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1.
T M Samsonova A K Akatov M L Khatenever 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1983,(11):13-16
More than 200 coagulase-positive strains of animal origin have been studied by means of Staphylococcus aureus typing phages, belonging to two international sets and intended for typing staphylococci isolated from large cattle and humans, and experimental "chicken" phage A 1591. Among S. aureus strains the cultures isolated from swine, cows, chickens, and belonging to biotypes B1, C1, B2, respectively, have been mostly (in 78.5-90.0% of cases) determined by phage typing. The strains belonging to one biotype have proved to be sensitive predominantly to the same phages. In this connection further differentiation of staphylococci within individual biotypes by means of the phages used in these experiments seems to be impracticable. S. intermedius strains have been found to be completely resistant to the above phages, which confirms that S. intermedius is rightly considered to be an independent species of coagulase-positive staphylococci. 相似文献
2.
Characteristics of Staphylococcus aureus associated with meat products and meat workers 总被引:2,自引:0,他引:2
B.K. Isigidi L.A. Devriese † C. Godard ‡ J. Van Hoof§ 《Letters in applied microbiology》1990,11(3):145-147
Staphylococcus aureus strains with similar biotype characteristics occur in meat products and in the anterior nares of workers in slaughterhouses and meat-processing plants. Such strains are rare in humans not in frequent contact with meats. The biotype is staphylokinase-negative, β-haemolysin-negative, bovine plasma coagulase-negative, crystal violet type A and Protein A-positive. Most strains of this biotype belong to phage group II or are not typable with the international set for typing Staph. aureus of human origin. 相似文献
3.
Characterization of coagulase-positive staphylococci isolated from free-living birds. 总被引:3,自引:0,他引:3
V Hájek J Balusek V Horák D Koukalová 《Journal of hygiene, epidemiology, microbiology, and immunology》1991,35(4):407-418
A total of 129 coagulase-positive staphylococcal strains were isolated from the nasopharyngeal region of free-living birds--81 (16%) from 501 birds of prey, 29 (25%) from 117 water birds, and 19 (2%) from 937 pheasants. Staphylococcus aureus and Staphylococcus intermedius were identified in 64 (79%) and 17 (21%) strains from birds of prey, in 1 (3%) and 28 (97%) strains from water birds, and in 4 (21%) and 15 (79%) strains from pheasants, respectively. Of the total number of the 69 S.aureus strains 45 (65%) could be biotyped. Biotype D prevailed in the strains from birds of prey. Among all the 60 S.intermedius strains 41 (68%) biotype 1 and 19 (32%) biotype 3 strains could be recognized. Biotype 1 predominated in strains from water birds and pheasants, while biotype 3 in strains from birds of prey. Resistance to antibiotics was recorded in 8 (12%) S.aureus and 5 (8%) S.intermedius strains only. Fifty-six (81%) of the 69 S.aureus strains could be typed with human phages, 30 (44%) with bovine and 41 (51%) with chicken phages. It is evident that the host range of Shimizu's (CH) phages involves not only S.aureus from chicken but also from any other birds. None of these strains was typable with canine phages. Out of the 60 S.intermedius strains 45 (75%) were lysed with Blouse and Meekins' canine phages. When human, bovine, and chicken phages were used, all the 60 strains were completely resistant. 相似文献
4.
Bruhn Kaj Farah Ibrahim O. Pedersen Erik Halgaard Christian 《Acta veterinaria Scandinavica》1988,29(3-4):303-310
One Hundred and ten alpha and/or delta-haemolytic isolates (collection 1), 50 beta haemolytic isolates (collection 2) from bovine mastitis, and 100 previously phage-typed alpha- and delta-haemolytic isolates (human collection) og Staphylococcus aureus (S. aureus) were tested and biotyped according to the scheme of Hajek & Marsalek (1971). Among collection 1 isolates, 85 (77.3 %) belonged to the human biotype A (human source). Twenty two (20 %) designated as non-allotted strains, possessed characteristics of both animal and human sources. The remaining 3 isolates (2.7 %) in this collection belonged to biotype C (animal source). All collection 2 isolates which were used as control strains for animal sources, belonged to biotype C. The human collection that contained 100 phage-typed haemolytic isolates (representing all human phage groups) were used as a control for the human source. Irrespective of their phage group, these strains predominantly produced alpha and/or delta haemolysins and belonged to the human biotype A. This study also recommended the use of a combined plasma crystal violet agar medium for the presumptive identification of S. aureus biotypes. 相似文献
5.
