An experimental study using tissue culture medium as preservation and perfusion fluid

A commercially available tissue culture medium has been proven capable of preserving dog kidney function for at least 24 hr after simple cooling. The advantages of using tissue culture medium as preservation fluid instead of plasma or albumin solutions from the infectious and immunological points of view are obvious. An in vitro study was completed using the tissue

1.

     

4.

An overview of coronary artery disease-1981     

Hall RJ 《Cardiovascular diseases》1981,8(2):159-164

     

5.

An experimental study of mitosis     

《Journal of biological education》2012,46(3):195-198

A comparison of colchicine-treated and normal root tips of onion provides information on the role of the spindle in mitosis, and clearly indicates the value of colchicine in the analysis of karyotypes. An improved Feulgen-squash technique is recommended, with which high quality preparations of chromosomes can be obtained as a matter of routine. This cytological exercise requires little equipment and is suitable for undergraduate courses and advanced classes in schools.     

6.

An experimental study on oletetrine     

A M Savel'eva  E K Berezina  V B Naumova 《Antibiotiki》1966,11(4):323-327

     

7.

An experimental study upon Lepidoptera     

Henry Edward Crampton 《Development genes and evolution》1899,9(2):293-318

     

8.

Sequential structural and fluid dynamic numerical simulations of a stented bifurcated coronary artery     

Morlacchi S  Chiastra C  Gastaldi D  Pennati G  Dubini G  Migliavacca F 《Journal of biomechanical engineering》2011,133(12):121010

Despite their success, stenting procedures are still associated to some clinical problems like sub-acute thrombosis and in-stent restenosis. Several clinical studies associate these phenomena to a combination of both structural and hemodynamic alterations caused by stent implantation. Recently, numerical models have been widely used in the literature to investigate stenting procedures but always from either a purely structural or fluid dynamic point of view. The aim of this work is the implementation of sequential structural and fluid dynamic numerical models to provide a better understanding of stenting procedures in coronary bifurcations. In particular, the realistic geometrical configurations obtained with structural simulations were used to create the fluid domains employed within transient fluid dynamic analyses. This sequential approach was applied to investigate the final kissing balloon (FKB) inflation during the provisional side branch technique. Mechanical stresses in the arterial wall and the stent as well as wall shear stresses along the arterial wall were examined before and after the FKB deployment. FKB provoked average mechanical stresses in the arterial wall almost 2.5 times higher with respect to those induced by inflation of the stent in the main branch only. Results also enlightened FKB benefits in terms of improved local blood flow pattern for the side branch access. As a drawback, the FKB generates a larger region of low wall shear stress. In particular, after FKB the percentage of area characterized by wall shear stresses lower than 0.5?Pa was 79.0%, while before the FKB it was 62.3%. For these reasons, a new tapered balloon dedicated to bifurcations was proposed. The inclusion of the modified balloon has reduced the mechanical stresses in the proximal arterial vessel to 40% and the low wall shear stress coverage area to 71.3%. In conclusion, these results show the relevance of the adopted sequential approach to study the wall mechanics and the hemodynamics created by stent deployment.     

9.

An experimental evaluation of skin graft preservation with silicone fluid     

D L Ballantyne  G A Hawthorne  T D Rees  I Seidman 《Cryobiology》1971,8(2):211-215

     

10.

An experimental study of mesenteric occlusion     

KHANNA SD 《The Journal of pathology and bacteriology》1959,77(2):575-590

     

11.

An experimental study of fat necrosis     

PANABOKKE RG 《The Journal of pathology and bacteriology》1958,75(2):319-331

     

12.

