Bullera koratensis sp. nov. and Bullera lagerstroemiae sp. nov., two new ballistoconidium-forming yeast species in the Trichosporonales clade isolated from plant leaves in Thailand

Seven strains of ballistoconidiogenous yeasts that contain xylose, form Q-10 ubiquinone, propagate by budding and don't produce stalk conidia were isolated from plant leaves collected in Thailand and were found to represent two new species. The taxonomic properties of the two species coincided with the genus Bullera so they are described as Bullera koratensis sp. nov. and Bullera lagerstroemiae sp. nov. In phylogenetic trees based on the nucleotide sequences of 18S ribosomal DNA and the D1/D2 domain of 26S rDNA, these two species are located in the Trichosporonales clade (Cryptococcus humicola-Trichosporon lineage).     

Bullera taiwanensis sp nov and Bullera formosensis sp nov,two new ballistoconidium-forming yeast species isolated from plant leaves in Taiwan

Two strains of ballistoconidiogenous yeasts that contain xylose and form Q-10 ubiquinone were isolated from plant leaves collected in Taiwan and were found to represent two new species. The taxonomic properties of both coincide with the genus Bullera so they are described as Bullera taiwanensis sp. nov. and Bullera formosensis sp. nov. In a phylogenetic tree based on the nucleotide sequences of 18S ribosomal DNAs, these two species are distant from the clusters where the remaining members of the genus Bullera are located, i.e., Bullera taiwanensis is located in the Filobasidium lineage (Filobasidiales clade) and Bullera formosensis is located in the Cryptococcus humicola-Trichosporon lineage (Trichosporonales clade).     

Bullera anomala sp. nov. and Bullera pseudovariabilis sp. nov., two new ballistoconidium-forming yeast species from Yunnan,China

Two ballistoconidium-forming yeast strains, CH 2.504 and CH 2.509, were isolated from the wilting leaves of Parthenocissus sp. collected in Yunnan, China in 1996. These two strains were assigned to the genus Bullera Derx by the characteristics of forming rotationally symmetric ballistoconidia, containing xylose in the cell hydrolysates and having Q-10 as the major ubiquinone. Molecular phylogenetic analysis based on small subunit ribosomal RNA gene (18S rDNA) and internal transcribed spacer (ITS) region sequences revealed that CH 2.504 is closely related to B. variabilis Nakase & Suzuki, while CH 2.509 is located in a clearly separate clade among the described Bullera species and related taxa. The results indicated that CH 2.509 represents a new species, for which Bullera anomala sp. nov. is the proposed name (type strain AS 2.2094^T). Further DNA-DNA reassociation data showed that CH 2.504 also represents a distinct species, for which the name Bullera pseudovariabilis sp. nov. is given (type strain AS 2.2092^T). This revised version was published online in June 2006 with corrections to the Cover Date.     

Pichia nongkratonensis sp. nov., a new species of ascomycetous yeast isolated from insect frass collected in Thailand

A yeast strain isolated from insect frass collected in Thailand was found to represent a new species of the genus Pichia. It is described as Pichia nongkratonensis sp. nov. In the phylogenetic tree based on the D1/D2 domain sequences of 26S rDNA, this yeast constitutes a cluster with Pichia dryadoides with high bootstrap confidence level; however, it differs from the latter species by 5.6% base substitutions. Pichia nongkaratonensis resembles P. dryadoides also in the phenotypic characteristics but is distinguished from this species by the assimilation of several carbon and nitrogen compounds.     

Citeromyces cibodasensis sp. nov., a novel yeast species isolated from leaf litter in Indonesia

A novel yeast species was isolated from leaf litter of Macropanax dispermus obtained from the Cibodas Botanical Garden, West Java, Indonesia. The two strains of the species displayed typical characteristics of the genus Citeromyces. Phylogenetic analysis based on the gene sequences of the D1/D2 domains of large subunit (LSU) rDNA, internal transcribed spacer (ITS) including 5.8S rDNA, mitochondrial small-subunit rRNA gene (MtSm), and translation elongation factor-1α (EF-1α) showed that the novel strains were clearly separated from the other four existing species of the genus Citeromyces. Therefore, the two strains were proposed to represent a novel species within the genus Citeromyces, for which the name Citeromyces cibodasensis is proposed; the type strain is NBRC 110244^T (= CBS 14272^T?=?InaCCY703^T?=?AK 01).     

