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1.
The growth of Acinetobacter species HO1-N on a homologous series of dialkyl ethers yielded characteristic cellular and extracellular ether fatty acids. Microbial growth on diheptyl ether resulted in the appearance of 7-n-heptoxy-1-n-heptanoic acid as a cellular fatty acid and 2-n-heptoxy-1-acetic acid as the sole extracellular fatty acid. The oxidation of dinonyl ether and didecyl ether by Acinetobacter resulted in the extracellular accumulation of 2-n-nonoxy-acetic acid and 2-n-decoxy-1-acetic acid, respectively. The 16-carbon ether fatty acid, 6-n-decoxy-1-n-hexanoic acid, was identified as a major cellular fatty acid in didecyl ether-grown cells. The extracellular ether fatty acids accumulated in an inverse relationship to the disappearance of the dialkyl ether and appeared to represent end products of metabolism. The carbon and energy required for cellular growth and metabolism resided in the terminal 5-carbons of diheptyl ether, 7-carbons of dinonyl ether and 8-carbons of didecyl ether. Glutarate, adipate, pimelate and suberate were identified from cells grown at the expense of diheptyl, dioctyl, dinonyl and didecyl ether, respectively, suggesting a role for dibasic acids as metabolic intermediates. A new and novel mechanism for the metabolism of symmetrical dialkyl ethers is suggested. Terminal methyl group oxidation of the dialkyl ether results in the formation of an alkoxy-fatty acid followed by an internal carbon-carbon scission reaction 2-carbons removed from the oxygen atom. The resulting endproducts are alkoxyacetic acid and the corresponding dibasic acid.Non-Standard Abbreviations TLC
Thin Layer Chromatography
- PS-DEGS · PS
Diethylene glycol succinate
- DHE
Diheptyl ether
- DOE
Dioctyl ether
- DNE
Dionyl ether
- DDE
Didecyl ether 相似文献
2.
Dainiak MB Izumrudov VA Muronetz VI Galaev IYu Mattiasson B 《Journal of molecular recognition : JMR》1998,11(1-6):25-27
The simplified model of chaperone action when the inactive misfolded forms are removed from the reaction media preventing aggregation was developed using antibodies in combination with polyelectrolyte complexes. The antibodies, which bind specifically inactive dimers of glyceraldehyde-3-phosphate dehydrogenase but not native tetramers, were coupled covalently to poly(methacrylic acid). The treatment of inactivated GAPDH with this conjugate followed by its precipitation after equimolar addition of polycation, poly-(N-ethyl-4-vinylpyridinium bromide), resulted in a significant increase in the specific activity of the enzyme. 相似文献
3.
D. Chappard C. Alexandre S. Palle J. P. Month ard G. Riffat 《Biotechnic & histochemistry》1986,61(3):185-186
The technical note from Bernier et al. (1984) presents additional observations on our procedure for purifying glycol methacrylate (GMA), a hydrophylic resin (Chappard et al. 1982). It is becoming increasingly popular and widely used as an embedding medium for light microscopic studies. GMA is prepared by esterification of methacrylic acid (MA), but about 1% of free unreacted MA remains in the monomer. MA can copolymerize with GMA and it also binds strongly to thiazin and other basic dyes (Tipett and O'Brien 1975) so as an undesirable impurity it must be removed. 相似文献
4.
Saboktakin MR Tabatabaie RM Maharramov A Ramazanov MA 《International journal of biological macromolecules》2011,48(3):403-407
The main objective of this study was to develop a local, oral mucoadhesive metronidazole benzoate (MET) delivery system that can be applied and removed by the patient for the treatment of periodontal diseases. The results of present study revealed that the retention time of MET at its absorption site could be increased by formulating it into nanoparticles using thiolated chitosan (TCS)-poly(methacrylic acid) (PMAA). The nanoparticles of MET prepared from TCS-PMAA may represent a useful approach for targeting its release at its site of absorption, sustaining its release and improving its oral availability. 相似文献
5.