Effects of bifurcation-specific and conventional stents on coronary bifurcation flow. An experimental and numerical study     

《Journal of biomechanics》2017

Our paper builds on existing research into conventional bare metal stents in order to assess new devices specifically designed for coronary bifurcation angioplasty. The first aim is to validate the numerical model against data from in vitro experiments on stented coronary phantoms. A surface mesh was built in accordance with micro-computed tomography images obtained from coronary stents implanted in silicone models and used for numerical analysis. Computational simulations for steady and unsteady cases generally agreed with their experimental counterparts. A second objective is to compare the hemodynamic performance of one of these new devices (Stentys) to that of conventional devices and stenting techniques in a simplified coronary bifurcation model. Four different coronary bifurcation stenting techniques were analyzed. We have focused on factors contributing to restenosis, such as wall shear stress (WSS), oscillatory shear index (OSI), pressure loss, and local normalized helicity (LNH). It was found that bifurcation-specific stents implanted in the side branch led to increased malapposition. This effect has proved to be more important than stent specific design characteristics such as strut size (different for conventional and Stentys stent). This conclusion is confirmed by means of drop in pressure and mechanical energy loss rate calculation; for the latter, the increase ranged from 9% to 17%, depending on the stenting technique, when dedicated stents were implanted in the side branch. The behavior patterns presented in this study should be double-checked against those obtained in more realistic geometries.     

13.

Computer modeling of fluid dynamics related to a myocardial bridge in a coronary artery   总被引：2，自引：0，他引：2  

Liu H  Yamaguchi T 《Biorheology》1999,36(5-6):373-390

Fluid mechanics associated with blood flows induced by the so-called myocardial bridge (MB) has been studied systematically using a computational fluid dynamic modeling of the Newtonian, incompressible, two-dimensional, unsteady flow in a channel with a time-dependently flushing in/out indentation. During each cycle, a train of vortex wave flow was observed downstream of the phasic stenosis and both upper and lower walls suffer severely from consistently high, oscillating wall shear stresses (WSS). Extensive studies were conducted on the influence of the Reynolds number, the geometry and the Strouhal number of the MB movement on the nature of the vortex flow and the time-dependent wall shear stress distribution. Special attention was drawn to the relationship between the vortex wave and the pressure distribution. It was found that the pressure gradient changed markedly during one cycle, which was apparently dominated by the dynamics of the indentation. A steep, adverse pressure gradient was observed when the indentation was flushing out, which corresponded to the existence of the most developing vortices. It implies the possibility that the MB in a coronary artery can produce an extremely low pressure region immediately downstream of the phasic stenosis, where elastic choking or collapse of the coronary artery might occur.     

14.

A study of the orifice of the human coronary artery   总被引：1，自引：0，他引：1  

D H Blankenhorn 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1967,126(2):418-420

     

15.

An electron microscope study of coronary arteries   总被引：4，自引：0，他引：4  

PARKER F 《The American journal of anatomy》1958,103(2):247-273

     

16.

生物反应器填埋场的试验研究   总被引：6，自引：2，他引：6  

王君琴  沈东升 《应用生态学报》2003,14(11):2077-2078

In this study, a methane bioreactor-landfill system was utilized to treat municipal solid waste (MSW). Through analyzing and detecting the pollutant(CODcr) in the bioreactor-landfill system, a simulated mathematic formulaof waste degradation was established. After treated with this system, the CODcr and VFA concentrations in MSW could be decreased from more than 20000 and 7000 mg·L^-1 to less than 1500 and 200 mg·L^-1, respec-tively.     

17.

An experimental study of wrinkled laminar flame     

《Symposium (International) on Combustion》1981,18(1):931-939

     

18.

An experimental study of potentiated aqueous solutions     

Lobyshev VI  Tomkevich MS  Petrushanko IIu 《Biofizika》2005,50(3):464-469

A systematic study was undertaken of luminescent aqueous solutions of homeopathic preparation of sodium chloride at a dilution from D1 to D30, produced by "Weleda" company (Moscow) was carried out. It was shown that intensity of luminescence versus the degree of dilution is a non-monotonous function with several maxima, the main maximum corresponds to 13-14 decimal dilution. The dynamics of spectra was registered for several weeks. A systematic study of water samples (D1-D30) exposed to a similar procedure of potentization but without salt addition was also performed. The difference in the luminescence spectra of water of different stages of potentization was shown. The motility of infusoria Spirostoma ambiquum in solutions being examined was studied. A significant negative correlation between the infusoria motility and luminescence intensity was registered.     

