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1.
The binding of lipoproteins to the oocyte plasma membrane of the domestic fowl (Gallus domesticus) was examined by electron microscopy in preparations of the ovarian follicle in the main phase of yolk formation. Numerous particles, 26 nm in diameter, were present on the untreated membrane. They were dissociated from the membrane by incubation at 4 °C in buffer at pH 6.2 and with heparin at pH 7.4. Added calcium was not required for binding, though the number of bound particles was reduced by treatment with EDTA. Very low density (VLD) lipoproteins from laying hen's plasma were found to bind to the denuded membrane and to correspond in size to the native particles. The results suggest that the binding characteristics are similar in quality to those determined for the binding of low density (LD) lipoproteins to mammalian cells. The oocytes, however, bound 100-fold more particles per unit length of membrane. VLD and LD lipoproteins from immature hens also adhered to the denuded membrane, although their apoprotein composition was very different from that of laying hen VLD lipoproteins. LD lipoproteins from immature hens and VLD lipoproteins from laying hens both contained apo-B, which formed about 80 and 35%, respectively, of the total apolipoproteins. Apo-VLDL-II is the other major apoprotein identified in laying-hen VLD lipoproteins. Apo-VLDL-II was not positively identified as a component of immature-hen LD lipoproteins and could only have been present as a minor component. Despite their great difference in apo-VLDL-II content, immature-hen LD lipoproteins and laying-hen VLD lipoproteins showed similar dissociation constants for binding to the oocyte plasma membrane. This evidence strongly suggests that the cell surface receptors recognize the B apoprotein of avian VLD lipoproteins. 相似文献
2.
P J Babin 《The Journal of biological chemistry》1987,262(9):4290-4296
The apolipoproteins of trout plasma lipoproteins have been characterized by sodium dodecyl sulfate-glycerol polyacrylamide gel electrophoresis. The high density lipoproteins (HDL) (1.085 less than d less than 1.21 g/ml) contain four apolipoproteins, two major species with Mr 25,000 (apoA-I-like) and Mr 13,000 (apoA-II-like) and two minor species (Mr 55,000 and 40,500). The very low density (d less than 1.015 g/ml) and low density lipoproteins (1.015 less than d less than 1.085 g/ml) contain two high Mr apolipoproteins (apoB-like) with Mr 260,000 and 240,000 (the smaller is the preponderant species in low density lipoproteins), as well as a third apolipoprotein with Mr 76,000. Type A apolipoproteins are present in the very low density lipoproteins, as are a group of apolipoproteins with Mr 9,000-11,000 (apoC-like). Egg yolk proteins appear in the plasma of females about 30 days after natural ovulation or after that induced by salmon gonadotropin and during massive intraovarian atresias, either spontaneous or induced by 17 alpha,20 beta-dihydroxy-4-pregnen-3-one. Two egg yolk proteins intimately associated with HDL have been identified. They may account for as much as 35% of total plasma proteins. Lipovitellin (Mr 112,000) is composed of two subunits in a 1:1 molar ratio (lipovitellin 1 with Mr 92,000 and lipovitellin 2 with Mr 20,000) and is present as a dimer with another yolk protein (Mr 10,000). These results show that resorption of the yolk during follicular atresia in an oviparous vertebrate is correlated with the presence of egg yolk proteins combined with HDL in the plasma. 相似文献
3.
S. Mahadevan 《Journal of biosciences》1987,11(1-4):299-309
Lipid thermal transition patterns of the very low density lipoproteins in native and variously treated egg yolk plasma and
extracted total very low density lipoproteins lipids have been recorded by differential scanning calorimetry in the temperature
range 220–300 K, after lowering the freeze endotherm of free water in the sample with ethylene glycol.
Three distinguishable patterns of lipid endotherms, designated types 1, 2 and 3 were obtained, respectively, from (i) native
very low density lipoproteins in egg yolk plasma, (ii) freeze damaged very low density lipoproteins in gelled egg yolk plasma
and (iii) extracted total lipids of very low density lipoproteins dispersed in water. Protein-depleted ‘lipid core’ particles
of very low density lipoproteins obtained by exhaustive proteolysis of egg yolk plasma gave type 2 lipid transition pattern
suggesting similarities in its lipid association with that of the freeze damaged very low density lipoproteins. Freezing the
‘lipid cores’ of very low density lipoproteins led to phase separation and gave type 3 lipid transition pattern of water-dispersed,
phase-separated total very low density lipoprotein lipids. Relative heat uptake of native very low density lipoproteins in
egg yolk plasma was about 15% lower than the freeze damaged sample or of the extracted total lipids.
