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A single beta-globin locus control region element (5'' hypersensitive site 2) is sufficient for developmental regulation of human globin genes in transgenic mice. 总被引:3,自引:0,他引:3
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B J Morley C A Abbott J A Sharpe J Lida P S Chan-Thomas W G Wood 《Molecular and cellular biology》1992,12(5):2057-2066
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Kamiie K Yamashita T Taira H Kidou S Ejiri S 《Bioscience, biotechnology, and biochemistry》2003,67(7):1522-1529
Elongation factor 1 (EF-1) from the silk gland of Bombyx mori consists of four subunits: alpha (51 kDa), beta (26 kDa), gamma (49 kDa), and delta (33 kDa). The EF-1alpha subunit catalyzes the binding of aminoacyl-tRNA to the ribosome concomitant with the hydrolysis of GTP. The EF-1alpha-bound GDP is then exchanged for GTP by the EF-1betagammadelta complex. To facilitate analysis of the roles of the individual EF-1beta, gamma, and delta subunits in GDP/GTP exchange on EF-1alpha, we cloned the cDNAs for these subunits and expressed them in Escherichia coli. EF-1beta, EF-1gamma, and the carboxyl-terminal half of EF-1delta were expressed, purified, and examined for protein:protein interactions by gel filtration chromatography and by a quartz-crystal microbalance method. An 80-kDa species containing EF-1beta and gamma subunits in a 1:1 molar ratio was detected by gel filtration. A higher molecular weight species containing an excess of EF-1gamma relative to EF-1beta was also detected. The amino-terminal region of EF-1beta (amino acid residues 1-129) was sufficient for binding to EF-1gamma. The carboxyl-terminal half of EF-1delta did not appear to form a complex with EF-1gamma. 相似文献
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R C Hardison 《Molecular biology and evolution》1984,1(5):390-410
The members of the rabbit and human beta-like globin gene families have
been compared both by a computer-generated dot matrix graphical analysis of
each entire gene and by calculating divergences in the coding regions. The
rabbit-human gene pairs beta 4-epsilon, beta 3- gamma, psi beta 2-delta,
and beta 1-beta were identified as orthologous on the basis of sequence
similarities found in flanking and intervening sequences as well as by
quantitative divergence calculations. The orthologous genes are in the same
order on the chromosome in each species, which suggests that an ancestral
family with the arrangement 5'-epsilon-gamma-delta-beta-3' preceded the
mammalian radiation. Descendants of ancestral epsilon have diverged more
slowly than other beta-like genes and are expressed only in embryonic life.
Descendants of ancestral gamma and beta diverged at a higher rate and are
expressed at wider range of developmental times. Descendants of delta have
undergone nonreciprocal recombination at a high frequency and are often
pseudogenes. Paralogous comparisons among the rabbit beta-like globin genes
show that the beta 4-beta 3 and psi beta 2-beta 1 pairs are most similar
and that beta 4 and beta 3 are more closely related to beta 1 than to psi
beta 2. This fits with a branching pattern where the primordial beta split
into ancestral epsilon/gamma and delta/beta genes, which later split into
epsilon and gamma or delta and beta, respectively. Rabbit genes beta 4 and
beta 1 acquired similar 3' untranslated regions after the epsilon/gamma
split but prior to the mammalian radiation, presumably via a gene
conversion event. The 5' end of beta 2 apparently converted with beta 1
after the radiation, and afterward it became a pseudogene.
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Cloning, expression and chromosomal localization of a human testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene 总被引:1,自引:0,他引:1
6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2) is a bifunctional enzyme responsible for the synthesis and breakdown of Fru-2,6-P2, a key metabolite in the regulation of glycolysis. Several genes encode distinct PFK-2/FBPase-2 isozymes that differ in their tissue distribution and enzyme regulation. In this paper, we present the isolation of a cDNA from a human testis cDNA library that encodes a PFK-2/FBPase-2 isozyme. Sequencing data show an open reading frame of 1407 nucleotides that codifies for a protein of 469 amino acids. This has a calculated molecular weight of 54kDa and 97% similarity with rat testis PFK-2/FBPase-2, with complete conservation of the amino acid residues involved in the catalytic mechanism. Fluorescence in-situ hybridization (FISH) localized testis PFK-2/FBPase-2 gene (PFKFB4) in human chromosome 3 at bands p21-p22. A Northern blot analysis of different rat tissues showed the presence of a 2.4-kb mRNA expressed specifically in testis. In mammalian COS-1 cells, the human testis cDNA drives expression of an isozyme with a molecular weight of 55kDa. This isozyme shows clear PFK-2 activity. Taken together, these results provide evidence for a new PFK-2/FBPase-2 gene coding for a human testis isozyme. 相似文献
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R C Hardison 《The Journal of biological chemistry》1983,258(14):8739-8744