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We have developed a nonradioactive oligonucleotide multilocus DNA fingerprinting method for Cynoglossum officinale . Of the 19 probes tested, six probes yielded banding patterns for all restriction enzymes used. All but one of the informative probes are repeats with a four-base motif. Approximately 60% of the loci appeared to be polymorphic. The sensitivity of the nonradioactive method was equal to that of the radioactive method. In addition, a new simple calculation method is presented to estimate selfing rates and approximate 95% confidence limits from the DNA fingerprint profiles avoiding 'between-gel' comparisons. The selfing rates differed significantly (as determined from 95% confidence intervals) between naturally pollinated individuals of C. officinale within the experimental population. The estimates ranged from 0 to 70% selfing. 相似文献
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As with other plants having a relatively simple morphology, solitary palms are useful biological models for studying the life histories of long-lived plants. In the first study to investigate the life history of Borassus aethiopum, a widespread dioecious palm growing in African savannas, we found that: (1) the number of leaves increased up to reproductive maturity and then decreased during the reproductive period, while height increased throughout life; (2) female fecundity, measured as the number of seedlings within a few meters under the female canopies, decreased markedly in old individuals with few leaves; and (3) height distribution was strongly bimodal. This bimodality was due to variations in the stem elongation rate during the life of the palm and the accumulation of adults with low mortality rates in the taller height classes. The observed pattern of fecundity and number of leaves in relation to height clearly suggests a senescence period that began just after sexual maturity and appeared to be protracted. Comparisons between the life history of B. aethiopum and the life histories of some forest palms showed that environmental conditions cannot in themselves explain the various palm life histories. 相似文献
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Andrea Matern Claudia Drees Hartmut Meyer Thorsten Assmann 《Journal of Insect Conservation》2008,12(6):591-601
The declining European ground beetle Carabus variolosus, an indicator of undisturbed woodland brooks and swamps, is listed in the EU Species and Habitats Directive. Little is known
about this flightless and endangered beetle, apart from its distribution range and habitat stenotopy. We present the results
of a 2-year mark-release-recapture study on two neighbouring populations in Germany, which investigated basic parameters of
population ecology that may be influential in the persistence of the beetle. Extremely small population sizes were the key
findings. Since population densities corresponded to those of typical Carabus species, the size of the habitats was most probably the limiting factor. The risk of heavy losses during offspring development
and hibernation may be alleviated by the considerable proportion of C. variolosus reproducing in more than 1 year. The fact that we did not recapture any marked specimens from the neighbouring population
suggests a very limited dispersal power. The results imply a high vulnerability of the species, provide basic methodological
information for an implementation of the EC Directive and for further research, and highlight the necessity to enlarge populated
habitats. 相似文献
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Exotic grasses and grass-fueled fires have altered plant species composition in the seasonal submontane woodlands of Hawaii Volcanoes National Park. These changes have altered both structural and functional aspects of the plant community, which could, in turn, have consequences for litter decomposition and nitrogen (N) dynamics. In grass-invaded unburned woodland, grass removal plots within the woodland, and woodland converted to grassland by fire, we compared whole-system fluxes and the contributions of individual species to annual aboveground fine litterfall and litterfall N, and litter mass and net N loss. We assessed the direct contribution of grass biomass to decomposition and N dynamics, and we determined how grasses affected decomposition processes indirectly via effects on native species and alteration of the litter layer microenvironment. Grasses contributed 35% of the total annual aboveground fine litterfall in the invaded woodland. However, total litterfall mass and N were not different between the invaded woodland and the grass removal treatment because of compensation by the native tree Metrosideros polymorpha, which increased litter production by 37% ± 5% when grasses were removed. The 0.3 g N m–2/y–1 contained in this production