共查询到20条相似文献,搜索用时 31 毫秒
1.
Ethylene regulation of fruit ripening: Molecular aspects 总被引:19,自引:0,他引:19
Progress in ethylene regulating fruit ripening concerning itsperception and signal transduction and expression of ACC synthaseand ACC oxidase genes is reviewed. ACC synthase and ACC oxidasehave been characterized and their genes cloned from various fruittissues. Both ACC synthase and ACC oxidase are encoded bymultigene families, and their activities are associated withfruit ripening. In climacteric fruit, the transition toautocatalytic ethylene production appears to be due to a seriesof events in which ACC sythase and ACC oxidase genes have beenexpressed developmentally. Differential expression of ACCsynthase and ACC oxidase gene family members is probably involvedin such a transition that ultimately controls the onset of fruitripening.In comparison to ACC synthase and ACC oxidase, less is knownabout ethylene perception and signal transduction because of thedifficulties in isolating and purifying ethylene receptors orethylene-binding proteins using biochemical methods. However, theidentification of the Nr tomato ripening mutant as anethylene receptor, the applications of new potent anti-ethylenecompounds and the generation of transgenic fruits with reducedethylene production have provided evidence that ethylenereceptors regulate a defined set of genes which are expressedduring fruit ripening. The properties and functions of ethylenereceptors, such as ETR1, are being elucidated.Application of molecular genetics, in combination withbiochemical approaches, will enable us to better understand theindividual steps leading from ethylene perception and signaltransduction and expression of ACC synthase and ACC oxidase genefamily member to the physiological responses. 相似文献
2.
3.
与授粉有关的因子调节的乙烯生物合成基因在兰花花器官中的表达 总被引:2,自引:0,他引:2
分析了与授粉有关的因子调节的ACC合酶和ACC氧化酶基因在朵丽蝶兰(DoritaenopsishybridaHort.)花中的表达。生长素和乙烯均可诱导ACC合酶和ACC氧化酶的mRNA在花器官中积累。然而,去雄却不能诱导这两个基因在花器官中表达。生长素和乙烯所诱导的ACC合酶和ACC氧化酶的mRNA在花器官中的积累模式相似。原位杂交结果表明,生长素和乙烯处理后ACC氧化酶的mRNA在柱头的表皮和薄壁细胞中积累。根据ACC合酶和ACC氧化酶基因表达的结果,对生长素、乙烯和去雄在兰花授粉后乙烯生物合成过程中的作用进行了分析。 相似文献
4.
Mature citrus fruits, which are classified as non-climacteric, evolve very low amounts of ethylene during ripening but respond to exogenous ethylene by ripening-related pigment changes and accelerated respiration. In the present study we show that young citrus fruitlets attached to the tree produce high levels of ethylene, which decrease dramatically towards maturation. Upon harvest, fruitlets exhibited a climacteric-like rise in ethylene production, preceded by induction of the genes for 1-aminocyclopropane-1-carboxylate (ACC) synthase 1 (CsACS1), ACC oxidase 1 (CsACO1) and the ethylene receptor CsERS1. This induction was advanced and augmented by exogenous ethylene or propylene, indicating an autocatalytic system II-like ethylene biosynthesis. In mature, detached fruit, very low rates of ethylene production were associated with constitutive expression of the ACC synthase 2 (CsACS2) and ethylene receptor
CsETR1 genes (system I). CsACS1
gene expression was undetectable at this stage, even following ethylene or propylene treatment, and CsERS1 gene expression remained constant, indicating that no autocatalytic response had occurred. The transition from system II-like behavior of young fruitlets to system I behavior appears to be under developmental control.Abbreviations ACC
1-Aminocyclopropane-1-carboxylate
- CsACS1, CsACS2
ACC synthase
- CsACO1
ACC oxidase
- CsERS1, CsETR1
Ethylene receptors
- DAFB
Days after full bloom
- 1-MCP
1-Methylcyclopropene 相似文献
5.