Potential of the polyvalent anti-Staphylococcus bacteriophage K for control of antibiotic-resistant staphylococci from hospitals 总被引:1,自引:0,他引:1
O'Flaherty S Ross RP Meaney W Fitzgerald GF Elbreki MF Coffey A 《Applied and environmental microbiology》2005,71(4):1836-1842
The increasing prevalence of antibiotic-resistant staphylococci has prompted the need for antibacterial controls other than antibiotics. In this study, a lytic bacteriophage (phage K) was assessed in vitro for its ability to inhibit emerging drug-resistant Staphylococcus aureus strains from hospitals and other species of Staphylococcus isolated from bovine infections. In in vitro inhibitory assays, phage K lysed a range of clinically isolated methicillin-resistant S. aureus (MRSA) strains, S. aureus with heterogeneous vancomycin resistance and vancomycin resistance, and teicoplanin-resistant strains. In these assays, 14 of the MRSA strains were initially only weakly sensitive to this phage. However, propagation of phage K on these less-sensitive strains resulted in all 14 being sensitive to the modified phages. The results enforce the principle that, while certain target bacteria may be relatively insensitive to lytic phage, this can be overcome by obtaining modified phage variants from passage of the phage through the insensitive strains. Model in situ hand wash studies using a phage-enriched wash solution resulted in a 100-fold reduction in staphylococcal numbers on human skin by comparison with numbers remaining after washing in phage-free solution. Infusion of the phage into a nonimmunogenic bismuth-based cream resulted in strong anti-Staphylococcus activity from the cream on plates and in broth. 相似文献
6.
Enterotoxigenicity of Staphylococcus aureus Cultures Isolated from Acute Cases of Bovine Mastitis 总被引:7,自引:3,他引:4
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To determine whether staphylococci causing bovine mastitis are potential causes of human intoxications, 142 cultures identified as etiological agents of acute cases and 18 cultures causing chronic cases of staphylococcal mastitis were obtained from investigators in the United States and Canada, examined microscopically, and tested for carbohydrate utilization, terminal pH, catalase, coagulase, egg yolk hydrolysis, gelatin hydrolysis, cytochrome oxidase, urease production, nitrate reduction, micrococcal nuclease, phage type, and enterotoxin production. Three cultures were not confirmed as Staphylococcus aureus. Of the 157 S. aureus cultures, 23 produced staphylococcal enterotoxins. Although a direct relationship between staphylococcal mastitis and outbreaks of staphylococcal food poisoning was not proved, results indicated that staphylococcal infections of the bovine mammary gland represent a significant reservoir of enterotoxigenic strains of S. aureus. 相似文献
7.
Staphylococci occur in donkeys more frequently than in other animals, and only from donkeys coagulase-negative staphylococci, characteristic of humans (S. hominis, S. capitis, S. cohnii), were isolated. Least frequently staphylococcal carrier state was registered in cats; in these animals only coagulase-negative strains were found to occur. From 30 donkeys coagulase-positive staphylococci belonging to 47 S. aureus strains were isolated. These strains differed from known ecological variants in their biological properties, thus suggesting the existence of S. aureus ecovar specific for donkeys. These strains did not coagulate human, bovine and ovine plasma, but coagulated rabbit plasma in 100% of cases and donkey plasma only in 53% of cases; at the same time they relatively often produced delta hemolysin, rarely phosphatase and hyaluronidase and never fibrinolysin. These strains were typed by KPC phages, mainly 116 and 117. 相似文献
8.
