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1.
Mitsuhiro Obara Wataru Tamura Takeshi Ebitani Masahiro Yano Tadashi Sato Tomoyuki Yamaya 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(3):535-547
Root system development is an important target for improving yield in cereal crops. Active root systems that can take up nutrients
more efficiently are essential for enhancing grain yield. In this study, we attempted to identify quantitative trait loci
(QTL) involved in root system development by measuring root length of rice seedlings grown in hydroponic culture. Reliable
growth conditions for estimating the root length were first established to renew nutrient solutions daily and supply NH4
+ as a single nitrogen source. Thirty-eight chromosome segment substitution lines derived from a cross between ‘Koshihikari’,
a japonica variety, and ‘Kasalath’, an indica variety, were used to detect QTL for seminal root length of seedlings grown in 5 or 500 μM NH4
+. Eight chromosomal regions were found to be involved in root elongation. Among them, the most effective QTL was detected
on a ‘Kasalath’ segment of SL-218, which was localized to the long-arm of chromosome 6. The ‘Kasalath’ allele at this QTL,
qRL6.1, greatly promoted root elongation under all NH4
+ concentrations tested. The genetic effect of this QTL was confirmed by analysis of the near-isogenic line (NIL) qRL6.1. The seminal root length of the NIL was 13.5–21.1% longer than that of ‘Koshihikari’ under different NH4
+ concentrations. Toward our goal of applying qRL6.1 in a molecular breeding program to enhance rice yield, a candidate genomic region of qRL6.1 was delimited within a 337 kb region in the ‘Nipponbare’ genome by means of progeny testing of F2 plants/F3 lines derived from a cross between SL-218 and ‘Koshihikari’. 相似文献
2.
F. Taguchi-Shiobara S. Y. Lin K. Tanno T. Komatsuda M. Yano T. Sasaki S. Oka 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):828-833
Quantitative trait loci (QTL) controlling the regeneration ability of rice seed callus were detected using 245 RFLP markers
and 98 BC1F5 lines derived from two varieties, ‘Nipponbare’ and ‘Kasalath’. Regeneration ability was evaluated by two indices: average
number of regenerated shoots per callus (NRS) and regeneration rate (RR). The BC1F5 lines showed continuous segregation for both indices. Five putative QTL for NRS (tentatively named qRg1, qRg2, qRg4a, qRg4b and qRg4c) located on chromosomes 1, 2 and 4 were detected. Digenic interaction among these detected QTL was not significant (P<0.01). Among the five QTL detected, four ‘Kasalath’ alleles and one ‘Nipponbare’ allele increased NRS. According to an estimate
based on the nearest marker loci, the five QTL accounted for 38.5% of the total phenotypic variation of the BC1F5 lines. For RR, four putative QTL were detected on chromosomes 2 and 4, and all of these were in the same chromosomal regions
as the NRS QTL. The four RR QTL accounted for 32.6% of the total phenotypic variation.
Received: 7 November 1996 / Accepted: 25 April 1997 相似文献
3.
Akifumi Shimizu Corinta Q. Guerta Glenn B. Gregorio Shinji Kawasaki Hiroshi Ikehashi 《Plant and Soil》2005,275(1-2):57-66
Iron toxicity is one of the major constraints for lowland rice production in highly weathered soils, which are widely distributed
in tropics and subtropics and often lack macronutrients such as potassium (K) and phosphorus (P). To analyze the genetic factors
for excess iron accumulation under K or P deficiency, a set of seedlings in F3 and F8 generations from an Oryza sativa cross between a japonica cultivar ‚Gimbozu’ and an indica cultivar ‚Kasalath’ were raised and exposed to nutritional stresses in a short period under nutritional solutions. In the
F8 lines, contents of K, P, Fe, and Mg in dried shoots were measured. Quantitative trait loci (QTL) for the iron accumulation
and related mineral contents in each plant were analyzed with composite interval mapping. QTLs for the Fe, P and Mg content
in shoots were compared in the maps of F3 and F8. The QTLs for the Fe content in shoots varied in three types of nutritional conditions, but consistently indicated two overlapping
regions on chromosome 3 and 4. The obtained QTLs were crosschecked with those reported before. Some of these QTLs were indicative
of iron excluding the power of the root, which was expressed under reduced P content in solution. 相似文献
4.
