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1.
A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex André cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and
Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1–1.0 mg/l), α-naphthalene
acetic acid (0.01–0.2 mg/l), thidiazuron (0.5–2.0 mg/l), 6-benzylaminopurine (0.5–1.0 mg/l), and kinetin (0.5–1.0 mg/l)] were
tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots.
Basal medium, as well as the combination and concentration of hormones applied, had a significant effect on callus formation,
shoot regeneration and adventitious root formation. Winarto and Teixeira-1, an original basal medium containing 0.01 mg/l
α-naphthalene acetic acid, 0.5 mg/l thidiazuron and 1.0 mg/l 6-benzylaminopurine was suitable for callus formation while an
improved basal medium i.e., New Winarto–Teixeira-3 supplemented with 0.25 mg/l 2,4-dichlorophenoxy acetic acid, 0.02 mg/l
α-naphthalene acetic acid, 1.5 mg/l thidiazuron and 0.75 mg/l 6-benzylaminopurine enhanced callus formation. High shoot regeneration
and multiplication was also possible on New Winarto–Teixeira-3. Shoots formed a strong adventitious root system on New Winarto–Teixeira-3
containing 0.2 mg/l α-naphthalene acetic acid and 1.0 mg/l kinetin. Plantlets that varied in size and performance were successfully
acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that
34 were haploid (n = 14–18), 15 aneuploid (n = 20–26), 126 diploid (n = 28–34) and 5 triploid (n = 45–57). The potential use of this protocol for developing half-anther culture of other Anthurium species or cultivars is discussed. 相似文献
2.
Efficient plant regeneration was achieved from callus derived from immature-cotyledon explants of oleaster (Elaeagnus angustifolia L.). Calli were obtained on MS media containing 3% sucrose and different concentrations of TDZ. The highest rate of green,
compact and nodular callus was formed on MS medium supplemented with 1 mg/l of TDZ. Shoot organogenesis was achieved when
the callus was transferred onto MS media containing 3% sucrose and BA alone (05–4 mg/l) or BA (0.5 and 1 mg/l) combined with
NAA or IAA (0.5 and 1 mg/l). Maximum organogenesis was obtained with 1 mg/l BA in combination with 0.5 mg/l NAA. Rooting of
the shoots was achieved on MS medium supplemented with 0.2 mg/l IBA. Regenerated plantlets were acclimatized and successfully
transplanted to soil. 相似文献
3.
We present efficient protocols for the regeneration of fertile plants from corm explants of Hypoxis hemerocallidea Fisch. & C. A. Mey. landrace Gaza, either by direct multiple shoot formation or via shoot organogenesis from corm-derived calluses. The regeneration efficiency depended on plant growth regulator concentrations
and combinations. Multiple direct shoot formation with high frequency (100% with 5–8 shoots/explant) was obtained on a basal
medium (BM) supplemented with 3 mg/l kinetin (BM1). However, efficient indirect regeneration occurred when corm explants were
first plated on callus induction medium (BM2) with high kinetin (3 mg/l) and naphthalene acetic acid (NAA 1 mg/l), and then
transferred to shoot inducing medium (BM3) containing BA (1.5 mg/l) and NAA (0.5 mg/l). Shoot regeneration frequency was 100%
and 30–35 shoots per explant were obtained. The regenerated shoots were rooted on a root inducing medium (BM4) containing
NAA (0.1 mg/l). Rooted plantlets were transferred to the greenhouse. The regenerants were morphologically normal and fertile.
Flow cytometric analyses and chloroplast counts of guard cells suggested that the regenerants were diploid. Efficient cloning
protocols described here, have the potential not only to substantially reduce the pressure on natural populations but also
for wider biotechnological applications of Hypoxis hemerocallidea—an endangered medicinal plant. 相似文献
4.
