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1.
Radiation at the red end of the visible spectrum is active inreducing the extent of the first synchronous division in explantsremoved from Jerusalem artichoke tubers and cultured in a liquidmedium containing 2,4-D. The inhibitory effect of short redlight exposures may be overcome by subsequent irradiation withfar red light. However, prolonged exposures to red light mayonly partially be reversed by far red light. The addition ofcoconut milk to the medium completely overcomes the effect ofshort light exposures but cannot prevent the effect of prolongedlight treatments. These results are consistent with a hypothesiswhich postulates that a phytochrome-mediated reaction initiatesa chain of events which results in the depletion of an essentialmetabolite, also present in coconut milk, below a critical levelrequired for cell division. There is evidence to suggest thatthe essential metabolite is L-phenylalanine. 相似文献
2.
Inhibition of an early event in the cell division cycle of Escherichia coli by FL1060, an amidinopenicillanic acid. 总被引:32,自引:20,他引:12
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Analysis of exponential and synchronous cultures of Escherichia coli B/r after the addition of FL1060 indicates a block point for division by this agent some 15 to 20 min before the end of the preceding cell division cycle, a time corresponding to the beginning of the C period of the cell division cycle. Morphological examination of FL1060-treated synchronous cultures of E. coli /r was consistent with inhibition by FL1060 of a very early event in the cell division cycle. This event appears to be essential for normal cell surface elongation in a rod configuration. Temporary treatment of synchronous cultures of E. coli B/r with FL1060 resulted in division delay, the extent of which was a function of the duration of exposure to FL1060. However, even after relatively long times of FL1060 treatment the delayed divisions were still synchronous. Although FL1060 had no direct effect on deoxyribonucleic acid (DNA) synthesis, the synchronous delayed division occuring after temporary treatment with FL1060 were accompanied by a delay in the attainment of resistance of cell division to inhibitors of DNA, ribonucleic acid, and protein synthesis. These results suggest aht an FL1060-sensitive event initiates at the beginning of the C period of the cell division cycle of E. coli and is responsible for normal cell elongation. This cell elongation pathway procedes independently of DNA synthesis, but there is an interaction between this pathway and termination of a round of DNA replication in which a normal rod configuration is necessary to allow a signal for cell division to be generated upon completion of DNA replication. 相似文献
3.
Pollen Ultrastructure in Anther Cultures of Nicotiana tabacum: III . THE FIRST SP.OROPHYTIC DIVISION
Ultrastructure of embryogenic tobacco pollen was examined atthe first division of the vegetative cell. Structurally emptyzones formed before division by the degradation of gametophyticcytoplasm were rapidly re-populated with ribosomes, mitochondria,dictybsomes, and organelles interpreted as lipid centres. Thelatter appeared to be active in synthesis, and were associatedwith a vesicular component. Starch accumulation also began atthe first division in plastids carried over from the mothercell. Each daughter cell possessed a typical primary wall confluentwith the inner layers of the intine. Plasmodesmata traversedthe wall between the two cells. The cells were either equalor unequal; equal divisions occurred in both the longitudinaland transverse planes. In many proembryoids, the generativecell showed signs of degeneration. The cell was either attachedto the intine or detached from it. Detached cells sometimesdivided. The data, are discussed in relation to other embryogenicand morphogenic systems induced from normally quiescent or highlydifferentiated cells. 相似文献
4.
Potassium Uptake in Synchronous and Synchronized Cultures of Escherichia coli 总被引:6,自引:1,他引:5
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Criteria are presented for distinguishing between synchronous and synchronized cultures (natural vs. forced synchrony) on the basis of characteristics of growth and division during a single generation. These criteria were applied in an examination of the uptake of potassium during the cell growth and division cycle in synchronous cultures and in a synchronized culture of Escherichia coli. In the synchronous cultures the uptake of 42K doubled synchronously with cell number, corresponding to a constant rate of uptake per cell throughout the cell cycle. In the synchronized culture, uptake rates also remained constant during most of the cycle, but rates doubled abruptly well within the cycle. This constancy of 42K uptake per cell supports an earlier interpretation for steady-state cultures that uptake is limited in each cell by a constant number of functional sites for binding, transport, or accumulation of compounds from the growth medium, and that the average number of such sites doubles late in each cell cycle. The abrupt doubling of the rate of uptake of potassium per cell in the synchronized culture appears because of partial uncoupling of cell division from activation or synthesis of these uptake sites. 相似文献
5.