Treatments which prevented aggregation and gelation of very low density lipoproteins in egg yolk plasma during frozen storage,
namely with additives such as glycerol or NaCl, gave subsequent lipid transition pattern intermediate between type 1 and 2,
indicating that while very low density lipoprotein aggregation is prevented, additives do not altogether prevent changes in
lipid association in these particles. 相似文献
4.
The laying hen represents a physiological model in which the mechanisms of action of estrogens on lipid transport can be evaluated. The plasma lipoproteins in the laying hen were subfractionated into discrete particle species by isopycnic density gradient ultracentrifugation and the physicochemical properties and apolipoprotein contents of individual subfractions evaluated. The qualitative and quantitative aspects of this estrogen-specific profile were then compared to those of the immature chicken. As observed earlier, estrogens induced dramatic elevation in very-low-density lipoproteins (VLDL) (up to 900 mg/dl). Indeed, triglyceride-rich lipoproteins with densities up to 1.035 g/ml, i.e. VLDL and their remnants, behaved as a continuum which displayed little variation in size (20.5-21 nm), electrophoretic mobility (beta-like) and apolipoprotein content; apo B-100 (540 kDa) predominated while apo A-I (27 kDa), apo VLDL-II (19 kDa) and an apo-C-like protein (13 kDa) were present as minor components. The typical high-density lipoproteins (HDL) in the immature chicken were replaced by a lipoprotein population whose physicochemical properties were quite distinct. Thus these particles were distributed as a single, asymmetric peak over the density range 1.030-1.158 g/ml, a wide interval which overlapped that of apo-B-rich particles at its lower limit. The rho 1.030-1.158 g/ml lipoproteins were present at concentrations (approximately equal to 200 mg/dl) some twofold to threefold lower than those of HDL in immature birds. Furthermore, they displayed physical and chemical properties in common with both low-density lipoproteins (LDL) and HDL and were LDL-like in exhibiting beta mobility but HDL-like in size (9-15 nm diameter). Their protein moiety was also HDL-like in its predominant content of apo A-I; small amounts of apo VLDL-II and the apo-C-like protein were also detected. Substantial amounts of lipid were found at rho greater than 1.195 g/ml: such substances are absent in the immature chicken and may reflect the presence of vitellogenins. The hyperestrogenic state in the laying hen is therefore associated with major modifications in lipoprotein and apolipoprotein profile. Such modifications may be of relevance to clinical disorders involving estrogen-induced hyperlipidemia. 相似文献
5.
At onset of egg-laying in the chicken, plasma levels of apolipoprotein VLDL-II (apoII) increase dramatically, suggesting a function of apoII in yolk deposition of triglyceride-rich lipoproteins. Thus, the possibility that this female-specific homodimeric protein (Mr of subunit, 9500) is recognized by the oocyte receptor for low and very low density lipoproteins was investigated. ApoII was purified from very low density lipoproteins by a novel, rapid procedure and reconstituted with egg phosphatidylcholine (PC) by detergent-dialysis. The resulting discoidal apoII/PC lipoprotein particles contained 3 mg of apoII per mg of PC and had a buoyant density of 1.062 g/ml. The ability of apoII/PC, as well as of physiological particles containing apoII but devoid of apolipoprotein B (apoB), namely high density lipoproteins (HDL) from laying hens, to interact with the oocyte receptor was tested. Both of these ligands failed to show saturable high affinity binding, in contrast to the apoB-containing ligands, low and very low density lipoproteins. Furthermore, neither laying-hen HDL which contain apoII and apoA-I nor apoII/PC were able to displace receptor-bound apoB-containing lipoproteins, as shown in competitive binding assays as well as by ligand blotting. Thus, we conclude that apoB, but not apoII, participates in binding and uptake of very low density lipoproteins via receptor-mediated endocytosis by growing chicken oocytes. 相似文献
6.
