Influence of ATP and ADP on dissociation of the V-ATPase into its V(1) and V(O) complexes

Although the reversible dissociation of the V(1)V(O) holoenzyme into its V(1) and V(O) complexes is a general mechanism for the regulation of V-ATPases, important aspects are still not understood. By analyzing the endogenous nucleotide content of the V(1)V(O) holoenzyme and of the V(1) complex, both purified from Manduca sexta larval midgut, we found that the V(1) complex contained 1.7 molec. of ADP, whereas only 0.3 molec. of ADP were bound to the V(1)V(O) holoenzyme. By contrast, both proteins contained only negligible amounts of ATP. Incubation of the V(1)V(O) holoenzyme with various adenine nucleotides revealed that ATP hydrolysis, leading to a state containing tightly bound ADP is necessary for its dissociation.     

Reviews

The Principles of Insect Physiology. By V. B. WIGCLESWORTH.
The African Genera of Acridoidea. By V. M. DIRSH.
The Principles of Agricultural Entomology. By C. A. EDWARDS and G. W. HEATH.
Cereal Pests. By D. W. EMPSON. Pp. v + 97,8plates.
The Biology of Viruses. By KENNETH M. SMITH.
Radiotvacer Methodology in Biological Science. By C. H. WANG and DAVID L. WILLIS.
Tea, 2nd ed. By T. EDEN.     

Book Notices

Book reviewed in this article:
MITES OF MOTHS AND BUTTERFLIES. By Asher E. Treat.
THE SWALLOWTAIL BUTTERFLIES OF NORTH AMERICA. By Hamilton A. Tyler.
APHIDS. By Roger Blackman.
APHID PARASITES (HYMENOPTERA, APHIDIIDAE) OF THE MEDITERRANEAN AREA. By Petr Stary.
MAN'S PLAGUE. INSECTS AND AGRICULTURE. By V. G. Dethier.
STUDIES IN BIOLOGICAL CONTROL. Edited by V. L. Delucchi.     

Book notices

DISPERSAL CENTRES OF SPHINGIDAE (LEPIDOPTERA) IN THE NEOTROPICAL REGION. By Harald Schreiber.
TSETSE FLIES IN NIGERIA: A HANDBOOK FOR JUNIOR CONTROL STAFF. By Howell Davies.
INSECTS AND OTHER ARTHROPODS OF MEDICAL IMPORTANCE. By Kenneth G. V. Smith.
DISEASES AND PESTS OF ORNAMENTAL PLANTS, 5th edition. By Pascal P. Pirone.
NEW ZEALAND INSECT PESTS. Edited by D. N. Ferro.
THE LIFE OF THE BUMBLEBEE. By D. V. Alford.     

REVIEWS

Book reviewed in this article:
The I;undamcntCrls of FNit Production. By V. R. GARDNW, F. C. BRADFORD and H. D. HOOKER.
The Scientific Rim)les of PIat Protection with Special Rcfcence to Chemical C M. By HUBERT MARTIN.
Hanalbook of Plytopathogenic Viruses. By F. 0. HOLMES.
Plrmi V k e s rmd Vinu Diseases. By F. C. BAWDEN.     

Book notices

ENTOMOLOGY. By Cedric Gillott.
MEDICAL ZOOLOGY FOR TRAVELLERS. By John Hull Grundy.
RECENT ADVANCES IN ACAROLOGY. Edited by J. G. Rodriguez.
SAMPLING METHODS IN SOYBEAN ENTOMOLOGY. Edited by M. Kogan and D. C. Herzog.
MOUNTAIN TORRENT OF THE TIEN SHAN. A FAUNISTIC-ECOLOGY ESSAY. By K. A. Brodsky. Translated from Russian by V. V. Golosov.
ECOLOGY OF INSECT-PLANT INTERACTIONS. By Peter J. Edwards and Stephen D. Wratten.     