Arnold De Loof Kornath Madhavan J.E. Girard Howard A. Schneiderman J. Meinwald 《Journal of insect physiology》1976,22(6):829-832
An improved procedure for extraction of juvenile hormones (JH) from large quantities of insects is described. It consists of the following steps. The lyophilised insects are homogenized in cold methanol and, after storage overnight, the debris is removed by filtration and the methanol is evaporated. For the next two steps the residue is dissolved in ether and by adding either methanol in the first step, or acetone in the second step, a precipitate is formed which is removed by filtration. With this method, 2 to 7 times less lipids are extracted than with usual methods involving ether or ethyl acetate, but more than 99.5 per cent of the JH is extracted at least in H. cecropia. The JH activity of the extracts is at least 10 times higher than extracts prepared with ether or ethyl acetate alone. 相似文献
6.
Ciardelli G Cioni B Cristallini C Barbani N Silvestri D Giusti P 《Biosensors & bioelectronics》2004,20(6):1083-1090
Cross-linked poly(methylmethacrylate-co-methacrylic acid) nanospheres were imprinted with theophylline through template radical polymerisation in diluted acetonitrile solution. This study will focus on the effect of functional monomer nature used (methylmethacrylate and/or methacrylic acid) in the recognition and in the release of template in order to develop a material with combined properties of drug delivery and rebinding for clinical applications. After template extraction the nanospheres showed satisfactory recognition properties (up to 1mg template/g of polymer). Moreover polymers prepared selectively removed theophylline with a theophylline rebinding of 5.1 times higher than that of caffeine, a compound of similar structure. Drug release properties were also satisfactory (up to 95% of loaded theophylline in 7 days). 相似文献
7.
Poly(3-hydroxybutyrate) is nontoxic and biodegradable, with good biocompatibility and potential support for long-term implants. For this reason, it is a good support for enzyme immobilization. Enzyme immobilization could not be done directly because poly(3-hydroxybutyrate) has no functional groups. Therefore, modification should be done for enzyme immobilization. In this study, methacrylic acid was graft polymerized to poly(3-hydroxybutyrate) and thrombin was immobilized to polymethacrylic acid grafted poly(3-hydroxybutyrate). In fact, graft polymerization of methacrylic acid to poly(3-hydroxybutyrate) and thrombin immobilization was a model study. Biomolecule immobilized poly(3-hydroxybutyrate) could be used as an implant. Thrombin was selected as a biomolecule for this model study and it was immobilized to methacrylic acid grafted poly(3-hydroxybutyrate). Then the developed product was used to stop bleeding. 相似文献
8.
The enzymatic esterifications of beta-methylglucoside with acrylic acid/methacrylic acid were carried out using Novozym 435. t-Butanol indicating the highest conversion value was determined as an optimal solvent. The molar ratio (beta-methylglucoside:acids) of 1:15 was most favorable to the esterification. The enzyme concentration of 5% (w/v), and the temperature (50 degrees C for beta-methylglucoside:acrylic acid, 45 degrees C for beta-methylglucoside:methacrylic acid) resulted in the highest final conversion. Beta-methylglucoside of 60gl(-1) was found to be most effective in terms of short reaction time as well as product concentrations. Under these conditions, the maximum conversions for the esterification of beta-methylglucoside with acrylic acid and beta-methylglucoside with methacrylic acid were 59.3% after 12h and 71.3% after 72h, respectively. The structural analysis of the products was performed by FT-IR spectroscopy and (1)H NMR. 相似文献
9.
A rapid technic for the preparation of 6 μ serial sections of undecalcified bone is described. The specimen is fixed and dehydrated in acetone and ether. It is then treated with a 1:1 mixture of the monomers of ethyl and n-butyl methacrylate catalyzed with benzoyl peroxide. The monomers are removed with ether and the ether is removed under vacuum. Infiltration is accomplished under vacuum using a partial polymer of the same mixture of monomers. Polymerization is completed in 36 hours under pressure at 50°C. The tissue is sectioned with a heavy-duty microtome, the sections are mounted on glass slides and nuclear emulsions applied. Young and adult bone of laboratory animals and man have been cut successfully. Microscopic structural detail is preserved and there is no evidence of translocation of the radioactivity. 相似文献
10.