Madoka Y Kashiwagi T Hirotsu N Ishimaru K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(5):603-612
Rice (Oryza sativa L.) chromosome segment substitution lines (CSSLs), in which chromosomal segments of the Indian landrace “Kasalath” replace
the corresponding endogenous segments in the genome of the Japanese premium rice “Koshihikari”, are available and together
cover the entire genome. Chromosome regions affecting a trait (CRATs) can be identified by comparison of phenotypes with genotypes of CSSLs. We detected 99 CRATs for 15 agronomic
or morphological traits. “Kasalath” had positively acting alleles in 53 CRATs. Its CRATs increased panicle number per plant
by up to 23.3%, grain number per panicle by up to 30.8%, and total grain number by up to 15.1%, relative to “Koshihikari”.
CRATs were identified for grain size (grain thickness and width), with positive effects of about 5.0%. A CRAT on chromosome 8
almost doubled the weight of roots in uppermost soil layers compared to “Koshihikari”. Additionally, “Kasalath” possessed
CRATs for higher lodging resistance (reduction in plant height and increase in stem diameter). In some cases, multiple CRATs
were detected in the same chromosome regions. Therefore, CSSLs with these chromosome segments might be useful breeding materials
for the simultaneous improvement of multiple traits. Five CRATs, one for plant height on chromosome 1, one for stem diameter
on chromosome 8, and three for heading date on chromosomes 6, 7, and 8 overlapped with the corresponding QTLs that already
had been mapped with back-crossed inbred lines of “Nipponbare” and “Kasalath”. In both “Koshihikari” CRATs and “Nipponbare”
QTLs, “Kasalath” had similar effects.
Both Y. Madoka and T. Kashiwagi have contributed equally to this article. 相似文献
5.
Comparative mapping of the wheat chromosome 5A Vrn-A1 region with rice and its relationship to QTL for flowering time 总被引:3,自引:0,他引:3
R. N. Sarma B. S. Gill T. Sasaki G. Galiba J. Sutka D. A. Laurie J. W. Snape 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):103-109
The vernalization gene Vrn-A1 on chromosome 5A is the predominant gene determining the spring/winter habit difference in bread wheat. Vrn-A1 was physically mapped using a set of deletion lines which located it to the region of chromosome 5A flanked by deletion breakpoints
0.68 and 0.78. This interval was shown to be homoeologous to a region of rice chromosome 3 that contains the flowering-time
QTL Hd-6, previously mapped in a Nipponbare×Kasalath cross, and FLTQ1, a novel QTL identified by analysis of 78 F3 families derived from a cross of ‘IR20’ב63–83’. Possible relationships between Vrn-A1 and rice QTL are discussed. Analysis of the chromosome 5A deletion lines showed evidence for a second, more proximal flowering-time
effect located between deletion breakpoints 0.56 and 0.64. The proximal part of chromosome 5A is homoeologous to rice chromosome
9, on which two QTL were detected in the ‘IR20ב63–83’ cross. The possible relationship between these effects is also discussed.
Received: 23 December 1997 / Accepted: 12 January 1998 相似文献
6.
Wenyin Zhu Jing Lin Dewei Yang Ling Zhao Yadong Zhang Zhen Zhu Tao Chen Cailin Wang 《Plant Molecular Biology Reporter》2009,27(2):126-131
Chromosome segment substitution lines (CSSLs) are powerful tools for detecting and precisely mapping quantitative trait loci
(QTLs) and evaluating gene action as a single factor. In this study, 103 CSSLs were produced using two sequenced rice cultivars:
93-11, an elite restorer indica cultivar as recipient, and Nipponbare, a japonica cultivar, as donor. Each CSSL carried a single chromosome substituted segment. The total length of the substituted segments
in the CSSLs was 2,590.6 cM, which was 1.7 times of the rice genome. To evaluate the potential application of these CSSLs
for QTL detection, phenotypic variations of seed shattering, grain length and grain width in 10 CSSLs were observed. Two QTLs
for seed shattering and three for grain length and grain width were identified and mapped on rice chromosomes. The results
demonstrate that CSSLs are excellent genetic materials for dissecting complex traits into a set of monogenic loci. These CSSLs
are of great potential value for QTL mapping and plant marker-assisted breeding (MAB). 相似文献
7.