Book reviews

Books reviewed in this article:
SEXUALITY IN PLANTS AND ITS HORMONAL REGULATION. By M. Kh. C hailakhyan and V. N. K hrianin (edited by K enneth V. T himan ; translated by Vanya Loroch).
Tertiary Level Biology: PLANT GROWTH REGULATORS. By J. A. R oberts and R. H ooley
Tertiary Level Biology: PLANT MOLECULAR BIOLOGY. Second Edition. By D. G rierson and S. N. C ovey
SEVENTY-FIVE YEARS IN ECOLOGY: THE BRITISH ECOLOGICAL SOCIETY. By J ohn S heail     

活性氧胁迫促进枯草芽孢杆菌WSHDZ-01过量合成过氧化氢酶

研究了乙醇和H2O2胁迫对芽孢杆菌(Bacillussp.)WSHDZ-01过量合成过氧化氢酶(Catalase,简称CAT)的影响。在Bacillussp.WSHDZ-01培养体系中添加2.0%(V/V)乙醇,胞内过氧化氢酶酶活达到11151U/mL,是对照组的2.5倍。而在培养体系中添加0.3%(V/V)H2O2,则使胞内过氧化氢酶不断分泌到胞外,胞外酶占总酶活比率增加至27%。基于上述发现,分别以不添加胁迫物(a)、乙醇胁迫(b)、H2O2胁迫(c)为对照,在培养体系中维持持续的乙醇和H2O2胁迫,结果表明:1)胞外酶比例达到82.5%;2)发酵周期缩短为42h,比对照a延长了6h,比对照b和c分别缩短了8h和6h;3)生产强度达到470U/(mL·h),比对照a提高了18.6%,为过氧化氢酶工业化生产奠定了基础。     

REVIEWS

Books reviewed in this article:
Phytochemical Methods. By J. B. H arborne .
Annual Review of Phytopathology , Vol. 11. Ed. by K. F. B aker .
Bacterial and Fungal Diseases of Plants in the Tropics. By G. F. W eber .
Yeast Mould and Plant Protoplasts. Ed. by J. R. V illanueva , I. G arcía -A cha , S. G ascon and F. U ruburu .
Identification of the Larger Fungi. By R oy W atling .
An Atlas of Plant Structure. Vol. 2. By B. B racegirdle and P. H. M iles .
Taxonomy and Ecology. The Systematics Association: Special Volume No. 5. Ed. by V. H. H eywood .
Quantitative and Dynamic Plant Ecology. By K. A. K ershaw .
Models in Ecology. By J. M aynard S mith .
Vegetation of the Earth in Relation to Climate and the Eco-Physiological Conditions. By H. W alter .
A New Ecophysiological Approach to Forest Water Relationships in Arid Climates. By I. G indel .
Flore de France. Vol. 1. By M arcel G uinochet and R oger de V ilmorin .     

REVIEWS

Nature's Second Kingdom. By F. D elaporte . Translated by Arthur Goldhammer.
Encyclopedia of Plant Physiology , New Series, Volume 10. Hormonal regulation of development II. The functions of hormones from the level of the cell to the whole plant. Edited by T. K. S cott .
Cell Culture and Somatic Cell Genetics of Plants , Volume 1. Laboratory procedures and their applications. Edited by I. K. V asil .
Databases in Systematics. Edited by R. A llkin and F. A. B isby .
Plant Chemosystematics. By J. B. H arborne and B. L. T urner .
Current Concepts in Plant Taxonomy. Edited by V. H. H eywood and D. M. M oore .
Introduction to Principles of Plant Taxonomy. By V. V. S ivarajan .
Our Green and Living World: The Wisdom to Save It. By E. S. A yensu , V. H. H eywood , G.L. L ucas and R. A. D efillips .
Lipids in Plants and Microbes. By J. L. H arwood and N. J. R ussell .
The Role of Terrestrial Vegetation in the Global Carbon Cycle: Measurement by Remote Sensing. Edited by G. M. W oodwell .
Chromosomes Today , Volume 8. Edited by M. D. B ennett , A. G ropp and U. W olf .     

Book Notices

Book reviewed in this article:
ANTS. By M. V. Brian
THE LEPIDOPTERA OF NORFOLK ISLAND, THEIR BIOGEOGRAPHY AND ECOLOGY. Series Entoraologica 13. By J. D. Holloway
IMMS' GENERAL TEXTBOOK OF ENTOMOLOGY. Tenth Edition. By O. W. Richards and R. G. Davies
THEORY AND PRACTICE OF BIOLOGICAL CONTROL. Edited by C. B. Huffakerand (the late) P. S. Messenger
THE BRITISH BUTTERFLIES, THEIR ORIGIN AND ESTABLISHMENT. By R. L. H. Dennis
GEOGRAPHIC VARIATION, SPECIATION AND CLINES. By John A. Endler
MISSION ZOOLOGIQUE BELGE AUX ILES GALAPAGOS ET EN ECUADOR. ed by T. E. Mittler, C. N. Smith and H. V. Resh     

