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1.
Random amplified polymorphic DNAs (RAPDs) were used to construct linkage maps of the parent of a longleaf pine ( Pinus palustris Mill.) slash pine ( Pinus elliottii Englm.) F 1 family. A total of 247 segregating loci [233 (1∶1), 14 (3∶1)] and 87 polymorphic (between parents), but non-segregating, loci were identified. The 233 loci segregating 1∶1 (testcross configuration) were used to construct parent-specific linkage maps, 132 for the longleaf-pine parent and 101 for the slash-pine parent. The resulting linkage maps consisted of 122 marker loci in 18 groups (three or more loci) and three pairs (1367.5 cM) for longleaf pine, and 91 marker loci in 13 groups and six pairs for slash pine (952.9 cM). Genome size estimates based on two-point linkage data ranged from 2348 to 2392 cM for longleaf pine, and from 2292 to 2372 cM for slash pine. Linkage of 3∶1 loci to testcross loci in each of the parental maps was used to infer further linkages within maps, as well as potentially homologous counterparts between maps. Three of the longleaf-pine linkage groups appear to be potentially homologous counterparts to four different slash-pine linkage groups. The number of heterozygous loci (previously testcross in parents) per F 1 individual, ranged from 96 to 130. With the 87 polymorphic, but non-segregating, loci that should also be heterozygous in the F 1 progeny, a maximum of 183–217 heterozygous loci could be available for mapping early height growth (EHG) loci and for applying genomic selection in backcross populations. 相似文献
5.
A climate model with time varying parameters was fit to longleaf pine ( Pinus palustris) tree rings collected from the proposed Harrison Research Natural Area of the De Soto National Forest in southern Mississippi. The purpose of the analysis was to determine if any unexpected disturbances had influenced the growth of the trees. Current September temperature, August rainfall and February Palmer Drought Severity Index (PDSI) were found jointly to be the best variables in the model to predict growth. August rain had a constant significant effect on growth and February PDSI was not significant except between the years 1968–1983. It was concluded that the Harrison area has been in equilibrium for the past 50 years since no apparent outside influences have caused the trees to become less sensitive to climate. Thus, the forest is a good candidate for a Research Natural Area.Abbreviations (RNA)
Research Natural Area 相似文献
6.
The longleaf pine (LLP) savanna ecosystem once covered?~?92 million acres of the Southeast USA, but due to anthropogenic activities such as logging and fire suppression, only 3% of its once widespread historic range remains. While many restoration efforts are underway to conserve this biodiverse ecosystem, restoration must be done in the context of climate change. In the last few decades, heatwaves have increased in frequency and intensity across the Southeastern USA with further increases predicted. To expand our understanding of LLP savanna restoration in light of these changes, we ran a series of three simulated heatwave greenhouse experiments through a Course-based Undergraduate Research Experience (CURE) incorporating?~?150 undergraduate researchers per experiment. We measured plant growth metrics for four understory grasses commonly used in LLP savanna restoration efforts. We found that while most grass plug individuals survived heatwave conditions, aboveground production was reduced due to heatwaves. This productivity decrease could result in less biomass available for the essential vegetation fire feedback loop, where fire increases grass biomass, and in turn, more grass provides more fuel for fire. These results imply that land managers can proactively compensate for biomass loss due to heatwaves by planting more grass plugs during initial restoration. 相似文献
7.
Imperata cylindrica is an invasive C 4 grass, native to Asia and increasing in frequency throughout the tropics, subtropics, and southeastern USA. Such increases are associated with reduced biodiversity, altered fire regimes, and a more intense competitive environment for commercially important species. We measured rates of clonal spread by I. cylindrica from a roadside edge into the interior of two longleaf pine savannas. In addition, we measured the effects of fertilization with nitrogen and phosphorus on clonal invasion of one of these sites. Clonal invasion occurred at both sites and at similar rates. Older portions of an I. cylindrica sward contained fewer species of native pine-savanna plants. Clonal growth rates and aboveground mass of I. cylindrica were reduced by the addition of phosphorus relative to controls by the second growing season at one site. As a group, native species were not affected much by P-addition, although the height of legumes was increased by P addition, and the percent cover of legumes relative to native non-legumes decreased with increasing expected P limitation (i.e., going from P-fertilized to controls to N-fertilized treatments). Clonal invasion was negatively correlated with the relative abundance of legumes in control plots but not in P-fertilized plots. Species richness and percent cover of native plants (both legumes and non-legumes) were dramatically lower in N-fertilized plots than in controls or P-fertilized plots. Species richness of native plants was negatively correlated with final aboveground mass of I. cylindrica in control and P-fertilized plots, but not in N-fertilized plots. The results suggest that I. cylindrica is a better competitor for phosphorus than are native pine-savanna plants, especially legumes, and that short-lived, high-level pulses of phosphorus addition reduce this competitive advantage without negatively affecting native plant diversity. Ratios of soil P to N or native legume to non-legume plant species may provide indicators of the resistance of pristine pine savannas to clonal invasion by I. cylindrica. 相似文献
9.