Yusaku Uga Kazutoshi Okuno Masahiro Yano 《Molecular breeding : new strategies in plant improvement》2010,26(3):533-538
The stele (root vascular cylinder) in plants plays an important role in the transport of water and nutrients from the root
to the shoot. A quantitative trait locus (QTL) on rice chromosome 9 that controls stele transversal area (STA) was previously
detected in an F3 mapping population derived from a cross between the lowland cultivar ‘IR64’, with a small STA, and the upland cultivar ‘Kinandang
Patong’, with a large STA. To identify the gene(s) underlying this QTL, we undertook fine mapping of the locus. We screened
eight plants from BC2F3 lines in which recombination occurred near the QTL. Progeny testing of BC2F4 plants was used to determine the genotype classes for the QTL in each BC2F3 line. Accordingly, the STA QTL Sta1 (Stele Transversal Area 1) was mapped between the InDel markers ID07_12 and ID07_14. A candidate genomic region for Sta1 was defined more precisely between markers RM566 and RM24334, which delimit a 359-kb interval in the reference cultivar ‘Nipponbare’.
A line homozygous for the ‘Kinandang Patong’ allele of Sta1 had an STA approximately 28.4% larger than that of ‘IR64’. However, Sta1 did not influence maximum or total root length, suggesting that this QTL specifically controls STA. 相似文献
8.
Phosphorus deficiency-induced root elongation and its QTL in rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.) 总被引:5,自引:0,他引:5
Shimizu A Yanagihara S Kawasaki S Ikehashi H 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(7):1361-1368
A significant level of root elongation was induced in rice (Oryza sativa) grown under phosphorus-deficient conditions. The root elongation clearly varied among a total of 62 varieties screened under two different phosphorus levels. Two contrasting varieties, Gimbozu, with a low elongating response and Kasalath, with a high elongating response, were chosen and crossed to produce a hybrid population for QTL analyses. QTLs for the phosphorus deficiency-induced root elongation were detected by two linkage maps, i.e., one with 82 F3 families constructed by 97 simple sequence repeat (SSR) and sequence-tag site markers and another with 97 F8 lines by 790 amplified fragment length polymorphism and SSR markers. A single QTL for the elongation response was detected on chromosome 6, with a LOD score of 4.5 in both maps and explained about 20% of total phenotypic variance. In addition, this QTL itself, or a region tightly linked with it, partly explained an ability to reduce accumulation of excess iron in the shoots. The identified QTL will be useful to improve rice varieties against a complex nutritional disorder caused by phosphorus deficiency and iron toxicity.Electronic Supplementary Material Supplementary material is available for this article at 相似文献
9.
Taguchi K Ogata N Kubo T Kawasaki S Mikami T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(2):227-234
Aphanomyces root rot, caused by Aphanomyces cochlioides Drechs., is one of the most serious diseases of sugar beet (Beta vulgaris L.). Identification and characterization of resistance genes is a major task in sugar beet breeding. To ensure the effectiveness
of marker-assisted screening for Aphanomyces root rot resistance, genetic analysis of mature plants’ phenotypic and molecular
markers’ segregation was carried out. At a highly infested field site, some 187 F2 and 66 F3 individuals, derived from a cross between lines ‘NK-310mm-O’ (highly resistant) and ‘NK-184mm-O’ (susceptible), were tested,
over two seasons, for their level of resistance to Aphanomyces root rot. This resistance was classified into six categories
according to the extent and intensity of whole plant symptoms. Simultaneously, two selected RAPD and 159 ‘NK-310mm-O’-coupled
AFLP were used in the construction of a linkage map of 695.7 cM. Each of nine resultant linkage groups was successfully anchored
to one of nine sugar beet chromosomes by incorporating 16 STS markers. Combining data for phenotype and molecular marker segregation,
a single QTL was identified on chromosome III. This QTL explained 20% of the variance in F2 population (in the year 2002) and 65% in F3 lines (2003), indicating that this QTL plays a major role in the Aphanomyces root rot resistance. This is the first report
of the genetic mapping of resistance to Aphanomyces-caused diseases in sugar beet. 相似文献
10.