A total of 57 gram-positive, catalase-positive cocci, considered etiological agents of clinical and subclinical bovine mastitis, were tested for glucose and mannitol fermentation, coagulase and thermonuclease production, sensitivity to lysostaphin, gelatin hydrolysis, lysozyme, phosphatase and egg yolk factor production, hemolytic properties, antibiotic sensitivity, susceptibility to human and bovine phages, and enterotoxin production. All 57 strains were identified as staphylococci. A good correlation was found between 3+ and 4+ coagulase reactions, thermonuclease production, and high sensitivity to lysostaphin. Neither mannitol fermentation nor production of other enzymes appeared to be a specific property of bovine Staphylococcus aureus strains. beta- and delta-hemolysins were more frequently found than alpha-hemolysin. Nearly 40% of the strains were penicillin resistant. Strains were lysed by phage 42E from the human phage set more frequently than by phage 42D, whereas with the bovine set, strains were more sensitive to specific bovine phages. Three strains produced enterotoxin C, and one strain produced enterotoxin D. 相似文献
9.
Interactive Growth of Staphylococcus aureus Strains with a Poultry Skin Microflora in a Diffusion Apparatus 总被引:2,自引:2,他引:0
The growth of different biotypes of Staphylococcus aureus strains isolated from poultry was studied using the NBS Ecologen in which the interacting mixed culture was derived from the microfiora of hen skin and separated from the Staph. aureus culture by a membrane of 0.4 μm pore size. Inhibition of growth of the Staph. aureus cultures occurred with strains from each biotype. Marked inhibition of growth was always accompanied by the production of large numbers (>109 /ml) of plaque forming units (phage). In the mixed culture chamber poultry phage group C strains became the predominant Staph. aureus type. The phages produced by the mixed culture showed a wide spectrum of lytic activity for the propagating strains of the human, bovine and poultry phage sets. 相似文献
10.
el-Ghodban A Ghenghesh KS Marialigeti K Tawil A 《Archives de l'Institut Pasteur de Tunis》1999,76(1-4):23-25
Enterotoxin was detected in 22 (61.1%) of the 36 S. aureus strains isolated from clinical materials and in 3 (13%) of the 23 S. aureus strains from food samples (P < 0.05). On the basis of individual types of enterotoxin, staphylococcal enterotoxin A (SEA) was produced by 11.1%, SEB by 38.9% and SEC by 22.2% of SS. aureus strains from clinical material. Of the food S. aureus strains, SEC and SED produced by 8.7% and 4.3% respectively. Of the clinical and food S. aureus strains, 52.8% and 39.1%, respectively, were typeable by the 23 phages of International Phage Set. The majority of the typeable S. aureus strains from clinical and food sources belonged to group II being at 22.2% and 17.4% respectively. Furthermore, of the 14 SEB-producing S. aureus, 42.9% were of phage group II. In conclusion, the results obtained indicate that enterotoxin-producing S. aureus strains from clinical materials in Libya are not uncommon; however, certain foods appear not to be the source of such strains. Because of the low susceptibility to bacteriophages shown by S. aureus isolated in Libya, compared to reports from several countries, other methods of typing should be used in conjunction with phage typing in epidemiological investigations concerning this organism. 相似文献
11.
