Photosynthetic responses of apricot (<Emphasis Type="Italic">Prunus armeniaca</Emphasis> L.) to photosynthetic photon flux density,leaf temperature,and CO<Subscript>2</Subscript> concentration

Two cultivars (Katy and Erhuacao) of apricot (Prunus armeniaca L.) were evaluated under open-field and solar-heated greenhouse conditions in northwest China, to determine the effect of photosynthetic photon flux density (PPFD), leaf temperature, and CO₂ concentration on the net photosynthetic rate (P _N). In greenhouse, Katy registered 28.3 μmol m⁻² s⁻¹ for compensation irradiance and 823 μmol m⁻² s⁻¹ for saturation irradiance, which were 73 and 117 % of those required by Erhuacao, respectively. The optimum temperatures for cvs. Katy and Erhuacao were 25 and 35 °C in open-field and 22 and 30 °C in greenhouse, respectively. At optimal temperatures, P _N of the field-grown Katy was 16.5 μmol m⁻² s⁻¹, 21 % less than for a greenhouse-grown apricot. Both cultivars responded positively to CO₂ concentrations below the CO₂ saturation concentration, whereas Katy exhibited greater P _N (18 %) and higher carboxylation efficiency (91 %) than Erhuacao at optimal CO₂ concentration. Both cultivars exhibited greater photosynthesis in solar-heated greenhouses than in open-field, but Katy performed better than Erhuacao under greenhouse conditions.     

Diurnal and seasonal trends in photosynthetic performance of <Emphasis Type="Italic">Dalbergia sissoo</Emphasis> Roxb. and <Emphasis Type="Italic">Hardwickia binata</Emphasis> Roxb. from a semi-arid ecosystem

Diurnal and seasonal trends in net photosynthetic rate (P _N), stomatal conductance (g), transpiration rate (E), vapour pressure deficit, temperature, photosynthetic photon flux density, and water use efficiency (WUE) were compared in a two-year-old Dalbergia sissoo and Hardwickia binata plantation. Mean daily maximum P _N in D. sissoo ranged from 21.40±2.60 μmol m⁻² s⁻¹ in rainy season I to 13.21±2.64 μmol m⁻² s⁻¹ in summer whereas in H. binata it was 20.04±1.20 μmol m⁻² s⁻¹ in summer and 13.64±0.16 μmol m⁻² s⁻¹ in winter. There was a linear relationship between daily maximum P _N and g _s in D. sissoo but there was no strong linear relationship between P _N and g _s in H. binata. In D. sissoo, the reduction in g _s led to a reduction in both P _N and E enabling the maintenance of WUE during dry season thereby managing unfavourable environmental conditions efficiently whereas in H. binata, an increase in g _s causes an increase of P _N and E with a significant moderate WUE.     

Drought tolerance in the Mediterranean species <Emphasis Type="Italic">Quercus coccifera,Quercus ilex,Pinus halepensis</Emphasis>, and <Emphasis Type="Italic">Juniperus phoenicea</Emphasis>

We investigated the strategies of four co-occurring evergreen woody species Quercus ilex, Quercus coccifera, Pinus halepensis, and Juniperus phoenicea to cope with Mediterranean field conditions. For that purpose, stem water potential, gas exchange, chlorophyll (Chl) fluorescence, and Chl and carotenoid (Car) contents were examined. We recognized two stress periods along the year, winter with low precipitation and low temperatures that led to chronic photoinhibition, and summer, when drought coincided with high radiation, leading to an increase of dynamic photoinhibition and a decrease of pigment content. Summer photoprotection was related to non-photochemical energy dissipation, electron flow to alternative sinks other than photosynthesis, decrease of Chl content, and proportional increase of Car content. Water potential of trees with deep vertical roots (Q. coccifera, Q. ilex, and P. halepensis) mainly depended on precipitation, whereas water potential of trees with shallow roots (J. phoenicea) depended not only on precipitation but also on ambient temperature.     

Effects of water stress and nitrogen supply on leaf gas exchange and fluorescence parameters of <Emphasis Type="Italic">Sophora davidii</Emphasis> seedlings

