Snail基因及其蛋白、E-cadherin蛋白在胃癌中的表达及其临床意义

目的观察Snail mRNA及其蛋白、E-cadherin蛋白在胃癌组织中的表达及其与胃癌临床病理特征的关系,并探讨它们在胃癌发生、发展中的作用及其临床应用价值。方法收集96例手术切除胃癌标本,同时取80例癌旁组织作为对照。应用免疫组织化学S-P法检测胃癌组织、癌旁组织中snail蛋白、E-cadherin蛋白的表达;运用原位分子杂交技术检测胃癌组织、癌旁组织中Snail mRNA的表达。结果（1）Snail蛋白在胃癌组织阳性率（83.3%）显著高于癌旁组织（41.25%）（P〈0.05）;高、中分化组Snail蛋白阳性表达率显著低于低分化组（P〈0.05）;Snail蛋白的阳性表达率在乳头状腺癌、管状腺癌及低分化腺癌与黏液癌之间差异有显著性（P〈0.05）;Snail蛋白的表达与胃癌浸润深度、淋巴结转移及远处转移有关（P〈0.05）,与性别、年龄、肿瘤大小、肿瘤部位及临床分期无关（P〉0.05）;（2）胃癌组织中snail mR-NA的阳性率（76%）显著高于癌旁组织（30%）（P〈0.05）;高、中分化组Snail mRNA阳性表达率显著低于低分化组（P〈0.05）;Snail mRNA的阳性表达率在乳头状腺癌、管状腺癌及低分化腺癌与黏液癌之间差异有显著性（P〈0.05）;Snail mRNA的表达与浸润深度及淋巴结转移有关（P〈0.05）,与性别、年龄、肿瘤大小、肿瘤部位、临床分期及远处转移无关（P〉0.05）;（3）E-cadherin蛋白在胃癌组织阳性率（37.5%）显著低于癌旁组织（100%）（P〈0.05）;高、中分化组E-cadherin蛋白阳性率显著高于低分化组（P〈0.05）;E-cadherin蛋白阳性率在乳头状腺癌、管状腺癌及低分化腺癌与黏液癌之间差异有显著性（P〈0.05）;E-cadherin蛋白的表达与胃癌浸润深度、淋巴结转移、临床分期及远处转移有关（P〈0.05）,与性别、年龄、肿瘤大小、肿瘤部位均无关（P〉0.05）;（4）胃癌组织中snail mRNA和snail蛋白的表达呈正相关（r=0.594,P〈0.05）;Snail蛋白和E-cadherin蛋白的表达呈负相关（r=-0.234,P〈0.05）。结论（1）E-cadher-in蛋白低表达与Snail蛋白高表达可能是胃黏膜恶性转变以及胃癌发生浸润转移的重要生物学标志;联合检测E-cadherin蛋白与Snail蛋白对预测胃癌浸润转移有重要意义。（2）Snail蛋白可能在转录水平上调控E-cadherin蛋白的表达。     

转录因子Ets-1与MMP-9在胃癌中的表达及临床意义

目的观察转录因子Ets-1和MMP-9在胃癌组织中的表达;探讨两者在胃癌浸润转移中的作用、相互关系及意义。方法采用免疫组织化学S-P法检测97例胃癌及癌旁组织、28例正常胃黏膜组织中Ets-1和MMP-9的表达。结果胃癌组织中Ets-1和MMP-9的阳性表达率分别为74.2%(72/97)、75.3%(73/97),均明显高于癌旁组织(40.2%、18.6%)及正常胃黏膜组织(17.9%、14.3%)(P0.01);而在癌旁组织及正常胃黏膜组织中两者的表达差异无显著性(P0.05)。Ets-1和MMP-9的阳性表达率在乳头状腺癌、管状腺癌及低分化腺癌与黏液癌之间差异有显著性(P0.01)。Ets-1和MMP-9的高表达与胃癌浸润深度、淋巴结转移及临床分期有关(P0.01),与性别、年龄、肿瘤大小及肿瘤位置均无关(P0.05)。Ets-1和MMP-9的表达成正相关(r=0.700,P0.01)。结论Ets-1和MMP-9高表达与胃癌的浸润、转移密切相关;通过上调MMP-9的表达,可能是Ets-1促进胃癌浸润转移的机制之一。     

