LignicolousHyphomycetes from Czechoslovakia 1.Brachysporium

Four species of the genusBrachysporium Sacc. emend.Mason etHughes are treated in this paper from Czechoslovakia:B. nigrum (Link)Hughes,B. obovatum (Berk.)Sacc. and two new species,B. abietinum Hol.-Jech. andB. brevius Hol.-Jech.     

A revision ofTaraxacum sect.Piesis (Compositae)

On the basis of allozyme and cultivation data, and of additional herbarium material, a taxonomic and nomenclatural revision ofTaraxacum sect.Piesis A.J. Richards exKirschner et?těpánek is provided. The section is made up of halophilous, sexually reproducing taxa. InT. stenocephalum Boiss. etKotschy,T. pindicum Kirschner et?těpánek, sp. nov., andT. perenne Kirschner et?těpánek, sp. nov., a tetraploid chromosome number has been recorded, representing the only known case of sexuality at the tetraploid level in the genus. The complex ofT. stenocephalum, includes some geographically and morphologically extreme populations treated as subspecies: subsp.gumusanicum (Soest)Kirschner et?těpánek, comb. nov., subsp.magnum Kirschner et?těpánek, subsp. nov., and subsp.daralagesicum (Schischk.)Kirschner et?těpánek, comb. nov. In addition toT. bessarabicum (Hornem.)Hand.-Mazz., a widely distributed Eurasian species,T. stenocephalum, a complex centred in Transcaucasia and Anatolia, andT. pachypodum H. Lindb., a North African endemic, four new species are described:T. salsum Kirschner et?těpánek, sp. nov., a diploid endemic confined to E Crimea,T. perenne Kirschner et?těpánek, sp. nov., a tetraploid sexual species known only from SW Crimea,T. pindicum Kirschner et?těpánek, sp. nov., a remarkable tetraploid endemic to the Pindos Mts., Greece, andT. salsitatis Kirschner, ?těpänek etYirdirimli, sp. nov., an Anatolian diploid species. Furthermore, a hybrid betweenT. salsum andT. bessarabicum from Crimea (documented on the basis of allozyme data elsewhere) is given a binomial,T. xmesohalobium Kirschner et?těpánek, nothosp. nov.     

LignicolousHyphomycetes from Czechoslovakia 3.Sporoschisma,Sporoschismopsis andCatenularia

The species of three genera—Sporoschisma Berk. etBr.,Sporoschismopsis Hol.-Jech. etHennebert andCatenularia Grove are treated in this paper from Czechoslovakia:Sporoschisma juvenile Boudier,S. mirabile Berk. etBr.,Sporoschismopsis moravica Hol.-Jech. etHennebert andCatenularia cuneiformis (Richon) Mason. The studied fungi produce the conidia in basipetal succession, endogenously or subendogenously, quite internally in large phialides or only inside open swollen collarettes.     

LignicolousHyphomycetes from Czechoslovakia 2.Bactrodesmium

Six species of the genusBactrodesmium M. C. Cooke are described in this paper from Czechoslovakia:B. betulicola M.B. Ellis,B. obovatum (Oudemans) M.B. Ellis,B. spilomeum (Berk. etBr.)Mason etHughes including three new speciesB. globosum Hol.-Jech.,B. pyriforme Hol.-Jech. andB. submoniliforme Hol.-Jech.     

Notes on some African hepatic genera 1–5

The present study deals with five genera of hepatics in Africa, Isotachis Mitt., Anastrophyllum (Spruce) Steph., Tritomaria Schiffn. ex Loeske, Gymnocoleopsis (Schust.) Schust. and Lophozia (Dum.) Dum. All African populations of the genus Isotachis Mitt. are considered to be one species, I. aubertii (Schwaegr.) Mitt. Four species of Anastrophyllum (Spruce) Steph. (s.l.), A. auritum (Lehm.) Steph., A. piligerum (Nees) Spruce, A. subcomplicatum (Lehm. et Lindenb.) Steph. and A. minutum (Schreb.) Schust., and two species of Tritomaria Schiffn. et Loeske, T. camerunensis S. Arnell and T. exsecta (Schrad.) Schiffn. ex Loeske occur in Africa. Gymmocoleopsis multiflora (Steph.) Schust. represents a genus and species hitherto unreported for the African flora. Finally, five Lophozia (Dum.) Dum. species, L. argentina (Steph.) Schust., L. capensis S. Arnell, L. decolorans (Limpr.) Steph., L. hedbergii S. Arnell and L. tristaniana (S. Arnell) Váňa, are reported from central and southern Africa; two of these (L. argentina (Steph.) Schust. and L. decolorans (Limpr.) Steph.) represent the first reports from Africa.     