A 35-year-old longleaf pine stand exhibited trees in various stages of decline. A study was conducted to determine root-infecting
fungi and other abnormalities associated with varying degrees of crown symptoms. A four-class crown symptom rating system
was devised according to ascending symptom severity. Leptographium procerum and L. terebrantis were significantly associated
with increasing crown symptom severity. Heterobasidion annosum was also isolated in higher frequency as crown symptoms increased.
Also, evidence of insects on roots increased as did amount of resinosis observed. Edaphic and silvicultural factors may interact
with these pathogens and insects to pose a pathological limitation on longer-term management objectives. Further research
is needed to determine relationships among various edaphic, silvicultural, and biological factors associated with the decline
syndrome on this site.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
12.
The natural patterns of myrmecochory are disrupted by the dominance of red imported fire ants ( Solenopsis invicta) in the southeastern United States. This leads to questions about the role of fire ants as seed dispersers. We examined the fate of ant-dispersed seed in the longleaf pine ecosystem. First, we determined removal rates for a suite of common ground cover species. Then, we verified the final location of removed seeds by using a wax cast to examine nest contents, and locating dyed seeds deposited in trash piles on the ground surface. Finally, we determined if the germination rate of seeds deposited by fire ants was affected by ant dispersion. Fire ants were most attracted to elaiosome-bearing seed and collected nonelaiosome-bearing seed at a much lower rate. No seeds were found in the contents of wax castings of fire ant nest chambers, suggesting that seed is not stored within the nest. Of the dyed seeds that we presented to fire ants, 30–40 % were recollected in surface trash piles in the mound vicinity within 1 week following removal. Undiscovered seeds were considered destroyed or buried in foraging tunnels. A small percentage of the deposited seeds were able to germinate, but there was no difference in the percent germination between seeds manipulated by fire ants and the control. Low germination was likely due to a high percentage of immature seeds used in the study. Our findings support a growing body of evidence that fire ants facilitate the movement of seeds in the longleaf pine ecosystem. 相似文献
13.
Midstory hardwoods are traditionally removed to restore longleaf pine on fire‐excluded savannas. However, recent evidence demonstrating midstory hardwood facilitation on longleaf pine seedling survival has brought this practice into question on xeric sites. Also, midstory hardwoods could facilitate longleaf pine seedling establishment, as hardwood litter may conceal seeds from seed predators or improve micro‐environmental conditions for seedling establishment. However, little is known about these potential mechanisms. In this study, we tracked longleaf pine seed depredation and germination in artificially seeded plots (11 seeds/m 2) in a factorial design fully crossing hardwood retention or removal with vertebrate seed predator access or exclusion in the Sandhills Ecoregion of North Carolina, U.S.A. Seed depredation averaged 78% across treatments and was greatest in unexcluded plots. Hardwood retention did not affect seed depredation. Longleaf pine averaged 3.6 germinants/4 m 2 across treatments, and was six times more abundant where vertebrates had been excluded. Hardwood removal had a strong positive effect on seedling germination, likely due to the removal of litter, but only when vertebrates were excluded. Our results indicated midstory hardwoods are not facilitating longleaf pine seedling establishment. Nevertheless, our results indicated that hardwood removal may not increase longleaf pine seedling establishment, as seed depredation diminished the effectiveness of hardwood removal under mast seed availability. Collectively, these results demonstrate the underlying complexity of the longleaf pine ecosystem, and suggest that planting may need to be part of the restoration strategy on sites where seed depredation limits longleaf pine natural regeneration. 相似文献
14.