QTL analysis of Fusarium head blight resistance using a high-density linkage map in barley 总被引:2,自引:0,他引:2
Hori K Kobayashi T Sato K Takeda K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(8):1661-1672
Fusarium head blight (FHB) resistance was evaluated in a set of recombinant inbred (RI) lines from a cross between Russia
6 (resistant) and H.E.S. 4 (susceptible), which had one of the widest differences of FHB resistance reactions among ca. 5,000
barley germplasm accessions in Okayama University. Field-grown spikes were sampled and inoculated by the ‘cut-spike test’.
Resistance reactions on the parents and RI lines were scored by eleven grades, from resistant (0) to susceptible (10). Quantitative
trait loci (QTL) analysis detected three QTL: two located on the long arm of chromosome 2H, and another on the short arm of
chromosome 5H. A QTL located on chromosome 2H was coincident with the vrs1 locus, which governs inflorescence row type. The other QTL on chromosome 2H was positioned in the vicinity of cleistogamy
locus (cly1 or Cly2) that determines inflorescence opening/closing. Resistant gene analog (RGA) and expressed sequence tag (EST) markers with
homology for disease resistance genes were integrated into the high-density linkage map. Most of these markers were not localized
near the identified resistance QTL, except for one RGA marker (FXLRRfor_XLRRrev170) localized in the vicinity of the cly1/Cly2 locus. Five AFLP markers localized in the vicinity of the identified QTL were sequenced to convert them into sequence tagged
site (STS) markers. Genotyping of each RI line using two AFLP–STS markers and the vrs1 locus indicated that the RI lines with three Russia 6 QTL alleles exhibited the same level of high FHB resistance reactions
as Russia 6. In contrast, RI lines with three susceptible alleles showed reactions close to H.E.S. 4. Therefore, the markers
closely linked to the QTL can be efficiently used for the selection of resistance. 相似文献
11.
Mapping and characterization of seed dormancy QTLs using chromosome segment substitution lines in rice 总被引:1,自引:0,他引:1
Marzougui S Sugimoto K Yamanouchi U Shimono M Hoshino T Hori K Kobayashi M Ishiyama K Yano M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(5):893-902
Seed dormancy—the temporary failure of a viable seed to germinate under favorable conditions—is a complex characteristic influenced
by many genes and environmental factors. To detect the genetic factors associated with seed dormancy in rice, we conducted
a QTL analysis using chromosome segment substitution lines (CSSLs) derived from a cross between Nona Bokra (strong dormancy)
and Koshihikari (weak dormancy). Comparison of the levels of seed dormancy of the CSSLs and their recurrent parent Koshihikari
revealed that two chromosomal regions—on the short arms of chromosomes 1 and 6—were involved in the variation in seed dormancy.
Further genetic analyses using an F2 population derived from crosses between the CSSLs and Koshihikari confirmed the allelic differences and the chromosomal locations
of three putative QTLs: Sdr6 on chromosome 1 and Sdr9 and Sdr10 on chromosome 6. The Nona Bokra alleles of the three QTLs were associated with decreased germination rate. We discuss the
physiological features of the CSSLs and speculate on the possible mechanisms of dormancy in light of the newly detected QTLs. 相似文献
12.
Satoshi Ogawa Michael Gomez Selvaraj Angela Joseph Fernando Mathias Lorieux Manabu Ishitani Susan McCouch Juan David Arbelaez 《Plant and Soil》2014,375(1-2):303-315
Aims
In rice, seminal root elongation plays an important role in acquisition of nutrients such as N and P, but the extent to which different N forms and P concentrations affect root growth is poorly understood. This study aimed to examine N- and P-mediated seminal root elongation response and to identify putative QTLs associated with seminal root elongation.Methods
Seminal root elongation was evaluated in 15 diverse wild and cultivated accessions of rice, along with 48 chromosome segment substitution lines (CSSLs) derived from a cross between the rice variety ‘Curinga’ and Oryza rufipogon (IRGC 105491). Root elongation in response to different forms of N (NH4 +, NO3 ? and NH4NO3) and concentrations of P was evaluated under hydroponic conditions, and associated putative QTL regions were identified.Results
The CSSL parents had contrasting root responses to N and P. Root elongation in O. rufipogon was insensitive to N source and concentration, whereas Curinga was responsive. In contrast to N, seminal root elongation and P concentration was positively correlated. Three putative QTLs for seminal root elongation in response to N were detected on chromosome 1, and one QTL on chromosome 3 was associated with low P concentration.Conclusions
Genetic variation in seminal root elongation and plasticity of nutrient response may be appropriate targets for marker-assisted selection to improve rice nutrient acquisition efficiency. 相似文献13.
