共查询到20条相似文献,搜索用时 15 毫秒
1.
《Molecular & cellular proteomics : MCP》2019,18(9):1732-1744
Highlights
- •An innovative co-IP crosslinking proteomics study was designed for the TLR2 interactome.
- •Proteomic profiling revealed combinatorial effects of simvastatin and Pam3CSK4 on the TLR2 interactome.
- •ACTR1A and MARCKSL1 proteins were identified as potential interactors of TLR2 during the immune response.
- •ACTR1A has important modulatory actions on the TLR2 pro-inflammatory signaling cascade.
2.
《Molecular & cellular proteomics : MCP》2019,18(4):669-685
Highlights
- •quantitative phosphoproteome analysis of TDM-activated macrophages.
- •distinct Mincle-dependent and independent phosphorylation and gene regulations.
- •Mincle-dependent activation of PI3K/AKT signaling by TDM.
- •Mincle-independent macrophage response is linked to cell cycle regulation.
3.
《Molecular & cellular proteomics : MCP》2018,17(12):2496-2507
Highlights
- •Quantitative (phospho)proteome analysis of antibiotic treatment in E. coli.
- •Largest bacterial phosphorylation catalogue.
- •Specific phosphorylation motifs changes during resistance development.
- •Phosphorylation mediated signaling could be a potential target for drug design.
4.
《Molecular & cellular proteomics : MCP》2019,18(3):520-533
Highlights
- •1. Celastrol alleviated cholestatic liver injury.
- •2. Celastrol inhibited the decrease of SIRT1 induced by deoxycholic acid.
- •3. SIRT1-FXR signaling pathway mediated the effect of celastrol.
5.
《Molecular & cellular proteomics : MCP》2019,18(11):2324-2334
Highlights
- •Automated analysis of protein complexes in proteomic experiments.
- •Quantitative measurement of the coordinated changes in protein complex components.
- •Interactive visualizations for exploratory analysis of proteomic results.
6.
《Molecular & cellular proteomics : MCP》2019,18(6):1096-1109
Highlights
- •Quantitative phosphoproteome of BRAF drug-resistance in melanoma cells.
- •Cytoskeletal proteins are downregulated in resistant vs. sensitive cells.
- •Nestin is associated with an invasive phenotype and resistance to MEK and BRAF inhibitors.
- •Nestin depletion affects PI3K/AKT and integrin signaling similar to resistant cells.
7.
《Molecular & cellular proteomics : MCP》2019,18(12):2516-2523
Highlights
- •Open source software for comprehensive HDX-MS data analysis.
- •Automatic back-exchange correction options.
- •Rigorous statistical analysis of the significance of uptake differences.
- •High quality visualization tools.
8.
Intact Transition Epitope Mapping – Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE),
《Molecular & cellular proteomics : MCP》2019,18(8):1543-1555
Highlights
- •Multiplex epitope mapping/antigenic determinant identification in the gas phase.
- •Intact transition and controlled dissociation of immune complexes by MS.
- •Simultaneous identification and amino acid sequence determination of epitopes.
- •Simplified in-solution sample handling because of ion manipulation and filtering by MS.
9.
《Molecular & cellular proteomics : MCP》2019,18(5):936-953
Highlights
- •In-depth proteome profiling of primary human myeloma cells
- •Characteristics of myeloma cells are related to hypoxic bone marrow conditions
- •Myeloma cells show specific immune evasion strategies
- •Metabolic adaptations involve tumor and stroma cells
10.
《Molecular & cellular proteomics : MCP》2018,17(12):2371-2386
Highlights
- •Stability of oxidative phosphorylation subunits are reduced in a diet-induced mouse model of NAFLD.
- •These changes are associated with impaired activities of electron transport chain complexes and ATP synthesis.
- •Increased mitophagy contributed to enhanced degradation of mitochondrial proteins.
11.
《Molecular & cellular proteomics : MCP》2019,18(12):2348-2358
Highlights
- •Glycosylation is not currently considered in flu vaccine design.
- •Glycosylation influences on immunodominance are not well understood.
- •Identification of site-specific glycosylation using mass spectrometry has matured.
- •New methods are needed to quantify site-specific glycosylation for vaccine design.
12.
《Molecular & cellular proteomics : MCP》2019,18(5):982-994
Highlights
- •MaxQuant.Live controls Orbitrap mass analyzers in real-time.
- •Freely available apps enable advanced data acquisition strategies.
- •On-the-fly mass, retention time and intensity recalibration.
- •Global targeting unifies shotgun and targeted proteomics.
13.
《Molecular & cellular proteomics : MCP》2019,18(3):576-593
Highlights
- •Database of PTM site-specific phosphorylation signatures of kinases, perturbations and signaling pathways (PTMsigDB).
- •PTM signature enrichment analysis (PTM-SEA) outperformed gene-centric analysis in detection of EGF induced phospho signaling events.
- •PI3K perturbation signatures were readily detected in PI3Ka inhibited human breast cancer cells.
- •PTMsigDB and PTM-SEA can be freely accessed at https://github.com/broadinstitute/ssGSEA2.0.
14.
《Molecular & cellular proteomics : MCP》2019,18(2):231-244
Highlights
- •OMICS distinguish cancer cells from resistant or cancer stem cells.
- •Bactericidal antibiotics and mitochondria.
- •Linezolid and anticancer therapy.
15.
《Molecular & cellular proteomics : MCP》2019,18(5):954-967
Highlights
- •Two-step cross-linking coupled with affinity purification to facilitate structural analysis of protein complexes.
- •Integrated QXL-MS workflow for studying condition-dependent structural changes of protein complexes.
- •Mechanistic insights on in vivo H2O2-induced conformational dynamics of proteasome complexes.
16.
《Molecular & cellular proteomics : MCP》2019,18(9):1705-1720
Highlights
- •Quantitative changes in global proteome and ubiquitinome in Huntington's disease.
- •Differential ubiquitination of wild-type and mutant Htt in mice brain.
- •Enriched pathways include vesicle transport and mRNA processing.
- •Correlation between protein and diGly site fold changes.
17.
《Molecular & cellular proteomics : MCP》2019,18(9):1796-1806
Highlights
- •iTRAQ-based analysis of saliva samples from oral cancer patients.
- •Proteome profiling of saliva samples from patients with oral premalignant lesions.
- •Verification of salivary biomarker candidates with MRM-MS and immunoassays.
- •Identification of salivary proteins as potential biomarkers of oral cancer.
18.
《Molecular & cellular proteomics : MCP》2018,17(12):2518-2533
Highlights
- •Chromobodies are stabilized by antigen binding in live cells.
- •Monitoring changes of endogenous protein levels in living cells with chromobodies.
- •Broadly applicable system to generate turnover-accelerated chromobodies.
- •Quantification of time- and dose-dependent compound effects.
19.
《Molecular & cellular proteomics : MCP》2019,18(12):2524-2531
Highlights
- •Efficient sample preparation workflow for deep N-glycomics analysis from serum.
- •Temperature gradient denaturing protocol to prevent protein precipitation.
- •Decrease of free sugar content in serum enhanced PNGase F digestion efficiency.
- •Modified evaporative labeling method increased fluorophore labeling yield.
20.
《Molecular & cellular proteomics : MCP》2019,18(11):2149-2164
Highlights
- •Enrichment of methyl peptides using two orthogonal techniques.
- •Knockdown of PRMT1 leads to substantial changes in protein arginine “methylome”.
- •Discrimination of ADMA and SDMA using characteristic neutral losses.
- •Identification of PRMT1 targets and substrate scavenged by other PRMTs in the absence of PRMT1 activity.