共查询到20条相似文献,搜索用时 15 毫秒
1.
Virtual microscopy as an enabler of automated/quantitative assessment of protein expression in TMAs 总被引:1,自引:1,他引:0
Conway C Dobson L O'Grady A Kay E Costello S O'Shea D 《Histochemistry and cell biology》2008,130(3):447-463
Tissue Microarrays facilitate high-throughput immuohistochemistry; however, there are key bottlenecks apparent in their analysis, particularly when conducting microscope-based manual reviews. Traditionally Tissue Microarray assessments were performed using a microscope where results were either transcribed or dictated and subsequently entered into flat-file spreadsheets. This process is labour intensive, prone to error and negates the advantages of the high-throughput Tissue Microarray format. In addition, human interpretations of staining intensity parameters are highly subjective and therefore prone to inter- and intra-observer variability. The advent of Virtual Slides has permitted the review of tissue slides across the Internet. In addition, this new technology enables the creation of software solutions to assist in the manual and automated review of Tissue Microarrays, through the use of computer aided image analysis. There are numerous academically developed and commercially available applications which assist in Tissue Microarray reviews; functionality of these systems range in complexity and application domains. The review which follows describes these systems and outlines technical considerations to be assessed when deciding on a Tissue Microarray workflow solution. 相似文献
2.
Cell and Tissue Banking - Homografts have long been used for right ventricular outflow tract (RVOT) reconstruction. Tissue banks struggle to meet the clinical demand of tissue, with insufficient... 相似文献
3.
Davoudi Pahnekolayi Mahboubeh Tehranifar Ali Samiei Leila Shoor Mahmoud 《Plant Cell, Tissue and Organ Culture》2019,137(2):265-274
Plant Cell, Tissue and Organ Culture (PCTOC) - Tissue culture and micrografting techniques are known as effective procedures to produce healthy rose rootstocks and high quality scions. In order to... 相似文献
4.
A A Gage 《Cryobiology》1979,16(1):56-62
Experiments were performed correlating tissue temperature and tissue impedance in the course of freezing canine skin and palate. Tissue impedance rose gradually to high levels as the tissue froze. Tissue temperature of ?26 ° to ?30 °C corresponded closely to tissue impedance of 1 MΩ. Tissue impedance of 5 to 10 MΩ corresponded closely to temperatures of ?40 °C and colder. These observations indicated that the measurement of tissue impedance was related to tissue temperature and suggested that either or both could be used to predict tissue destruction in cryosurgery. 相似文献
5.
Ruth M. Warwick Robert Parker John N. Kearney Scott A. Brubaker 《Cell and tissue banking》2010,11(2):107-154
The 17th Annual Meeting of the European Association of Tissue Banks (EATB) was held in Edinburgh in November 2008 together
with the British Association for Tissue Banking (BATB) and received excellent evaluation by its participants. The meeting
included, for the first time, a cardiovascular symposium co-organised by EATB and BATB together with the American Association
of Tissue Banks (AATB). This was a first in terms of collaboration for the three associations. The many professionals involved
in organising the meeting created an excellent clinical and scientific programme including regulatory issues and featured,
also for the first time three interactive workshops about clinical donor cases, quality management and cardiovascular allografts. 相似文献
6.
Lacinova Z Dolinkova M Haluzikova D Krajickova J Haluzik M 《Molecular biotechnology》2008,38(3):195-201
Aim Comparison of manual and automatic (MagNA Pure) isolation methods of total RNA from adipose tissue with respect to its
quality and recovery factor. Material 120 human subcutaneous adipose tissue samples (about 100 mg/sample) were collected from patients during surgical operations.
The tissue sample was stabilized in RNAlater (QIAGEN GmbH, Germany). Methods Total RNA was extracted by the following kits: Rneasy Protect Mini, Rneasy Lipid Tissue (QIAGEN GmbH, Germany) and MagNA
Pure Compact RNA Isolation (Tissue) for MagNA Pure Compact Instrument (Roche Diagnostics GmbH, Germany). Results The average RNA yields with Rneasy Lipid Tissue kits were about two-fold higher in comparison with the Rneasy Protect
Mini kit. When the MagNA Pure Compact System was used, RNA yields from the same sample were more uniform compared with manual
systems. It was also more convenient and less time-consuming than the manual approach. No DNA contamination of total RNA samples
was detected except for samples isolated by Rneasy Protect Mini Kit. Conclusion Rneasy Lipid Tissue Kit and MagNA Pure Compact RNA Isolation Kit (Tissue) provide RNA samples of high quantity, purity
and PCR amplificability. RNA samples are suitable for further processing using methods of molecular biology. 相似文献
7.
The establishment of a Tissue Bank and the science of Tissue Banking in South Africa started in the 1960s and is still developing. This article describes the development and growth of Tissue Banking in South Africa. The current emphasis is to supply viable bone products that have been produced under the best possible quality controlled circumstances; with the collaboration between different Organ Donation Organisations. Through collaboration, a dramatic increase in the number of donors was noted over the years. Concurrently, there was a dramatic increase in the usage of different allograft products and now necessitates the development of new graft materials for expanding patient options. As an ongoing concern, the Tissue Bank in South Africa experienced an ever increase in costs to enhance quality/safety controls: increase in historical patient information, documentation and serological testing in a population struggling to control HIV. To date, the South African Tissue Bank has not experienced any untoward patient incidence since the 1960s and currently is getting over 500 donors per year. 相似文献
8.