increase was equal to the N contained in grass litter. Litter production and litterfall N was lowest in the grassland due to the loss of native litter inputs. Decomposition of litterfall on an area basis was highest in the grass-invaded woodland. We attributed this effect to increased inherent decomposability of native litter in the presence of grasses because (a) the microenvironment of the three vegetation treatments had little effect on decomposition of common litter types and (b) M. polymorpha litter produced in the invaded woodland decomposed faster than that produced in the grass removal plots due to higher lignin concentrations in the latter than in the former. Area-weighted decomposition was lowest in the grassland due to the absence of native litter inputs. Across all treatments, most litter types immobilized N throughout the incubation, and litter net N loss on an area basis was not different among treatments. Our results support the idea that the effects of a plant species or growth form on decomposition cannot be determined in isolation from the rest of the community or from the direct effects of litter quality and quantity alone. In this dry woodland, exotic grasses significantly altered decomposition processes through indirect effects on the quantity and quality of litter produced by native species. 相似文献
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用巴拿马50 hm2森林动态监测样地内直径≥1 cm的树种资料,分析了该样地树种多度(个体数)和丰富度(物种数)及其方差和变异系数在6个取样尺度(5 m×5 m,10 m×10 m,20 m×20 m,25 m×25 m,50 m×50 m,100 m×100 m)的变化规律.结果显示:(1)由于多度的可加性,不同取样尺度在样地内树种多度的变化表现出一致性;随取样尺度的增加,多度方差呈线性增加,而变异系数呈线性减小.(2)丰富度随取样尺度的变化较为复杂,随取样尺度的增加,丰富度方差呈非线性变化,在取样尺度为25 m×25 m时方差最大;变异系数随取样尺度的增加而呈线性减小.研究表明,大尺度的多度值可以由小尺度的多度值通过外推法估计,而丰富度却不能,在生物多样性的保护和管理中不能简单地从一个取样尺度的生物丰富度推测另一个取样尺度丰富度. 相似文献
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Claus Orendt 《Journal of Insect Conservation》2000,4(2):79-91
The distribution of chironomid species from four springs and twelve spring brooks in a region heavily affected by industrial activity in eastern Germany was studied. The aims were (1) to document the chironomid taxa communities at the species level, as far as possible, and the most important environmental factors of representative springs and spring brooks of the region, (2) to analyse the distribution of the ecological groups within these small ecosystems and (3) to emphasize the importance of the protection of spring areas and spring brooks in this woodland area shown by the chironomid fauna. There was high degree of abiotic variation among the water bodies, with pH ranging from 2.79 to 6.89. While diversity did not correlate with pH nor with conductivity, significant differences existed between the spring mouth and the reaches some metres downstream with respect to several abiotic parameters and the distribution of the chironomids: oxygen concentrations and taxonomic diversities were higher in spring brooks (2.18–8.98mgO2/l; 5–28 taxa) than in the spring areas (0.13–2.95mgO2/l; 0–7 taxa). A total of 74 chironomid taxa were recorded. The chironomid communities are characterized by the presence of rheophilic, cold-stenothermic, crenophilic and sometimes tyrphophilic elements in each site. The data show that each of the spring–spring brook systems surveyed is unique both in biotic and abiotic respects. As the spring system is sensitive to changes in landscape functions (e.g. groundwater levels), the fauna can serve as an indicator of landscape health. Therefore, these small ecosystems should be conserved as a reference. 相似文献
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Soil acidity and calcium (Ca) availability in the surface soil differ substantially beneath sugar maple (Acer saccharum) and eastern hemlock (Tsuga canadensis) trees in a mixed forest in northwestern Connecticut. We determined the effect of pumping of Ca from deep soil (rooting zone below 20-cm mineral soil) to explain the higher available Ca content in the surface soil beneath sugar maple. We measured the atmospheric input of Ca with bulk deposition collectors and estimated Ca weathering and Ca mineralization in the surface soil (rooting zone above 20-cm mineral soil) from strontium isotope measurements and observed changes in exchangeable Ca in soils during field incubation. Calcium leaching at 20 cm was calculated by combining modeled hydrology with measured Ca soil solution concentrations at 20-cm depth. We measured root length distribution with depth beneath both tree species. Calcium leaching from the surface soil was much higher beneath sugar maple than hemlock and was positively related with the amount of Ca available in the surface soil. Calcium leaching from the surface soil beneath sugar maple was higher than the combined Ca input from atmospheric deposition and soil weathering. Without Ca uptake in the deep soil, surface soils are being depleted in Ca, especially beneath sugar maple. More organically bound Ca was mineralized beneath sugar maple than beneath hemlock. A relatively small part of this Ca release was leached below the surface soil, suggesting that, beneath both tree species, most of the Ca cycling is occurring in the surface soil. Sugar maple had more fine roots in the deep soil than hemlock and a greater potential to absorb Ca in the deep soil. With a simple model, we showed that a relatively small amount of Ca uptake in the deep soil beneath sugar maple is able to sustain high amounts of available Ca in the surface soil. Received 20 June 2001; accepted 6 December 2001. 相似文献