Mohamed El Soda Satya Swathi Nadakuduti Klaus Pillen Ralf Uptmoor 《Molecular breeding : new strategies in plant improvement》2010,26(4):583-593
The goal of the present study was to select BC2-lines from a cross between Hordeum vulgare and H. vulgare ssp. spontaneum and to identify introgressed candidate regions responsible for a superior pre-flowering development across environments including
drought stress conditions by using stability parameter and genotype mean estimates. Three experiments were carried out under
controlled environmental conditions. Drought stress was induced by permanent suboptimal water supply, stress cycles in continuously
drying soils, and increased transpiration demands by reducing relative humidity of the air. The environmental effects on shoot
dry weight, leaf area, tiller number, and root lengths of 36 lines and the recurrent parent, the spring barley cultivar ‘Scarlett’
was tested in ten different conditions. Results showed that 11 genotypes responded significantly (P = 0.05) different from the recurrent parent in at least one of the measured traits. The introgressions of those lines were
assigned to five genome regions, which have been suggested as QTL regions for related traits before. Regions on chromosome
4H influence tillering and one region each on 2H, 5H, and 7H probably has effects on shoot dry weight and leaf area. Introgressions
on the mentioned regions increased trait values in every case. Leaf area was highly correlated to shoot dry weight and tiller
number while the correlation between shoot dry weight and tiller number was not significant. A weak correlation was observed
between tiller number and root lengths. Slopes of response curves of lines to increasing water shortage did not significantly
differ from the population mean and from the recurrent parent. Results give hint that superior genotypes within the population
develop well under both well-watered and drought stress conditions. 相似文献
14.
Shirasawa S Endo T Nakagomi K Yamaguchi M Nishio T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(5):937-946
Low temperature at the booting stage of rice causes male sterility resulting in severe yield loss. Cold tolerance has long
been an important objective in rice breeding. We identified a quantitative trait locus (QTL) for cold tolerance on the long
arm of chromosome 3 from the cold-tolerant breeding line ‘Ukei 840’ by using F2 and BC1F2 populations from crosses between ‘Ukei 840’ and ‘Hitomebore’. The cold tolerance of ‘Ukei 840’ is derived from the Chinese
cultivar ‘Lijiangxintuanheigu’. The effect of this QTL on cold tolerance was confirmed by developing ‘Hitomebore’ chromosome
segment substitution lines having ‘Lijiangxintuanheigu’ alleles on chromosome 3. By producing recombinants in chromosome 3,
the QTL region for cold tolerance was delimited to the region of about 1.2-Mb region between RM3719 and RM7000. All lines
heterozygous for the QTL showed seed fertilities as low as that of ‘Hitomebore’, suggesting that the ‘Lijiangxintuanheigu’
allele for cold tolerance in the QTL region is recessive. Determination of a 1.2-Mb nucleotide sequence of ‘Ukei 840’ and
comparison with the published genomic sequence of ‘Nipponbare’ showed 254 SNPs, of which 11 were in coding regions of genes,
seven in five genes being non-synonymous. SNPs were detected in the 5-kb upstream regions of 89 genes, but no differences
of gene expression levels were detected between alleles of these genes. Although further delimitation is required to identify
the gene responsible for cold tolerance of ‘Lijiangxintuanheigu’, SNP markers developed here will be useful for marker-assisted
selection in a breeding program using ‘Lijiangxintuanheigu’ as a donor of cold tolerance. 相似文献
15.