胃癌组织中MEG3基因甲基化状态及其临床意义

目的：探讨胃癌中MEG3基因差异性甲基化区域甲基化水平及其与MEG3表达之间的关系,并分析其临床病理意义。方法：利用荧光定量PCR检测38例胃癌组织及其对应癌旁正常组织中MEG3的表达水平,并利用甲基化特异性PCR检测MEG3基因差异性甲基化区域的甲基化水平。结果：①胃癌组织中MEG3表达水平明显低于正常癌旁对照组织（P〈0.05）;②胃癌组织MEG3差异性甲基化区域的甲基化率（21/38,55.3%）显著高于正常对照组织（10/38,26.3%,P〈0.05）③胃癌组织MEG3差异性甲基化区域的甲基化率在性别、年龄、发病部位的差异无统计学意义（P〉0.05）;在肿瘤的大小,淋巴结转移,浸润深度上差异有统计学意义（P〈0.05）。结论：胃癌组织中MEG3呈低表达水平,其差异性甲基化区域的甲基化与肿瘤的大小,淋巴结转移,浸润深度有关。     

胃癌组织中PTEN、VEGF表达与肿瘤新生血管形成

目的探讨胃癌组织中PTEN、vascular endothelial growthfactor（VEGF）基因表达及其与肿瘤侵袭转移的关系。方法用RT-PCR和免疫组化方法检测胃癌、淋巴结转移组织中PTEN、VEGF mRNA和蛋白表达;用CD34检测肿瘤细胞微血管数。结果PTEN和VEGF mRNA表达阳性率在正常胃黏膜为76.5%与0.0%、胃癌组织为30.9%与69.1%、淋巴结转移组织23.6%与74.5%;PTEN和VEGF蛋白阳性率在正常胃黏膜为76.5%与0.0%、胃癌组织27.9%与82.4%、淋巴结转移组织16.3%与91.0%;胃癌组织中新生血管呈浸润生长,以淋巴结转移组织中明显。胃癌组织PTEN mRNA和蛋白低于正常胃黏膜（P〈0.01）,VEGF高于正常胃黏膜（P〈0.01）,PTEN与VEGF表达负相关（P〈0.05）,VEGF表达与新生血管形成正相关（P〈0.05）。结论PTEN基因失活和VEGF的过表达与新生血管形成相关,可能是通过调节包括VEGF在内的血管生成因子而在血管形成中起作用。     

SNCG在胃癌中的表达及意义

目的：探讨SNCG在胃癌及正常胃粘膜组织中的蛋白和mRNA表达及其与临床病理特征的关系。方法：采用免疫组织化学SP法检测90例胃癌及40例正常胃粘膜组织中SNCG蛋白表达情况,同时应用RT-PCR检测29例胃癌及15例正常胃粘膜组织中SNCG mRNA的表达情况。结果：SNCG蛋白在胃癌组中的表达高于正常胃粘膜组（Uc=7.149,P〈0.05）,胃癌组中SNCG蛋白阳性表达与癌组织的浸润深度以及有无淋巴结转移有关（Uc=2.742,Uc,3.970,P均〈0.05）,而与患者的性别、年龄、及癌组织的分化程度无关。SNCG mRNA在胃癌组织中的表达量明显高于正常胃粘膜组织（t=4.399,P〈0.01）。结论：SNCG在胃癌组织中的高表达与胃癌发生发展及浸润转移密切相关,可能会成为胃癌早期发现、早期诊断以及判断转移、预后的重要参考指标。     

胃癌组织中的MUC1和VEGF表达及其意义

目的:检测胃癌组织中VEGF和MUC1的表达情况,研究二者与胃癌生物学行为之间的关系。方法:应用免疫组织化学SP法检测VEGF和MUC1在胃癌组织和癌旁组织中的表达情况。结果:胃癌组织中VEGF的阳性表达明显高于癌旁组织,两者之间差异存在统计学意义(P0.05),VEGF在胃癌组织中的表达与胃癌浸润深度、有无远处转移、有无淋巴结转移、TNM分期有关,之间差异存在统计学意义(P0.05);胃癌组织中MUC1的阳性表达明显高于癌旁组织,两者之间差异存在统计学意义(P0.05),MUC1在胃癌组织中的表达与分化程度、TNM分期、淋巴结转移、远处转移有关,差异有统计学意义(P0.05);胃癌患者组织VEGF与MUC1的表达水平呈正相关(r=0.210,P0.05)。结论:VEGF和MUC1在胃癌发生、发展和转移过程中起重要作用,可能成为检测胃癌的重要肿瘤标志物。     