Rodney F. Smith Carol L. Bettge Sandra L. Dayton James H. Jorgensen 《Applied microbiology》1973,25(1):15-20
A one-year study on an endemic strain of Staphylococcus aureus phage type 84/85 in a children's burn unit is described. The endemic strain rapidly colonized the burns and nares of acute patients after admission but was not isolated from a patient on admission. Nonendemic strains of S. aureus found on some new patients were mostly non-phage typable and did not prevail in burns. The endemic strain was rarely isolated from the nares and skin of reconstructive patients or from the nares of hospital personnel. The endemic strain did colonize the oral cavity, normal skin, and intestinal tract of some acute patients. Endemic and nonendemic strains of S. aureus from the burned children were compared in their biochemical activities and antibiotic sensitivities to two groups of S. aureus from one other local and one Danish burns unit. The latter groups of strains represented different combinations of staphylococcal phage group III strains. Each of the four groups of strains differed in production of hemolysins, Tween 80 hydrolysis, egg yolk reaction, and proteolysis of casein and gelatin. All of the strains were uniformly sensitive to gentamicin, oxacillin, and cephalothin. Only 4 of 162 strains tested were methicillin resistant. The endemic S. aureus strains of phage type 84/85 were uniformly resistant to eight other antibiotics including lincomycin and clindamycin. The endemic strain was not the known cause of a clinically documented infection in a group of 82 acute patients studied. The possible role of S. aureus strains of phage group III in burn grafting problems is discussed. 相似文献
12.
13.
The results of the five-year study of S. aureus and P. aeruginosa associations isolated from the sputa of pulmonological patients are presented. The incidence rate of these bacteria in monocultures and associations is estimated. The results of the phage typing and serotyping of S. aureus and P. aeruginosa strains suggest that the formation of the associations of these organisms occurs mainly due to the tendency of P. aeruginosa hospital strains to associate with S. aureus cultures present in the patients. 相似文献
14.
RAPD-PCR analysis of Staphylococcus aureus strains isolated from bovine and human hosts 总被引:3,自引:0,他引:3
Reinoso E Bettera S Frigerio C DiRenzo M Calzolari A Bogni C 《Microbiological research》2004,159(3):245-255
Staphylococcus aureus is one of the most important pathogens in humans and animals. In this study eighty strains were analyzed by RAPD-PCR to assess the genetic relationship between S. aureus isolates from bovine and human hosts. Results were compared with those obtained by biotyping. Fifty-two percent of the S. aureus isolates belonged to a host specific biotype (human, bovine and poultry). Bovine and human ecovars were the most prevalent. Dendrogram obtained by RAPD results showed that all the isolates clustered into eleven groups (A-K) at a relative genetic similarity of less than 30% when analyzed with the three primers. Group A clustered 95% of the human host isolates and the remaining groups (B-K) clustered the bovine host isolates. Principal coordinate analysis also showed that the isolates could be arbitrarily divided into two groups, bovine and human, by the second coordinate. Only 9 isolates (11%) were not clustered into these groups. The genetic diversity among the S. aureus isolates from bovine hosts is relatively low compared to that of isolates from human hosts. There were no statistically significant differences among isolated from bovine and human hosts. This study shows that RAPD-PCR assayed with three primers can be successfully applied to assess the genetic relationship of S. aureus isolates from different hosts. 相似文献
15.
以金黄色葡萄球菌为宿主菌,从奶牛场废水样品中分离到两株烈性噬菌体,分别命名为phiSA_BS1和phiSA_BS2。电子显微镜观察显示其头部呈多面体对称,有伸缩性尾部。对这两株噬菌体的一步生长曲线、温度耐受性和pH耐受性进行研究,发现两者均裂解3株金黄色葡萄球菌,但不能裂解1株表皮葡萄球菌。进一步分析它们的全基因组序列,发现phiSA_BS1基因组大小为 142 978 bp,GC含量为 29.80%,预测到201个开放读码框(open reading frame,ORF),未预测到tRNA基因;phiSA_BS2基因组大小为 149 230 bp,GC含量为 29.61%,预测到210个ORF和1个tRNA基因。系统发育分析表明,这两株噬菌体同属肌尾噬菌体科,属于新的金黄色葡萄球菌噬菌体,可能为治疗金黄色葡萄球菌相关的奶牛乳腺炎提供新的方法。 相似文献
16.
