共查询到20条相似文献,搜索用时 15 毫秒
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Mapping of the SV40 specific sequences transcribed in vitro from chromatin of SV40 transformed cells. 总被引:4,自引:0,他引:4
S M Astrin 《Biochemistry》1975,14(12):2700-2704
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Activation of the major drosophila heat-shock genes in vitro 总被引:3,自引:0,他引:3
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Transcription of chromatin by human RNA polymerase 总被引:3,自引:0,他引:3
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Transcription of fractionated mammalian chromatin by mammalian ribonucleic acid polymerase. Demonstration of temperature-dependent rifampicin-resistant initiation sites in euchromatin deoxyribonucleic acid 总被引:4,自引:2,他引:2 下载免费PDF全文
C. James Chesterton Barbara E. H. Coupar Peter H. W. Butterworth 《The Biochemical journal》1974,143(1):73-81
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The effects of 4-thiouridine and its metabolites on RNA synthesisin radish (Raphanus sativus) cotyledons were investigated. The4-thiouridine nucleotides, 4-thioUTP and 4-thioUDP, were foundto inhibit DNA-dependent RNA polymerase activities of isolatednuclei and of chloroplasts from radish cotyledons. These inhibitorsappeared to compete with UTP for its binding to RNA polymerase.Neither 4-thiouridine nor 4-thioUMP inhibited RNA polymeraseactivities. Reduced RNA accumulation accompanied by the inhibition of protochlorophyllideaccumulation was observed in cotyledons of dark-grown radishseedlings germinated and grown with 4-thiouridine. On the otherhand, 4-thiouridine had no effect on chloroplast developmentin the control seedlings germinated and grown without 4-thiouridine.These results suggest that the inhibition of chloroplast developmentby 4-thiouridine may in part be due to the inhibition of RNAsynthesis by 4-thiouridine nucleotide during germination, resultingin inhibition of etioplast development. (Received December 9, 1985; Accepted June 26, 1986) 相似文献
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