Two-month-old seedlings of Sophora davidii were subjected to a randomized complete block design with three water (80, 40, and 20 % of water field capacity, i.e. FC₈₀, FC₄₀, and FC₂₀) and three N supply [N0: 0, Nl: 92 and Nh: 184 mg(N) kg⁻¹(soil)] regimes. Water stress produced decreased leaf area (LA) and photosynthetic pigment contents, inhibited photosynthetic efficiency, and induced photodamage in photosystem 2 (PS2), but increased specific leaf area (SLA). The decreased net photosynthetic rate (P _N) under medium water stress (FC₄₀) compared to control (FC₈₀) might result from stomatal limitations, but the decreased P _N under severe water deficit (FC₂₀) might be attributed to non-stomatal limitations. On the other hand, N supply could improve photosynthetic capacity by increasing LA and photosynthetic pigment contents, and enhancing photosynthetic efficiency under water deficit. Moreover, N supply did a little in alleviating photodamages to PS2 caused by water stress. Hence water stress was the primary limitation in photosynthetic processes of S. davidii seedlings, while the photosynthetic characters of seedlings exhibited positive responses to N supply. Appropriate N supply is recommended to improve photosynthetic efficiency and alleviate photodamage under water stress.     

Photosynthetic characteristics of an endangered species <Emphasis Type="Italic">Camellia nitidissima</Emphasis> and its widespread congener <Emphasis Type="Italic">Camellia sinensis</Emphasis>

Saturation (SI) and compensation (CI) irradiances [μmol(photon) m⁻² s⁻¹] were 383.00±18.40 and 12.95±0.42 for wild C. nitidissima (in mid-July) and 691.00±47.39 and 21.91±1.28 for wild C. sinensis, respectively. C. nitidissima is a shade tolerant species, whereas C. sinensis has a wide ecological range of adaptability to irradiance. Both wild and cultivated C. nitidissima demonstrated low maximum net photosynthetic rate, maximum carboxylation rate, maximum electron transfer rate, and SI, which indicated low photosynthesis ability of leaves that were unable to adapt to strong irradiance environment. Both C. nitidissima and C. sinensis demonstrated strong photosynthetic adaptabilty in new environments. Hence proper shading may raise photosynthetic efficiency of cultivated C. nitidissima and promote its growth.     

Changes in leaf photosynthesis of transgenic rice with silenced <Emphasis Type="Italic">OsBP-73</Emphasis> gene

In comparison with its wild type (WT), the transgenic (TG) rice with silenced OsBP-73 gene had significantly lower plant height, grain number per panicle, and leaf net photosynthetic rate (P _N). Also, the TG rice showed significantly lower chlorophyll (Chl), ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO), RuBPCO activase, and RuBP contents, photosystem 2 (PS2) photochemical efficiency (F_v/F_m and ΔF/F_m′), apparent quantum yield of carbon assimilation (Φ_c), carboxylation efficiency (CE), photosynthetic electron transport and photophosphorylation rates as well as sucrose phosphate synthase activity, but higher intercellular CO₂ concentration, sucrose, fructose, and glycerate 3-phosphate contents, and non-photochemical quenching of Chl fluorescence (NPQ). Thus the decreased P _N in the TG rice leaves is related to both RuBP carboxylation and RuBP regeneration limitations, and the latter is a predominant limitation to photosynthesis.     

Acclimation of photosynthesis to temperature in <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> and <Emphasis Type="Italic">Brassica oleracea</Emphasis>

Plants differ in how much the response of net photosynthetic rate (P _N) to temperature (T) changes with the T during leaf development, and also in the biochemical basis of such changes in response. The amount of photosynthetic acclimation to T and the components of the photosynthetic system involved were compared in Arabidopsis thaliana and Brassica oleracea to determine how well A. thaliana might serve as a model organism to study the process of photosynthetic acclimation to T. Responses of single-leaf gas exchange and chlorophyll fluorescence to CO₂ concentration measured over the range of 10–35 °C for both species grown at 15, 21, and 27 °C were used to determine the T dependencies of maximum rates of carboxylation (V_Cmax), photosynthetic electron transport (J_max), triose phosphate utilization rate (TPU), and mesophyll conductance to carbon dioxide (g’_m). In A. thaliana, the optimum T of P _N at air concentrations of CO₂ was unaffected by this range of growth T, and the T dependencies of V_Cmax, J_max, and g’_m were also unaffected by growth T. There was no evidence of TPU limitation of P _N in this species over the range of measurement conditions. In contrast, the optimum T of P _N increased with growth T in B. oleracea, and the T dependencies of V_Cmax, J_max, and g’_m, as well as the T at which TPU limited P _N all varied significantly with growth T. Thus B. oleracea had much a larger capacity to acclimate photosynthetically to moderate T than did A. thaliana.     

