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OsDREB genes in rice,Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression 总被引:49,自引:0,他引:49
Dubouzet JG Sakuma Y Ito Y Kasuga M Dubouzet EG Miura S Seki M Shinozaki K Yamaguchi-Shinozaki K 《The Plant journal : for cell and molecular biology》2003,33(4):751-763
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拟南芥非生物胁迫应答基因表达的调节子研究概况 总被引:3,自引:0,他引:3
分子生物学研究表明,植物中由诸如干旱、高盐和低温等环境胁迫因子诱导的几个基因具有多种功能。大多数干旱应答基因是由植物激素脱落酸(ABA)诱导的,但也有少数基因例外。对模式植物拟南芥基因表达中的干旱应答基因的分析表明,至少存在4个独立调节系统(调节子)。对典型胁迫诱导表达的一些基因中启动子的顺势作用元件和影响这些基因表达的转录子也已进行了分析。已经分离出与脱水效应元件/C重复序列(DRE/CRT)顺势作用元件结合的转录因子,并命名为DRE结合蛋白1/C重复序列结合因子(DREB1/CBF)和DRE结合蛋白2(DREB2)。在转基因拟南芥植株中,DREB1/CBF过量表达可增加其抗寒、抗旱和抗盐碱的能力。DREB1/CBF基因已成功地在许多不同作物中得到应用,从而提高作物对非生物胁迫的耐受性。与胁迫反应相关的其他转录因子的研究也正在取得进展。 相似文献
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Role of soybean GmbZIP132 under abscisic acid and salt stresses 总被引:5,自引:0,他引:5
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Guoyun Xu Yanchun Cui Manling Wang Mingjuan Li Xuming Yin Xinjie Xia 《Molecular breeding : new strategies in plant improvement》2014,34(3):1055-1064
Salinity is a major environmental stress that limits agricultural production and geographical distribution of plants. In a previous study, it has been shown that OsMsr9 was induced by cold, drought and heat stresses. However, functions of OsMsr9 at physiological and molecular levels are still unknown. Here, we report that OsMsr9 plays roles in salt tolerance in plants. Quantitative real-time PCR (qPCR) analysis revealed that OsMsr9 was also rapidly and strongly induced by salt stress. Overexpression of OsMsr9 in Arabidopsis and rice showed enhanced salt stress tolerance displaying increased shoot and root elongation, higher survival rates in transgenic plants compared with wild type. OsMsr9 might act as a positive regulator of plant salt tolerance with reinforced expression of stress-related genes, such as RD29A, DREB2A and RAB18 in transgenic plants under salt conditions. Furthermore, transgenic plants accumulated more compatible solutes (proline and soluble sugar) and low level of malondialdehyde, alleviating the changes in reactive oxygen species. These results indicate that OsMsr9 could be a useful gene in developing transgenic crops with enhanced salt tolerance. 相似文献
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Soybean DREB1/CBF‐type transcription factors function in heat and drought as well as cold stress‐responsive gene expression 下载免费PDF全文
Satoshi Kidokoro Keitaro Watanabe Teppei Ohori Takashi Moriwaki Kyonoshin Maruyama Junya Mizoi Nang Myint Phyu Sin Htwe Yasunari Fujita Sachiko Sekita Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《The Plant journal : for cell and molecular biology》2015,81(3):505-518
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银新杨中与DRE元件结合的转录因子的克隆及鉴定分析 总被引:9,自引:0,他引:9
DREB类转录因子特异地与DRE元件结合,在植物感受非生物逆境(干旱、高盐和低温)胁迫时,激活一系列逆境应答基因的表达。我们选用银新杨(Populus alba×P. alba var. pyramidalis)为材料,通过PCR和同源EST搜索的方法克隆得到了一个类DREB的基因,命名为PaDREB2。酵母单杂交实验表明,该基因编码的蛋白能特异地与DRE元件结合并激活下游报告基因的表达。用RT-PCR的方法研究了PaDREB2的表达模式,结果表明PaDREB2受低温、干旱和高盐的胁迫诱导。 相似文献
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DREB1A是DREB转录因子的一员,它们都含有一段与DNA结合的保守区域,由58个氨基酸组成,称为EREBP/AP2结构域(EREBP/AP2 domain)。一个DREB转录因子可以调控多个与植物干旱、高盐及低温耐性有关的功能基因表达。从拟南芥中克隆了DREB1A转录因子基因,成功构建了DREB1A基因的原核表达载体,转化E.coliBL21(DE3),经1 mmol/L IPTG诱导表达获得了DREB1A蛋白,但以包涵体形式存在。为以后深入研究DREB转录因子与DRE顺式作用元件相互作用的具体机制奠定了基础。 相似文献
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