High resolution nuclear magnetic resonance spectra of native or protease-treated hen’s egg yolk plasma (very low density lipoproteins)
were taken either in water or deuterated water; the protease-treated samples showed a sharpening of choline methyl proton
signal of phospholipid, indicating the hindrance of the choline head-group rotation by the phospholipids in the native very
low density lipoproteins. With both native and the protease-treated egg yolk plasma, elevated temperatue increased the signal
intensity and produced line-sharpening of Q choline methyl protons and the — CH2-C-protons of the methylene group adjacent to the carboxyl group of esterified fatty acids, indicating prior restriction of
mobility of these groups. Total extracted lipids of egg yolk plasma containing traces of chloroform, methanol and water (which
keep the sample in one phase) also gave similar temperature dependence. Addition of water to the same sample and sonication
resulted in the loss of temperature dependence. Frozen and thawed protease-treated egg yolk plasma also behaved in a similar
manner. The absence of temperature dependence in these latter two samples is believed to be due to formation of bilayers of
phospholipids following phase separation of triglycerides and phospholipids. The results support a model in which the lipoprotein
particles of the egg yolk plasma have a lipid-core structure containing triglycerides in the centre with a monomolecular layer
of lecithin at the surface, the polar heads of which are surrounded by proteins.
Contribution No. 149 from the Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012. 相似文献
7.
Isolation and characterization of the major apolipoprotein from chicken high density lipoproteins. 总被引:3,自引:0,他引:3
High density lipoproteins were isolated from plasma of white Leghorn hens by ultracentrifugal flotation between densities 1.063 and 1.210 g/ml. After delipidation, the lipid-free proteins were fractionated by chromatography on Sephadex G-150 in urea; one major apolipoprotein was isolated and characterized. From its chemical, physical and immunochemical properties, the major apoprotein from hen high-density lipoproteins has characteristics similar to the major apoprotein of human high density lipoproteins, apoA-I. Thus the hen protein has been designated hen apoA-I. Hen apoA-I has a molecular weight of approximately 28 000 as determined by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Its calculated molecular weight from its 234 constituent amino acids is 26 674. Hen apoA-I differed from its human counterpart by containing isoleucine. Treatment of hen apoA-I with carboxypeptidase A yielded a COOH-terminal sequence of Leu-Val-Ala-Gln. Automatic Edman degradation of the apoprotein gave an NH2-terminal sequence of Asp-Glu-Pro-Gln-Pro-Glu-Leu. Hen apoA-I had a circular dichroic spectrum typical of alpha-helical structures; the calculated helicity was 90%. Goat antisera prepared to hen apoA-I formed precipitin lines of complete identity to the hen apoprotein but lines of only partial identity to human apoA-I. These studies show that the major apoprotein from hen and human high-density lipoproteins have similar properties to each other suggesting a common physiologic function. 相似文献
8.
《Biochimica et Biophysica Acta.Protein Structure》1976,420(2):342-349
High density lipoproteins were isolated from plasma of white Leghorn hens by ultracentrifugal flotation between densities 1.063 and 1.210 g/ml. After delipidation, the lipid-free proteins were fractionated by chromatography on Sephadex G-150 in urea; one major apolipoprotein was isolated and characterized. From its chemical, physical and immunochemical properties, the major apoprotein from hen high-density lipoproteins has characteristics similar to the major apoprotein of human high density lipoproteins, apoA-I. Thus the hen protein has been designated hen apoA-I.Hen apoA-I has a molecular weight of approximately 28 000 as determined by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Its calculated molecular weight from its 234 constituent amino acids is 26 674. Hen apoA-I differed from its human counterpart by containing isoleucine. Treatment of hen apoA-I with carboxypeptidase A yielded a COOH-terminal sequence of Leu-Val-Ala-Gln. Automatic Edman degradation of the apoprotein gave an NH2-terminal sequence of Asp-Glu-Pro-Gln-Pro-Glu-Leu. Hen apoA-I had a circular dichroic spectrum typical of α-helical structures; the calculated helicity was 90%. Goat antisera prepared to hen apoA-I formed precipitin lines of complete identity to the hen apoprotein but lines of only partial identity to human apoA-I. These studies show that the major apoprotein from hen and human high-density lipoproteins have similar properties to each other suggesting a common physiologic function. 相似文献
9.
R J Evans D H Bauer S L Bandemer S B Vaghefi C J Flegal 《Archives of biochemistry and biophysics》1973,154(2):493-500
Egg yolk very low density lipoprotein contained on the average 75% of lipid which could be extracted by ether and 25% of a residual lipoprotein, the classical lipovitellenin. The ether-extracted lipid was composed of 75% triglycerides, 7% sterols, 2% mono- and diglycerides, and 16% phospholipids. Lipovitellenin contained 48% lipid composed of 87% phospholipids, 11% triglycerides, and 2% sterols. The protein vitellenin was composed for the most part of units of 74,000 and 270,000 daltons molecular weight.Egg yolk very low density lipoprotein is polydisperse. Preparative ultracentrifugation separated it into six fractions of different average molecular size, and gel chromatography separated it into five. The fractions of larger molecular size contained more lipid and triglyceride than did the fractions of smaller molecular size. The proteins of the fractions appeared to be similar.Egg yolk very low density lipoproteins appear to be a series of molecules composed of cores of lipid of varying sizes with each core surrounded by a layer of lipovitellenin, which is composed principally of glycoprotein and phospholipid. 相似文献
10.