Summary 1. A line selected for high scutellar chaeta number reached a mean of about 16 chaetae in females and 13.5 in males at the 69th generation of selection following an accelerated response to selection which commenced at generation 65 and added five chaetae. 2. The accelerated response can probably be explained in terms of two recessive high chaeta number genes 1.05 cM apart, and which are located between po and vg on chromosome II. The gene closest to vg was found to be scabrous, sca, which causes rough eyes when homozygous and has a pleiotropic effect on scutellar chaeta number. The gene was found in one of the strains used in setting up the selection lines. 3. The results are discussed in relation to other theories of control of the scutellar chaeta system. 相似文献
15.
Summary Effects of variation in fire season on flowering of forbs and shrubs were studied experimentally in two longleaf pine forest habitats in northern Florida, USA. Large, replicated plots were burned at different times of the year, and flowering on each plot was measured over the twelve months following fire. While fire season had little effect on the number of species flowering during the year following fire, fires during the growing season decreased average flowering duration per species and increased synchronization of peak flowering times within species relative to fires between growing seasons. Fires during the growing season also increased the dominance of fall flowering forbs and delayed peak fall flowering. Differences in flowering resulting from variation in fire season were related to seasonal changes in the morphology of clonal forbs, especially fall-flowering composites. Community level differences in flowering phenologies indicated that timing of fire relative to environmental cues that induced flowering was important in determining flowering synchrony among species within the ground cover of longleaf pine forests. Differences in fire season produced qualitatively similar effects on flowering phenologies in both habitats, indicating plant responses to variation in the timing of fires were not habitat specific. 相似文献
16.
Statistical properties and extensions of Hedrick and Muona's method for mapping viability alleles causing inbreeding depression are discussed in this paper. Their method uses the segregation ratios among selfed progeny of a marker-locus heterozygote to estimate the viability reduction, s, of an allele and its recombination fraction, c, with the marker. Explicit estimators are derived for c and s, including expressions for their variances. The degree of estimation bias is examined for cases when (1) the viability allele is partially recessive and (2) the marker locus is linked to two viability loci. If linkage or viability reduction is moderate, very large sample sizes are required to obtain reliable estimates of c and s, in part because these estimates show a statistical correlation close to unity. Power is further reduced because alleles causing viability reduction often occur at low frequency at specific loci in a population. To increase power, we present a statistical model for the joint analysis of several selfed progeny arrays selected at random from a population. Assuming a fixed total number of progeny, we determine the optimal number of progeny arrays versus number of progeny per array under this model. We also examine the increase of information provided by a second, flanking marker. Two flanking markers provide vastly superior estimation properties, reducing sample sizes by approximately 95% from those required by a single marker. 相似文献
17.
The rates and controls of ectomycorrhizal fungal production were assessed in a 22-year-old longleaf pine ( Pinus palustris Mill.) plantation using a complete factorial design that included two foliar scorching (control and 95% plus needle scorch)
and two nitrogen (N) fertilization (control and 5 g N m −2 year −1) treatments during an annual assessment. Ectomycorrhizal fungi production comprised of extramatrical mycelia, Hartig nets
and mantles on fine root tips, and sporocarps was estimated to be 49 g m −2 year −1 in the control treatment plots. Extramatrical mycelia accounted for approximately 95% of the total mycorrhizal production
estimate. Mycorrhizal production rates did not vary significantly among sample periods throughout the annual assessment ( p = 0.1366). In addition, reduction in foliar leaf area via experimental scorching treatments did not influence mycorrhizal
production ( p = 0.9374), suggesting that stored carbon (C) may decouple the linkage between current photosynthate production and ectomycorrhizal
fungi dynamics in this forest type. Nitrogen fertilization had a negative effect, whereas precipitation had a positive effect
on mycorrhizal fungi production ( p = 0.0292; r
2 = 0.42). These results support the widely speculated but poorly documented supposition that mycorrhizal fungi are a large
and dynamic component of C flow and nutrient cycling dynamics in forest ecosystems. 相似文献
18.