U. Kumar A. K. Joshi S. Kumar R. Chand M. S. R?der 《Molecular breeding : new strategies in plant improvement》2010,26(3):477-491
Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat in warm and humid wheat growing regions of the world. To identify quantitative trait loci
(QTLs) for spot blotch resistance, two mapping populations were developed by making the crosses between common susceptible
cultivar ‘Sonalika’ with the resistant breeding lines ‘Ning 8201’ and ‘Chirya 3’. Single seed descent derived F6, F7, F8 lines of the first cross ‘Ning 8201’ × ‘Sonalika’ were evaluated for resistance to spot blotch in three blocks in each of
the 3 years. After screening of 388 pairs of simple sequence repeat primers between the two parents, 119 polymorphic markers
were used to genotype the mapping population. Four QTLs were identified on the chromosomes 2AS, 2BS, 5BL and 7DS and explained
62.9% of phenotypic variation in a simultaneous fit. The QTL on chromosome 2A was detected only in 1 year and explained 22.7%
of phenotypic variation. In the second cross (‘Chirya 3’ × ‘Sonalika’), F7 and F8 population were evaluated in three blocks in each of the 2 years. In this population, five QTLs were identified on chromosomes
2BS, 2DS, 3BS, 7BS and 7DS. The QTLs identified in the ‘Chirya 3’ × ‘Sonalika’ population explained 43.4% of phenotypic variation
in a simultaneous fit. The alleles for reduced disease severity in both the populations were derived from the respective resistant
parent. The QTLs QSb.bhu-2B and QSb.bhu-7D from both populations were placed in the same deletion bins, 2BS1-0.53-0.75 and 7DS5-0.36-0.61, respectively. The closely
linked markers Xgwm148 to the QTL on chromosome 2B and Xgwm111 to the QTL on chromosome 7D are potentially diagnostic markers for spot blotch resistance. 相似文献
16.
Mapping QTLs for pre-harvest sprouting tolerance on chromosome 2D in a synthetic hexaploid wheat×common wheat cross 总被引:1,自引:0,他引:1
Ren Xiao-bo Lan Xiu-jin Liu Deng-cai Wang Jia-li Zheng You-liang 《Journal of applied genetics》2008,49(4):333-341
Based on segregation distortion of simple sequence repeat (SSR) molecular markers, we detected a significant quantitative
trait loci (QTL) for pre-harvest sprouting (PHS) tolerance on the short arm of chromosome 2D (2DS) in the extremely susceptible
population of F2 progeny generated from the cross of PHS tolerant synthetic hexaploid wheat cultivar ‘RSP’ and PHS susceptible bread wheat
cultivar ‘88–1643’. To identify the QTL of PHS tolerance, we constructed two SSR-based genetic maps of 2DS in 2004 and 2005.
One putative QTL associated with PHS tolerance, designatedQphs.sau-2D, was identified within the marker intervalsXgwm261-Xgwm484 in 2004 and in the next year, nearly in the same position, between markerswmc112 andXgwm484. Confidence intervals based on the LOD-drop-off method ranged from 9 cM to 15.4 cM and almost completely overlapped with
marker intervalXgwm261-Xgwm484. Flanking markers near this QTL could be assigned to the C-2DS1-0.33 chromosome bin, suggesting that the gene(s) controlling
PHS tolerance is located in that chromosome region. The phenotypic variation explained by this QTL was about 25.73–27.50%.
Genotyping of 48 F6 PHS tolerant plants derived from the cross between PHS tolerant wheat cultivar ‘RSP’ and PHS susceptible bread wheat cultivar
‘MY11’ showed that the allele ofQphs.sau-2D found in the ‘RSP’ genome may prove useful for the improvement of PHS tolerance. 相似文献
17.
Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors 总被引:32,自引:0,他引:32
T. Yamamoto Y. Kuboki S. Y. Lin T. Sasaki M. Yano 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):37-44
Fine mapping was carried out on three putative QTLs (tentatively designated as Hd-1 to Hd-3) of five such QTLs controlling heading date in rice that had been earlier identified using an F2 population derived from a cross between a japonica variety, ‘Nipponbare’, and an indica variety, ‘Kasalath’, using progeny backcrossed with ‘Nipponbare’ as the recurrent parent. One BC3F2 and two BC3F1 plants, in which the target QTL regions were heterozygous and most other chromosomal regions were homozygous for the ‘Nipponbare’
allele, were selected as the experimental material. Self-pollinated progeny (BC3F2 and BC3F3) of the BC3F1 or BC3F2 showed continuous variation in days to heading. By means of progeny testing based on BC3F3 or BC3F4 lines, we determined the genotypes of each BC3F2 or BC3F3 individual at target QTLs. Their segregation patterns fitted Mendelian inheritance ratios. When the results obtained by RFLP
analysis and progeny tests were combined, Hd-1, Hd-2 and Hd-3 were mapped precisely on chromosomes 6, 7 and 6, respectively, of a rice RFLP linkage map. The results demonstrated that
QTLs can be treated as Mendelian factors. Moreover, these precise locations were in good agreement with the regions estimated
by QTL analysis of the initial F2 population, demonstrating the high reliability of QTL mapping using a high-density linkage map.
