Development of microsatellite markers for Carapa guianensis (Aublet), a tree species from the Amazon forest

Carapa guianensis is a timber species found in Central America and the north of South America. We have developed microsatellite primers which will allow analysis of gene flow and population genetic structure in natural populations of this tree species. Polymorphism of five microsatellite loci was evaluated using a total of 12 adult trees from a natural population. An average of 4.2 alleles per locus was detected, and expected heterozygosity ranging from 0.397 to 0.806. These loci are being used for genetic population analysis in a managed forest in the state of Pará, in Amazonian Brazil, as part of the Dendrogene project.     

Lábrea hepatitis in Salvador, Bahia? (Presentation of a possible case)

A case of a four-year-old boy from Salvador, Bahia, Brazil, with a probable Lábrea hepatitis is reported. He ran a rapid course of fever, jaundice, abdominal pain, agitation, coma and death. Histologically, the liver presented widespread hepatic cell lytic and coagulative necrosis, acute fatty changes, mononuclear cell infiltration and cholestasis. The study of the case raises the question whether Lábrea hepatitis can occur outside the Amazonian region, or else its clinico-pathological features lack specificity.     

Protura in native and exotic forests in the North Island of New Zealand

Abstract

This study provides data on population and community ecology of Protura in native forests and Pinus radiata plantations in New Zealand. Abundance, age structure, sex ratios, biodiversity, and relationship with soil chemistry are discussed. Protura were significantly more abundant in pine plantations in comparison to native forests. Among native forests, Protura were most abundant under Southern beech (Nothofagus solandri). The abundance patterns may reflect the association between Protura and fungal communities in the soil. No correlation was found between soil organic matter, nitrogen, phosphorous, pH, cation exchange capacity, and the density of Protura. Protura assemblage composition was significantly related to forest type. New Zealand endemic species were associated with native forests; species with distribution outside New Zealand dominated in pine plantations. The distribution records within New Zealand were expanded for five species. The Protura fauna of New Zealand was increased to 18 species. Berberentulus capensis, Eosentomon australicum and Australentulus tillyardi are new records for New Zealand fauna.     

Geographical zonation in the Neotropics of tree species characteristic of the Paraguay-Paraná Basin

Aim This paper uses data from the literature (monographs and taxonomic reviews) to investigate the geographical zonation in South America of 32 common tree species encountered in Paraguay. The actual spatial distribution of the species is then used to provide clues on the plant communities present in the past, and especially during the Wisconsinan age (Last major ice age: 80,000–10,000 bp ). The floristic relatedness between the Paraguayan flora and the neighbouring floras is also investigated. Methods The main vegetation patterns were highlighted using a Discriminant Analysis of the Eigenvectors of Neighbourhood Operator to emphasize the geographical zonation in South America of 32 tree species predominant in the Paraguay‐Paraná Basin. Results Three main vegetation patterns were emphasized: the Chaco, the Paraná‐São Francisco gradient and the Peri‐Amazonian gradient. The Chaco is well defined. The Peri‐Amazonian gradient is characterized by a continuous change in species from the Colombian pole towards the São Francisco pole. The São Francisco gradient shows a continuous change from the São Francisco pole towards the Paraná pole. Some of the species are monocentric (related to only one dispersal centre), whereas others are polycentric (distributed in several poles). Main conclusions The Chaquean xeromorphic forests are considered as a climax, with a stable composition resulting of the saline soil conditions. The Chaco acts as an edaphic barrier to many species, although the migration of some non‐Chaquean species is made possible by the net of gallery‐forests and low montane forests between the Río Paraguay and the Andean Piedmont. The Cerrado and the Chaco can be considered as a barrier limiting the expansion of the Paranean forest. This latter formation is a semi‐deciduous forest made of a mix of monocentric and polycentric elements. The numerous species found both in Colombia and in Paraná indicate that bridges exist, or have existed, between these two poles. The persistence in the Paraguay‐Paraná Basin of a moist forest made of the Paranean assemblages highlighted in this study confirms the hypothesis of moister and cooler phases than today.     

Observations on the vegetation of northeastern Mato Grosso II. Forests and soils of the Rio Suiá--Missu area.