S. Zhihui M. Tzitzikas K. Raemakers M. Zhengqiang R. Visser 《In vitro cellular & developmental biology. Plant》2009,45(6):776-782
Pea (Pisum sativum L. cv. Espace) seeds directly cultured on thidiazuron (TDZ)-containing medium formed high numbers of shoots. The number of
shoots per seedling depended on the concentration and duration of the TDZ treatment. The best treatment was 12-wk incubation
on MS medium supplemented with 4 mg/l TDZ followed by 4-wk culture on MS medium supplemented with 0.5 mg/l benzylaminopurine
(BA) and produced more than 400 shoots/seedling. Isolated shoots rooted at a high frequency on MS medium containing 2–3 mg/l
indole-3-butyric acid and 2 mg/l α-naphthalene acetic acid. In addition to the formation of shoots, bud-containing tissues
(BCT) were formed at the cotyledonary nodes, shoot nodes, tendrils, stipules, and internodes. The BCT from the cotyledonary
nodes and the shoot nodes was maintained in its pure state on MS medium supplemented with 4 mg/l TDZ by repeated culture.
Shoot development was accomplished when the BCT were left on MS medium supplemented with 4 mg/l TDZ without subculture prior
to transfer onto MS medium supplemented with 0.5 mg/l BA. 相似文献
5.
Pradeep C. Deo Robert M. Harding Mary Taylor Anand P. Tyagi Douglas K. Becker 《Plant Cell, Tissue and Organ Culture》2009,99(1):61-71
Callus was initiated in three different “esculenta” taro cultivars by culturing corm slices in the dark on half-strength MS
medium supplemented with 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) for 20 days followed by subculture of all corm slices
to half-strength MS medium containing 1.0 mg/l thidiazuron (TDZ). Depending on the cultivar, 20–30% of corm slices produced
compact, yellow, nodular callus on media containing TDZ. Histological studies revealed the presence of typical embryogenic
cells which were small, isodiametric with dense cytoplasms. Somatic embryos formed when callus was transferred to hormone-free
medium and ~72% of the embryos germinated into plantlets on this medium. Simultaneous formation of roots and shoots during
germination, and the presence of shoot and root poles revealed by histology, confirmed that these structures were true somatic
embryos. Plants derived from somatic embryos appeared phenotypically normal following 2 months growth in a glasshouse. This
method is a significant advance on those previously reported for the esculenta cultivars of taro due to its efficiency and reproducibility. 相似文献
6.
Mei-Chun Lu 《Plant Cell, Tissue and Organ Culture》2004,78(1):93-96
High frequency plant regeneration was induced from protocorm-derived callus cultured on half-strength of Murashige—Skoog medium
with 2,4-dichlorophenoxyacetic acid (2,4-D, 0–5 mg l−1) and 1-phenyl-3-(1,2,3-thiadiazol-5-yl, 0–1 mg l−1) urea (TDZ) in the dark. Twelve totipotent callus lines were selected within 76 callus lines regenerated on half-strength
of Murashige—Skoog (MS) medium with 0.5 mg l−1 TDZ. The proliferation rate was 4–5-fold in fresh weight after 30 days of culture on half-strength MS medium containing 5
mg l−1 2,4-D and 0.5 mg l−1 TDZ in the dark. The maximum number of shoot buds generated by 0.01 g callus explant was 134 after 4 months of culture. These
calli were regenerated to plantlets via protocorm-like bodies (PLBs) after 75–150 days of culture. The shoots, with two true
leaves, were transferred to hormone-free medium, rooting and eventually formed plantlets. Totipotent callus lines of Pleione formosana Hayata have been successfully established in this study. 相似文献
7.
In vitro plant regeneration from callus of niger (Guizotia abyssinica Cass.) cv. Sahyadri 总被引:1,自引:0,他引:1
Callus formation was achieved with root, hypocotyl, and cotyledon explants of niger (Guizotia abyssinica Cass.) cultivar Sahyadri on Murashige and Skoog medium containing 0.5 mg l–1
β-indoleacetic acid + 1.5 mg l–1 6-benzylaminopurine (BAP). Hypocotyl and cotyledon-derived calli when transferred onto a medium with 0.5 mg l–1 BAP produced an average of 12–32 shoots/ callus culture. The callus retained its potential for shoot regeneration for more
than 19 months. The shoots formed an extensive root system and were transferred to pots kept in a greenhouse, where the survival
rate was 98%. The plantlets flowered in vitro if transfer to fresh medium or to soil was delayed by 40–50 days. All regenerants
were diploid with 2n=30.
Received: 13 March 1997 / Revision received: 17 May 1997 / Accepted: 5 July 1997 相似文献
8.