Multicellular Pollen Formation in Cultured Barley Anthers: II. THE A, B, AND C PATHWAYS 总被引:1,自引:1,他引:0
Patterns of nuclear and cellular division leading to the formationof haploid, non-haploid, and mixoploid multicellular pollengrains in cultured anthers of Hordeum vulgare cv. Sabarlis aredescribed. The vegetative cell divides either by repeated unequalhaploid divisions (A pathway) or by synchronous nuclear divisionsaccompanied by fusion and variable wall formation (non-haploidand mixoploid). The divisions are random and unorganized. Thegenerative cell degenerates or is integrated into the vegetativecomponent either before or after division has commenced. Priorfusion (C pathway) leads to binucleate or bicellular diploidunits partitioned by a straight transverse wall. These binucleateand bicellular units are cytologically indistinguishable fromother diploid units derived from spores which do not form agenerative cell (B pathway). Division of the bicellular unitsmay be random and unorganized, but in some instances the twocells divide synchronously and in a highly ordered manner yieldingembryonic-like octants of cells. The diploid binucleate unitsgenerate still higher ploidies with or without wall formation. The significance of the various segmentation patterns is discussedin relation to previous concepts of pollen-callus formation. 相似文献
6.
In Xenopus and Drosophila, the nucleocytoplasmic ratio controls many aspects of cell-cycle remodeling during the transitory period that leads from
fast and synchronous cell divisions of early development to the slow, carefully regulated growth and divisions of somatic
cells. After the fifth cleavage in sea urchin embryos, there are four populations of differently sized blastomeres, whose
interdivision times are inversely related to size. The inverse relation suggests nucleocytoplasmic control of cell division
during sea urchin development as well. To investigate this possibility, we developed a mathematical model based on molecular
interactions underlying early embryonic cell-cycle control. Introducing the nucleocytoplasmic ratio explicitly into the molecular
mechanism, we are able to reproduce many physiological features of sea urchin development. 相似文献
7.
Effect of Light on Cell Division in Developing Callus Cultures 总被引:3,自引:0,他引:3
Explants removed from the Jerusalem artichoke tuber and exposedto white light in the presence of 2, 4-D, when cultured in liquidmedia, exhibit much smaller dividing populations than similartissue not exposed to light. White light, which is effectiveonly in the presence of 2, 4-D and during the period beforethe onset of DNA replication, is required only in small amountsto promote a maximum effect, although inhibition of cell divisionwas never complete Light does not interfere with the timingof the cell cycle but exerts an influence on the size of thedividing population. The results presented are consistent witha hypothesis which postulates that a substance or substancesessential for cell division is reduced in amount by exposureto light. The extent of the first synchronous division is probablytherefore determined by the supply of this substance. 相似文献
8.
Asymmetric cell divisions occur repeatedly during plant development, but the mechanisms by which daughter cells are directed to adopt different fates are not well understood [1,2]. Previous studies have demonstrated roles for positional information in specification of daughter cell fates following asymmetric divisions in the embryo [3] and root [4]. Unequally inherited cytoplasmic determinants have also been proposed to specify daughter cell fates after some asymmetric cell divisions in plants [1,2,5], but direct evidence is lacking. Here we investigate the requirements for specification of stomatal subsidiary cell fate in the maize leaf by analyzing four mutants disrupting the asymmetric divisions of subsidiary mother cells (SMCs). We show that subsidiary cell fate does not depend on proper localization of the new cell wall during the SMC division, and is not specified by positional information acting on daughter cells after completion of the division. Instead, our data suggest that specification of subsidiary cell fate depends on polarization of SMCs and on inheritance of the appropriate daughter nucleus. We thus provide evidence of a role for unequal inheritance of an intracellular determinant in specification of cell fate after an asymmetric plant cell division. 相似文献
9.
Filtration of late log-phase cultures of Bacillus megaterium ATCC 19213 grown on defined sucrose salts medium (SS) or SS plus glutamate medium (SSG) through nine layers of Whatman no. 40 filter paper in a fritted-glass disc Büchner funnel resulted in filtrates containing cells which showed synchronous growth and proceeded to sporulation. SS cells completed one synchronous division after filtration; sporulation ensued after the cessation of growth. SSG cells completed two synchronous divisions and sporulation occurred during the second division. A high degree of synchrony of vegetative growth of SSG cells was evident by the stepwise pattern of growth, by the doubling of cell numbers at each division, the high division index, and by the rapid formation of sporulation cell types and homogeneity of cell types in the filtered cultures when compared with asynchronous cultures. Because the described system gives both good growth and sporulation synchrony, the method should be useful in delineating early events in sporulation and their regulation. 相似文献
10.