Received: 5 November 1997 / Accepted: 10 February 1998 相似文献
18.
Chromosome segment substitution lines (CSSLs) are useful for the precise mapping of quartitative trait loci (QTLs) and dissection of the genetic basis of complex traits.In this study,two whole-genome sequenced rice cultivars,the japonica Nipponbare and indica 9311 were used as recipient and dtonor,respectively.A population with 57 CSSLs was developed after crossing and back-crossing assisted by mo lecular rnarkers,and genotypes were identified using a high-throughput resequencing strategy,Detailed graphical genotypes of 38 lines were constructed based on resequencing data.These CSSLs had a total of 95 substituted segments derived from indica 9311,with an average of about 2.5 segments pet CSSL and eight segments per chromosome,and covered about 87.4% of the rice whole genome.A multiple linear regression QTL analysis mapped four QTLs for 1000-grain weight.The largest-effect QTL was located in a region on chromosome 5 that contained a cloned major QTL GW5/qSW5 for grain size in rice.These CSSLs with a background of Nipponbare may provide powerful tools for future whole-genome discovery and functional study of essential genes/QTLs in rice,and offer ideal materials and foundations for japonica breeding. 相似文献
19.
R. Mizobuchi H. Sato S. Fukuoka S. Tsushima T. Imbe M. Yano 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(9):2417-2425
Bacterial seedling rot (BSR), a destructive disease of rice (Oryza sativa L.), is caused by the bacterial pathogen Burkholderia glumae. To identify QTLs for resistance to BSR, we conducted a QTL analysis using chromosome segment substitution lines (CSSLs) derived from a cross between Nona Bokra (resistant) and Koshihikari (susceptible). Comparison of the levels of BSR in the CSSLs and their recurrent parent, Koshihikari, revealed that a region on chromosome 10 was associated with resistance. Further genetic analyses using an F5 population derived from a cross between a resistant CSSL and Koshihikari confirmed that a QTL for BSR resistance was located on the short arm of chromosome 10. The Nona Bokra allele was associated with resistance to BSR. Substitution mapping in the Koshihikari genetic background demonstrated that the QTL, here designated as qRBS1 (quantitative trait locus for RESISTANCE TO BACTERIAL SEEDLING ROT 1), was located in a 393-kb interval (based on the Nipponbare reference genome sequence) defined by simple sequence repeat markers RM24930 and RM24944. 相似文献
20.
Matsubara K Ando T Mizubayashi T Ito S Yano M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(2):179-186
One outcome of hybrid breakdown is poor growth, which we observed as a reduction in the number of panicles per plant and in
culm length in an F2 population derived from a cross between the genetically divergent rice (Oryza sativa L.) cultivars ‘Sasanishiki’ (japonica) and ‘Habataki’ (indica). Quantitative trait locus (QTL) analysis of the two traits and two-way ANOVA of the detected QTLs suggested that the poor
growth was due mainly to an epistatic interaction between genes at QTLs located on chromosomes 2 and 11. The poor growth was
likely to result when a plant was homozygous for the ‘Habataki’ allele at the QTL on chromosome 2 and homozygous for the ‘Sasanishiki’
allele at the QTL on chromosome 11. The results suggest that the poor growth found in the F2 population was due to hybrid breakdown of a set of complementary genes. To test this hypothesis and determine the precise
chromosomal location of the genes causing the hybrid breakdown, we performed genetic analyses using a chromosome segment substitution
line, in which a part of chromosome 2 from ‘Habataki’ was substituted into the genetic background of ‘Sasanishiki’. The segregation
patterns of poor growth in plants suggested that both of the genes underlying the hybrid breakdown were recessive. The gene
on chromosome 2, designated hybrid breakdown 2 (hbd2), was mapped between simple sequence repeat markers RM3515 and RM3730. The gene on chromosome 11, hbd3, was mapped between RM5824 and RM1341.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献