A regeneration system was developed for Prunus serotina from a juvenile (F) and two mature genotypes (#3 and #4). Adventitious shoots regenerated from leaves of in vitro cultures
on woody plant medium with thidiazuron (TDZ) and naphthaleneacetic acid (NAA). The best regeneration for genotype F (91.4%)
was observed on medium with 9.08 μM TDZ and 1.07 μM NAA. The highest mean number of shoots (8.2) was obtained on medium containing
9.08 μM TDZ and 0.54 μM NAA. Genotype #3 had the highest regeneration (41.7%) with a mean number of shoots (4.8) on 9.08 μM
TDZ and 1.07 μM NAA, whereas genotype #4 had a 38.8% regeneration with a mean of 3.3 shoots. Genotype #4 had the highest mean
number of shoots (4.8) on 4.54 μM TDZ and 1.07 μM NAA. Silver thiosulphate at 60 or 80 μM increased the percent regeneration
of the mature genotypes #3 (75%) and #4 (58%). Adventious shoots were rooted (70–76%) and rooted plantlets survived after
acclimatization to the greenhouse. The effect of kanamycin concentration on adventitious shoot regeneration was also evaluated. 相似文献
9.
S. C. Debnath 《In vitro cellular & developmental biology. Plant》2009,45(2):122-128
An efficient system to regenerate shoots on excised leaves of greenhouse-grown wild lowbush blueberry (Vaccinium angustifolium Ait.) was developed in vitro. The effect of thidiazuron (TDZ) on adventitious bud and shoot formation from apical, medial, and basal segments of the leaves
was tested. Leaf cultures produced multiple buds and shoots with or without an intermediary callus phase on 2.3–4.5 μM TDZ
within 6 wk of culture initiation. The greatest shoot regeneration came from young expanding basal leaf segments positioned
with the adaxial side touching the culture medium and maintained for 2 wk in darkness. Callus development and shoot regeneration
depended not only on the polarity of the explants but also on the genotype of the clone that supplied the explant material.
TDZ-initiated cultures were transferred to medium containing 2.3–4.6 μM zeatin and produced usable shoots after one additional
subculture. Elongated shoots were dipped in 39.4 mM indole-3-butyric acid powder and planted on a peat:perlite soilless medium
at a ratio of 3:2 (v/v), which yielded an 80–90% rooting efficiency. The plantlets were acclimatized and eventually established in the greenhouse
with 75–85% survival. 相似文献
10.
M. Koné E. M. Patat-Ochatt C. Conreux R. S. Sangwan S. J. Ochatt 《Plant Cell, Tissue and Organ Culture》2007,88(1):61-75
The morphogenetic competence of Bambara groundnut was assessed for different landraces, explant sources and media compositions.
With cotyledon explants, the best callusing occurred on a medium containing 3 mg/l BAP + 0.5 mg/l NAA, while roots were produced
with 3–5 mg/l BAP + 0.5 mg/l NAA. Shoots regenerated (∼6%) from cotyledons on media with BAP alone (3–5 mg/l) or combined
with 0.01–0.1 mg/l NAA. Flowers were regenerated on 5 mg/l BAP + 0.5 mg/l NAA, without any intervening callus phase. With
epicotyls, the highest callusing was on 3 mg/l BAP + 0.5 mg/l NAA, and shoots regenerated (15–20%) on 3 mg/l BAP alone or
with NAA at concentrations that depended on the landrace studied. Regenerated shoots rooted on hormone-free medium, and plants
transferred to the greenhouse were all morphologically normal and fertile. Flow cytometry showed that most regenerants were
diploid and in addition permitted to distinguish between landraces according to their relative nuclear DNA content. This is
the first report on de novo regeneration in vitro of Bambara groundnut, an important yet neglected legume crop. 相似文献
11.
Yaser Hassan Dewir Nisha Singh Shakira Shaik Ashley Nicholas 《In vitro cellular & developmental biology. Plant》2010,46(1):41-46
The present study reports a simple protocol for indirect shoot organogenesis and plant regeneration of Sutherlandia using rachis and stem segments. Different concentrations (0.0–68.08 μmol l−1) of thidiazuron (TDZ) were used for callus induction and shoot organogenesis. The highest percentage of callus formation
(97.5%) and the highest percentage of explants forming shoots (88.8%) were obtained from rachis explants cultured onto Murashige
and Skoog (MS) medium (Murashige and Skoog, Physiol. Plant. 15:473–495, 1962) supplemented with 45.41 μmol l−1 TDZ. Scanning electron microscopy demonstrated the early development of adventitious shoots derived from callus cultures.