Proteolysis of apoprotein B during the transfer of very low density lipoprotein from hens' blood to egg yolk 总被引:2,自引:0,他引:2
We report an example of the enzymic cleavage of an apoprotein B (apoB), the main apoprotein in the very low density lipoprotein (VLDL) of laying hens' blood, in a normal biological process, the formation of egg yolk. Plasma VLDL was labeled in vivo with 3H-amino acids, isolated by centrifuging, and injected into another laying hen. Yolk VLDL was isolated and its apoproteins were separated. ApoB was not detected in this lipoprotein. Most of the label originally in apoB was distributed among four smaller yolk apoproteins, apovitellenins III to VI, which are a large proportion of the apoproteins of VLDL in yolk. This distribution of 3H suggested that 80% of apoB was cleaved at three places. One yolk apoprotein, apovitellenin II, was not labeled, indicating that it did not originate from an apoprotein in plasma VLDL. The site for cleavage of apoB in the ovarian tissue has not been determined, but cleavage may occur during receptor-mediated endocytosis. The pattern of cleavage of apoB during transfer to yolk was not imitated by some known proteolytic enzymes. 相似文献
11.
Changes of the estradiol and lipid concentrations in hen serum, and those in the fatty acids of hen and cockerel livers were studied during the maturing period. The concentrations of estradiol and lipid in serum reached a maximum level a few days before the onset of laying, and then fell. A marked increase in oleate, and decreases in stearate and arachidonate were observed in both the liver and serum lipids of the hen when reaching laying. The fatty acid compositions of the serum and liver of the hen at 145 and 167 days of age were close to those of yolk lipid. In the cockerel, high contents of stearate and arachidonate and a low content of oleate were observed, and the fatty acid composition did not change appreciably with age. On estrogenization of the male chick, the fatty acid cmposition of the liver lipid was similar to that of the laying hen liver. The ratio of the unsaturated fatty acid/saturated fatty acid in the liver of hen increased when reaching laying, the value at 167 days old being close to that of yolk lipid. This ratio in the liver lipid of cockerel did not change significantly throughout the experimental period. 相似文献
12.
We have compared the amino acid sequences of two low-molecular-weight avian apoproteins: apoVLDL-II from very low-density lipoproteins of hen plasma and apovitellenin I from hen egg yolk. The sequence of White Leghorn apoVLDL-II was derived from the nucleotide sequence of cloned apoVLDL-II DNA (Chan et al., 1980). The sequenator was used to determine the amino acid sequence of apovitellinin I from two breeds of hen (White Leghorn and Australorp). The sequences from the two breeds were not only identical, but they also completely matched the predicted sequence derived from the apoVLDL-II DNA sequence. The identity reported here establishes that this protein is transported intact from the blood to the egg yolk. 相似文献
13.
Production and fates of transosomes (sacs of ribosomes made in the follicular cells of an ovarian follicle and subsequently passed to the cytoplasms of the oocyte) have been studied by electron microscopy in ovaries of young chicks, a testosterone-treated hen, aged hens which had ceased laying eggs and a "non-layer" mutant. Study was also made of "primitive yolk" (vacuoles present in both follicular cells and ooplasms of small follicles of normally laying hens). It was found that both transosomes and vacuoles of primitive yolk were present in small oocytes of young chicks, and "non-layer" mutants. However, the transosomes deep within the ooplasms were present within lysosomal vesicles in both of these instances and the vacuoles containing primitive yolk were patently abnormal in the "non-layer" mutant. Very few transosomes or primitive yolk vacuoles were present within the ooplasms of follicles from a testosterone-treated hen or from those of aged hens which were no longer laying. In both of these latter cases such bodies were present in the follicular cells. However, many transosomes were seen to be in the process of being lysed within the cytoplasms of these follicular cells. 相似文献
14.