MDM2在胃癌组织中的表达及与Hp-L型感染关系的研究

目的研究MDM2在胃癌组织中的表达意义及其与幽门螺杆菌L型(Helicobacter pylori-L,Hp-L)感染在胃癌发生中的关系。方法 (1)应用革兰染色法和免疫组化学法检测100例胃癌及对应的40例癌旁正常胃黏膜组织(对照组)中Hp-L型感染和MDM2蛋白的表达情况;(2)应用逆转录多聚酶链反应(RT-PCR)方法检测MDM2的mRNA在30例新鲜胃癌组织及其对应的癌旁正常胃粘膜组织(对照组)中的表达情况。结果 100例胃癌组织及对应的40例癌旁正常胃粘膜组织中MDM2阳性表达率为71.0%(71/100)和42.5%(17/40),可见MDM2在胃癌组织中高表达和在非胃癌组中低表达具有统计学意义(P0.05),且MDM2表达水平升高与肿瘤大小、浸润深度、淋巴结转移和TNM分期有关(P0.05),与性别、年龄无关(P0.05);RT-PCR显示,肿瘤组织MDM2的表达明显高于远端正常胃黏膜对照组织的表达量且差异明显(P0.01)。革兰染色和免疫组化两种方法检测Hp-L型结果具有一致性(P0.05),胃癌组织中Hp-L型感染阳性组的MDM2表达阳性率高于Hp-L型阴性组(P0.05),且Hp-L型阳性率和MDM2蛋白表达呈正相关(r=0.447,P0.05)。结论 MDM2蛋白在胃癌中呈高表达,且与胃癌的浸润、转移有关,其机制可能与Hp-L型感染有关。     

HIF-1α和VEGF—c在胃癌组织中的表达及临床意义研究

目的：探讨HIF-1α和VEGF—c在胃癌组织中的表达情况及临床病理意义。方法：选取2010年12月至2012年12月期间就诊于我院肿瘤外科需进行手术切除的胃癌标本及其配对的癌旁组织各73例进行研究分析,采用免疫组化SP（streptavidin perotridase）对胃癌组织以及癌旁组织中HIF-1α和VEGF-c的表达情况进行检测。结果：①HIF-1α和VEGF-c在癌旁组织几乎不表达,而在胃癌组织中的阳性表达率分别为83．56％、78．08％,显著高于癌旁组织,经分析,差异具有统计学意义（P〈0．05）;②HIF—hx和VEGF-c的阳性表达和浸润深度、淋巴结转移以及临床分期密切相关,差异具有统计学意义（P〈0．05）;③HIF-1α和VEGF-c在胃癌组织中的表达存在一定的相关性（r=0．654,P〈0．05）。结论：HIF．hx和VEGF-c的阳性表达可以作为胃癌侵袭转移的重要判定指标,这对于本病的临床治疗具有一定的指导性意义。     

H.pylori-L型感染及OPN表达与胃癌浸润转移的相关性研究

目的通过检测胃癌中幽门螺杆菌L型（Helicobacter pylori L-form,H.pylori-L型）感染以及骨桥蛋白（osteopontin,OPN）的表达情况,探讨H.pylori-L型在胃癌侵袭、转移中的作用。方法将胃癌BGC-823细胞与H.pylori-L型按不同比例共培养,细胞爬片进行免疫组化（SP法）检测BGC-823细胞OPN的表达情况。革兰染色及免疫组化检测120例胃癌组织中H.pylori-L型的感染情况。免疫组化检测胃癌组织中的OPN的表达。结果 （1）随着细菌比例增大,细胞爬片OPN阳性的胃癌BGC-823细胞数逐渐增加,各组之间差异有统计学意义（F=137.5,P〈0.01）。（2）胃癌组织H.pylori-L型阳性组的OPN表达阳性率为70.73%,显著高于H.pylori-L型阴性组OPN表达阳性率42.1%（P〈0.005）。胃癌中H.pylori-L型阳性率与肿瘤的大小、癌细胞的血管侵袭、浸润深度及淋巴结转移具有相关性（P〈0.001～P〈0.05）。（3）胃癌组织中H.pylori-L型阳性率与OPN的阳性表达呈正相关性（r=0.27,P〈0.001）。结论 H.pylori-L型感染增强了胃癌细胞的侵袭和转移能力,其机制可能与胃癌细胞的OPN表达量增加有关。     