Piechowicz L Galiński J Dajnowska-Stanczewa A Garbacz K 《Medycyna do?wiadczalna i mikrobiologia》2005,57(2):105-113
The aim of study was to determine how population of Staphylococcus aureus strains from hospital environment forms itself with respect to phage types and frequency of their occurrence recently. 1157 S. aureus isolated from various clinical materials between 1999 and 2004 have been investigated. The basic set of phages and the three additional ones were used for typing strains according to Blair and Williams method. The results proved that population of S. aureus strains isolated from hospital environment differentiates with respect to phage types, like in the past. Strains belonging to phage group II dominates among them, but a considerable increase in number of phage group III have been remarked lately. A differential value of an individual phages from basic set remains on their usual level for years. 相似文献
17.
AIMS: To understand the potential use of bacteriophage K to treat bovine Staphylococcus aureus mastitis, we studied the role of whey proteins in the inhibition of the phage-pathogen interaction in vitro. METHODS AND RESULTS: The interaction of bacteriophage K and S. aureus strain Newbould 305 was studied in raw bovine whey and serum. Incubation of S. aureus with phage in whey showed that the bacteria are more resistant to phage lysis when grown in whey and also bovine serum. Whey collected from 23 animals showed a wide variation in the level of phage-binding inhibition. The role of the protein component of milk whey in this inhibition was established; treatment of the whey by heat, proteases and ultrafiltration removed the inhibitory activity. Brief exposure of S. aureus cells to whey, followed by resuspension in broth, also reduced phage binding. Microscopy showed the adhesion of extracellular material to the S. aureus cell surface following exposure to whey. Chromatographic fractionation of the whey demonstrated that the inhibitory proteins were present in the high molecular weight fraction. CONCLUSIONS: The adsorption of whey proteins to the S. aureus cell surface appeared to inhibit phage attachment and thereby hindered lysis. The inhibitory whey proteins are of high molecular weight in their native form and may sterically block phage attachment sites on the cell surface. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings have implications for any future use of phage therapy in the treatment of mastitis, and other diseases, caused by S. aureus. This pathogen is predicted to be much more resistant to phage treatment in vivo than would be expected from in vitro broth culture experiments. 相似文献
18.
Abstract In order to check the relationship between capsular polysaccharide (CP) type 5 and 8 and certain phage patterns, previously described in human Staphylococcus aureus clinical isolates, we typed 100 CP types 5 and 8 S. aureus strains isolated from cow, goat and ewe milk, with the human set of phages. The proportion of typable strains was much less than that found with human strains. The association between CP types 5 and 8 and phage patterns reported for human isolates was only partially confirmed and an original correlation between susceptibility to group III phages and CP type 5 was found. 相似文献
19.
Staphylococcus aureus strains 7-8 and 57 that produce beta-hemolysin but not staphylokinase (beta + K-) were lysogenically converted by certain serological group F bacteriophages to the loss of beta-hemolysin production and the gain in staphylokinase production (beta-K+). Serological group A phage 42E was found to convert S. aureus strains 7-8(beta-K-) and 57 (beta + K-) to beta - K-. Conversion of beta-hemolysin by lysogenization of a serological group A phage has not previously been reported. Phage 42E conversions differed from the group F conversions since staphylokinase was not affected. This indicates that conversion to beta-K+ involves separate loci on the phage chromosome. Several characteristics associated with virulence of staphylococci of human or animal origin other than staju;plomase production (coagulase, DNase, lipase, gelatinase, mannitol fermentation, and phage-sensitivity patterns) were not correlated with lysogenic conversions to loss of beta-hemolysin. 相似文献
20.
S Naidu P N Rao K Rajyalakshmi A S Naidu 《Journal of hygiene, epidemiology, microbiology, and immunology》1989,33(3):277-282
Out of the total 68 S. aureus strains isolated and studied from pyoderma patients, 33 (48.5%) strains produced enterotoxin. Isolates from IED, impetigo and folliculitis exhibited high degree of enterotoxigenicity. SE-C and its combinations with other enterotoxins was common. 60.6% of the SE producers were found phage nontypable. Typable enterotoxigenic strains were associated with III, IV and mixed phage groups. S. aureus var. hominis and S. aureus var. bovis are the prevalent subspecies types and potent SE producers among pyogenic skin infections. 相似文献