Pollination of flowers initiates postpollination development in orchid ( Doritaenopsis hybrida Hort. ) flowers, including perianth senescence, stigma closure, and ovary development. Because ethylene is thought to play a key role in coordinating these developmental changes, the authors studied the temporal and spatial patterns of expression of genes encoding 1-aminocyclopropane-l-carboxylic acid (ACC) synthase and ACC oxidase following pollination-associated factor treatments in orchid flowers. Both ACC synthase and ACC oxidase mRNA accumulation in the various parts of the flowers is induced by auxin, and ethylene, but not by emasculation. The patterns of both ACC synthase and ACC oxidase mRNA accumulation are similar in all floral organs following auxin and ethylene treatments. Further, in situ hybridization analysis indicates that the ACC oxidase mRNA is localized in epidermal and parenchyma cells of the stigma after auxin and ethylene treatments. The putative roles of auxin, ethylene and emasculation are discussed in terms of the regulation of ACC synthase and ACC oxidase gene expression in flowers. 相似文献
6.
Ozone induction of ethylene emission in tomato plants: regulation by differential accumulation of transcripts for the biosynthetic enzymes 总被引:8,自引:1,他引:7
Jaana Tuomainen Christian Betz Jaakko Kangasjärvi Dieter Ernst Zu-Hua Yin Christian Langebartels Heinrich Sandermann Jr 《The Plant journal : for cell and molecular biology》1997,12(5):1151-1162
7.
Expression of ACC synthase is enhanced earlier than that of ACC oxidase during fruit ripening of mume (Prunus mume) 总被引:3,自引:2,他引:1
Mume (Japanese apricot: Prunus mume Sieb. et Zucc.) is a climacteric fruit that produces large amounts of ethylene as it ripens. Ripening is accompanied by marked increases in the activities of two ethylene-biosynthetic enzymes, namely, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase. To study the molecular aspects of ripening of mume, we isolated cDNA clones for proteins that we considered likely to be involved in the biosynthesis and perception of ethylene during ripening, namely, ACC synthase, ACC oxidase and the ethylene receptor. Northern blotting analysis revealed the markedly increased expression of ACC synthase prior to that of ACC oxidase and the increase in ethylene production during ripening. Overall, the levels of the mRNAs for the genes corresponded closely to the levels of activity of the ethylene-biosynthetic enzymes. Exposure of mature green mume fruit to ethylene for 12 h induced strong expression of ACC synthase, as well as of ACC oxidase. Wounding of the pericarp of mume fruit induced the expression of ACC synthase but not of ACC oxidase. The rate of ethylene production increased only slightly after wounding. These results suggest that expression of the genes for ACC synthase and ACC oxidase must be activated sequentially for maximum production of ethylene during ripening of mume fruit and that several mechanisms regulate the expression of ethylene-biosynthetic genes during ripening. 相似文献
8.
Ethylene biosynthetic genes are differentially expressed during carnation (Dianthus caryophyllus L.) flower senescence 总被引:2,自引:0,他引:2
Ethylene production and expression patterns of an 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (CARAO1) and of two ACC synthase (EC 4.4.1.14) genes (CARACC3 and CARAS1) were studied in floral organs of cut carnation flowers (Dianthus caryophyllus L.) cv. White Sim. During the vase life and after treatment of fresh flowers with ethylene, production of ethylene and expression of ethylene biosynthetic genes first started in the ovary followed by the styles and the petals. ACC oxidase was expressed in all the floral organs whereas, during the vase life, tissue-specific expression of the two ACC synthase genes was observed. After treatment with a high ethylene concentration, tissue specificity of the two ACC synthase genes was lost and only a temporal difference in expression remained. In styles, poor correlation between ethylene production and ACC synthase (CARAS1) gene expression was observed suggesting that either activity is regulated at the translational level or that the CARAS1 gene product requires an additional factor for activity.Isolated petals showed no increase in ethylene production and expression of ethylene biosynthetic genes when excised from the flower before the increase in petal ethylene production (before day 7); showed rapid cessation of ethylene production and gene expression when excised during the early phase of petal ethylene production (day 7) and showed a pattern of ethylene production and gene expression similar to the pattern observed in the attached petals when isolated at day 8. The interorgan regulation of gene expression and ethylene as a signal molecule in flower senescence are discussed. 相似文献
9.