Gas exchange and water relations of three <Emphasis Type="Italic">Vitis vinifera</Emphasis> L. cultivars growing under Mediterranean climate

Optical characteristics, contents of photosynthetic pigments, total soluble sugars, and starch, rates of gas exchange, chlorophyll (Chl) a fluorescence, and leaf water relations were analysed in three Vitis vinifera L. cultivars, Tinto Cão (TC), Touriga Nacional (TN), and Tinta Roriz (TR), grown in Mediterranean climate. Chl content was significantly lower in TC than in TN and TR leaves, while the Chl a/b ratio was higher. TR had the lowest net photosynthetic rate, stomatal conductance, and contents of soluble sugars and starch than TN and TC. In spite of low Chl content, TC showed the lowest photon absorbance and the highest photochemical efficiency of photosystem 2. TC had the lowest predawn and midday leaf water potential. The capability for osmotic adjustment was similar among cultivars and the calculated modulus of elasticity was higher in TC leaves. The typical lighter green leaves of TC seemed to be an adaptive strategy to high irradiance and air temperature associated to water stress.     

Co<Subscript>2</Subscript> assimilation and chlorophyll fluorescence in green <Emphasis Type="Italic">versus</Emphasis> red <Emphasis Type="Italic">Berberis thunbergii</Emphasis> leaves measured with different quality irradiation

Photosynthesis, photorespiration, and chlorophyll (Chl) fluorescence in green and red Berberis thunbergii leaves were studied with two different measuring radiations, red (RR) and “white” (WR). The photosynthetic and photorespiration rates responded differently to the different radiation qualities, which indicate that the carboxylase and oxygenase activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) were affected. Differences in photosynthetic rate between the two color leaves were less under RR than under WR. However, this reduced difference in photosynthetic rate was not correlated with the stomatal response to the measuring radiation qualities. Compared with the WR, the RR reduced the differences in dark-adapted minimum and maximum fluorescence, steady-state fluorescence, light-adapted maximum fluorescence, and actual photochemical efficiency (Φ_PS2) of photosystem 2 (PS2), but enlarged the difference in non-photochemical quenching between the two color leaves. Differences in both maximum quantum yield of PS2 and ratio of Φ_PS2 to quantum yield of CO₂ fixation between the two color leaves were similar under the two measuring radiations. To exclude disturbance of radiation attenuation caused by anthocyanins, it is better to use RR to compare the photosynthesis and Chl fluorescence in green versus red leaves.     

Gas exchange characteristics and water relations in two cultivars of <Emphasis Type="Italic">Hibiscus esculentus</Emphasis> under waterlogging

Thirty-day-old plants of two okra cultivars, Sabzpari and Chinese-red, were subjected for 30 d to normal watering or continuous flooding. Continuous flooding did not cause any adverse effect on shoot fresh and dry biomass. Leaf water potential and pressure potential of both cultivars increased significantly due to waterlogging, but there was a slight increase in leaf osmotic potential. Chlorophyll a and b contents decreased significantly and chlorophyll a/b ratio increased. Waterlogging caused a significant reduction in net photosynthetic rate, water use efficiency and intrinsic water use efficiency, but stomatal conductance and intercellular CO₂/ambient CO₂ ratio remained unchanged.     

The impact of <Emphasis Type="Italic">trans</Emphasis>-zeatin <Emphasis Type="Italic">O</Emphasis>-glucosyltransferase gene over-expression in tobacco on pigment content and gas exchange

The responses of tobacco plants over-expressing trans-zeatin O-glucosyltransferase gene under constitutive or senescence-inducible promoter (35S:ZOG1 and SAG12:ZOG1) and of wild type (WT) plants to water stress and subsequent rehydration were compared. In plants sufficiently supplied with water, both transgenics have higher net photosynthetic rate (P_N) in upper and middle leaves and higher stomatal conductance (g_s) in middle leaves than WT. Water use efficiency (WUE = P_N/E) was higher in both transgenics than in WT. During prolonged water stress, both P_N and E declined to a similar extent in both transgenics and WT plants. However, 7 d after rehydration P_N in SAG:ZOG (upper and middle leaves) and 35S:ZOG (upper leaves) was higher than that in WT plants. Increased content of endogenous CKs in 35S:ZOG plants did not prevent their response to ABA application and the results obtained did not support concept of CK antagonism of ABA-induced stomatal closure. The chlorophyll (Chl) a+b content was mostly higher in both transgenics than in WT. During water stress and subsequent rehydration it remained unchanged in upper leaves, decreased slightly in middle leaves only of WT, while rapidly in lower leaves. Total degradation of Chl, carotenoids and xanthophyll cycle pigments (XCP) was found under severe water stress in lower leaves. Carotenoid and XCP contents in middle and upper leaves mostly increased during development of water stress and decreased after rehydration. While β-carotene content was mostly higher in WT, neoxanthin content was higher in transgenics especially in 35S:ZOG under severe stress and after rehydration. The higher content of XCP and degree of their deepoxidation were usually found in upper and middle leaves than in lower leaves with exception of SAG:ZOG plants during mild water stress.     