The vegetation of the well drained soils along the Suiá--Missu road in the Serra do Roncador region of NE Mato Grosso is Evergreen Seasonal forest of Amazonian type. The area lies close to the meeting place of the Amazonian forest (the hylaea) and the cerrado (savanna) formation of Central Brazil. The structure of the forest is simple: the canopy is at about 18--23 m, and is exceeded by a few scattered emergents; no recognizable strata can be distinguished among the understorey trees and the shrub and herb layers are sparse. Table 1 lists the most important species and gives information on stratification and general distribution. Most of the species appear to have a hylaean centre of distribution but extend into other vegetation types. The forest differs from related communities which lie closer to the cerrado/forest boundary in its greater height and luxuriance, the presence of additional tall tree species, and the great reduction in abundance of a cerrado floristic element. A survey on the Xavantina--S?o Felix road allowed us to extend previous observations on the distance to which the cerrado tree Pterodon pubescens extends into the forest. The results obtained indicate a considerable extension of forest into cerrado during the life of an individual tree. A characteristic low forest occurs in the flood plain of the Rio Suiá--Missu while Swampy Gallery forests occur on permanently waterlogged soils around the headwaters of streams. The well drained soils of the Suiá--Missu forest are very uniform, deep latosols (oxisols) of very dystrophic nature with pH (in water) between 4.0 and 5.0 (see table 2, p. 203).     

Length–weight relationship of 10 freshwater fish species in Amazonian streams,Trombetas River basin (Brazil)

Length–weight relationships (LWR) were analyzed for 10 fish species from 26 Amazonian streams located in four micro‐basins in the Trombetas River basin, Pará State, Brazil. LWRs were calculated based on 1831 individuals sampled between 2010 and 2012 using a manual trawl. The coefficients of proportionality and allometry were within the ranges estimated for most fishes. No previous LWR information was available for these species in the literature.     

Finding needles in the haystack: where to look for rare species in the American tropics

Tropical America (the Neotropics) harbours more plant species than any other region on Earth. The contribution of rare species to this diversity has been recently recognised, but their spatial distribution remains poorly understood. Here, we use all collection records of angiosperms from the Global Biodiversity Information Facility to delineate Neotropical bioregions, and to identify putatively rare species within the Neotropics and the Amazonian rainforest. We analyse the spatial distribution of these species and validate the results on a largely independent dataset based on vegetation plots from the Amazon Tree Diversity Network. We find that rare species are homogeneously distributed through most parts of the lowland Neotropics and Amazonia, but more concentrated in highlands. The second collection of any rare species is most often found in the close vicinity of the first, but in 20% of cases they are more than 580 km apart. We also find cross‐taxonomic patterns of disjunct distributions within the Andes, the Atlantic forest in eastern Brazil, and between Amazonia and the Atlantic forest, but no clear disjunction patterns within lowland areas. These results suggest that a considerable proportion of rare plant species have surprisingly large distribution ranges, and that collections of rare species across most of the lowland Neotropics, and in particular in Amazonia, show no clear directionality. The second record of many rare species may be found virtually anywhere, urging the need for intensifying and broadening biological sampling.     

Leishmania (Viannia) utingensis n. sp., a parasite from the sandfly Lutzomyia (Viannamyia) tuberculata in Amazonian Brazil

A leishmanial parasite isolated in 1977 from a specimen of the sandfly Lutzomyia tuberculata from Pará State, Amazonian Brazil, has been characterized following its comparison with other species of Leishmania from the same region, using isoenzyme profiles, monoclonal antibodies and characterization of the miniexon gene repeat, using the polymerase chain reaction technique (PCR). It is described here under the name of Leishmania (Viannia) utingensis n. sp.     

Areas of endemism and distribution patterns for Neotropical Piper species (Piperaceae)

Aim The study aimed to establish areas of endemism and distribution patterns for Neotropical species of the genus Piper in the Neotropical and Andean regions by means of parsimony analysis of endemicity (PAE) and track‐compatibility analysis. Location The study area includes the Neotropical region and the Northern Andean region (Páramo‐Punan subregion). Methods We used distribution information from herbarium specimens and recent monographic revisions for 1152 species of Piper from the Neotropics. First, a PAE was attempted in order to delimit the areas of endemism. Second, we performed a track‐compatibility analysis to establish distribution patterns for Neotropical species of Piper. Terminology for grouping Piper is based on recent phylogenetic analyses. Results The PAE yielded 104 small endemic areas for the genus Piper, 80 of which are in the Caribbean, Amazonian and Paranensis subregions of the Neotropical region, and 24 in the Páramo‐Punan subregion of the Andean region. Track‐compatibility analysis revealed 26 generalized tracks, one in the Páramo‐Punan subregion (Andean region), 19 in the Neotropical region, and six connecting the Andean and Neotropical regions. Both the generalized tracks and endemic areas indicate that distribution of Piper species is restricted to forest areas in the Andes, Amazonia, Chocó, Central America, the Guayana Shield and the Brazilian Atlantic coast. Main conclusions Piper should not be considered an Andean‐centred group as it represents two large species components with distributions centred in the Amazonian and Andean regions. Furthermore, areas of greater species richness and/or endemism are restricted to lowland habitats belonging to the Neotropical region. The distribution patterns of Neotropical species of Piper could be explained by recent events in the Neotropical region, as is the case for the track connecting Chocó and Central America, where most of the species rich groups of the genus are found. Two kinds of event could explain the biogeography of a large part of the Piper taxa with Andean–Amazonian distribution: pre‐Andean and post‐Andean events.     