J D Lathia M Hitomi J Gallagher S P Gadani J Adkins A Vasanji L Liu C E Eyler J M Heddleston Q Wu S Minhas A Soeda D J Hoeppner R Ravin R D G McKay R E McLendon D Corbeil A Chenn A B Hjelmeland D M Park J N Rich 《Cell death & disease》2011,2(9):e200
Malignant gliomas contain a population of self-renewing tumorigenic stem-like cells; however, it remains unclear how these glioma stem cells (GSCs) self-renew or generate cellular diversity at the single-cell level. Asymmetric cell division is a proposed mechanism to maintain cancer stem cells, yet the modes of cell division that GSCs utilize remain undetermined. Here, we used single-cell analyses to evaluate the cell division behavior of GSCs. Lineage-tracing analysis revealed that the majority of GSCs were generated through expansive symmetric cell division and not through asymmetric cell division. The majority of differentiated progeny was generated through symmetric pro-commitment divisions under expansion conditions and in the absence of growth factors, occurred mainly through asymmetric cell divisions. Mitotic pair analysis detected asymmetric CD133 segregation and not any other GSC marker in a fraction of mitoses, some of which were associated with Numb asymmetry. Under growth factor withdrawal conditions, the proportion of asymmetric CD133 divisions increased, congruent with the increase in asymmetric cell divisions observed in the lineage-tracing studies. Using single-cell-based observation, we provide definitive evidence that GSCs are capable of different modes of cell division and that the generation of cellular diversity occurs mainly through symmetric cell division, not through asymmetric cell division. 相似文献
11.
Summary The pattern of divisions in the thallus ofPelvetia compressa embryos was determined with respect to the embryonic growth axis. To detect all possible division planes, embryos were viewed from two vantages which permitted observations of (1) the thallus pole and (2) the longitudinal embryonic profile. Following formation of rhizoid and thallus cells by any asymmetrical division transverse to the embryonic axis that is established prior to any divisions, the thallus cell divided twice along the embryonic axis (axial divisions) in orthogonal planes, and then divided transverse to the growth axis. This division pattern produced an eight-cell thallus with four cells in each of two layers. The spatial relation between gravity and the first axial division was investigated, and gravity was found to have little effect on the alignment of this division. The reproducible pattern of divisions in the thallus indicates spatial control of spindle positioning.Abbreviations ASW
artificial seawater
- AF
after fertilization 相似文献
12.
DONALD J. REINHARDT 《The Journal of eukaryotic microbiology》1968,15(3):480-493
SYNOPSIS. The mycetozoan genus Echinosteliopsis, resembling the myxomycete Echinostelium in some of its features, is described. The single species, E. oligospora Reinhardt & Olive, forms small sporocarps which consist of a basal disk, stalk and a sporangium with only 1–8 spores. Spores form progressively, not simultaneously, by segmentation. The spores germinate to release non-flagellate amebae which, in liquid, assume a characteristic broad, fan shape. Each ameba has one or more nuclei. The nucleus is distinctive because of refractile, globular to elongate peripheral bodies which cytochemical tests indicate to be primarily RNA. At the time of nuclear division the characteristic RNA bodies disappear and, as observed with the phase microscope and in stained preparations, optically dense material accumulates in the middle area of the nucleus. Threads, either a spindle or actual chromatin, can be seen attached to the nuclear membrane. The threads separate to opposite poles as the nucleus elongates. During this division process the nuclear membrane apparently remains intact. Synchronous binucleate divisions, as well as a tripolar nuclear division, have been observed. Uninucleate and synchronous binucleate divisions may or may not be followed by cytokinesis. The absence of cell division after nuclear division leads to the production of cells with varying numbers of nuclei. Nuclear divisions in early sporangial stages and in spores have not been observed. The spores are uni- to multinucleate. In 8-spored sporangia and in most 4-spored sporangia there is a characteristic small “stalk spore” at the apex of the stalk. The stalk spore germinates slowly, if at all, but the larger spores germinate readily. No evidence of a sexual process has been found. 相似文献
13.
