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肝炎病毒与EB病毒重叠感染 总被引:2,自引:0,他引:2
为探讨肝炎病毒(HV)与EB病毒(EBV)重叠感染的状况和后果,我们用免疫酶法对154例各型病毒性肝炎患者作了EBVIgA抗体检测。结果发现,急性肝炎、慢性轻度肝炎、慢性中度肝炎、肝炎肝硬化、慢性重型肝炎和原发性肝癌VGA-IgA抗体的阳性率分别为24.0%、30.0%、53.3%、63.3%、40.0%和72.7%,与健康人(5.3%)比较,有非常显著升高(P<0.01);原发性肝癌又较急性肝炎和慢性轻度肝炎高,并有非常显著意义差异(P<0.01)。HBV和HAV+HBV感染者比较,前者又较后者低(P<0.01)。重叠感染者的临床表现均为“肝炎型”,未见咽炎、腺热、胃肠、肺炎、肾炎、神经等类型。重叠感染者的CD+3及CD+4T细胞下降,CD+8T细胞及IgG,IgM升高,与健康人比较差异非常显著意义(P<0.01)。结果提示:HV感染,不仅因免疫失调易感EBV,又可因重叠感染而进一步使免疫功能失调;对病毒性肝炎的处理应强调免疫调节治疗。 相似文献
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浙江大蒜病毒的毒源鉴定 总被引:2,自引:0,他引:2
陆关成 《Virologica Sinica》1992,7(2):197-206
浙江余姚白蒜等S个品种均有病毒病,发病率100%,呈现黄条褪绿、花叶和畸形扭曲。人工接种蚕豆(Viciafaba)产生局部褐斑,墙生藜(Chenopodium murabe)上呈现褪绿点斑或隐症,豇豆(Vigna sinensis)上为坏死点斑或花叶。电镜检测病毒粒子为线形,长度为300—2250×11—12nm,其中550—650nm占29.6%、700—800hm占28%;超薄切片发现大量风轮状、环状、束状和涡轮状胞质内含体,未见片层状聚集体,叶绿体被破坏。致死温度在55℃以上。免疫电镜测试和洋葱黄矮病毒(OYDV)有血清学相关性,根据以上特征证明浙江大蒜病毒病是由两种病毒复合感染引起的,一种是大蒜黄条病毒(GYSV),属马铃薯Y病毒群(Potyviruses);另一种是大蒜隐潜病毒(GLV),属于石竹隐潜病毒群(Carlaviruses)。 相似文献
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Virus Replication in Enucleate Cells: Vesicular Stomatitis Virus and Influenza Virus 总被引:28,自引:17,他引:11
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The requirement of the presence of a nucleus for the replication of vesicular stomatitis virus and influenza virus has been examined by following the growth and development of these viruses in enucleate BS-C-1 cells. Vesicular stomatitis virus replicates normally in enucleate cells with the rate of production of infectious virus, the amount of virus-specific protein synthesis, and the type of proteins produced being essentially the same in nucleate and enucleate cells. Influenza virus does not replicate in enucleate cells, no virus gene products can be detected, and there is no inhibition of cellular protein synthesis. 相似文献
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Treatment of suspensions of vesicular stomatitis virus with Tween-ether results in a rapid and considerable loss of infectivity (ca. 4 logs in 2 min), but the residual infectivity is comparatively stable to further treatment with ether. The infectivity remaining after the short exposure to Tween-ether is not due to virus for the following reasons. (i) It is much less infective for tissue cultures than for mice, whereas the intact virion is equally infective for both hosts. (ii) The residual infectivity is much less stable than virus infectivity in both sucrose and tartrate gradients. (iii) Virus immune serum does not neutralize its activity. (iv) The infectivity is associated with material which sediments further in sucrose gradients and has a greater buoyant density in tartrate gradients than the virion. Experiments with (32)P-labeled virion showed that the infective substructure contains ribonucleic acid with the same sedimentation characteristics as that extracted from the virion. Electron microscopy shows that the infective component has the same overall bullet-like structure as the virion but lacks the outer envelope and fringe structure. 相似文献
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Mutations of Bacteria from Virus Sensitivity to Virus Resistance 总被引:307,自引:0,他引:307
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Donald M. McLean Cathron Cobb Susan E. Gooderham Carol A. Smart A. G. Wilson W. E. Wilson 《CMAJ》1967,96(11):660-664