A new species of Amblyodus Westwood, 1878 (Coleoptera, Melolonthidae, Dynastinae) from South America

A second species of Amblyodus Westwood, 1878, Amblyodus castroi sp. n., is described from the northern South America based on 12 specimens from Brazil and Peru (Amazonian subregion). The new species is here compared with the type species of the genus, Amblyodus taurus Westwood, 1878 and both species and their male genitalia are illustrated. Diagnostic characters for the genus are discussed, especially the metatibial teeth. A distribution map including the type species and the new species is provided. The genus Amblyodus is recorded for the first time from Peru and from Brazil states of Pará and Rondônia.     

Thelypteris amazonica sp. nov. (Thelypteridaceae) from Amazonian Brazil

A new species of Thelypteris subgenus Goniopteris (Thelypteridaceae) is described and illustrated: Thelypteris amazonica Salino & R. S. Fernandes is apparently most similar to Thelypteris anoptera (Kunze) C. Chr. (Brazil‐Bahia) and T. abrupta (Desv.) Proctor (Antilles, Central America, South America), but differs from both by the clavate vein ends, basal vein pairs from adjacent segments not united below the sinuses, and petioles with simple and stellate hairs. Thelypteris amazonica is known only from Amazonian Brazil.     

卷叶薄齿藓和北美赤藓形态特征及其地理新分布

通过对采自贵州省梵净山和内蒙古赛罕乌拉自然保护区的苔藓植物标本进行鉴定,发现了丛藓科的2个新分布种——卷叶薄齿藓(Leptodontium pungens)和北美赤藓(Syntrichia amphidiacea)。卷叶薄齿藓为中国新记录种,首次在中国贵州省梵净山发现,其主要特征为茎无中轴分化,具透明层,常分布于火山和温带高海拔地区。北美赤藓为内蒙古新分布种,发现于内蒙古赛罕乌拉自然保护区,其主要特征为叶腹面近叶尖处生有大量芽胞,成熟芽胞棕色,多细胞,圆柱状。本研究对卷叶薄齿藓和北美赤藓的形态特征和地理分布进行了具体描述,并提供了图版和分种检索表。     

Distribution and population structure of four Central Amazonian high-várzea timber species

Amazonian white-water (várzea) floodplains harbor many commercially important timber species which in Brazil are harvested following regulations of the Federal Environmental Agency (IBAMA). Although it is well-known that tree physiology, growth, and species distribution of Amazonian floodplain trees is linked to the heights and durations of the periodical inundations, information about timber stocks and population dynamics is lacking for most tree species. We investigated timber stocks and the population structure of four intensely logged tree species in a western Brazilian várzea forest on an area totaling 7.5 ha. Spatial distribution was investigated in all trees as a function of inundation height and duration and the distance to the river channel, and additionally for saplings (trees <10 cm diameter at breast height––DBH) as a function of the relative photosynthetically active radiation (rPAR). The diameter-class distribution in Hura crepitans and Ocotea cymbarum indicated that populations are subject to density variations that possibly are traced to small-scale flood variability. In all species, saplings concentrated at higher topographic elevations than the mature tree populations, which suggest that the physical ‘escape’ from a flooded environment is an important acclimation to flooding. While Ocotea cymbarum and Guarea guidonia were high-density wood species characterized by random dispersion and a pronounced shade-tolerance, Hura crepitans and Sterculia apetala presented lower wood density, aggregated dispersion, and were more light-demanding. All species presented exploitable stems according to the current harvest regulations, with elevated abundances in comparison to other Amazonian forest types. However, stem densities are below the harvest rates indicating that the harvest regulations are not sustainable. We recommend that the forest management in várzea forests should include specific establishment rates of timber species in dependence of the peculiar site conditions to achieve sustainability.     

