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Baron O Förthmann B Lee YW Terranova C Ratzka A Stachowiak EK Grothe C Claus P Stachowiak MK 《The Journal of biological chemistry》2012,287(24):19827-19840
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Nonconservative utilization of aldolase A alternative promoters 总被引:5,自引:0,他引:5
J K Stauffer M C Colbert E Ciejek-Baez 《The Journal of biological chemistry》1990,265(20):11773-11782
Recently, analysis of the sequence and expression of the human aldolase A gene revealed the unique arrangement of three tandem promoters and exons preceding a common coding sequence. A muscle-specific promoter (M) and two flanking widely used promoters (N and H) produce mRNA species which, in their mature forms, differ only in the sequence of their 5'-untranslated regions. We have isolated and investigated the expression of a mouse aldolase A gene. This mouse gene represents a functional gene by sequence analysis, recombinational screening, and by transfection into C2C12 cells. Although there is a high degree of sequence similarity between the mouse and the human gene in the region of the alternative first exons, we have been unable to detect a functional utilization of the 5'-most promoter (N) in the mouse. Steady state mRNAs isolated from a variety of adult tissues and cultured cells were analyzed by RNase protection and primer extension to identify first exon utilization. Consistent with previous reports, exon M is found only in skeletal muscle and exon H, the "housekeeping" exon, is utilized in every tissue where aldolase A is expressed. Under identical conditions we fail to see any evidence of the N exon. Therefore, although sequence homology exists between rodents and primates in the N region, the absence of selective pressure to preserve its primate pattern of expression may have resulted in functional promoter extinction. 相似文献
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Organization of the human orphan nuclear receptor Nurr1 gene 总被引:5,自引:0,他引:5
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