Diazocyclopentadiene (DACP), a competitive ethylene action inhibitor binds irreversibly to the ethylene receptor to reduce tissue responses to ethylene. Tomato fruit (Lycopersicon esculentum Mill cv lsquo;Rondellorsquo;) were treated with DACP at the mature green stage. Ethylene biosynthesis and respiration rate were depressed. Color changes from green to red were delayed. Compared to the control, ACC content increased and ACC oxidase activity in vivo decreased in DACP-treated fruit. Thus, decrease of ethylene production caused by DACP treatment was due to the reduction of ACC oxidase activity. The decline in ripening subsequently recovered after DACP treatment. Results from the Northern analysis for gene expression of ACC synthase and ACC oxidase, showed that expression of both genes declined in DACP-treated fruit, and then recovered. Therefore the recovery of ethylene production was due to the recovery in gene expression and activity of ACC oxidase. We conclude that the effects of DACP on ethylene biosynthesis are on expression of ACC synthase and ACC oxidase genes, and/or regulation of ACC oxidase activity. 相似文献
10.
11.
The authors investigated pollination-induced ethylene production and expression patterns of genes encoding 1-aminocyclopropane-l-carboxylate (ACC) synthase and ACC oxidase in orchid flowers (Doritaenopsis hybrida Hort. ). Following pollination both ACC synthase and ACC oxidase mRNAs were detected in the different organs of flowers, and the patterns of both ACC synthase and ACC oxidase mRNA accumulation were similar, mRNA accumulation of ACC synthase mRNA was more organ-specific than that of ACC oxidase mRNA. However, ACC oxidase mRNAs were much more abundant than ACC synthase mRNAs in the flower organs. 相似文献
12.
授粉诱导兰花花部乙烯生物合成基因在转录水平上的表达 总被引:4,自引:0,他引:4
朵丽蝶兰(Doritaenopsishybrida Hort.)的花授粉后,测定乙烯的产生,并分析授粉后花部各器官乙烯生物合成的ACC合成酶和ACC氧化酶两个基因转录水平上的表达。授粉后在花部均可探测到ACC合成酶和ACC氧化酶的m RNA。在花部不同器官之间,此两种酶的m RNA的积累水平均表现出一些差异。ACC合成酶的m RNA 积累与ACC氧化酶相比,具有更明显的特异性。而ACC氧化酶m RNA 的积累水平远比ACC合成酶高 相似文献
13.
14.
15.
The plant hormone ethylene triggers and enhanced ethylene synthesis in certain ripening fruits and senescing flowers. Unlike most carnation (Dianthus caryophyllus L.) cultivars exhibiting climacteric rise in ethylene production at the onset of senescence, cv. Sandrosa does not show this phenomenon naturally. In order to understand the mechanism of autocatalytic ethylene production, we exposed carnation flowers cv. Sandrosa to ethylene which resulted in an enhanced capacity for ethylene synthesis in the petals. A short time response of one hour was measured for an increase in ACC oxidase activity, about five hours in advance of an increase in ACC synthase activity and ethylene production. The observed enhancement was dependent on the presence of exogeneous ethylene, and could be partially inhibited by prior treatment of the petals with -amanitin or cycloheximide. The results of the present study suggest that in response to ethylene, activation of an existing enzyme is taking place first. This is followed by an increase in expression of ACC oxidase and ACC synthase mRNAs.Abbreviations ACC
1-aminocyclopropane-1-carboxylic acid
- DTT
dithiothreitol
- PMSF
phenyl-methylsulfonyl fluoride
- SAM
S-adenosyl-L-methionine 相似文献
16.