REVIEWS

Book reviewed in this article:
Salt Marshes and Salt Deserts of the World . By V. J. C hapman . Edited by N. P olunin .
The Measurement of Grassland Productivity . Proceedings of the University of Nottingham Sixth Easter School in Agricultural Science. Edited by Professor J. D. I vins
Intermediary Metabolism in Plants . By D. D. D avies .
Nomenclature of Plants . A Text for the Application by the Case Method of the International Code of Botanical Nomenclature. By H arold S t . J ohn .
Biological Clocks . Cold Spring Harbor Symposia on Quantitative Biology.
A Taxonomic Revision of the Humiriaceae . By J. C uatrecasas .
Models and Analogues in Biology . Fourteenth Symposium of the Society for Experimental Biology.
Encyclopaedia of Plant Physiology. Vol. XI, Parts 1 and 2. Edited by W. R uhland .
Protoplasmatologia. Handbuch der Protoplasmaforschung , Vol. II, C8d. Permeabilitäts-theorien . By V. W artiovaara and R. C ollander .
Handbuch der Pflanzenphysiologie . Edited by W. R uhland . Volume V: The Assimilation of Carbon Dioxide.
Die Pflanzenwelt der Tschechoslovakei . Ergebnisse der 12. internationalen pflanzengeo-graphischen Excursion (IPE) durch die Tschechoslovakei, 1958. Edited by W erner L üdi .
Macromolecular Complexes . Edited by M. V. E dds , J r .
Trace Elements in Plants . By W. S tiles .     

REVIEWS

Diseases of Fruits and Hops. By H. W ormald .
Nytteplanter. By K. G ram , H. J. J ensen and A. M entz .
Poisonous Plants of the United States. By W. C. M uenscher .
Introduction to the Botany of Field Crops. By Prof. J. M. H ector .
Commercial Fruit and Vegetable Products: a Textbook for Student, Investigator and Manager. By W. V. C ruess .
The Rape of the Earth: A World Survey of Soil Erosion. By G. V. J acks and R. O. W hyte .
Science in Africa: A Review of Scientific Research relating to Tropical and Southern Africa. By E. B. W orthington .
Health and Nutrition in India. By N. G angulee .
An Introduction to Modern Genetics. By C. H. W addington .
Earth's Green Mantle. By S. M angham .     

REVIEWS

Book reviewed in this article:
Plant Cells . By F. A. L. C lowes and B. E. J uniper .
The History of British Vegetation . By W inifred P ennington . Modern Biology: General Editor J. E. W ebb .
Flora Europaea , Vol. II. Rosaceae to Umbelliferae . Ed. by T. G. T utin , V. H. H eywood , N. A. B urges , D. M. M oore , D. H. V alentine , S. M. W alters and D. A. W ebb .
Desert Wild Flowers . By E dmund J aeger .
British Mosses and Liverworts . By E. V. W atson .
Anatomy of the Monocotyledons , Vol. III. Commelinales—Zingiberales . By P. B. T omlinson . General Editor C. R. M etcalfe .
Biological Science—The Web of Life . Supervising Editor, D. M organ .
Plant Chimeras . By W. N eilson -J ones .
The Potatoes of Argentine, Brazil, Paraguay and Uruguay, a Biosystematic Study . By J. G. H awkes and J. P. H jerting .
Towards an Understanding of the Mechanism of Heredity . By H. L. K. W hitehouse .
Viruses in Plant Hosts; Form, Distribution and Pathologic Effects . By K atherine E sau .
An Introduction to Industrial Mycology . By G eorge S mith .     