Shoot clusters were further developed and grown in MS hormone-free medium. The presence of l-canavanine was determined by thin-layer chromatography and confirmed after column fractionation using silica gel and nuclear
magnetic resonance spectroscopy. Individual shoots were rooted on different concentrations and combinations of MS salt strength
and IBA. Half-strength MS salt medium supplemented with 24.6 μmol l−1 IBA was optimal for root induction in which 78% of shoots were rooted. The in vitro plants were successfully acclimatized in a growth chamber with a 90% survival rate. 相似文献
12.
Vijendra K. Sharma Robert Hänsch Ralf R. Mendel Jutta Schulze 《Plant Cell, Tissue and Organ Culture》2007,88(1):21-33
A successful regeneration system is presented for elite cultivars in barley and wheat based on nodes. Nodal explants were
excised from in vitro and ex vitro grown plants. A combination of 8.28 μM 4-amino-3,5,6-trichloropicolinic acid and 4.54 μM
to 22.71 μm thidiazuron (TDZ) used in MS-based medium containing 60 g l−1 maltose favoured induction of clumps of multiple shoots/buds with or without callus formation in the primary cultures. Within
8–10 weeks upon further subcultures, the proliferation into callus with rapid and continuously forming adventitious buds containing
clusters of meristemoids, termed meristematic bulk tissue (MBT) was obtained. Lowering the levels of growth regulators resulted
in redifferentiation of shoots, which elongated, rooted, developed into morphologically normal plants and set seeds normally.
With a frequency ranging between 37 and 82% the nodes raised from in vitro grown plants were proliferated into MBT independent
of TDZ concentration, cultivar and species. The average number of shoots per responding node in different cultivars was 7–15
in barley and 1–6 in wheat after 12–14 weeks. Nodes from greenhouse grown plants mainly responded for callus formation with
poor development of MBT. 相似文献
13.
Feng Liu Li–Li Huang Yang-Li Li Poula Reinhoud Maarten A. Jongsma Cai-Yun Wang 《Plant Cell, Tissue and Organ Culture》2011,104(1):111-117
For the first time, an in vitro regeneration protocol of Hydrangea macrophylla ‘Hyd1’ was developed. Effects of different plant growth regulators (PGRs) on shoot regeneration were investigated jointly
with selecting optimal basal media and cefotaxime concentrations. The highest frequency of shoot organogenesis (100%) and
mean number of shoots per explant (2.7) were found on Gamborg B5 basal medium supplemented with 2.25 mg/l 6-benzyladenine
(BA), 0.1 mg/l Indole-3-butyric acid (IBA), 100 mg/l cefotaxime and 30 g/l sucrose solidified by 7 g/l agar. Regenerated shoots
were rooted by culturing on perlite plus half strength liquid B5 basal medium with 0.5 mg/l NAA. Rooted plantlets were transplanted
to the greenhouse with 100% survival rate. Genetic stability of 32 plantlets (one mother plant and 31 regenerants) was assessed
by 44 ISSR markers. Out of 44 ISSR markers, ten markers produced clear, reproducible bands with a mean of 5.9 bands per marker.
The in vitro regeneration protocol is potentially useful for the genetic transformation of Hydrangea macrophylla ‘Hyd1’. 相似文献
14.
A novel protocol for callus-mediated shoot regeneration was established for an important medicinal and ornamental plant native
to South China, Curcuma kwangsiensis, using shoot base sections excised from seedlings in vitro as explant sources. The frequency of callus formation reached
91% for explants cultured on MS medium containing 1.4 μM TDZ, 4.4 μM BA and 2.3 μM 2,4-D. 8.2 shoots per callus was achieved
on MS medium supplemented with 1.4 μM TDZ, 17.8 μM BA and 2.7 μM NAA. Single shoots transferred into MS medium free of plant
growth regulator rooted well. Regenerated plants acclimatized ex vitro at 100%, and grew vigorously under shaded greenhouse
conditions. 相似文献
15.