19.

An experimental study of a modified turbidostat     

Pramod Agrawal 《Biotechnology Techniques》1987,1(1):19-24

Summary The efficacy of a modified turbidostat scheme for control of a continuous fermentor supporting substrate - inhibited growth of a methylotroph, L3, was experimentally examined. The control was based on continuous measure of optical density of fermentation broth. The experimental results illustrate the feasibility of the control scheme for effective startup as well as stabilization of a continuous fermentor under the influence of a load disturbance.     

20.

An experimental study of regeneration in cartilage     

RAO KV 《The Journal of pathology and bacteriology》1954,67(2):455-459

     

Code (animal No.)	Perfusion time (br)	Perfusion pressure mm/Hg	Flow ml/min	Weight gain	pH	pO2 mm/Hg	Histological appearance
1	24	70-60 systolic	96	35	7.3	150–180	Grossly normal
2	24		45-40 diastolic	108	30
3	24		96	30
4	48	70-60 systolic	80	35	7.3	150–180	Grossly normal
5	48	45-40 diastolic	120	40	7.4
6	48		100	40
7	72	70-60 systolic	115	40	7.4	150–180	Slight vacuolization of the tubular cells
8	72	45-40 diastolic	96	40
9	72		80	40
10	24	70-60 systolic	110	35	7.3	150–180	Used for transplantation
11	24	45-40 diastolic	120	35
12	24		140	40
13	24		100	30
14	24		96	30

An experimental study of mitosis

A comparison of colchicine-treated and normal root tips of onion provides information on the role of the spindle in mitosis, and clearly indicates the value of colchicine in the analysis of karyotypes. An improved Feulgen-squash technique is recommended, with which high quality preparations of chromosomes can be obtained as a matter of routine. This cytological exercise requires little equipment and is suitable for undergraduate courses and advanced classes in schools.     

Sequential structural and fluid dynamic numerical simulations of a stented bifurcated coronary artery

Despite their success, stenting procedures are still associated to some clinical problems like sub-acute thrombosis and in-stent restenosis. Several clinical studies associate these phenomena to a combination of both structural and hemodynamic alterations caused by stent implantation. Recently, numerical models have been widely used in the literature to investigate stenting procedures but always from either a purely structural or fluid dynamic point of view. The aim of this work is the implementation of sequential structural and fluid dynamic numerical models to provide a better understanding of stenting procedures in coronary bifurcations. In particular, the realistic geometrical configurations obtained with structural simulations were used to create the fluid domains employed within transient fluid dynamic analyses. This sequential approach was applied to investigate the final kissing balloon (FKB) inflation during the provisional side branch technique. Mechanical stresses in the arterial wall and the stent as well as wall shear stresses along the arterial wall were examined before and after the FKB deployment. FKB provoked average mechanical stresses in the arterial wall almost 2.5 times higher with respect to those induced by inflation of the stent in the main branch only. Results also enlightened FKB benefits in terms of improved local blood flow pattern for the side branch access. As a drawback, the FKB generates a larger region of low wall shear stress. In particular, after FKB the percentage of area characterized by wall shear stresses lower than 0.5?Pa was 79.0%, while before the FKB it was 62.3%. For these reasons, a new tapered balloon dedicated to bifurcations was proposed. The inclusion of the modified balloon has reduced the mechanical stresses in the proximal arterial vessel to 40% and the low wall shear stress coverage area to 71.3%. In conclusion, these results show the relevance of the adopted sequential approach to study the wall mechanics and the hemodynamics created by stent deployment.     

