盲蛛血细胞分类研究

应用显微镜与相差显微镜相结合技术,对蛛形纲Ａｒａｃｈｎｉｄａ广腰亚纲Ｌａｔｉｇａｓｔｒａ、盲蛛目Ｏｐｉｌｉｏｅｓ、长奇盲蛛科Ｐｈａｌａｎｇｉｉｄａｅ的盲蛛Ｏｐｉｌｉｏ ｓｐ．血细胞进行分类,它有原血细胞浆细胞、颗粒细胞、类绛色细胞、小球细胞、类球形 细胞和足细胞等７种类型。     

大腹园蛛血细胞相差显微镜的初步研究

本文应用相差显微镜技术研究了大腹园蛛(Araneus ventricous)的血细胞。结果发现大腹园蛛血淋巴中存在五种类型的血细胞:原始血细胞、浆细胞、顆粒细胞、类绛色细胞和脂血胞。每种类型的血细胞的发生与大腹园蛛的生长发育密切相关。首次发现类绛色细胞具有脱核现象。     

海南岛的蝎子地理分布研究

蝎目是蛛形动物中较早登陆且又比较原始的类群,在蛛形动物进化历程中占重要地位,是已知最古老的陆生节肢动物之一,具有较高的药用价值和食用价值.本文对海南岛蝎子的生态地理分布进行了研究,从理论上丰富了热带地区蝎目动物的地理分布和物种丰富度等方面的内容;在实践上,拓宽了应用的领域,有利于对不同蝎目动物的资源开发和利用,同时也为保护这类古老的动物提供了依据.经调查,共发现蝎目动物2科3属3种,均为海南已有记录种类,其中钳蝎科2种,分别为等蝎属的斑等蝎和狼蝎属的细尖狼蝎;瘦尾蝎科1种,系链尾蝎属的澳链尾蝎.     

丰富多样的洞穴动物

洞穴是指人及其它生物可以进入的天然地下空间。洞穴动物可划分为经常入洞生物(Trogloxenes)、喜洞生物(Tmglobiles)和真洞穴生物(Troglobiles)等类型,以及部分偶遇动物。洞穴动物主要包括无脊椎动物中的涡虫类、腹足类、倍足类、蜘蛛类、伪蝎类、盲蛛类、甲壳类及昆虫纲的弹尾目、鞘翅目和直翅目种类;脊椎动物中的洞穴种类则     

须脚目动物系统学研究进展

须脚目是一类体型微小、形态特殊、生活于土壤或洞穴中的蛛形动物,目前全世界仅记录109种,隶属于2科8属,包括2个化石记录种。由于对该类群系统学研究比较欠缺,其系统发育地位仍不明确。基于形态学的支序系统学分析大部分支持须脚目与四肺类(蜘蛛目+无鞭目+鞭蝎目+裂盾目)聚为一支,但也有研究支持须脚目与真螨类关系较近;分子系统学方面的研究较少,其分析结果也存在分歧。目前中国须脚目动物仅有零星的报道,研究匮乏。现简要报道最近在广西发现的1种类须脚蝎属动物,分析须脚目已知科、属及其分布,比较世界各地区已知须脚目的物种数,列举须脚目研究学者及其研究区域,从经典分类学和系统发育两个方面对须脚目系统学研究的最新进展进行综述,同时给出了须脚目各科、属的中文学名翻译,以期推动中国须脚目动物系统学的开展。     

吉林省长白山区鞭突蛛属一新种（蜘蛛目：皿蛛科：微蛛亚科）

本文记述采自我国吉林省长白山区的鞭蛛属一新种,即腹斑鞭突蛛Ｔｒｉｃｈｏｎｃｕｓ ｍａｃｕｌａｔｕｓ ｓｐ．ｎｏｖ．本文测量单位为ｍｍ,模式标本均保存在白求恩医科大学生物教研室。     

蛛形纲动物含能值及其季节变动规律研究

研究干旱地区蛛形纲动物的含能值变动规律。菜纲蝎目和蜘蛛目动物含能值之间的差异不显著,平均能值为２１．３２Ｊ／ｍｇ无灰干重。但是蝎目钳蝎科（２２．１３Ｊ／ｍｇ无灰干重）、蜘蛛目的圆蛛科（２２．８２Ｊ／ｍｇ无灰干重）、巨眼蛛科（２２．６５Ｊ／ｍｇ无灰干重）、跳蛛科（２３．０８Ｊ／ｍｇ无灰干重）和狼蛛科（２１．７６Ｊ／ｍｇ无灰干重）的含能值显著地高于蟹蛛科（１９．０４Ｊ／ｍｇ无灰干重）和盗蛛科（１９．６     