胃癌螺旋CT表现与Cath-D蛋白和MMP-9 表达的关系分析

目的:观察分析胃癌螺旋CT表现与组织蛋白酶D(Cath-D)蛋白和基质金属蛋白酶-9(MMP-9)表达的关系。方法:采集自2014年9月至2015年9月在医院经过胃癌手术治疗的54例患者自身胃癌组织标本进行本次研究。再选同期在医院就诊并经过手术治疗的54例胃溃疡患者自身胃黏膜组织进行对照。分别检测MMP-9以及Cath-D蛋白的表达,对所有胃癌患者给予螺旋CT诊断。结果:MMP-9及Cath-D蛋白在胃癌组织内的阳性表达率为74.07%及77.79%,显著高于在正常胃黏膜内的5.56%及9.26%,差异均有统计学意义(P0.05)。MMP-9及Cath-D表达存在明显正相关(r=0.693,P0.05)。胃癌组织内有淋巴结转移及Lauren分型为弥漫型的MMP-9及Cath-D阳性表达率均显著高于无淋巴结转移及Lauren分型为肠型者,差异有统计学意义(P0.05)。根据Logistic回归分析可知,有淋巴结转移以及Lauren分型为弥漫型是胃癌组织内MMP-9及Cath-D表达的螺旋CT表现相关因素。结论:胃癌患者组织内的MMP-9及Cath-D表达比正常胃黏膜组织更高,MMP-9及Cath-D二者的表达呈现正相关,且有淋巴结转移以及Lauren分型为弥漫型是胃癌组织内MMP-9及Cath-D表达的螺旋CT表现相关因素。     

无芽胞厌氧菌、Fas蛋白表达与胃癌相关性的研究

目的探讨无芽胞厌氧菌、Fas蛋白在胃癌及癌旁组织的表达情况及其临床意义。方法无芽胞厌氧菌的检测采用微生物分类鉴定系统中API20A测定方法,Fas蛋白的检测采用免疫组化SP法,分别检测了57例胃癌组织,57例癌旁组织,20例正常胃黏膜组织中微生物的变化及Fas蛋白表达。结果胃癌组织与癌旁组织的无芽胞厌氧菌检出情况不同。胃癌组织检出的主要为革兰阳性无芽胞厌氧菌(优杆菌、丙酸杆菌和消化链球菌等),占检出菌株的6486%(48/74),其中硝酸盐还原试验阳性菌株为5946%(44/74)。癌旁组织检出的主要为革兰阴性无芽胞厌氧菌(类杆菌、梭杆菌、紫单胞菌和韦荣球菌),占检出细菌的5714%(24/42),硝酸盐还原试验阳性菌株为1667%(7/42)。胃癌组织中Fas蛋白表达的阳性率为3509%(20/57),癌旁组织为7018%(40/57),正常胃黏膜为8500%(17/20),胃癌组织与癌旁组织、正常胃黏膜组织Fas蛋白的表达比较,差异均有非常显著性(P<001)。结论胃癌组织与癌旁组织的无芽胞厌氧菌检出情况不同。胃癌组织检出的主要为革兰阳性无芽胞厌氧菌。胃癌组织Fas蛋白水平的表达与癌旁组织、正常胃黏膜组织Fas蛋白的表达比较,差异均有非常显著性(P<001),对阐明胃癌的发生机制、预后和转移均有重要意义。     

Overexpression of ZEB1 associated with metastasis and invasion in patients with gastric carcinoma