Bensingtonia pseudonaganoensis sp. nov., a novel ballistoconidium-forming yeast species isolated from plant leaves

Among the basidiomycetous yeasts isolated from plant leaves collected in different regions of China, two ballistoconidium-forming strains were revealed to represent an undescribed species of the genus Bensingtonia by conventional, chemotaxonomic and molecular phylogenetic characterization. Sequence analysis of the 26S rDNA D1/D2 domains and the internal transcribed spacer (ITS) region indicated that the novel species was located in the Agaricostilbum lineage and closely related to Bensingtonia naganoensis and Bensingtonia ciliata, with the former as its closest relative. The name Bensingtonia pseudonaganoensis sp. nov. is proposed (type strain: AS 2.2601^T = CBS 10121^T)     

Rhodococcus cercidiphylli sp. nov., a new endophytic actinobacterium isolated from a Cercidiphyllum japonicum leaf

An endophytic actinobacterium, designated YIM 65003(T), was isolated from a surface sterilized leaf sample of Cercidiphyllum japonicum collected from Yunnan province, south-west China. The morphological and chemotaxonomic properties of the isolate were typical of members of the genus Rhodococcus. Analysis of the 16S rRNA gene sequence revealed that the isolate was most closely related to Rhodococcus fascians DSM 20669(T) (99.6%) and Rhodococcus yunnanensis YIM 70056(T) (99.0%). DNA-DNA hybridization with the above microorganisms (46.3% and 48.8%, respectively), in combination with differences in the biochemical and physiological properties, suggested that strain YIM 65003(T) should be classified within a novel species of the genus Rhodococcus, for which the name Rhodococcus cercidiphylli sp. nov. is proposed, with YIM 65003(T) (=CCTCC AB 207160(T)=DSM 45141(T)) as the type strain. The 16S rRNA gene sequence of strain YIM 65003(T) has been deposited in GenBank under the accession number EU325542.     

Fellomyces horovitziae sp. nov., a new basidiomycetous yeast species isolated from aXenasmatella basidiocarp

A new species of the genusFellomyces, F. horovitziae, was isolated from aXenasmatella basidiocarp. It differs from other accepted species in its carbon assimilation pattern, mol% G+C and low DNA-DNA homology. The delimiting characters are discussed and a key to the genus is provided.     

Microbotryozyma collariae gen. nov., sp. nov., a basidiomycetous yeast isolated from a plant bug Collaria oleosa (Miridae)

Two strains of a basidiomycetous yeast were derived from an insect trypanosomatid culture isolated from the intestine of a plant bug, Collaria oleosa (Heteroptera: Miridae), collected in Costa Rica. The yeast did not form ballistoconidia but reproduced only by budding. Teliospores were not observed in individual and crossed cultures of each strain. Morphological and other taxonomic characteristics of the yeast were similar to those of the species in the polyphyletic genus Rhodotorula. However, molecular phylogeny inferred from the internal transcribed spacers and D1/D2 region of the large subunit rRNA gene showed that the strains represent a new species placed among the smut fungi in the family Ustilentylomataceae, which includes Aurantiosporium subnitens, Fulvisporium restifaciens, Ustilentyloma fluitans, and Rhodotorula hordea. Given the well distinguished phylogenetic position of this novel species within the Ustilentylomataceae, we propose Microbotryozyma collariae gen. nov., sp. nov. to accommodate the yeast isolated from C. oleosa, with strain American Type Culture Collection MYA-4666^T (= PRA303-1S = CBS 12537) designated as the type strain.     

