaporative labeling method increased fluorophore labeling yield.