The aim of this study was to investigate the expression of ZEB1 in gastric carcinoma, its correlation with the clinicopathology of gastric carcinoma, and the role of ZEB1 in invasion and metastasis in gastric carcinoma. ZEB1 expression was analyzed by immunohistochemistry and Western blot in 45 gastric carcinoma tissue samples that contained the adjacent gastric mucosa. The correlation between ZEB1 expression, the occurrence and development of gastric cancer, and clinical pathology was investigated. ZEB1 expression in the human gastric carcinoma cell line AGS was downregulated by RNA interference, and changes in ZEB1 expression corresponded with changes in the invasive and metastatic ability of AGS cells. Immunohistochemistry revealed that ZEB1 protein expression in gastric carcinoma tissues was significantly higher than in normal gastric mucosa tissues (p < 0.001). A lower degree of differentiation of gastric cancer (p = 0.009), a higher TNM (tumor, node, and metastasis) stage (p = 0.010), and a larger scope of invasion were correlated with higher expression of ZEB1 (p = 0.041, 0.002). However, the expression of ZEB1 in gastric carcinoma tissue was independent of gender, age, and tumor size (p > 0.05). Western blot results also showed that ZEB1 protein expression was significantly higher in gastric carcinoma tissue than in the adjacent normal gastric mucosa tissue (p = 0.008). A lower degree of differentiation of the gastric carcinoma correlated with a higher TNM stage, and a larger scope of invasion correlated with increased ZEB1 expression (p = 0.023). Transfection of ZEB1 siRNA in AGS cells significantly decreased the expression level of ZEB1 protein (p = 0.035). Furthermore, the number of cells that could pass through the Transwell chamber was significantly lower in the transfected group than in the non-transfected control group (p = 0.039), indicating that the suppression of ZEB1 expression could significantly reduce the invasive and metastatic ability of AGS cells (p = 0.005). Concluding, in gastric carcinoma tissue, overexpression of ZEB1 may be related to the occurrence and development as well as invasion and metastasis of gastric carcinoma.     

Up-regulated expression of Ezrin and c-Met proteins are related to the metastasis and prognosis of gastric carcinomas

Recent publications demonstrated that abnormal expression of Ezrin and c-Met proteins were related to carcinogenesis, metastasis and prognosis of various sorts of tumors. In this study we detected the expressions of Ezrin and c-Met proteins in normal gastric mucosa, chronic atrophic gastritis, intestinal metaplasia, dysplasia and gastric carcinoma and analyzed the correlations with metastasis and prognosis of gastric carcinomas. The results demonstrated that both Ezrin and c-Met overexpression were related to the occurrence and progression of gastric carcinoma. Our findings also demonstrated that combined detection of these two tumor-specific biomarkers in gastric carcinomas can provide additional efficacy in predicting the patients' outcomes.     

Expression of FHL1 in gastric cancer tissue and its correlation with the invasion and metastasis of gastric cancer

This study was performed to analyze the expression of four and a half LIM domains 1 (FHL1) in gastric carcinoma tissue and its correlation with the clinicopathological characteristics of gastric cancer. In addition, the role of FHL1 in the invasion and metastasis of gastric cancer cells was investigated to provide an experimental basis for future treatments of gastric cancer. FHL1 mRNA and protein expression in gastric carcinoma and the adjacent normal gastric mucosa tissue were determined using RT-PCR and western blots. Correlations of FHL1 expression with the incidence, progression, and clinicopathological characteristics of gastric cancer were analyzed. Changes in the invasion and metastatic potential of MKN45 human gastric cancer cells were observed after the transient transfection with an eukaryotic expression vector containing full-length FHL1. Expression of FHL1 mRNA in gastric carcinoma tissue was significantly lower than that in the adjacent normal tissue (P < 0.05). FHL1 expression in gastric carcinoma tissue from patients who were positive for lymph node metastasis was significantly lower than those in patients who were negative for lymph node metastasis (P < 0.05). Lower FHL1 expression was correlated with lower degrees of differentiation, higher TNM stages, and greater invasive potential of the gastric cancer (P < 0.05). The FHL1 mRNA and protein expression patterns were similar in gastric cancer. FHL1 protein expression in gastric carcinoma tissue was significantly lower than that in the surrounding normal tissue (P < 0.05). FHL1 protein expression was significantly lower in gastric carcinoma tissue from patients who were positive for lymph node metastasis than that detected in patients with no lymph node metastasis (P < 0.05). Lower FHL1 protein expression was correlated with lower degrees of differentiation, higher TNM stages, and greater invasive potential in gastric cancer (P < 0.05). However, the expression of FHL1 was independent of the patient's gender, age, and tumor size (P > 0.05). Overexpression of FHL1 in the MKN45 human gastric cancer cell line using an eukaryotic expression vector resulted in a significant reduction in the invasiveness and metastatic ability of these cells as determined using the Transwell chamber invasion assay (P < 0.05). The decrease in or loss of FHL1 expression may be related to the incidence, progression, invasiveness, and metastatic potential of gastric cancer.     