Powassan virus isolations were achieved from three of 60 pools of Ixodes cookei ticks removed from 286 groundhogs (Marmota monax) which were collected some 200 miles north of Toronto between May 5 and September 5, 1966. Virus yields per pool of one to 11 ticks ranged from 102.5 to 106.0 TCD50 for primary swine kidney tissue cultures, and positive pools were collected on June 24, July 15 and August 10. Powassan neutralizing antibodies were detected by mouse inoculation tests in 143 of 362 animals including 127 of 286 groundhogs, 14 of 45 red squirrels (Tamiasciurus hudsonicus) and two of 31 other forest mammals. The monthly prevalence of antibody in the current season''s groundhogs increased from 0 to 25% with the progression of summer, but in older animals the incidence remained between 38 and 62% throughout the season. These results substantiate earlier findings which pointed towards the maintenance of Powassan virus in nature by a cycle involving groundhogs and squirrels as reservoirs, with ticks as vectors, from which human infections occurred tangentially. 相似文献
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Nucleocapsid Protein Subunits of Simian Virus 5, Newcastle Disease Virus, and Sendai Virus 总被引:30,自引:24,他引:6
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Walter E. Mountcastle Richard W. Compans Lawrence A. Caliguiri Purnell W. Choppin 《Journal of virology》1970,6(5):677-684
Helical nucleocapsids of each of the paramyxoviruses simian virus 5 (SV5), Newcastle disease virus (NDV), and Sendai virus have been isolated in two different forms. One form contains larger protein subunits and is obtained from mature virions or infected cells dispersed by ethylenediaminetetraacetic acid. The other form possesses smaller subunits and is obtained from infected cells dispersed by trypsin. The estimated molecular weights of the larger subunits in the three viruses are similar: SV5, 61,000; Sendai virus, 60,000; NDV, 56,000. The smaller nucleocapsid subunits are also very similar: SV5, 43,000; Sendai virus, 46,000; NDV, 47,000. The helical nucleocapsid composed of the smaller subunit appears to be less flexible and more stable than that formed by the larger subunit. There is suggestive evidence that conversion of the larger subunit to the smaller by proteolytic cleavage may occur intracellularly. The possibility that such a mechanism could be involved in the accumulation of nucleocapsid in cells persistently infected with paramyxoviruses is discussed. 相似文献
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收集236例胃癌组织标本及135份健康人群咽漱液(throat washings,TWs)标本,采用原位杂交、PCR-Southern blot筛选出17例EB病毒相关胃癌(EBVaGC)(7.2%)和33例EBV阳性的TWs标本(24.4%);应用PCR-RFLP、巢式PCR及DNA测序等方法,检测EBV阳性标本病毒1/2分型、F/f分型、I/i分型及LMP1XhoI(+)/(-)等四种基因变异。EBVaGC及健康对照均为F型变异,未检测到f型变异。EBVaGC中1/2型、I/i型及LMP1XhoI(+)/(-)型的例数及比例分别为:17(100%)/0(0)、1(5.9%)/16(94.1%)及0(0)/15(88.2%);而TWs中上述分型的相应数据为25(75.8%)/8(24.2%)、11(33.3%)/19(57.6%)及12(36.4%)/18(54.5%),各位点两种基因型在EBVaGC和健康人中的分布不同(1/2:P=0.047;I/i:P=0.048;XhoI(+)/(-):P=0.012)。综合分析表明在3种基因多态性均能确定的标本中,EBVaGC均为1/i/XhoI(-)亚型(15/1... 相似文献
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Parainfluenza Virus 5 Expressing the G Protein of Rabies Virus Protects Mice after Rabies Virus Infection 总被引:1,自引:0,他引:1
Rabies remains a major public health threat around the world. Once symptoms appear, there is no effective treatment to prevent death. In this work, we tested a recombinant parainfluenza virus 5 (PIV5) strain expressing the glycoprotein (G) of rabies (PIV5-G) as a therapy for rabies virus infection: we have found that PIV5-G protected mice as late as 6 days after rabies virus infection. PIV5-G is a promising vaccine for prevention and treatment of rabies virus infection. 相似文献
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Hepatitis C virus is a blood-borne virus that typically establishes a chronic infection in the liver, which often results in cirrhosis and hepatocellular carcinoma. Progress in understanding the complete virus life cycle has been greatly enhanced by the recent availability of a tissue culture system that produces infectious virus progeny. Thus, it is now possible to gain insight into the roles played by viral components in assembly and egress and the cellular pathways that contribute to virion formation. This minireview describes the key determining viral and host factors that are needed to produce infectious virus. 相似文献
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