Crassulacean acid metabolism in the epiphytic fern <Emphasis Type="Italic">Patycerium bifurcatum</Emphasis>

The epiphytic fern Platycerium bifurcatum grows in different habitats characterized by drought and high irradiance stress. The plant shows diurnal malate oscillations, indicative for CAM expression only in cover leaves, but not in sporotrophophyll. In P. bifurcatum cover leaves exposed to high irradiance and desiccation, the decrease in both CO₂ assimilation (P _N) and stomatal conductance (g _s) was accompanied with occurrence of diurnal malate oscillations. Exogenously applied abscisic acid (ABA) induced the decrease in P _N and g _s, but no clear change in malate oscillations. The measurements of the maximum quantum efficiency of photosystem 2 (F_v/F_m) under high irradiance showed distinct photoinhibition, but no clear changes in F_v/F_m due to desiccation and ABA-treatment were found.     

Changes in effects of ozone exposure on growth,photosynthesis, and respiration of <Emphasis Type="Italic">Ginkgo biloba</Emphasis> in Shenyang urban area

An open-top chamber experiment was carried out from April through October 2006 to examine the effects of elevated (80 nmol mol⁻¹) atmospheric O₃ on Ginkgo biloba (4-years-old) in urban area. The air with ambient O₃ (AA, ≈ 45 nmol mol⁻¹) was used as control. The leaf mass and size, leaf area index, net photosynthetic rate (P _N), apparent quantum yield, transpiration rate, and stomatal conductance were decreased by elevated O₃ (EO) exposure. Visible foliar injury, which is light-brown flecks, was observed in the EO OTCs after 90 d of exposure. Carboxylation efficiency (Φ_CO2) and photorespiration and dark respiration rates were enhanced by EO exposure in the first half of the season, but all of them turned to be lower than those of the AA control at the end of experiment. Stomata limitation of photosynthesis was significantly higher than control in the whole season (p<0.05). Chlorophyll (Chl) content was lower in EO variant than in the control and the difference became more and more apparent through the season. Hence the decrease in P _N of G. biloba exposed to EO was the result of both stomatal and non-stomatal limitations. In the early season, the inhibition of photosynthesis was mainly caused by the stomatal limitation, and the earliest response was photoprotective down-regulation of photosynthesis but not photodamage. However, at the end of the season, the non-stomatal limiting factors such as decrease in Chl content, decrease in Φ_CO2, and anti-oxidative enzyme activity became more important.     

Role of changing environmental parameters in leaf gas exchange of <Emphasis Type="Italic">Arbutus unedo</Emphasis> L. assessed by field and laboratory measurements

In the frame of the foreseen climate global changes we analysed the physiological responses of Arbutus unedo L. to the variations of carbon dioxide concentration, leaf temperature, and irradiance by measurements of leaf gas exchange and leaf water potential performed both in field and in the laboratory. Stomatal conductance was not affected by increase of leaf temperature. The growth conditions of potted plants likely made stomata more sensitive to the variation of external parameters than naturally growing plants. The interaction between high CO₂ concentration and temperature involved important down-regulation mechanisms in the metabolic pathway of the carbon fixation. From an ecological point of view, the ability of A. unedo to adapt to the field stress makes it highly competitive in the Mediterranean plant community.     

Photosynthetic characteristics of hybrid rice with phospho<Emphasis Type="Italic">enol</Emphasis>pyruvate carboxylase gene

High level of phosphoenolpyruvate carboxylase (PEPC) gene was stably inherited and transferred from the male parent, PEPC transgenic rice, into a female parent, japonica rice cv. 9516. Relative to the female parent, the produced JAAS45 pollen lines exhibited high PEPC activity (17-fold increase) and also higher photosynthetic rates (about 36 %-fold increase). The JAAS45 pollen lines were more tolerant to photoinhibition and to photo-oxidative stress. Furthermore, JAAS45 pollen lines, as well as their male parent, were tested to exhibit a limiting C₄ cycle by feeding with exogenous C₄ primary products such as oxaloacetate (OAA). Thus the PEPC gene and photosynthetic characteristics of PEPC transgenic rice could be stably transferred to the hybrid progenies, which might open a new breeding approach to the integration of conventional hybridization and biological technology. An erratum to this article is available at .     