CTP : phosphocholine cytidylyltransferase activity exists in both the microsome and cytosol fractions of adult lung, 36 and 59%, respectively. Although these enzyme activities are stimulated in vitro by added lipid activators (i.e. phosphatidylglycerol), there are significant levels of activity in the absence of added lipid. We have removed endogenous lipid material from microsome and cytosol preparations of rat lung by rapid extraction with isopropyl ether. The extraction procedure did not cause any loss of cytidylyltransferase activity in the cytosol. After the extraction the enzyme was almost completely dependent upon added lipid activator. Isopropyl ether extraction of microsome preparations produced a loss of 40% of the cytidylyltransferase activity, when measured in the presence of added phosphatidylglycerol. Lipid material extracted into isopropyl ether restored the cytidylyltransferase activity in cytosol. The predominant species of enzyme activator in the isopropyl ether extracts was fatty acid. A variety of naturally occurring unsaturated fatty acids stimulated the cytidylyltransferase to the same extent as phosphatidylglycerol. Saturated fatty acids were inactive. 相似文献
11.
Löbbicke R Chanana M Schlaad H Pilz-Allen C Günter C Möhwald H Taubert A 《Biomacromolecules》2011,12(10):3753-3760
Polymer brushes on thiol-modified gold surfaces were synthesized by using terminal thiol groups for the surface-initiated free radical polymerization of methacrylic acid and dimethylaminoethyl methacrylate, respectively. Atomic force microscopy shows that the resulting poly(methacrylic acid) (PMAA) and poly(dimethylaminoethyl methacrylate) (PDMAEMA) brushes are homogeneous. Contact angle measurements show that the brushes are pH-responsive and can reversibly be protonated and deprotonated. Mineralization of the brushes with calcium phosphate at different pH yields homogeneously mineralized surfaces, and preosteoblastic cells proliferate on both the nonmineralized and mineralized surfaces. The number of living cells on the mineralized hybrid surfaces is ca. 3 times (PDMAEMA) and 10 times (PMAA) higher than on the corresponding nonmineralized brushes. 相似文献
12.
Nowadays a growing demand for green chemicals and cleantech solutions is motivating the industry to strive for biobased building
blocks. We have identified the tertiary carbon atom-containing 2-hydroxyisobutyric acid (2-HIBA) as an interesting building
block for polymer synthesis. Starting from this carboxylic acid, practically all compounds possessing the isobutane structure
are accessible by simple chemical conversions, e. g. the commodity methacrylic acid as well as isobutylene glycol and oxide.
During recent years, biotechnological routes to 2-HIBA acid have been proposed and significant progress in elucidating the
underlying biochemistry has been made. Besides biohydrolysis and biooxidation, now a bioisomerization reaction can be employed,
converting the common metabolite 3-hydroxybutyric acid to 2-HIBA by a novel cobalamin-dependent CoA-carbonyl mutase. The latter
reaction has recently been discovered in the course of elucidating the degradation pathway of the groundwater pollutant methyl
tert -butyl ether (MTBE) in the new bacterial species Aquincola tertiaricarbonis. This discovery opens the ground for developing a completely biotechnological process for producing 2-HIBA. The mutase enzyme
has to be active in a suitable biological system producing 3-hydroxybutyryl-CoA, which is the precursor of the well-known
bacterial bioplastic polyhydroxybutyrate (PHB). This connection to the PHB metabolism is a great advantage as its underlying
biochemistry and physiology is well understood and can easily be adopted towards producing 2-HIBA. This review highlights
the potential of these discoveries for a large-scale 2-HIBA biosynthesis from renewable carbon, replacing conventional chemistry
as synthesis route and petrochemicals as carbon source. 相似文献
13.