In agricultural landscapes, the longleaf pine initiative (LLPI) and the Bobwhite Quail Initiative (BQI) aim to restore longleaf pine forests and early successional habitats, respectively. The early stage of longleaf pine stands and grass and forb vegetation produced by a combination of both restoration programs (LLPI‐BQI) may form habitat conditions favorable to early successional bird species and other birds, increasing avian diversity. We investigated how the LLPI and BQI programs affected taxonomic and functional diversity of birds and abundance of early successional birds (grassland and scrub/shrub species), and what environmental characteristics were associated with the diversity and abundance of birds. Our study was performed at 41 fields in Georgia, United States, during 2001–2002 by considering environmental characteristics at two spatial scales: local‐scale vegetation features and restoration program type (LLPI or LLPI‐BQI) and landscape‐scale vegetation features and landscape heterogeneity. Functional evenness, species richness, and abundance of grassland and scrub/shrub species did not show a clear association with local‐ or landscape‐scale variables. Shannon‐Wiener diversity was slightly influenced by restoration program type (local‐scale variable) with higher value at LLPI‐BQI stands than at LLPI stands despite no significant differences in local vegetation features between those stands. Functional divergence was strongly positively associated with landscape‐scale variables. That is, niche differentiation increased with increasing shrub coverage within a landscape, reducing competition between abundant bird species and others. Our results suggest that although a combination of BQI and LLPI program may have a positive effect on avian taxonomic diversity, it is important to consider shrub vegetation cover within a landscape to improve functional diversity. 相似文献
19.
The effects of habitat and timing of growing season fires on resprouting of shrubs were studied in second-growth longleaf pine savannas of the west Gulf coastal plain in the southeastern United States. Within the headwaters of three different drainages of the Calcasieu River in the Kisatchie National Forest in western Louisiana, replicated permanent transects were established that extended from xeric upland longleaf pine savannas into downslope hydric seepage savannas. All shrubs were mapped and tagged, and numbers of stems were counted prior to any fires. Replicated prescribed fires were set early (June) and late (August) during the 1990 growing season; maximum fire temperatures were measured within both upland and seepage habitats within each transect. Shrubs were relocated; stems were recensused two and twelve months after the fires. At least some shrubs of all species resprouted from underground organs; none regenerated solely from seed banks in the soil. There was no reduction in total numbers of stems one year after fires compared to before fires, either in the upland or in seepage savannas. In addition, there was no reduction in total numbers of stems one year after early or late growing season fires. Fire-related mortality was restricted to small shrubs (< 18 stems) and was not associated with high fire temperatures. The rate of resprouting varied among species and between habitats. Resprouting occurred more rapidly in seepage than upland savannas, but more resprouts were produced in upland than seepage savannas one year after fires. In contrast to other upland species, Vaccinium arboreum and V. elliottii delayed resprouting more than two months following fire. Stems of Rhus copallina and Pyrus arbutifolia, species with long rhizomes, increased more after fires in June than fires in August. We suggest that growing season fires may block further recruitment of shrubs into longleaf pine savannas, but reduction in numbers of large shrubs may require additional management. 相似文献
20.
Prescribed fire is increasingly used to inhibit woody encroachment into fire-dependent ecosystems, yet its effects on other processes influencing invasion are poorly understood. We investigated how fire influences exotic woody invasion through its effects on granivore activity, and whether these effects depend on the habitat in which seed predation takes place. We quantified seed removal for four species of exotic woody plants ( Albizia julibrissin, Elaeagnus umbellata, Melia azedarach and Triadica sebifera) in 17 sites in longleaf pine savanna that varied in time since fire (one or three growing seasons post-fire) in the sandhills region of North Carolina, USA. Within each site, we established paired plots in upland and upland-wetland ecotone communities and presented seeds in depots that allowed either arthropod, or arthropod and small vertebrate access. We found that differences in seed removal with time since fire were contingent on habitat and granivore community. In ecotones, three of four species had higher proportions of seeds removed from plots that were three growing seasons post-fire than plots one growing season post-fire, whereas only T. sebifera showed this pattern in upland habitat. Allowing vertebrate granivores access to seeds enhanced seed removal, and this effect was strongest in ecotone habitat. While granivores removed seeds of all four plant species, removal of E. umbellata was significantly higher than that of the other species, suggesting that granivores exhibited seed selection. These findings suggest that ecotone habitats in this system experience greater seed removal than upland habitats, particularly as time since fire increases, and differences are mainly due to the activity of vertebrate granivores. Such differences in seed removal, together with seed selection, may contribute to variation in exotic woody invasion of longleaf pine savannas. 相似文献
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