Contribution to the knowledge ofEchinodorus macrophyllus (Kunth)Mich. andE. scaber Rataj from tropical America

The paper deals with the taxonomy and geographical distribution of the two species of the genusEchinodorus whichFassett (1955) did not work up satisfactorily in his revision i.e.Echinodorus macrophyllus (Kunth)Mich. whichFassett does not quote at all, andE. scaber Rataj whichFassett mistook forE. muricatus Griseb. Further a new variety and the diagnoses of hybrids, among whichE. punctatus Mich. is included, are cited.     

Ein Beitrag zur Nomenklatur sphinctozoider Schwämme (Porifera)

The sphinctozoid sponge generaFania Senowbari-Daryan 1990 andSpica Termier &Termier 1977 are preoccupied.Fania is replaced byFanthalamia nom. nov. andSpica by the younger synonymFistulispongia Termier &Termier 1977. The invalid subfamily name FaniinaeSenowbari-Daryan 1990 is replaced by Fanthalamiinae n. subfam. The invalid family and subfamily names SpicidaeTermier &Termier 1977 and SpicinaeSenowbari-Daryan 1990 respectively are replaced by FistulispongiidaeTermier atTermier 1977 and FistulispongiinaeSenowbari-Daryan 1990. The generaWaagenium de Laubenfels 1957 andCatubria Merla 1931 were previously overlooked.Waagenium DeLaubenfels 1957 is a younger synonym ofColospongia Laube 1865. The position ofCatubria Merla 1931 is uncertain. Most probablyCatubria is an alga.     

LignicolousHyphomycetes from Czechoslovakia 5.Septonema,Hormiactella andLylea

Three genera of lignicolousHyphomycetes Septonema Corda,Hormiactella Saac. andLylea Morgan-Jones are discussed. Illustrations and determination keys are provided. Five species ofSeptonema—S. fasciculare (Corda) Hughes,S. leptaleum (Ellis & Harkn.) Hughes,S. pinicola Hol.-Jech.,S. secedens Corda andS. laricium Hol.-Jech.—and two species ofHormiactella—H. fusca (Preuss) Sacc. andH. asetosa Hol.-Jech.—have been found in Czechoslovakia. A new speciesS. pseudobinum Hol.-Jech. is described from Romania.Septonema tetracoilum (Corda Hughes is accommodated in the genusLylea Morgan-Jones.     

LignicolousHyphomycetes from Czechoslovakia 6.Spadicoides andDiplococcium

Seventeen species belonging toSpadicoides Hughes andDiplococcium Grove collected in Central Europe mostly in Czechoslovakia are described and illustrated. Two new species are described inSpadicoides—S. carpatica Hol.-Jech.,S. penatium Hol.-Jech. and three new species inDiplococcium—D. bicolor Hol.-Jech.,D. insolitum Hol.-Jech. andD. parcum,Hol.-Jech.     

The variability of critical species ofLotus L. in Iran and in the neighbouring countries II

The paper deals with some problems of distribution and variability of the speciesL. angustissimus L.,L. halophilus Boiss. etSprun.,L. laricus Rech. fil.,Aellen etEsfandiari, L.,schimperi Steud.,L. compactus Chrtková-?ertová,L. garcinii DC.     