C Heiss N Hahn P Pokinskyj S Wenisch J P Stahl Ch Meyer R Schnettler 《Biomedizinische Technik》2004,49(6):163-169
In trauma surgery gluing is an attractive method of bonding fractured bone, which is rapid and does not require the use of screws and plates. The purpose of this study was to analyze in vitro the properties of a new bioresorbable bone glue, and in vivo its structure and degradation. The newly developed bone glue is based on alkylenbis(oligolactoyl)methacrylates and employs a two-component initiator system. Starting components for synthesis are ethylene glycol, lactic acid and methacrylic acid. In vitro the solidified glue is degraded via hydrolysis of ester bonds. Degradation products are ethylene glycol, lactic acid and oligomeres of methacrylic acid. After the first week polymer pellets (MMA, HEMALA, ELAMA) showed a weight loss of 12%. From week 2-20 a linear weight loss of 1.5% per week, that is 40% after 20 weeks, was observed. The in vivo investigations of the ultrastructure of the glue revealed a transparent and homogeneous mass with large electron-tight vacuoles. Differences in structure and degradation were not observed. Degradation of glue by hydrolysis and phagocytosis, with good biocompatibility was demonstrated. 相似文献
14.
Summary (1) The enzymatic removal of lipids from the vesicular membranes of the sarcoplasmic reticulum does not interfere with the fluorescence of the 1-anilino-8-naphthalenesulfonate (ANS) vesicular complex. (2) The fluorescence intensity of the ANS vesicular complex is considerably (50%) reduced by oleic acid (0.5mm) because it displaces ANS from its binding sites. (3) Stearic acid, which also combines with the membranes, interferes neither with ANS binding nor with ANS fluorescence. (4) Of all lipid compounds tested, oleylamine produces the most pronounced fluorescence enhancement of ANS. (5) The complexes formed between oleic acid and cetyltrimethyl ammonium salts or between oleic acid and polylysine produce a much higher fluorescence enhancement than the isolated components. (6) Low concentrations of ether added to ANS-containing vesicular suspensions reduce their fluorescence intensity. It returns to the initial intensity when the ether is removed. (7) A small cyclic change of the fluorescence of the vesicular ANS complex takes place during active calcium uptake. 相似文献
15.
David J. Harvey 《Molecular biotechnology》1998,10(3):251-260
The position of unsaturation, chain branching, and other structural features of fatty acids are not often apparent from the
mass spectra of common derivatives such as methyl esters because of factors such as charge location at the carboxy termiunus
and migration of double bonds. The spectra of picolinyl esters, on the other hand, contain fragment ions that provide this
information. The esters are synthesized by reaction of the acids with thionyl chloride to form the acid chloride that is reacted
with 3-pyridylcarbinol to give the ester. Under electron impact conditions in the mass spectrometer, an electron is removed
from the nitrogen of the pyridine ring and a hydrogen atom is abstracted from the alkyl chain to this electron-deficient site.
This process produces a radical site in the chain that initiates chain cleavage. Hydrogen atoms can be removed from any position
of the chain with varying probability, depending on the chain structure. Thus, diagnostic ions are produced from each type
of fatty acid whose masses and relative abundances reflect the structure of the alkyl chain and any substituents. Patterns
of fragmentation for straight-chain, branched-chain, unsaturated and cyclic fatty acids are described together with those
containing hydroxy-, epoxy-, keto-, and ether groups. 相似文献
16.
《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1995,664(2):415-420
A simple, rapid and sensitive capillary gas chromatographic method was investigated to measure portal short-chain fatty acids (SCFAs). A 200-μl sample of portal plasma was denatured with sulfosalicyclic acid and then extracted with diethyl ether before the removal of protein precipitate. The resultant extract was concentrated by a transfer to 50 μl of 0.2 M NaOH, thus avoiding tedious further concentration steps. This reduced the sample volume to one-fourth. Since the ratio of acetic acid, a major SCFA, to other acids varies widely, ranging from 10-fold to 100-fold, acrylic and methacrylic acids were used as internal standards to simultaneously measure SCFAs having a carbon number of 2–6. As a result, good recovery (90.38–103.17%) and reproducibility (coefficient of variation 0.83–8.85%) were observed over a wide range. Furthermore, portal SCFAs in rats fed various dietary fibers were determined by the present method. We showed that the amounts not only of the major acids such as acetic acid and propionic acid, but also of the minor fermented products such as n-valeric acid and n-caproic acid, could be significantly changed by dietary manipulation. Thus, the present method is simple and reliable, and requires only a small amount of sample. 相似文献
17.