琥珀的前世今生

正波罗的海琥珀制成的吊坠,其中保存有一只蜘蛛(蜘蛛目)和一只盲蛛(盲蛛目),长腿的盲蛛位于顶端琥珀是由树木的树脂在漫长的地质历史时期所变成的一种特殊的化石。琥珀是一种很轻的有机物,通常透明,呈黄色或橙色。从化学性质上来说,琥珀是一种无定形的、非晶态的混合物,其中的聚合物主要是萜类环链。我们所熟悉的一种常见的萜类就     

香港跳蛛记述

本文报道采自香港的蜘蛛目跳蛛科４新种及香港的１新记录种：铜头菱头蛛;香港菱头蛛,亲种Ｂｉａｎｏｒ ｈｏｎｇｋｏｎｇ ｓｐ．ｎｏｖ．;香港伊蛛,新种ｋｉｕｓ ｈｏｎｇｋｏｎｇｓｐ．ｎｏｖ．;香港兰纳蛛,新种Ｌａｎｇｏｎａ ｈｏｎｇｋｏｎｇ ｓｐ．ｎｏｖ．;香港盘蛛,新种Ｐａｎｃｏｒｉｕｓ ｈｏｎｇｋｏｎｇ ｓｐ．ｎｏｖ．。迄今香港已知蛛２４种。     

江西三栉毛蛛属一新种（蜘蛛目：管网蛛科）

本文记述蜘蛛目管网蛛科三栉毛蛛属一新种──江西三栉毛蛛Ｔｒｉｃａｌａｍｕｓｊｉａｎｇｘｉｅｎｓｉｓｓｐ．ｎｏｖ．。新种与ＴｒｉｃａｌａｍｕｓｐａｐｉｌｉｏｎａｃｅｕｓＷａｎｇ近似,但其体形大小和触肢器的构造与后者有明显区别。     

家蝇幼虫血细胞类型及免疫功能的初步研究

目的用不同方法观察家蝇3龄幼虫血细胞的形态,并对血细胞进行分类和免疫功能研究,为昆虫血细胞形态、分类及免疫研究提供实验依据。方法 (1)应用姬氏染色结合相差显微镜及荧光染色方法观察家蝇3龄幼虫血细胞形态,并对家蝇3龄幼虫血细胞进行分类;(2)观察家蝇3龄幼虫感染大肠杆菌后不同时间血细胞总数(THC)、各类血细胞数量(DHC)及形态的变化;(3)应用倒置显微镜观察家蝇3龄幼虫离体血细胞感染大肠杆菌后的形态变化;(4)采用酶细胞化学技术测定感染前后家蝇3龄幼虫血细胞中ACP、POD活性的变化。结果 (1)家蝇3龄幼虫血细胞可分为原血胞、浆血胞、粒血胞、珠血胞、类绛血胞5类,其中浆血胞又分为大核浆血胞和小核浆血胞两种;(2)感染后各时间组血细胞总数、浆血胞和粒血胞数量均显著升高,且浆血胞和粒血胞聚集成堆,出现细胞变形、空泡等形态变化;感染后16h、24h组的珠血胞数显著升高;原血胞和类绛血胞数量和形态无明显变化;(3)家蝇幼虫离体血细胞感染大肠杆菌后粒血胞周围见大量细菌聚集,浆血胞、粒血胞聚集成团将细菌包裹形成包囊,未见原血胞、珠血胞、类绛血胞形态的变化;(4)感染后浆血胞和粒血胞中ACP、POD的活性增强,感染前后原血胞、珠血胞、类绛血胞中均未见ACP、POD的阳性反应物。结论通过3种方法能很好地将家蝇3龄幼虫血细胞分为5类,其中浆血胞和粒血胞是家蝇幼虫参与免疫反应的主要细胞类型,珠血胞不参与感染后的早期细胞免疫反应。     