Photoautotrophic culture conditions and photosynthetic photon flux influence growth of <Emphasis Type="Italic">Lilium</Emphasis> bulblets <Emphasis Type="Italic">in vitro</Emphasis>

Summary Lilium Asiatic hybrid ‘Mona’ bulblets were cultured in vitro for 100 d under photoautotrophic (CO₂-enriched conditions and without sucrose in the medium) and heterotrophic (non-enriched CO₂ conditions and sucrose-supplemented medium) methods and under various levels of photosynthetic photon flux (PPF). Bulblet growth and net photosynthetic rate (NPR) were analyzed. CO₂₋ and PPF-enriched conditions enhanced the overall growth of bulblets, scale leaves, and roots. Heterotrophic conditions enhanced bulblet growth but higher PPF levels were inhibitory to the development of scale leaves. These results indicate the CO₂₋ and PPF-enriched conditions (photoautotrophic conditions) are beneficial for the production of high-quality bulblets of Asiatic hybrid lilies in vitro     

Ca<Superscript>2+</Superscript> reduces the effect of hypoxia in mosses <Emphasis Type="Italic">Mnium undulatum</Emphasis> and <Emphasis Type="Italic">Polytrichum commune</Emphasis>

Gametophores of mosses Mnium undulatum and Polytrichum commune were submerged in distilled water or in calcium chloride solution (0.9 mM Ca²⁺) to induce hypoxia. The net photosynthetic (P_N) and dark respiration rate (R_D) were measured in the air containing 300–400 μmol(CO₂)·mol⁻¹(air) and 0.21 mol(O₂)·mol⁻¹(air). P_N of M. undulatum gametophores decreased to 58 % of the control after 1-h submersion in water, whereas to 80 % of the control in P. commune gametophores. A smaller decrease in P_N was observed when the gametophores were immersed in CaCl₂ solution. In hypoxia, R_D in the tested mosses species was a little higher than in the control.     

Effects of <Emphasis Type="Italic">in vitro</Emphasis>rooting environments and irradiance on growth and photosynthesis of strawberry plantlets during acclimatization

Strawberry (Fragaria ananassaDuch. cv. Fengxiang) plantlets were cultured under two in vitroenvironments for rooting, and then acclimatized under two ex vitroirradiance conditions. At the end of rooting stage plant height, fresh weight and specific leaf area of T1-plants grown under high sucrose concentration (3 sucrose), low photosynthetic photon flux density (30 mol m^–2 s^–1) and normal CO₂ concentration (350–400 l l^–1) were significantly higher than those of T2-plantlets grown under low sucrose concentration (0.5), high photosynthetic photon flux density (90 mol m^–2 s^–1) and elevated CO₂ concentration (700–800 l l^–1). But T2-plantlets had higher net photosynthetic rate (Pn), effective photochemical quantum yield of PSII (_PSII), effective photosynthetic electron transport rate (ETR), photochemical quenching (q_P) and ratio of chlorophyll fluorescence yield decrease (Rfd). After transfer, higher irradiance obviously promoted the growth of plantlets and was beneficial for the development of photosynthetic functions during acclimatization. T2-plantlets had higher fresh weight, leaf area, _PSII and ETR under higher ex vitroirradiance condition.     

Effect of doubled CO<Subscript>2</Subscript> on morphology: Inhibition of stomata development in growing birch (<Emphasis Type="Italic">Betula platyphylla</Emphasis> Suk.) leaves

Two-year old birch (Betula platyphylla Suk.) seedlings were grown in climatic chambers for 7 weeks under various conditions: (1) ambient CO₂ concentration (350 ppm) and an ordinary nitrogen content in soil (2 mM NH₄NO₃); (2) ambient CO₂ concentration and a high nitrogen rate (16 mM NH₄NO₃); (3) doubled CO₂ concentration (700 ppm) and ordinary nitrogen content, and (4) doubled CO₂ concentration and a high nitrogen rate. Doubled CO₂ concentration in combination with the high nitrogen rate activated mostly seedling growth, e.g., stem thickening and leaf initiation. In this treatment, the maximum rate of apparent photosynthesis (A _max) was twice as high as in control seedlings. At doubled CO₂ concentration and ordinary nitrogen content, we observed the phenomenon of stomata absence from the upper leaf surface and doubling their number on the lower surface, whereas, at doubled CO₂ concentration and a high nitrogen rate, stomata partition was essentially similar as in control leaves. The conclusion is that, when the balance between CO₂ concentration and nitrogen rate is shifted, doubled CO₂ concentration exerts a morphotropic effect on differentiation of young epidermal tissue.Translated from Fiziologiya Rastenii, Vol. 52, No. 2, 2005, pp. 198–202.Original Russian Text Copyright © 2005 by Mao, Y.-J. Wang, X.-W. Wang, Voronin.This revised version was published online in April 2005 with a corrected cover date.