Leite Cristiana Torres Ferreira Darley Aparecido Tavares Vieira Ariane Tonetto Praça-Fontes Milene Miranda Ferreira Adésio Carvalho Carlos Roberto Clarindo Wellington Ronildo 《Plant Cell, Tissue and Organ Culture》2019,136(3):549-560
Plant Cell, Tissue and Organ Culture (PCTOC) - Tissue culture in Passiflora has emerged as a strategy to propagate species with agronomic relevance, which is the main focus of most in vitro... 相似文献
9.
IAEA/NUS Distance Learning Diploma Training Course for Tissue Bank Operators – Past, Present and Future 总被引:1,自引:2,他引:1
National University Hospital (NUH) Tissue Bank as the Regional Training Centre for Asia Pacific Region provided National University of Singapore (NUS) Diploma Course in Tissue Banking - a long distance diploma course since 1997. To date, five batches have participated - 94 tissue bank operators. Sixty-three tissue bank operators have convocated with NUS Diploma in Tissue Banking.From Regional Co-operative Agreement (RCA) Project, RAS 7/008 technology transfer was effected to Latin America and to Africa.A Memorandum of Understanding has been signed between International Atomic Energy Agency (IAEA) and NUS in July 2002 making Singapore the International Training Centre. An Internet NUS Diploma Course in Tissue Banking has been developed by IAEA and NUS. The first on-line diploma course will be launched in 2003. 相似文献
10.
The impact of the International Atomic Energy Agency (IAEA) program on radiation and tissue banking in India 总被引:1,自引:0,他引:1
The banking of tissues such bone and skin began in India in the 1980s and 1990s. Although eye banking started in 1945 there
was little progress in this field for the next five decades. As part of the IAEA/RCA program to use ionising radiation for
the sterilisation of biological tissues in Asia and the Pacific Region, the Tata Memorial Hospital (TMH) in 1986 decided to
set up a tissue bank in Mumbai funded by the Government of India. The TMH Tissue Bank became operational in January 1988,
and stands as a pioneering effort in the country to provide safe, clinically useful and cost-effective human allografts for
transplantation. It uses the IAEA International Standards on Tissue Banking. All the grafts are sterilised terminally by exposure
to a dose of 25 kGy of gamma radiation, which has been validated as recommended by the IAEA Code of Practice for the Radiation
Sterilisation of Tissues Allografts: Requirements for Validation and Routine Control. The TMH Tissue Bank is registered with
the Maharashtra State Health Authorities, and in May 2004, it became India’s first Tissue Bank to receive ISO 9001:2000 certification
of its Quality Management System. From 1989 to September 2007, the TMH Tissue Bank has supplied 11,369 allografts to 310 surgeons
operating in 69 hospitals in Mumbai and 56 hospitals in other parts of India. These numbers have been limited by difficulties
with the retrieval of tissues from deceased donors due to inadequate resources and tissue donation policies of hospitals.
As the Government of India representative in the IAEA program, the TMH Tissue Bank has promoted and co-coordinated these activities
in the country and the development of tissue banks using radiation sterilisation of tissue grafts. Towards this end it has
been engaged in training personnel, drawing up project proposals, and supporting the establishment of a Tissue Retrieval Centre
in Mumbai. Currently it networks with the Zonal Transplant Co-ordination Centre of the Government of Maharashtra, and the
newly instituted National Deceased Donor Transplantation Network, which will work with the Government of India to set up rules
and regulations for organ and tissue donation and transplantation. 相似文献
11.
Examination of tissue cyst formation by Toxoplasma gondii in cell cultures using bradyzoites, tachyzoites, and sporozoites 总被引:2,自引:0,他引:2
D S Lindsay J P Dubey B L Blagburn M Toivio-Kinnucan 《The Journal of parasitology》1991,77(1):126-132
Tissue cyst formation by a goat isolate (GT-1) of Toxoplasma gondii was examined in bovine monocyte, human fetal lung, and Madin-Darby bovine kidney cell cultures. Transmission electron microscopy (TEM) and cat feeding studies indicated that tissue cysts were present in all 3 cell lines examined. Tissue cysts were first seen 3 days postinoculation (PI) using TEM. Standard cell culture procedures were used and no additional condition was needed to induce tissue cyst formation. Cats fed cell cultures excreted T. gondii oocysts in their feces 5-7 days PI. These oocysts caused lethal infections in mice. Tissue cysts were produced in cell cultures regardless if the initiating inoculum consisted of bradyzoites, sporozoites, or a mixture of bradyzoites and tachyzoites. Tissue cyst formation has been followed through 40 subpassages of infected cells. By TEM tissue cysts still were present after 40 passages, but when 40th-passaged cultures were fed to cats, oocytsts were not excreted. This indicates that the parasite had become oocystless after repeated passage in vitro. 相似文献
12.
