A convenient method for the isolation of 3'-sialyllactose from normal human urine

A simple method for the preparation of 3'-sialyllactose from normal human urine is described. The method for the collection of sialooligosaccharides is based on adsorption on charcoal followed by elution with a mixture of ethanol, pyridine, and water. The sialooligosaccharide mixture is then fractionated by means of gel filtration on Sephadex G-25 and the low molecular weight fraction is further fractionated on Dowex 1 (eluted with pyridinium acetate) to give five fractions. The last fraction contains almost pure 3'-sialyllactose.     

A rapid and convenient method for purification and isolation of chlorophyll-A from Porphyra yezoensis.

A rapid and convenient method for purification and isolation of chlorophyll-a from Porphyra yezoensis without any chromatographic procedures has been developed. Thin-layer chromatographic and high-performance liquid chromatographic tests revealed that chlorophyll-a preparations in this study did not contain any other photosynthetic pigments and their degradation products.     

A convenient method for isolation of murine erythrocyte catalase by gel filtration and DEAE cellulose chromatography

A method for isolation of catalase from small volumes of murine red blood cells is described. The purified enzyme had a specific activity of 25,000 IU/mg protein and was judged to be homogeneous by polyacrylamide and sodium dodecyl sulphate electrophoresis. The specific activity of the isolated murine erythrocyte catalase is approximately 14–25% that of human erythrocyte catalase isolated by the method described.     

A convenient method for detecting sulphate-reducing bacteria

Sulphate-reducing bacterial cells, after addition of sodium hydroxide, showed a pink colour under a visible light microscope. This phenomenon is suggested as a test for sulphate-reducing bacteria.     

A convenient method for preparative peptide separation

A chromatographic system has been developed for the separation of peptides on a preparative scale. The principle of the method is to use the peptide bond absorbance in the 200–250 nm wavelength range as the basis for the peptide detection, and therefore to use only inorganic buffers that are transparent at these low wavelengths. Chromatography on phosphocellulose and on triethylaminoethyl-(TEAE-)cellulose leading to complete separation of the tryptic peptides of oxidized pancreatic ribonuclease is reported as an illustration of the method. The new system, which can be used both rapidly and conveniently and with simple equipment, appears to have broad applicability to peptide separation.     

A convenient purification method for pyridylamino monosaccharides

Pyridylamino derivatization is suitable for the microanalysis of sugars, but there is a problem in that by-products of the labeling reaction and fluorescent substances from samples occasionally interfere with the detection of pyridylamino sugars. Especially, interference by them is serious in monosaccharide analysis. I have developed a convenient purification method for pyridylamino monosaccharides by the use of a spin column with boronate-conjugated resin.     

A convenient method for the ATPase assay.

A new method for the determination of inorganic phosphorus released in ATPase assay has been evaluated. The method is based on the reduction of a phosphomolybdate complex by Elon in a copper acetate buffer. In contrast to current methods, there is no interference by ATP with color development. There is also less or no interference by other compounds usually present in ATPase assay media. The method is simple, sensitive, and reproducible.     

一种提纯粗茶皂素的简易方法

对粗茶皂素的提取纯化工艺进行了研究。以质量分数为70％的粗茶皂素为原料,经2％NaOH溶解、盐酸酸析、95％乙醇溶解、丙酮沉析工艺得到精制茶皂素。检测结果表明,茶皂素质量分数大于95％,提取率大于80％,这种简便易行的工艺得到的茶皂素可作为开发新型植物灭螺剂的原料。     

A convenient method of isoflavonone synthesis

Reaction of 2-hydroxydeoxybenzoins with bis(dimethylamino)methane in ethanol results in isoflavonones.     

A convenient method of isoflavonone synthesis

Reaction of 2-hydroxydeoxybenzoins with bis(dimethylamino)methane in ethanol results in isoflavonones.     

A simple and convenient method for acyclonucleoside synthesis.

Introduction of acyclic chain for synthesis of acyclonucleoside derivatives was achieved in a simple and convenient way. Silylated pyrimidine or purine bases were treated with 1,3-dioxolane, trimethyl chlorosilane and metal iodide, such as KI and NaI, all together at room temperature. By this method, 2-thiopyrimidine derivatives were also obtained in good yield, using 2 molecular equivalents of 1,3-dioxolane.     

A convenient method for potentiometric titration of chloride ions

A convenient reference cell and method of potentiometric determination of chloride ions with silver nitrate is described. The method is accurate to about +/- 0.02 ml. 10(-4)M silver nitrate.