草履蚧血细胞的显微形态与超微结构观察

为研究蚧虫血淋巴中血细胞的种类及其结构特征,采用相差显微镜、荧光显微镜和扫描电镜与透射电镜技术,观察了草履蚧雌成虫血细胞的显微形态与超微结构特征.结果显示,在荧光显微镜和扫描电镜下观察识别出草履蚧血淋巴中的5种血细胞,即：原血胞、浆血胞、粒血胞、囊血胞和类绛色血细胞.在透射电镜下可见,原血胞的细胞核明显,表现出高的细胞质密度;浆血胞最典型的特征是细胞质中有大量的囊泡;粒血胞的细胞质中有发达的粗面内质网和许多玫瑰形的细颗粒.类绛色血细胞最典型的特征是有许多结晶,并在细胞质的空泡区内分布的更多.囊血胞透明性强,具有围核空间,膨大成潴泡状.     

A comparative study of mechanisms underlying digenean-snail specificity: in vitro interactions between hemocytes and digenean larvae

A panel of 4 digenetic trematode species (Echinostoma paraensei, E. trivolvis, Schistosoma mansoni, and Schistosomatium douthitti) and 5 snail species (Biomphalaria glabrata, Helisoma trivolvis, Lymnaea stagnalis, Stagnicola elodes, and Helix aspersa) was examined to determine if known patterns of host specificity could be explained by the tendency of digenean larvae to be bound by snail hemocytes, or by the ability of larvae to influence the spreading behavior of hemocytes. In short-term (1 hr) in vitro adherence assays, there was no overall pattern to suggest that sporocysts were more likely to be bound by hemocytes from incompatible than compatible snails. Compared with the other parasites, sporocysts of E. paraensei were less likely to be bound by hemocytes from any of the snail species tested. All rediae examined, including those of another species Echinoparyphium sp., were also remarkably refractory to binding by hemocytes from any of the snails. Of all the larvae examined, only sporocysts and young daughter rediae of E. paraensei caused hemocytes to round up in their presence. This was true for hemocytes from the compatible species B. glabrata and the incompatible lymnaeid species S. elodes and L. stagnalis. The patterns of host specificity shown by this particular panel of parasites and snails were not predicted by either the extent of hemocyte adherence to digenean larvae or by the ability of larvae to affect hemocyte spreading behavior. The results of this study suggest that a role for hemocytes, although likely, may require different assays, possibly of a more prolonged nature, for its detection. Also, different parasite species (notably E. paraensei) and intramolluscan stages have distinctive interactions with host hemocytes, suggesting that the determinants of specificity vary with the host-parasite combination, and with the parasite life cycle stage.     

Hemocytes from neonates of Biomphalaria glabrata are functionally less mature than those from adult snails

Gastropod molluscs, which serve as obligatory intermediate hosts for digenetic trematodes, possess an internal defense system (IDS) consisting of phagocytic hemocytes and plasma factors. This IDS is responsible for resistance to infection with larval trematodes, which are encapsulated and killed by hemocytes in incompatible snails. Like other physiological systems, the IDS probably undergoes maturation during early stages of life, and the relatively undeveloped state of the IDS in young snails has been hypothesized to be a factor in their increased susceptibility to infection with larval trematodes. In this study, hemocytes were examined in the BS-90 laboratory strain of Biomphalaria glabrata that is resistant to infection with Schistosoma mansoni as adults but susceptible to infection as neonates. Compared with hemocytes from adults, hemocytes from neonates had a smaller perimeter and lower intrinsic directional motility on glass microscope slides. Additionally, in vitro assays showed a lower association with fucoidan-linked polystyrene beads and less ability to produce superoxide anion in hemocytes from neonates compared to hemocytes from adults. These results support the hypothesis that the gastropod IDS undergoes maturation during growth. However, whether the observed differences between hemocytes of neonatal and adult BS-90 snails play a role in the susceptibility of the former and resistance of the latter to infection with S. mansoni is not known.     

Fine structure and classification of shrimp hemocytes

The structure of hemocytes from two species of penaeid shrimp was examined by light and electron (TEM) microscopy. Hemocytes from the two species are indistinguishable and are classified as either agranular, small-granule, or large-granule hemocytes. Agranular hemocytes are the smallest of the hemocytes, lack granules, compose only 5–10% of the circulating hemocytes, and are nonrefractile when examined by light microscopy. Small-granule hemocytes are the most abundant type of hemocyte (75% of all hemocytes), appear nonrefractile, and contain a variable number (1–40) of granules (0.4 μm diameter). Large-granule hemocytes compose 10–20% of the hemocytes. They are filled with granules (0.8 μm in diameter) that are highly refractile when examined by light microscopy and are electron-dense when examined by TEM. Our classification scheme is based solely on the absence or presence and relative size of granules. Features used by other researchers, such as cell size, shape, and staining properties, were not used because these features are subtle and/or subjective. The proposed classification is compared with schemes developed for other decapods, and its usefulness and limitations are discussed. This scheme will serve as a basis for further studies on the maturation and physiological function(s) or crustacean hemocytes.     