Xiuli Shen Michael E. Kane Jianjun Chen 《In vitro cellular & developmental biology. Plant》2008,44(4):282-288
The capacity for indirect shoot organogenesis of leaf and root explants of four Dieffenbachia cultivars were examined on a modified Murashige and Skoog (MS; Physiol Plant 15:473–495, 1962) medium supplemented with different plant growth regulators in 112 combinations. Callus formation was only observed from
leaf explants on MS supplemented with 1–10 μM thidiazuron (TDZ) and 0.5–1.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D) regardless
of cultivars. The combination of 5 μM TDZ and 1 μM 2,4-D resulted in the greatest callus formation frequency among the four
cultivars tested. Significant differences in callus and shoot formation from leaf explants were also observed among cultivars.
Cultivars Camouflage, Camille, Octopus, and Star Bright produced green nodular, brown nodular, yellow friable, and green compact
calli with corresponding maximum callus formation frequencies of 96%, 62%, 54%, and 52%, respectively. A maximum of 6.7 shoots/callus
was observed in cv. Camouflage, followed by cvs. Camille and Star Bright at 3.7 and 3.5, respectively. Calli of cv. Octopus
displayed no capacity for shoot organogenesis. Regardless of cultivar, callus formation was not observed on root explants.
Regenerated shoots were successfully acclimatized in a shaded greenhouse condition with 100% survival. 相似文献
16.
A highly efficient and reproducible method of in vitro propagation using meristematic explants has been developed for castor.
Embryo axes and shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with 0.5–10.0 mg/l of adenine, N6-benzyladenine (BA), kinetin (Kn), thiadiazuron (TDZ) and zeatin. TDZ (1.0–10.0 mg/l) gave the maximum number of shoots (37.8–40.0)
from embryo axes, while BA (2.0 mg/l) was found superior to other cytokinins for obtaining the highest number of shoots (46.7)
from the shoot apex. Adenine and Kn at all of the tested concentrations resulted in low proliferation rates from embryo axes.
The carryover effect of the cytokinins was tested by subculturing proliferating shoot cultures from various media onto the
medium fortified with 0.5 mg/l BA. There was no significant influence of the cytokinins on subsequent proliferation from the
two explant types except for TDZ with embryo axes. The number of shoots from TDZ-habituated embryo axes ranged between 36.0
and 81.7, while it varied from 5.7 to 22.0 and 3.7 to 28.3 in axillary buds and embryo axes, respectively, on the other media.
For elongation of shoots, gibberellic acid (GA3) (0.1–1.0 mg/l) was added to the medium supplemented with 0.2–0.5 mg/l BA. Incorporation of GA3 (0.1 mg/l) significantly enhanced the frequency of elongated shoots but drastically reduced the multiplication ability. Hence,
proliferating shoot clusters were periodically transferred to the medium supplemented with 0.5 and 0.2 mg/l BA for further
multiplication and elongation. Well-developed shoots were rooted on half-strength MS medium supplemented with 1.0 mg/l indole-3-butyric
acid. The rooted plantlets were acclimatized with more than 60% success.
Received: 17 June 1997 / Revision received: 3 September 1997 / Accepted: 20 September 1997 相似文献
17.
Mahendra Laxman Ahire Savaliram Goga Ghane Vinayak Haribhau Lokhande Penna Suprasanna Tukaram Dayaram Nikam 《In vitro cellular & developmental biology. Plant》2011,47(4):488-495
Uraria picta is extensively used in the Asian traditional systems of medicine. Overexploitation of the species for preparation of the
drug Dashmula has led to the plant becoming rare and endemic. In the present investigation, an efficient micropropagation
protocol has developed from leaf-derived callus of U. picta. Among the various concentrations of cytokinins (6-benzyladenine—BA; kinetin—Kin; and thidiazuron—TDZ) used, a significantly
higher number of shoots per culture (58.8 ± 0.8) was observed on Murashige and Skoog (MS) medium fortified with 4.44 μM BA.
The shoot regeneration frequency was sustained upon transfer to the same fresh medium at 4-wk intervals over a period of 2 yr.