Effects of bifurcation-specific and conventional stents on coronary bifurcation flow. An experimental and numerical study

Our paper builds on existing research into conventional bare metal stents in order to assess new devices specifically designed for coronary bifurcation angioplasty. The first aim is to validate the numerical model against data from in vitro experiments on stented coronary phantoms. A surface mesh was built in accordance with micro-computed tomography images obtained from coronary stents implanted in silicone models and used for numerical analysis. Computational simulations for steady and unsteady cases generally agreed with their experimental counterparts. A second objective is to compare the hemodynamic performance of one of these new devices (Stentys) to that of conventional devices and stenting techniques in a simplified coronary bifurcation model. Four different coronary bifurcation stenting techniques were analyzed. We have focused on factors contributing to restenosis, such as wall shear stress (WSS), oscillatory shear index (OSI), pressure loss, and local normalized helicity (LNH). It was found that bifurcation-specific stents implanted in the side branch led to increased malapposition. This effect has proved to be more important than stent specific design characteristics such as strut size (different for conventional and Stentys stent). This conclusion is confirmed by means of drop in pressure and mechanical energy loss rate calculation; for the latter, the increase ranged from 9% to 17%, depending on the stenting technique, when dedicated stents were implanted in the side branch. The behavior patterns presented in this study should be double-checked against those obtained in more realistic geometries.     

Computer modeling of fluid dynamics related to a myocardial bridge in a coronary artery

Fluid mechanics associated with blood flows induced by the so-called myocardial bridge (MB) has been studied systematically using a computational fluid dynamic modeling of the Newtonian, incompressible, two-dimensional, unsteady flow in a channel with a time-dependently flushing in/out indentation. During each cycle, a train of vortex wave flow was observed downstream of the phasic stenosis and both upper and lower walls suffer severely from consistently high, oscillating wall shear stresses (WSS). Extensive studies were conducted on the influence of the Reynolds number, the geometry and the Strouhal number of the MB movement on the nature of the vortex flow and the time-dependent wall shear stress distribution. Special attention was drawn to the relationship between the vortex wave and the pressure distribution. It was found that the pressure gradient changed markedly during one cycle, which was apparently dominated by the dynamics of the indentation. A steep, adverse pressure gradient was observed when the indentation was flushing out, which corresponded to the existence of the most developing vortices. It implies the possibility that the MB in a coronary artery can produce an extremely low pressure region immediately downstream of the phasic stenosis, where elastic choking or collapse of the coronary artery might occur.     

生物反应器填埋场的试验研究

In this study, a methane bioreactor-landfill system was utilized to treat municipal solid waste (MSW). Through analyzing and detecting the pollutant(CODcr) in the bioreactor-landfill system, a simulated mathematic formulaof waste degradation was established. After treated with this system, the CODcr and VFA concentrations in MSW could be decreased from more than 20000 and 7000 mg·L^-1 to less than 1500 and 200 mg·L^-1, respec-tively.     

An experimental study of potentiated aqueous solutions

A systematic study was undertaken of luminescent aqueous solutions of homeopathic preparation of sodium chloride at a dilution from D1 to D30, produced by "Weleda" company (Moscow) was carried out. It was shown that intensity of luminescence versus the degree of dilution is a non-monotonous function with several maxima, the main maximum corresponds to 13-14 decimal dilution. The dynamics of spectra was registered for several weeks. A systematic study of water samples (D1-D30) exposed to a similar procedure of potentization but without salt addition was also performed. The difference in the luminescence spectra of water of different stages of potentization was shown. The motility of infusoria Spirostoma ambiquum in solutions being examined was studied. A significant negative correlation between the infusoria motility and luminescence intensity was registered.     

An experimental study of a modified turbidostat

Summary The efficacy of a modified turbidostat scheme for control of a continuous fermentor supporting substrate - inhibited growth of a methylotroph, L3, was experimentally examined. The control was based on continuous measure of optical density of fermentation broth. The experimental results illustrate the feasibility of the control scheme for effective startup as well as stabilization of a continuous fermentor under the influence of a load disturbance.