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根据 2 2个等位酶位点遗传变异 ,探讨了韩国境内委陵菜 (PotentillafragarioidesL .var.sprengeliana)的遗传多样性和种群结构。酶位点的多态位点百分比为 5 9 1%。种和种群水平上的遗传多样性比较高 ,分别为Hes=0 .2 10 ,Hep=0 .199;而种群的分化水平则相对较低 (GST=0 .0 74)。 19个种群中随机交配的偏差为FIS=0 .331。每代迁移数的间接估计 (Nm=3.15 )表明该种在韩国的种群间基因流较高。另外 ,固定指数分析显示在一些种群和位点有轻微的杂合子缺乏。种群间平均遗传一致度为 0 985。这些韩国委陵菜种群存在于较为均一的生境 ,这很有可能是造成其种群遗传一致性较高的原因。 相似文献
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Paul D. Pratt Min B. Rayamajhi Thai K. Van Ted D. Center 《Biocontrol Science and Technology》2004,14(1):51-62
The Australian snout beetle, Oxyops vitiosa, was introduced to south Florida, USA, as a biological control agent of the invasive tree Melaleuca quinquenervia during the spring of 1997. As part of ongoing post-release evaluations of this weevil, we sought to quantify the population density that can be maintained by O. vitiosa larvae when fully exploiting the available melaleuca foliage. Seasonal population densities were modeled as a function of tree size distribution and density, plant phenology, quantity of acceptable foliage (suitable for larval development) and larval consumption values. Larval densities varied dramatically due to fluctuating resource availability and ranged from 830 946 larvae/ha in July–August to >4.5 million larvae/ha in January–February. The expected population density for a generalized (mixed sized) tree distribution was estimated to be 13.9 million larvae/ha. Larval densities increased to 18.8 million larvae/ha per year when large trees (>20 cm diameter at breast height) represented the dominant size class, whereas habitats dominated by medium (10–19 cm) and small (1.3–9 cm) trees were predicted to have larval densities of 11.3 and 6.3 million/year, respectively. Validation data obtained from smaller, more accessible plants suggested that the model overestimated realized yearly larval densities by 9% or 15 416 larvae/ha per year. 相似文献
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樟翠尺蛾种群动态与植物群落结构及气候因子的关系 总被引:2,自引:1,他引:2
在昆虫种群的研究中,Gieir和Clark等曾经提出生命系统的基本概念[1,2],认为生命系统由一个对象种群和作用于这个种群的环境所组成。控制昆虫种群增长的有密度制约因子(如食源)和非密度制约因子(如气候环境)。昆虫和植物之间的关系,历来是生态学研究的重要领域。通过长期的定点监测,研究某种昆虫种群动态与各种生态因子之间的关系,不仅可揭示动物和植物及环境之间互相影响的关系,对保护生物学理论的某些方面将有所发展,而且可为林业生产和环保工作提供如何应用生态学原理控制虫害,保护生物多样性和维护生态平衡的理论依据。樟翠尺… 相似文献
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Martin Konvicka Jan Novak Jiri Benes Zdenek Fric Jonathan Bradley Petr Keil Jan Hrcek Karel Chobot Pavel Marhoul 《Journal of Insect Conservation》2008,12(5):549-560
The distribution of Lopinga achine (Lepidoptera Nymphalidae, Satyrinae) in the Czech Republic has declined from thirty grid squares before 1950 to just one extant population, restricted to a single area of deciduous woodland. A review of historical sites shows that this species used to occur in various types of deciduous woodland with a relatively sparse canopy maintained by coppicing and/or grazing. The extant population inhabits mature woodland with a mean canopy cover of 60% (quartiles 50% and 65%), sparse shrubs and a species-rich herb layer containing plant species requiring dry, warm and nutrient-poor conditions. The larval host plants are the fine-leafed sedges, Carex fritschii and C. michelii. In 2006, the total population contained about 10,000 adults but this may be an over-estimate, biased by male behaviour. Measurements of adult mobility, well approximated by an inverse-power function, suggested that all existing colonies are interconnected by dispersal. Continuing existence of the population depends on two conditions; nutrient-poor conditions for a diverse ground flora and a sparse tree canopy. While canopy closure is gradually increasing, the herb layer is threatened by soil enrichment due to the demise of traditional grazing, litter raking and grass mowing in woodlands. Any future management to favour Lopinga achine should include both measures to maintain a sparse canopy and measures to export biomass, such as raking or mowing of ground flora or, preferably, re-establishment of grazing. An erratum to this article can be found at 相似文献
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Mature tropical forests at agricultural frontiers are of global conservation concern as the leading edge of global deforestation.