The medium containing various concentrations of auxins (α-napthalene acetic acid (NAA) or indole-3-acetic acid (IAA)) showed
callus interspersed root formation; however, MS basal medium containing 3% sucrose revealed direct root induction from in vitro raised shoots. The acclimatized in vitro grown plants showed almost 98% survival upon transfer to soil in earthen pots and grown ex vitro. Randomly amplified polymorphic DNA analysis of 25 arbitrarily selected regenerants and mother plants revealed 100% uniformity
and true-to-type nature of the regenerants. Methanolic extracts of callus showed strong antibacterial activity against pathogenic
bacteria as compared to leaf and root extracts of in vitro raised plants and wild plants, suggesting the presence of higher concentrations of active chemical constituents (isoflavanoids)
in callus cultures of U. picta. 相似文献
18.
Transgenic plants with the herbicide-resistance gene (bar gene) were obtained via organogenesis from isolated mesophyll protoplasts of Nierembergia repens after applying electroporation. Transient β-glucuronidase (GUS) activity of electroporated protoplasts assayed 2 days after
applying an electric pulse showed that optimum condition (transient GUS activity 319 pmol 4 MU/mg per min and plating efficiency
2.43%) for electroporation was 0.5 kV/cm in field strength and 100 μF in capacitance. The protoplasts electroporated with
the bar gene at this condition initiated formation of microcolonies on medium after 2 weeks. After 4 weeks of culture, equal volume
of fresh 1/2-strength Murashige and Skoog (MS) medium containing 0.2 mg/l bialaphos was added for selection of transformed
colonies. After 6 weeks of culture, growing colonies were transferred onto regeneration medium containing 1.0 mg/l bialaphos,
on which they formed adventitious shoots 1–2 months after electroporation. The adventitious shoots rooted easily after transfer
onto MS medium with bialaphos lacking plant-growth regulators. Transformation of these regenerants with the bar gene was confirmed by Southern analysis. Some of the transformants showed strong resistance to the application of bialaphos
solution at 10.0 mg/l. 相似文献
19.
Summary An improved protocol for shoot regeneration from hypocotyl segments of seedlings from open-pollinated seeds of lingonberry
(Vaccinium vitis-idaea L.) cultivars, ‘Ida’, ‘Splendor’, and ‘Erntesegen’, and a native clone from Newfoundland was developed. The effect of thidiazuron
(TDZ) on adventitious bud and shoot formation from apical, central, and basal segments of the hypocotyl was tested. Highly
regenerative callus was obtained from hypocotyl segments on modified Murashige and Skoog (MMS) medium containing 5–10 μM TDZ. A maximum of 10 buds and 12 shoots per apical segment for seedlings of cultivar ‘Ida’ regenerated on MMS containing
10 μM TDZ. Callus and bud regeneration frequency, callus growth, and number of buds and shoots per regenerating explant depended
not only on the specific segment of the hypocotyl, but also on parental genotype. Inhibition of shoot elongation by TDZ was
overcome by transferring shoot cultures to a shoot proliferation medium containing 1–2 μM zeatin. The optimal concentration of sucrose for shoot elongation was 20 gl−1. Shoots were rooted ex vitro on a 2 peat: 1 perlite (v/v) medium after dipping in 0.8% indole-3-butyric acid, and rooted plants acclimatized readily under
greenhouse conditions. 相似文献
20.
M. Gallo-Meagher R. G. English A. Abouzid 《In vitro cellular & developmental biology. Plant》2000,36(1):37-40
Summary Efficient shoot regeneration of sugarcane (Saccharum spp. hybrid cv. CP84-1198) from embryogenic callus cultures has been obtained using thidiazuron (TDZ). Callus was placed
on modified Murashige and Skoog (MS) medium containing 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D), or 9.3 μM kinetin and 22.3 μM naphthaleneacetic acid (NAA) and compared with the same MS medium supplemented with 0.5, 1.0, 2.5, 5.0 or 10.0 μMTDZ, A11 TDZ treatments resulted in faster shoot regeneration than the kinetin/NAA treatment, and more shoot production than
either the 2,4-D or kinetin/NAA treatments. Maximum response, as determined by total number of shoots (26 per explant) and
number of shoots greater than 1 cm (4 per explant) 4 wk after initiation, was obtained with 1.0 μM TDZ. The shoots rooted efficiently on MS medium supplemented with 19.7 μM indole-3-butyric acid (IBA). These results indicate that TDZ effectively stimulates sugarcane plant regeneration from embryogenic
callus, and may be suitable to use in genetic transformation studies to enhance regeneration of transgenic plants. 相似文献