Williams TD Ames CE Kiparissis Y Wynne-Edwards KE 《Proceedings. Biological sciences / The Royal Society》2005,272(1559):173-177
We investigated the relationship between plasma and yolk oestrogens in laying female zebra finches (Taeniopygia guttata) by manipulating plasma oestradiol (E2) levels, via injection of oestradiol-17beta, in a sequence-specific manner to maintain chronically high plasma levels for later-developing eggs (contrasting with the endogenous pattern of decreasing plasma E2 concentrations during laying). We report systematic variation in yolk oestrogen concentrations, in relation to laying sequence, similar to that widely reported for androgenic steroids. In sham-manipulated females, yolk E2 concentrations decreased with laying sequence. However, in E2-treated females plasma E2 levels were higher during the period of rapid yolk development of later-laid eggs, compared with control females. As a consequence, we reversed the laying-sequence-specific pattern of yolk E2: in E2-treated females, yolk E2 concentrations increased with laying-sequence. In general therefore, yolk E2 levels were a direct reflection of plasma E2 levels. However, in control females there was some inter-individual variability in the endogenous pattern of plasma E2 levels through the laying cycle which could generate variation in sequence-specific patterns of yolk hormone levels even if these primarily reflect circulating steroid levels. 相似文献
15.
Carotenoids in eggs and plasma of red-legged partridges: effects of diet and reproductive output 总被引:2,自引:0,他引:2
Bortolotti GR Negro JJ Surai PF Prieto P 《Physiological and biochemical zoology : PBZ》2003,76(3):367-374
Carotenoids are important dietary constituents in birds. They serve as pigments and play numerous physiological roles in both the laying hen and developing embryo. However, factors determining the absorption of carotenoids and their allocation to different functions are numerous and complex, and causal relationships are generally poorly known. Our objective was to determine the degree to which carotenoid levels in egg yolks and the plasma of hens were influenced by differences in diet and reproductive output in captive red-legged partridges. Carotenoid concentrations were measured by high performance liquid chromatography in two feeds (high and low carotenoid content) and in yolks and plasma of hens near the start and end of laying. Early in the laying season, plasma and yolk carotenoids varied with diet and were correlated with one another. Late in the season, a dietary effect was evident only for yolks, and there was no relationship between plasma and egg levels of individual hens. However, plasma carotenoids at the end of laying were strongly correlated with the number of eggs that had been laid. Dietary availability, although important, could explain some variation in carotenoid levels in plasma and egg yolks only in the context of reproductive history. 相似文献
16.
The hyperlipoproteinemia observed after ovariectomy in rats was previously shown to be associated with increased concentrations of cholesterol, triglycerides, and apolipoproteins B, E, and C. In the present study, it was shown that increases in low density lipoproteins and high density lipoproteins were almost entirely responsible for the changes in plasma lipids and apolipoproteins after ovariectomy. The size of the low density lipoproteins and high density lipoproteins isolated from the plasma of ovariectomized rats as determined by agarose chromatography appeared to be somewhat different from that of control rats. Specifically, the apolipoprotein B appeared to be associated with somewhat smaller particles, whereas the apolipoprotein E from those rats appeared to be associated with larger particles than that of control rats. To determine the mechanism for the increased plasma low density lipoproteins, apolipoprotein B pool sizes and turnover rates were calculated and compared. In addition to an increased mass of low density lipoproteins in ovariectomized rats, the turnover rate of low density lipoproteins was increased almost twofold, indicating an increased low density lipoprotein synthesis and catabolism in those animals. We postulate that the increased low density lipoprotein levels of ovariectomized rats are due to an initial increased production of low density lipoproteins, followed by an enhanced catabolism of low density lipoproteins to establish a steady state at higher plasma low density lipoprotein concentrations. 相似文献
17.
Physiological fatty liver and hyperlipemia in the fetal guinea pig: chemical and ultrastructural characterization 总被引:3,自引:0,他引:3
During its prolonged period of gestation, the fetal guinea pig gradually develops a striking hyperlipemia (plasma triglycerides ca. 500-1500 mg/dl) and fatty liver (hepatic triglycerides ca. 25% of wet weight). The parenchymal cells of the liver contain not only many fat droplets in the cytoplasm, but also large numbers of osmiophilic particles, interpreted as precursors of plasma lipoproteins, within profiles of the cisternae and secretory vesicles of the Golgi apparatus. Similar particles are found in intercellular spaces, in the space of Disse, and in the hepatic sinusoids. Near the end of gestation, these particles enlarge to the size range characteristic of chylomicrons secreted from the intestinal mucosa after ingestion of fat. At the same time, the hyperlipemia increases and is characterized by the accumulation of particles resembling chylomicrons morphologically and chemically. The results are interpreted as evidence of intense hepatic synthesis and secretion of very low density lipoproteins which may be related to the extensive transplacental transport of free fatty acids known to occur in this species. After birth, the hyperlipemia subsides rapidly and the hepatic steatosis more gradually. The blood plasma of the guinea pig fetus also contains moderate amounts of low density and high density lipoproteins. The latter decrease to barely detectable levels during the first 2 wk of postnatal life. Comparably low levels of high density lipoproteins are found in nonpregnant and pregnant adults. 相似文献
18.