Ethylene production, ACC content, and ACC oxidase activity were determined in strawberry fruit harvested at different stages of development and in fruit harvested green and developed in vitro in solutions containing sucrose. In fruit harvested at progressive stages of development from green through full ripe, ethylene production and ACC oxidase activity decreased whereas ACC content increased between the white and pink stages. Fruit detached at the green stage and developed to full ripe by immersion of the cut pedicel in sucrose solutions exhibited an increase in ACC content, decreased ethylene production, and no change in ACC oxidase activity. Detached green fruit provided with sucrose containing 0.5 mM silver (STS) had elevated ethylene production and more ACC oxidase activity than did fruit incubated without the silver salt. Green fruit provided with sucrose containing 1 mM ACC showed markedly increased ACC content, ACC oxidase activity, and ethylene production. These increases were noted following 4 days incubation in ACC, and were more pronounced after 11 days, at which time fruit of all treatments had attained a full-ripe stage of development. Calyx tissue exhibited more ACC oxidase activity, less ACC content, and similar ethylene production compared with receptacle tissue. ACC synthase could not be detected in fruit harvested at different developmental stages or in fruit detached and developed in vitro.abbreviations ACC
1-aminocyclopropane-1-carboxylic acid
- HQS
8-hydroxyquinoline hemisulfate
- SAM
S-adenosyl methionine
- STS
silver thiosulfate 相似文献
17.
18.
To investigate the relationship between methyl jasmonate (MeJA) and ethylene in leaf senescence, we studied the effects of
MeJA on ethylene production and ethylene biosynthetic enzyme activities in oat(Avena sativa L.) leaf segments incubated in darkness. MeJA promoted dark-induced senescence judged from the contents of chlorophyll and
protein, and increased ethylene production 6 times of the control. MeJA also increased the activities of ethylene biosynthetic
enzymes, 1-aminocyclopropane carboxylic acid (ACC) synthase and ACC oxidase as compared to control. In MeJA-treated leaf segments,
ACC synthase activity reached its maximum level at 24 h of incubation and ACC oxidase activity peaked at 6 h of incubation.
Aminoethoxyvinylglycine (AVG) and Co2+, inhibitors of ACC synthase and ACC oxidase respectively, reduced MeJA-induced ethylene production. They also delayed leaf
senescence that was promoted by the treatment of MeJA. From these results, we can suggest that MeJA increased the activities
of ACC synthase and ACC oxidase, these increased activities lead to increase in ethylene production and this increased ethylene
production might promote dark-induced leaf senescence. 相似文献
19.
Zheng Y Wolff W Crosby M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,105(2-3):397-403
We investigated the genetics of ethylene biosynthesis and its linkage to the RFLPs of the ACC oxidase and synthase genes in melon ( Cucumis melo L.). The results suggested that the A(0) and B(0) fragments of RFLP-MEL1 of the ACC oxidase gene were two alleles from a single locus, as were the B and C fragments of RFLP-MEACS1 of the ACC synthase gene. The B(0) allele seemed to be partially dominant over the A(0) allele, whereas B and C alleles appeared to map to quantitative trait loci (QTLs), which most likely contributed to ethylene production. Both RFLPs were linked to ethylene production rates, but they were not linked to each other. The interaction effects of the ACC oxidase and synthase genes on ethylene production were revealed by segregation of RFLP-MEL1 and RFLP-MEACS1. The results of single-copy-reconstruction assays suggested that the ACC oxidase gene is a single copy, whereas the ACC synthase gene is a component of a multigene family in the melon genome. The abscission phenotype appeared to be controlled by an independent locus, with the abscission (full-slip) allele dominant over the non-abscission (not full-slip) allele. These results may facilitate efforts toward mapping the quantitative trait loci (QTLs) of ethylene production. The RFLPs may be used in marker-assisted selection in developing melons with a more-desirable low ethylene production rate for enhancing postharvest storage life. 相似文献
20.