Fatty acid ethyl esters, recently described as enzymatic products of nonoxidative ethanol metabolism in the heart, may represent a mediator or marker of ethanol-induced organ pathology such as alcoholic cardiomyopathy. This study was designed to develop a method for the extraction, quantitation, and definitive identification of fatty acid ethyl esters formed both in biological specimens and during enzymatic incubations. First, several potential sources of error were identified and characterized. Tissue extraction with alcohols led to the time, temperature, and concentration-dependent nonenzymatic formation of fatty acid alcohol esters. Contamination of both substrates, [14C]ethanol and 14C-fatty acid, used to measure enzymatically mediated fatty acid ethyl ester synthesis, could be removed by purification. Accurate quantitation of fatty acid ethyl esters in tissue was achieved using acetone as an extraction solvent, after which isolated lipids were thin-layer chromatographed on silica gel developed with an apolar solvent system (petroleum ether:diethyl ether:acetic acid, 75:5:1). Gas chromatography and mass spectroscopy identified individual fatty acid ethyl esters. The reproducibility of this assay was high, as assessed by quintuplicate determinations of fatty acid ethyl esters formed in liver and heart homogenates, a method with standard deviations 4 to 11% of the mean. 相似文献
18.
1. The cellular uptake and the efficiency in sensitizing cells to photoinactivation were determined for hematoporphyrin (Hp) diphenyl ether, Hp dicyclohexyl ether and Hp dihexyl ether. 2. The phenyl diether was taken up by the cells to the same degree as was the clinically used porphyrin preparation photofrin II, while the dihexyl and notably the dicyclohexyl ether were taken up 3-4 times better. 3. Furthermore, the quantum yields for photoinactivation of cells were similar for the three diethers and twice as large as that for photofrin II. 4. Fluorescence- and absorption spectroscopy indicate that these findings are related to the fact that photofrin II is much more aggregated in the cells than are the three Hp diethers. 5. When cells loaded with the porphyrins are incubated with porphyrin-free medium containing serum a certain percentage of the cell-bound drug is removed: 14% for photofrin II, 28% for Hp diphenyl ether, 50% for Hp dicyclohexyl ether and 20% for Hp dihexyl ether. 6. With respect to cell uptake and retention of the dyes, the data did not show any uniform relationship to the polarity of the drugs, in contrast to what has been found earlier for Hp diethers of linear hydrocarbons. 相似文献
19.
Güney O 《Journal of molecular recognition : JMR》2003,16(2):67-71
Lead ion templated thermosensitive heteropolymer gel which has recognition ability of methacrylate pairs has been synthesized and characterized. The gel consists of a main monomer component, N-isopropylacrylamide (NIPA), responsible for volume phase transition, methacrylic acid (MAA) moieties imprinted as pairs to adsorb terbium ions and cross-links. An imprinting technique was applied using lead ion complex with methacrylate ligands in dioxane media. After gel was obtained, lead ions were removed by washing and the imprinted gel showed strong binding ability to terbium ions, comparable with that of the non-imprinted gel prepared without lead ions. It was found that the Tb(3+) fluorescence intensity was considerably increased upon binding this ion to both imprinted and non-imprinted gels, but the largest enhancement of fluorescence intensity was observed when Tb(3+) was bound to imprinted gel in shrunken state. This is because of the decrease of coordinated water molecules on Tb(3+) and the strong binding of this ion to methacrylate pairs which are encoded within the weakly cross-linked network of imprinted gel. 相似文献
20.
Summary A determination of the ascospore producing potential forHansenula mrakii of several vegetable media, showed celery and tomato media to be much superior to all others tested, and with celery slightly better than tomato medium.The stimulatory effect on ascospore production, at least in the instance of tomato, appears to be due to a neutral substance which is not extractable with ether, is destroyed by heating under pressure in the presence of acid, and which can be removed by norite adsorption at pH 6.8 and by precipitation with silver nitrate or basic lead acetate. 相似文献