Plasma zinc as an index of vitellogenin production and reproductive status in the domestic fowl. 总被引:2,自引:0,他引:2
M A Mitchell A J Carlisle 《Comparative biochemistry and physiology. A, Comparative physiology》1991,100(3):719-724
1. A technique is described for the accurate determination of circulating vitellogenin concentrations by measurement of plasma zinc concentrations before and after selective precipitation of lipoproteins in the domestic fowl. 2. Vitellogenin zinc concentrations exhibit a high degree of correlation with those of another major egg yolk precursor, very low density lipoprotein (VLDL) in immature female birds, during developing and maximum egg production in mature birds and following cessation of egg laying. 3. Mature female patterns of plasma vitellogenin and VLDL were induced by injection of oestradiol 17 beta (5 mg/kg body weight per day for 3 days) into adult cockerels. 4. It is suggested that measurement of plasma zinc provides a simple and accurate technique for the estimation of vitellogenin production and reproductive status in the domestic fowl and that this may be applied to other oviparous vertebrates. 相似文献
19.
Apolipoprotein VLDL-II inhibits lipolysis of triglyceride-rich lipoproteins in the laying hen 总被引:3,自引:0,他引:3
In the laying hen, very low density lipoprotein (VLDL) particles contain large amounts of apolipoprotein (apo)-VLDL-II in addition to apoB. These triglyceride-rich lipoproteins are transported from the liver primarily to growing oocytes. Since no appreciable hydrolysis of triglyceride occurs during this transport, we have investigated the possibility that apoVLDL-II functions as an inhibitor of lipoprotein lipase (LPL). The presence of LPL in chicken follicular granulosa cells was demonstrated by immunoblotting, and LPL activity with the usual in vitro characteristics could be measured in cultured granulosa cell extracts. ApoVLDL-II inhibited LPL activity in these extracts as well as in the post-heparin medium of rat cardiac myocytes. Half-maximal inhibition in both systems occurred at 40 micrograms/ml, a concentration that is one-tenth of the circulating apoVLDL-II in the laying hen. Much less inhibition was observed with reduced and alkylated apoVLDL-II and with apoA-I. We conclude that the presence of apoVLDL-II on laying hen VLDL ensures efficient delivery of triglyceride to the oocyte for subsequent use as energy source by the embryo. 相似文献
20.
The hydrolysis of glycerophospholipids in very low density lipoprotein by enzyme(s) released into circulation after the injection of heparin to rats was studied. [32P]Lysolecithin was formed rapidly from [32P]lecithin when very low density lipoprotein, labeled biosynthetically with 32P, was incubated with postheparin plasma. The [32P]lysolecithin was associated with the plasma protein fraction of density greater than 1.21 g/ml, whereas [32P]lecithin exchanged between very low and high density lipoproteins. Inhibition of the plasma lecithin: cholesterol acyl transferase activity did not change the excess [32P]lysolecithin formation in postheparin plasma, and only a negligible amount of radioactivity was associated with blood cells when the incubation was repeated in whole blood. Analysis of the results has demonstrated that phospholipids are removed from VLDL by two pathways: hydrolysis of glycerophospholipids by the heparin-releasable phospholipase activity (greater than50%) and transfer to high density lipoproteins (less than50%). The tissue origin of the postheparin phospholipase was studied in plasma obtained from intact rats and supradiaphragmatic rats using specific inhibitors of the extrahepatic lipase system (protamine sulfate and 0.5 M NaCl). The phospholipase activity could be ascribed to both the hepatic and extrahepatic lipase systems. It is concluded that hydrolysis of glycerophospholipids is the major mechanism responsible for the removal of phospholipids from very low density lipoprotein during the degradation of the lipoprotein. It is suggested that phospholipid hydrolysis occurs concomitantly with triglyceride hydrolysis, predominantly in extrahepatic tissues. 相似文献