Habitat specifity,endemism and the neotropical distribution of Amazonian white‐water floodplain trees

.The Amazon basin is covered by the most species‐rich forests in the world and is considered to house many endemic tree species. Yet, most Amazonian ecosystems lack reliable estimates of their degree of endemism, and causes of tree diversity and endemism are intense matters of debate. We reviewed the spatial distribution of 658 of the most important flood‐tolerant Amazonian white‐water (várzea) tree species across the entire Neotropics by using data from herbaria, floras, inventories and checklists. Our results show that 90% of the várzea tree species are partially or widely distributed across neotropical macro‐regions and biomes. Chi‐square analyses indicated that várzea species richness in non‐várzea macro‐regions was dependent on the flooding gradient and the longitudinal position. Cluster analysis combined with association tests indicated four significant patterns of várzea species distributions depending on species flood‐tolerance (low vs high) and spatial distribution (restricted vs widespread). We predict that the predominance of Andean substrates is the most important factor that determines the distribution of várzea tree species within and beyond the Amazon basin and explains the high floristic similarity to the Orinoco floodplains. Distribution patterns in other extra‐Amazonian macro‐regions are more likely linked to climatic factors, with rainforest climates housing more várzea species than savanna climates. 130 tree species were restricted to South‐American freshwater floodplains, and 68 (> 10%) were endemic to Amazonian várzea. We detected two centers of endemism, one in the western Amazon characterized by low and brief floods, and one in the central Amazon, characterized by high and prolonged floods. Differences in taxonomic composition of endemic centers in the western and central Amazon are the result of different abiotic factors (i.e. flood regimes), as well as the regional species pools from where the species are recruited from. We hypothesize that numerous morphological, physiological and biochemical adaptations permit survival of trees in flooded environments. Furthermore, these adaptations are independently derived across many taxa and result in a highly specialized flora. We attribute higher than expected levels of endemism to the great spatial extent and age of floodplain ecosystems in the Amazon basin, and highlight the role of Amazonian várzea as an potential driver in speciation and diversification processes.     

Revision of Ticorea Aubl. (Rutaceae, Galipeinae)

Ticorea comprises five species, which occur in the Guianas, throughout the Amazonian basin, and on the lower eastern slopes of the Andes in Ecuador, Peru, and Bolivia. Two of the five are described here as new: T. diandra, from eastern Ecuador and adjacent Peru, and T. froesii, from Maranhão and Pará, Brazil.     

Analysis of the sex pheromone extract of individual male Lutzomyia longipalpis sandflies from six regions in Brazil

Although the phlebotomine sandfly Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) is generally accepted to be a species complex, it is unclear how many members there are, how they are related and which are the main vectors of leishmaniasis. The vectorial capacity of each sibling species is likely to differ, thus a means of identifying the most important vector species is of critical importance to the epidemiology and control of this debilitating disease in South and Central America. In Brazil four chemotypes have been distinguished by sex pheromone analysis. In this study the sex pheromone extracts of L. longipalpis from six regions of Brazil were analysed in detail. Samples included the sympatric 1-spot, 2-spot and intermediate spot morphotypes from Sobral, Ceará State. The results strongly suggest that members of the complex that produce different sex pheromones are reproductively isolated, thus strengthening the argument that the different chemotypes represent true sibling species. The study also found significant differences in morphology and the amounts of sex pheromone produced by members of each chemotype from different parts of Brazil, which suggests population substructuring that has not previously been recognized. Evidence of a fifth chemotype in Brazil is also presented.     

Isolation and identification of 9-methylgermacrene-B as the putative sex pheromone of Lutzomyia cruzi (Mangabeira, 1938) (Diptera: Psychodidae)

Lutzomyia (Lutzomyia) cruzi has been named as a probable vector of Leishmania chagasi in Corumbá, Mato Grosso do Sul, Brazil. Taxonomically L. cruzi is closely related to the L. longipalpis species complex. Females of L. cruzi and L. longipalpis are morphologically indistinguishable and associated males must be examined carefully to confirm identifications. Chemical analysis hexane extracts of male L. cruzi has revealed the presence of a 9-methylgermacrene-B (C16), a homosesquiterpene (mw 218) previously shown to be the sex pheromone of one of the members of the L. longipalpis species complex.     

Quantitative ecological inventory of terra firme and várzea tropical forest on the Rio Xingu,Brazilian Amazon

Three hectares of Amazonian terra firme forest and an adjacent one-half hectare of várzea forest were quantitatively inventoried at O Deserto, on the Rio Xingu, Pará, Brazil. In the terra firme forest, 1420 individual trees greater than ten cm dbh, in 39 families and 265 species, were inventoried. In the várzea forest, there were 220 individual trees, in 17 families and 40 species.Cenostigma macrophyllum andOrbignya sp. were the most important species in the terra firme forest;Mollia lepidota andLeonia glycycarpa were most important in the várzea forest. Among one-hectare subplots of the total three-hectare terra firme sample, the number of trees ranged from 393 to 460, the number of families was a constant 33, and the number of species ranged from 118 to 162. This variation indicates that one-hectare samples are too small to be used to estimate the species richness of the total forest. The terra firme forest was richer in species and had a greater stature than the várzea forest.