Hybridization studies inSilene subgen.Petrocoptis (Caryophyllaceae)

Silene subgenusPetrocoptis comprises sexual diploid taxa and it is restricted to calcareous cliffs in the Iberian peninsula. Artificial crosses involvingSilene pyrenaica (Bergeret)Mayol etRosselló (≡Petrocoptis pyrenaica (Bergeret)Walp.),Silene laxipruinosa Mayol etRosselló andS. montserratii subsp.crassifolia (Rouy)Mayol etRosselló (≡P. crassifolia Rouy) were attempted to assess the extent of barriers developed within the subgenusPetrocoptis. Usually, intraspecific crosses involving allopatric populations were successful, suggesting that geographically isolated populations are not genetically isolated. Cross-compatibility was noted among the polymorphicS. pyrenaica, which developed fertile F₁ hybrids. All other interspecific crosses failed due to cross- or seed-incompatibility. Crossing results agree with available evidence supporting the merging of segregates ofS. pyrenaica within a single taxon.     

On the identity and systematic position of the genusDirphys [Hym.: Aphelinidae]

Types have been examined of the aphelinid genusDirphys Howard (Type species,Mesidia mexicana Howard). It is found to be closely related toEncarsia Förster and related coccophagine genera. Hence its earlier placement in the subfamilyAphelininae sensu De Santis orAphytinae Jasnosh is considered untenable and, consequently, it is transferred to subfamilyCoccophaginae sensu De Santis.     

Characterization of a recombinant l-rhamnose isomerase from Bacillus subtilis and its application on production of l-lyxose and l-mannose

The gene of an l-rhamnose isomerase (RhaA) from Bacillus subtilis was cloned to the pET28a(+) and then expressed in the E. coli ER2566. The expressed enzyme was purified with a specific activity of 3.58 U/mg by His-Trap affinity chromatography. The recombinant enzyme existed as a 194 kDa tetramer and the maximal activity was observed at pH 8.0 and 60°C. The RhaA displayed activity for l-rhamnose, l-lyxose, l-mannose, d-allose, d-gulose, d-ribose, and l-talose, among all aldopentoses and aldohexoses and it showed enzyme activity for l-form monosaccharides such as l-rhamnose, l-lyxose, l-mannose, and l-talose. The catalytic efficiency (k _cat/K _m) of the recombinant enzyme for l-rhamnose, l-lyxose, and l-mannose were 7,460, 1,013, and 258 M/sec. When l-xylulose 100 g/L and l-fructose 100 g/L were used as substrates, the optimum concentrations of RpiB were determined with 6 and 15 U/mL, respectively. The l-lyxose 40 g/L was produced from l-xylulose 100 g/L by the enzyme during 60 min, while l-mannose 25 g/L was produced from l-fructose 100 g/L for 80 min. The results suggest that RhaA from B. subtilis is a potential producer of l-form monosaccharides.     

LignicolousHyphomycetes from Czechoslovakia 8.Endophragmiella andPhragmocephala

Thirteen species and one variety ofEndophragmiella Sutton and three species ofPhragmocephala Mason etHughes collected by the author in Czechoslovakia, and occasionally in other regions of Europe are described and illustrated. Four new species and one new variety are described inEndophragmiella—E. aseptata Hol.-Jech.,E. fatrensis Holt.-Jech.,E. ramificata Hol.-Jech.,E. similis Hol.-Jech. andE. uniseptata (Ellis) Hughes var.pusilla Hol.-Jech..     

Notes on the genusOenothera subsect.Oenothera (Onagraceae) in the Czech Republic

Up-to-date results of taxonomic and phytogeographic studies ofOenothera subsect.Oenothera in the eastern part of the Czech Republic (Moravia and Silesia) are presented. A new hybridogenous species,Oe. moravica Jehlík etRostański (=Oe. fallax × Oe. victorini), is described from S Moravia. Several taxa and hybrids are reported for the first time for the Czech Republic:Oe. victorini Gates etCatcheside (=Oe. rostanskii Jehlík),Oe. × wienii Renner exRostański,Oe. acutifolia × Oe. victorini. Other taxa are recorded from the eastern part of the Czech Republic for the first time:Oe. acutifolia Rostański,Oe. hoelscheri Renner exRostański,Oe. parviflora L.,Oe. subterminalis Gates. The taxonomy ofOe. pycnocarpa Atkinson etBartlett is discussed. A survey of species and hybrids ofOenothera subsect.Oenothera in the Czech Republic is added.