家蚕拟浆细胞具有吞噬功能

目的：通过观察家蚕Bombyx mori吞噬细胞的微细结构,来确定拟绛色细胞是否也具有吞噬功能。方法：用荧光小球微量注射家蚕pnd pS品系的幼虫,经荧光染色剂丫啶橙和碘化丙啶染色循环血细胞后,在荧光显微镜下观察并扫描拍摄。结果：观察发现除颗粒细胞和浆血细胞外,一些原血球细胞（血干细胞）和拟绛色细胞（多酚氧化酶）也能吞噬荧光小球。在拟绛色细胞里还发现许多和颗粒细胞一样的能被丫啶橙染色的颗粒。尽管在小球细胞中没有发现被吞噬的荧光小球,但该类血球有比较多的能被丫啶橙染色的大颗粒,这表明它们可能是已经被吞噬的凋亡小体。结论：除颗粒细胞和浆血细胞外,一些原血球细胞和拟绛色细胞也能吞噬荧光小球。说明拟绛色细胞也具有吞噬功能。     

Evidence for an H-Y Crossreactive Antigen in Invertebrates

A sex specific antigen which crossreacts with the mammalian H-Y antigen has been identified on the cell surface of hemocytes from the lobster ( Homarus americanus ) and the gonadal cells of three insect species. The hemocytes from the male lobster, the testicular cells from the male beetle ( P. cornutus ), and the ovarian cells from two Orthopteran species ( L. maderae and D. punctata ) specifically absorbed H-Y antibodies. The specificity of H-Y antibody absorptions by cells from only one sex, suggest that an ancestral H-Y-like antigen may be present in invertebrates which could be engaged in sexual (cellular) recognition events.     

红褐斑腿蝗血细胞的形态与分类

利用光学显微镜和显微数码拍照系统,对红褐斑腿蝗Catantops pinguis(Stal)血细胞的形态进行观察和分类。结果在红褐斑腿蝗血淋巴中观察到5种血细胞,分别是原血胞、浆血胞、粒血胞、珠血胞和囊血胞。原血胞为小型圆形细胞,边缘圆滑、清晰,核质比例很大。粒血胞多为中型,形状不规则,边缘凹突不平,内含较大的异质性溶酶体颗粒。浆血胞多为中型,刚离体时形状较规则,常呈圆形、卵圆形。浆血胞内缺乏大的颗粒,细胞核大而圆形,细胞质内具许多小型颗粒状物质。浆血胞离体后形状变化较多,常发展出伪足,呈丝状、短芒状、钩状或片状伪足。珠血胞多为大、中型,外形大体呈圆形,但边缘由于大小不等的珠形内含物突出,呈花瓣状。囊血胞多为中型,圆形或椭圆形,细胞质内具有大小不一的带有折光性的颗粒或块状物,细胞边缘比较光滑。     

Production of reactive oxygen species by hemocytes from the cattle tick Boophilus microplus.

We have investigated the phagocytic activity and the production of reactive oxygen species (ROS) by hemocytes from the cattle tick Boophilus microplus. Two main types of hemocytes were detected in tick hemolymph: plasmatocytes and granulocytes. The plasmocytes were the most abundant cells, being responsible for the in vivo phagocytosis of yeast. ROS production was evaluated by luminol-amplified luminescence and phenol red oxidation. The luminescence increased when hemocytes were incubated with bacteria, zymosan, or phorbol 12-miristate 13-acetate (PMA). The luminescence was inhibited by superoxide dismutase and catalase, which are antioxidant enzymes that remove superoxide and hydrogen peroxide, respectively. The phenol red oxidation assay also showed an increase in the level of hydrogen peroxide produced by hemocytes stimulated with bacteria and PMA. Taken all together, our data indicate that tick hemocytes are able to produce ROS during the phagocytic process similarly to vertebrate phagocytes.