Book reviews

Book reviewed in this article:
BLOODSUCKING MIDGES OF THE GENERA CULICOIDES AND FORCIPOMYIA (CERATOPOGONIDAE). By V. M
BLOODSUCKING MIDGES OF SIBERIA AND THE FAR EAST. By A. G. Mirzaeva     

REVIEWS

Book reviewed in this article:
Botanical Histochemistry: Principles and Practice . By W illiam A. J ensen .
The Biology of Cilia and Flagella . By M. A. S leigh .
Dünnschicht-Chromatographie . Edited by E gon S tahl .
Plant Embryology. A Symposium .
The Morphology of Pteridophytes . By K. R. S porne .
Introductory Mycology . By C. J. A lexopoulos .
Residue Reviews , Vol. 1. Edited by F. A. G unther .
Energy Exchange in the Biosphere . By D avid M. G ates .
Pathology of Trees and Shrubs with Special Reference to Britain . By T. R. P eace .
Modern Methods of Plant Analysis . Vol. V. Edited by H. F. L inskens and M. V. T racey .
The Growth of Plants . By G. E. F ogg .
An Introduction to Plant Physiology . By W. O. J ames .
Die Physiologische Uhr. Zeitmessung in Organismen mit ungefähr Tagesperiodischen Schwingungen . By E. B ünning .     

BOOK REVIEWS

Books Reviewed in this Article:
BIOLOGIE ET EXPLOITATION DES POISSONS DU LACIHEMA (BASSIN AKAGERA, RWANDA). By P. D. Plisnier, J-C. Micha and V. Frank.
A HISTORY OF MARINE FISH CULTURE IN EUROPE AND NORTH AMERICA. By R. Kirk.
SALMON AND TROUT FARMING. Edited by Lindsay Laird and Ted Needham.
BOOKS RECEIVED
CHESAPEAKE BAY ENVIRONMENTAL DATA DIRECTORY. Compiled by Dan Jacobs et al.
GENETISCHE GRUNDLAGEN DER FISCHZÜCHTUNG. By V. S. Kirpičnikov.
ADVANCES IN MARINE BIOLOGY, Volume 24. Edited by J. H. S. Blaxter and A. J. Southward.
AN INTRODUCTION TO MARINE ECOLOGY (2nd edition). By R. S. K. Barnes and R. N. Hughes.     

Expression and purification of recombinant human annexin V in Escherichia coli

Human annexin V cDNA was cloned into plasmid pET19b and fused to a ten consecutive histidine tag at N-terminal. When expressed in E. coli BL21(DE3) LysS, the recombinant His10-annexin V accumulated in soluble form in the cytoplasm. By two-step chromatography, i.e., metal chelate affinity chromatography and anion exchange chromatography, recombinant His10-annexin V was purified to homogeneity on silver-stained SDS-PAGE gel. Recombinant annexin V, 7.4 mg, was obtained from a 1 litre flask culture.     

REVIEWS

Grazing in Terrestrial and Marine Environments (British Ecological Society Symposium No. 4). Edited by D. J. C risp
Fundamentals of Botany Series . Edited by W. A. J ensen and L. G. K avaljian
Multivariate Statistical Analysis for Biologists . By H ilary S eal
Differenciation des Cellules Sexuelles et Eécondation chez les Cryptogames (Protoplasmatologia , Vol. VII/3b). By B. V azart
Allgemeine Botanik . By W. N ultsch
Piante Medicinali, Chimica, Farmacologia e Terapia . Vol. II. By R. B enigni , C. C apra and P. E. C attorini
Thermophilic Fungi . By D onald G. C ooney and R alph E merson
The Genera of Flowering Flants (Angiospermae). Based Principally on the Genera Plantarum of G. Bentham and J. D. Hooker. Dicotyledones . vol. 1. By J. H utchinson
Algal Cultures and Phytoplankton Ecology . By G. E. F ogg
Flora Europaea, Vol . 1. Eycopodiaceae to Plantanaceae . Edited by T. G. T utin , V. H. H eywood , N. A. B urges , D. H. V alentine , S. M. W alters and D. A. W ebb
The Biology of the Algae . By F. E. R ound
Flora of the British Isles Illustrations . Part 4. Monocotyledons . Drawings by S ybil J. R oles
Plants and the Ecosystem . By W. D. B illings . (Fundamentals of Botany Series, edited by W. A. J ensen and L. G. K avaljian
Essays on Crop Evolution . Edited by S ir J oseph H utchinson     

Book Notices

Bumblebees. By D. V. A lford
Stick and Leaf Insects. By J. T. C lark
The World of the Honeybee. By C olin G. B utler . Third edition
The Biology of Tribolium with Special Emphasis on Genetic Aspects. By A. S okoloff
The Use of Genetics in Insect Control. Edited by R. P al and M. J. W hitten