Vittoria Nuti Ronchi Lucia Giorgetti Mariagrazia Tonelli Guido Martini 《Plant Cell, Tissue and Organ Culture》1992,30(2):115-119
Carrot cell lines W1 and W2 express permanently in culture a meiotic-like phenotpe, with apparent pairing and chiasmata formation comparable to meiosis during carrot microsporogenesis. The variant lines also show several variants of division in relation to the presence or absence of cytokinesis, nuclear fusion or spindle disturbance.The meiotic-like divisions can also be found in the abnormal structures, which are regenerated from these spontaneous variant lines. A possible role of the chromosome reducing mechanisms on carrot embryogenesis capacity and somaclonal variability is postulated. 相似文献
14.
Effect of Ethylene on Cell Division and Deoxyribonucleic Acid Synthesis in Pisum sativum 总被引:9,自引:8,他引:1
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Ethylene and supraoptimal levels of 2,4-dichlorophenoxyacetic acid inhibit the growth of the apical hook region of etiolated Pisum sativum (var. Alaska) seedlings by stopping almost all cell divisions. Cells are prevented from entering prophase. The hormones also retard cell division in intact root tips and completely stop the process in lateral buds. The latter inhibition is reversed partially by benzyl adenine. In root tips and the stem plumular and subhook regions, ethylene inhibits DNA synthesis. The magnitude of this inhibition is correlated with the degree of repression of cell division in meristematic tissue, suggesting that the effect on cell division results from a lack of DNA synthesis. Ethylene inhibits cell division within a few hours with a dose-response curve similar to that for most other actions of the gas. Experiments with seedlings grown under hypobaric conditions suggest that the gas naturally controls plumular expansion and cell division in the apical region. 相似文献
15.
Namboori B. Raju 《Protoplasma》1984,121(1-2):87-98
Summary Mitotic division stages studied by light microscopy in differentNeurospora crassa cell types clearly resemble prophase, metaphase, anaphase, and telophase stages of higher eukaryotes. 1. When conidia are cultured in liquid medium containing 3.22 M ethylene glycol, they grow without cell division, forming giant spheres with multiple nuclei. In a few giant cells, nuclear numbers remain small (1 to 3) but the nuclei become very large. Seven large chromosomes are seen in some nuclei suggesting polyteny, 14 or more chromosomes are seen in other, very large nuclei, indicating polyploidy. Cell volume and nuclear volume are positively correlated in giant cells. Nuclear divisions are not synchronous within individual multinucleate giant cells. 2. Nuclear division stages were also observed in crosses heterozygous for the dominant mutant banana where haploid prefusion nuclei in late-forming croziers revert to mitosis. Swollen ascogenous hyphae become highly multinucleate after several rounds of mitosis. Mitosis is completely synchronous in nuclei of the same crozier cyst, providing replicate information for unambiguous identification of division stage. 3. Observations are also reported of mitosis in a cell-wall deficient slime strain. Previous observations on mitosis in large nuclei of the ascus are summarized for comparison. The nucleolus persists throughout mitosis in the giant cells, multinucleate reverted croziers, and in the cell-wall deficient slime strain. It is expelled from the dividing nuclei in the ascus. Spindles and spindle pole bodies, which are normally conspicuous in asci, are also seen in normal and reverted croziers, but they have not been clearly identified in the ethylene glycol-induced giant cells. 相似文献
16.
Anita Kulukian Elaine Fuchs 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2013,368(1629)
Asymmetric cell divisions (ACDs) result in two unequal daughter cells and are a hallmark of stem cells. ACDs can be achieved either by asymmetric partitioning of proteins and organelles or by asymmetric cell fate acquisition due to the microenvironment in which the daughters are placed. Increasing evidence suggests that in the mammalian epidermis, both of these processes occur. During embryonic epidermal development, changes occur in the orientation of the mitotic spindle in relation to the underlying basement membrane. These changes are guided by conserved molecular machinery that is operative in lower eukaryotes and dictates asymmetric partitioning of proteins during cell divisions. That said, the shift in spindle alignment also determines whether a division will be parallel or perpendicular to the basement membrane, and this in turn provides a differential microenvironment for the resulting daughter cells. Here, we review how oriented divisions of progenitors contribute to the development and stratification of the epidermis. 相似文献
17.