In the Ituri Forest of DRC, as in other tropical forest areas, road creation associated with selective logging results in
spontaneous human colonization, leading to the clearing of mature forest for agricultural purposes. Following 1-3 years of
cultivation, farmlands are left fallow for periods that may exceed 20 years, resulting in extensive secondary forest areas
impacted by both selective logging and swidden agriculture. In this study, we assessed forest structure, tree species composition
and diversity and the regeneration of timber trees in secondary forest stands (5-10 and ~40 years old), selectively logged
forest stands, and undisturbed forests at two sites in the Ituri region. Stem density was lower in old secondary forests (~40 years
old) than in either young secondary or mature forests. Overall tree diversity did not significantly differ between forest
types, but the diversity of trees ≥10 cm dbh was substantially lower in young secondary forest stands than in old secondary
or mature forests. The species composition of secondary forests differed from that of mature forests, with the dominant Caesalpinoid
legume species of mature forests poorly represented in secondary forests. However, in spite of prior logging, the regeneration
of high value timber trees such as African mahoganies (Khaya anthotheca and Entandrophragma spp.) was at least 10 times greater in young secondary forests than in mature forests. We argue that, if properly managed
and protected, secondary forests, even those impacted by both selective logging and small-scale shifting agriculture, may
have high potential conservation and economic value. 相似文献
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Mark S. Johnson Eduardo Guimar?es Couto Osvaldo B. Pinto Jr Juliana Milesi Ricardo S. Santos Amorim Indira A. M. Messias Marcelo Sacardi Biudes 《PloS one》2013,8(6)
The Pantanal is a biodiversity hotspot comprised of a mosaic of landforms that differ in vegetative assemblages and flooding dynamics. Tree islands provide refuge for terrestrial fauna during the flooding period and are particularly important to the regional ecosystem structure. Little soil CO2 research has been conducted in this region. We evaluated soil CO2 dynamics in relation to primary controlling environmental parameters (soil temperature and soil water). Soil respiration was computed using the gradient method using in situ infrared gas analyzers to directly measure CO2 concentration within the soil profile. Due to the cost of the sensors and associated equipment, this study was unreplicated. Rather, we focus on the temporal relationships between soil CO2 efflux and related environmental parameters. Soil CO2 efflux during the study averaged 3.53 µmol CO2 m−2 s−1, and was equivalent to an annual soil respiration of 1220 g C m−2 y−1. This efflux value, integrated over a year, is comparable to soil C stocks for 0–20 cm. Soil water potential was the measured parameter most strongly associated with soil CO2 concentrations, with high CO2 values observed only once soil water potential at the 10 cm depth approached zero. This relationship was exhibited across a spectrum of timescales and was found to be significant at a daily timescale across all seasons using conditional nonparametric spectral Granger causality analysis. Hydrology plays a significant role in controlling CO2 efflux from the tree island soil, with soil CO2 dynamics differing by wetting mechanism. During the wet-up period, direct precipitation infiltrates soil from above and results in pulses of CO2 efflux from soil. The annual flood arrives later, and saturates soil from below. While CO2 concentrations in soil grew very high under both wetting mechanisms, the change in soil CO2 efflux was only significant when soils were wet from above. 相似文献
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N. Chaffey 《Protoplasma》1985,127(1-2):128-132
Summary Using phosphotungstic acid and periodic acid/thiocarbohydrazide/silver proteinate (Thiéry test) aspects of polysaccharide ultracytochemistry were studied in the membranous ligule ofLolium temulentum L. Staining results are presented for all three tissues-abaxial and adaxial epidermes and mesophyll-but discussed only for the epidermes. PTA- and PATAg-staining of the adaxial epidermis suggested synthesis of a conjugated polysaccharide material in this tissue, its accumulation in the periplasmic space and its subsequent secretion to the outside of the ligule via gaps in the cuticle. The ligule of this grass is considered to be a secretory organ. 相似文献
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采用RAPD和ISSR标记研究古尔班通古特沙漠东南缘梭梭种群的遗传结构 总被引:5,自引:0,他引:5
梭梭(Haloxylon ammodendron (CA Mey.) Bunge)是一种沙漠旱生优势树种,具有重要的生态和经济价值,然而,我们对梭梭种群的遗传多样性和遗传结构所知甚少。本文采用RAPD和ISSR标记对来自古尔班通古特沙漠东南缘的4个天然梭梭种群的遗传多样性和遗传结构进行了检测。5个RAPD引物和8个ISSR引物分别扩增出61和195条带,多态性位点比率分别为83.6%和89.7%,Shannon 信息指数分别为0.333和0.367,RAPD和ISSR分析均表明梭梭种群的遗传多样性水平较高。利用分子方差分析(AMOVA)研究梭梭种群的遗传结构,结果表明,大部分遗传变异存在于种群内,通过RAPD分析发现138.2%的遗传变异发生在种群内;通过ISSR分析发现89.4%的遗传变异发生在种群内;而种群间的遗传分化很小。通过RAPD标记没有检测到种群间的遗传分化, ISSR分析表明10.6%的遗传变异发生在种群内。我们推测梭梭种群较高的遗传多样性水平可能源于对异质、高胁迫环境的长期适应,但还需要进一步的研究加以证实。种群间遗传分异低的主要原因是种群间存在强大的基因流。 相似文献