Fellomyces mexicanus sp. nov., a new member of the yeast genus Fellomyces isolated from lichen Cryptothecia rubrocincta collected in Mexico

Two strains isolated from the lichen Cryptothecia rubrocincta collected in Mexico are described as new members of the sterigmatoconidia-producing genus Fellomyces. Based on the identical sequences of the D1/D2 regions of 26S rDNA, as well as the results of the cluster analysis of the AFLP fingerprints, the strains have been shown to be conspecific. The 26S rDNA-based phylogeny has indicated that Fellomyces mexicanus clusters within a clade including species such as four other Fellomyces isolates from lichens: F. chinensis, F. lichenicola, F. sichuanensis and F. thailandicus. F. mexicanus is characterised by the presence of xylose in the cell walls and CoQ10 in the mitochondrial membrane. The type culture is strain HB25=CBS8279.     

Debaryomyces mycophilus sp. nov., a siderophore-dependent yeast isolated from woodlice

Four strains of an ascogenous yeast were isolated from the guts of the woodlice species Armadillidium vulgare (Latreille). This yeast differed from all known yeasts by its inability to grow in culture without the presence of a metabolite produced by some common soil fungi such as Cladosporium cladosporioides, Aspergillus alliaceus, and Penicillium spp. Phylogenetic analysis based on 18S rDNA and 26S rDNA (domain D1/D2) sequences indicated that the yeast represents a new taxon in the genus Debaryomyces. The new species Debaryomyces mycophilus is thus proposed. It was, furthermore, shown that the fungal metabolite necessary for growth of D. mycophilus did not provide the yeast with carbon, nitrogen or vitamins. The active compound was partially purified and it was shown that it is a siderophore used by the yeast as a source of iron. The addition of ferrichrome or high concentrations of FeCl(3) to growth media replaced the obligate dependence on a fungal metabolite. Symbiosis among fungi, based on the availability and utilization of iron, is an aspect of mycology that has not previously been recognized. The addition of chelated iron to isolation media could lead to the discovery of many unknown yeasts and fungi.     

Candida nonsorbophila sp. nov., a new ascomycetous yeast species isolated in Thailand

Four yeast strains, RS42, SSK10, ST-520 and ST-521, isolated from water in a mangrove forest, bark of a tree and fruit of Ficus sp., respectively, were found to represent a hitherto undescribed anamorphic species. The four strains are related to Candida sinolaborantium in the D1/D2 domain of the large subunit rRNA gene, but differed by eight nucleotide substitutions and two indels, and for this reason are regarded as members of a separate species. Because ascospore formation was not detected, it is described as a new species of Candida, Candida nonsorbophila sp. nov. The type strain is RS42^T (BCC 25963^T=NBRC 103860^T=CBS 10862^T). This species is distinguished from C. sinolaborantium by the inability to assimilate l -sorbose, l -rhamnose and galactitol, and a higher maximum growth temperature.     

巧花兜兰——越南中部兰科一新种

在今年年初的一次访问云南南部的旅行中,笔者曾购得数苗小型的兜兰,据兰商称系采自越南中部,又叫越南黄花仔。此种兜兰的叶甚似小叶兜兰ＰａｐｈｉｏｐｅｄｉｌｕｍｂａｒｂｉｇｅｒｕｍＴａｎｇｅｔＷａｎｇ和海伦兜兰Ｐａｐｈ.ｈｅｌｅｎａｅＡｖｅｒｙａｎｏｖ,但花却有着明显的不同。小叶兜兰的花大,直径达5.5～7ｃｍ;合萼片狭窄,其宽度仅为中萼片的1/2～3/5。海伦兜兰花纯黄色;花瓣线形或宽线形。而此种兜兰的花直径3～3.5ｃｍ,呈淡黄绿色,唇瓣表面、合萼片中脉下半部和花瓣的中脉及中脉上方一侧均有枣红色晕;合萼片宽度为中萼片的4/5;…