Hunter CT Kirienko DH Sylvester AW Peter GF McCarty DR Koch KE 《Plant physiology》2012,158(2):708-724
The Cellulose Synthase-Like D (CslD) genes have important, although still poorly defined, roles in cell wall formation. Here, we show an unexpected involvement of CslD1 from maize (Zea mays) in cell division. Both division and expansion were altered in the narrow-organ and warty phenotypes of the csld1 mutants. Leaf width was reduced by 35%, due mainly to a 47% drop in the number of cell files across the blade. Width of other organs was also proportionally reduced. In leaf epidermis, the deficiency in lateral divisions was only partially compensated by a modest, uniform increase in cell width. Localized clusters of misdivided epidermal cells also led to the formation of warty lesions, with cell clusters bulging from the epidermal layer, and some cells expanding to volumes 75-fold greater than normal. The decreased cell divisions and localized epidermal expansions were not associated with detectable changes in the cell wall composition of csld1 leaf blades or epidermal peels, yet a greater abundance of thin, dense walls was indicated by high-resolution x-ray tomography of stems. Cell-level defects leading to wart formation were traced to sites of active cell division and expansion at the bases of leaf blades, where cytokinesis and cross-wall formation were disrupted. Flow cytometry confirmed a greater frequency of polyploid cells in basal zones of leaf blades, consistent with the disruption of cytokinesis and/or the cell cycle in csld1 mutants. Collectively, these data indicate a previously unrecognized role for CSLD activity in plant cell division, especially during early phases of cross-wall formation. 相似文献
18.
J Lamprecht 《Cell biology international reports》1987,11(6):449-455
The morphology and orientation of cytokinetic figures were studied in the rat corneal epithelium by light microscopy using serial semi-thick plastic sections. It has been found that the mitotising cell of the basal epithelial layer produces either a pair of morphologically similar daughter cells--equivalent or symmetrical division or a pair of morphologically dissimilar cells--differential or asymmetrical division. The equivalent divisions are horizontally orientated, whereas the differential divisions are oblique or vertical in relation to the basement membrane. 相似文献
19.
The TORMOZ gene encodes a nucleolar protein required for regulated division planes and embryo development in Arabidopsis
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Griffith ME Mayer U Capron A Ngo QA Surendrarao A McClinton R Jürgens G Sundaresan V 《The Plant cell》2007,19(7):2246-2263
Embryogenesis in Arabidopsis thaliana is marked by a predictable sequence of oriented cell divisions, which precede cell fate determination. We show that mutation of the TORMOZ (TOZ) gene yields embryos with aberrant cell division planes and arrested embryos that appear not to have established normal patterning. The defects in toz mutants differ from previously described mutations that affect embryonic cell division patterns. Longitudinal division planes of the proembryo are frequently replaced by transverse divisions and less frequently by oblique divisions, while divisions of the suspensor cells, which divide only transversely, appear generally unaffected. Expression patterns of selected embryo patterning genes are altered in the mutant embryos, implying that the positional cues required for their proper expression are perturbed by the misoriented divisions. The TOZ gene encodes a nucleolar protein containing WD repeats. Putative TOZ orthologs exist in other eukaryotes including Saccharomyces cerevisiae, where the protein is predicted to function in 18S rRNA biogenesis. We find that disruption of the Sp TOZ gene results in cell division defects in Schizosaccharomyces pombe. Previous studies in yeast and animal cells have identified nucleolar proteins that regulate the exit from M phase and cytokinesis, including factors involved in pre-rRNA processing. Our study suggests that in plant cells, nucleolar functions might interact with the processes of regulated cell divisions and influence the selection of longitudinal division planes during embryogenesis. 相似文献
20.
Spatial patterning of vulval precursor cell fates is achieved through a different two-stage induction mechanism in the nematode Oscheius/Dolichorhabditis sp. CEW1 compared with Caenorhabditis elegans. We therefore performed a genetic screen for vulva mutants in Oscheius sp. CEW1. Most mutants display phenotypes unknown in C. elegans. Here we present the largest mutant category, which affects division number of the vulva precursors P(4-8).p without changing their fate. Among these mutations, some reduce the number of divisions of P4.p and P8.p specifically. Two mutants omit the second cell cycle of all vulval lineages. A large subset of mutants undergo additional rounds of vulval divisions. We also found precocious and retarded heterochronic mutants. Whereas the C. elegans vulval lineage mutants can be interpreted as overall (homeotic) changes in precursor cell fates with concomitant cell cycle changes, the mutants described in Oscheius sp. CEW1 do not affect overall precursor fate and thereby dissociate the genetic mechanisms controlling vulval cell cycle and fate. Laser ablation experiments in these mutants reveal that the two first vulval divisions in Oscheius sp. CEW1 appear to be redundantly controlled by a gonad-independent mechanism and by a gonadal signal that operates partially independently of vulval fate induction. 相似文献