Yu-Min Zhang Jian-Ru Wang Nai-Li Zhang Xiao-Ming Liu Mo Zhou Shao-Ying Ma Ting Yang Bao-Xing Li 《Cell and tissue banking》2014,15(3):291-296
Before 1986, the development of tissue banking in China has been slow and relatively uncoordinated. Under the support of International Atomic Energy Agency (IAEA), Tissue Banking in China experienced rapid development. In this period, China Institute for Radiation Protection tissue bank mastered systematic and modern tissue banking technique by IAEA training course and gradually developed the first regional tissue bank (Shanxi Provincial Tissue Bank, SPTB) to provide tissue allograft. Benefit from training course, SPTB promoted the development of tissue transplantation by ways of training, brochure, advertisement and meeting. Tissue allograft transplantation acquired recognition from clinic and supervision and administration from government. Quality system gradually is developing and perfecting. Tissue allograft transplantation and tissue bank are developing rapidly and healthy. 相似文献
13.
Vojácek J Dusek J Bis J Stásek J Blazek M 《Physiological research / Academia Scientiarum Bohemoslovaca》2008,57(1):1-5
Tissue factor is a cell surface protein that is expressed constitutively by monocytes, macrophages and fibroblasts, but also by some other cells in response to a variety of stimuli. The main function of the tissue factor is to form a complex with factor VII/VIIa that converts factors IX and X to their active forms. Tissue factor is also involved in the pathophysiology of systemic inflammatory disorders, coagulopathies, atherosclerotic disease, tumor angiogenesis and metastasis. Increased tissue factor expression either locally in the coronary plaques or systematically on circulating blood elements of patients with acute coronary syndromes may be responsible for increased thrombin generation, thus leading to platelet activation and fibrin formation. Tissue factor therefore plays a pivotal role in the initiation of thrombotic complications in patients with coronary artery disease. 相似文献
14.
European Association of Tissue Banks Standards for Cryopreserved Cardiovascular Tissue Banking 总被引:1,自引:0,他引:1
The Cardiovascular Tissue Banking Standards are designed as an addition to the General Standards of the European Association of Tissue Banks to provide a minimum acceptable level for the donation, processing, storage, testing, labelling and distribution of cardiac tissue throughout Europe. The aim is that all heart valve banks in Europe should work to these Standards so that heart valves can be exchanged between countries without having to check the individual protocols of the donor processing facility. The writing of the Standards has been performed by the Heart Valve Council of EATB with input from cardiac surgeons. It is proposed that once the Standards are accepted they will form the document on which EATB may accredit tissue banks in the future and may form the basis on which National Legislation for Tissue Banking is based. 相似文献
15.
Influence of Tissue Density-specific Mechanical Properties on the Scaling of Plant Height 总被引:6,自引:1,他引:5
Tissue density, 相似文献
16.
The 3rd Workshop on Heritable Disorders of Connective Tissue was held at the National Institutes of Health from 16th to 18th November, 2000. The Workshop was sponsored by the National Institute of Arthritis and Musculoskeletal and Skin Diseases, NIH Office of Rare Diseases, March of Dimes, Coalition for Heritable Disorders of Connective Tissue, and the Foundation for Basic Cutaneous Research. It was supported by specific grants R13 AR46912 (US Public Health Service) and 4-FY00-4511 (March of Dimes Birth Defects Foundation). The Workshop was divided into six sessions, featuring 29 invited presentations. In addition to the invited participants, more than eighty guests (scientists, NIH staff, and members of the Coalition for Heritable Disorders of Connective Tissue) attended. 相似文献
17.
American Association of Tissue Banks: A Historical Reflection Upon Entering the 21st Century 总被引:1,自引:0,他引:1
Joyce MJ 《Cell and tissue banking》2000,1(1):5-8
The American Association of Tissue Banks (AATB) is a scientific, not-for-profit, peer-group organization founded in 1976 to facilitate the provision of transplantable tissues of uniform high quality in quantities sufficient to meet national needs. The Association was created well before there was governmental oversight of tissue banking. The organization consists of individuals involved in tissue banking, medical users, and scientists in the field. Current structure consists of a 13-member Board of Governors with specific subgroups including Musculoskeletal, Reproductive, Skin, Tissue Bank Councils and Council of Accredited Tissue Banks. A historical review shows the evolution from development of guidelines to publication of standards, and from an inspection conducted by peers to one conducted by an independent, trained professional inspector. Association growth and historical accomplishments are highlighted. 相似文献
18.
Cell and Tissue Research - 相似文献
19.
The purpose of this case report is to illustrate the research potential of the tissue obtained from a single donor referred
to the Peterborough Hospitals NHS Trust (PHNHST) Research Tissue Bank. Tissue retrieval was done 19 hours after death and
453 tissue units processed on site were despatched to 20 mainly commercial client research organisations.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
20.
Plant Cell, Tissue and Organ Culture (PCTOC) - 相似文献