在HBV感染的肝癌组织中Fas/FasL表达的作用

目的探讨Fas／FasL（Fas配体）在原发性肝细胞肝癌（HCC）组织中的表达及其与乙肝病毒（HBV）感染的关系。方法用免疫组化S-P法检测21例肝癌组织及其10例癌旁组织Fas／FasL的表达。结果在肝癌和癌旁组织中Fas表达的阳性率分别为52．38％和80．00％（P〈0．05）,FasL分别为66．67％和40．00％（P〈0．05）。在HBsAg阳性和阴性组Fas表达的阳性率分别为50．00％和66．67％（P〉0．05）;FasL分别为61．11％和100％（P〈0．05）。肝癌组织中Fas、FasL表达与性别、年龄、肿瘤大小无关,与癌栓转移呈负相关,FasL与分化程度有关。结论肝癌细胞能下调Fas及上调FasL的表达而使凋亡受阻,HBV感染能抑制肝癌组织FasL的表达,可能是HBV在HCC的发牛及发展讨稃中的致痛机制。     

PGE(2) receptors and synthesis in human gastric mucosa: perturbation in cancer

Recent evidence suggests that prostanoids are an important participant in the pathobiology of gastric adenocarcinoma, but the location and identity of cells in tumor-adjacent gastric mucosa able to synthesize and/or bind specific prostanoids is not clear. Using probes for cyclooxygenase 1 and 2 mRNA and protein as well as for the EP family of PGE(2) receptors, we sought to define the biology of prostanoids in adjacent human gastric mucosa at the site of tumor invasion.In mucosa adjacent to an invasive gastric adenocarcinoma, expression of cyclooxygenase was prominent, with COX 1 primarily in mucosal T lymphocytes surrounding nests of tumor cells. Densitometry showed these tumor-adjacent cells had substantial levels of COX 1 immunoreactive protein (relative intensity, 3.2). Cyclooxygenase 2 was newly expressed among these cells as well, but was limited in number (<25% of cyclooxygenase-positive T lymphocytes) in tumor-adjacent mucosa. Further, CD3(+) mononuclear cells, adjacent to tumor, strongly expressed prostanoid receptor EP(4) (relative intensity, 8.0), but cells with this receptor were not evident in the tumor itself. In contrast, normal gastric mucosa showed a consistent and structured expression of cyclooxygenase and PGE(2) receptor immunoreactive protein among mucosal cells. Cyclooxygenase 1 and PGE(2) receptor EP(4) were expressed on mucosal CD3(+) T lymphocytes in the lumenal (upper) third of gastric mucosa; and prostanoid receptors EP(2), EP(3) and EP(4), on gastric epithelia lining gastric pits. In situ hybridization with COX cDNAs confirmed these findings, and neither COX 2-specific mRNA nor protein was detected in normal gastric tissue. Our studies suggest that synthetic machinery and receptors for PGE(2), prominently expressed by T lymphocytes in gastric mucosa at the boundary of normal mucosa with tumor cells, may play a central role in prostanoid-driven tumorigenesis of this tissue.     

miRNA-105在胃癌组织中的表达情况

目的:分析胃癌组织与癌旁正常胃黏膜组织中miRNA的差异表达情况。方法:收集胃癌组织和其相应癌旁正常胃黏膜组织共33对,经抽提、纯化RNA后,反转录合成荧光分子Hy3的cDNA探针,将其与miRCURYTMLNA Array(v.16.0)(Exiqon公司)芯片进行杂交,应用Axon GenePix 4000B芯片扫描仪来扫描miRNA芯片的荧光强度,GenePix Pro 6.0软件(Axon)把图像转化为数字信号,以差异大于2倍的为标准来确定胃癌黏膜组织中差异表达的miRNA。再用实时荧光定量PCR方法验证芯片结果中表达异常增高的miR-105在33例胃癌组织中的表达情况。结果:miRNA表达谱芯片结果显示:胃癌组织共中有51种miRNA表达异常,其中miR-105、miR-548n、miR-214*、miR-4309等31种miRNA表达上调,miR-31、miR-1275、miR-26b*、miR-744等20种miRNA表达下调;实时荧光定量PCR证实与癌旁正常胃黏膜组织相比,胃癌组织中miR-105表达显著上调(P0.01)。结论:miR-105在胃癌组织的表达明显高于正常胃黏膜组织,可能与胃癌的发生、发展相关。我们的研究为胃癌的发病机制和诊断治疗提供了一个新的研究方向。     

Expression of basic fibroblast growth factor in human gastric carcinomas.

The expression of mRNA for the basic fibroblast growth factor (FGF) gene was examined in seven human gastric carcinoma cell lines and in tissue from 29 gastric carcinomas together with the adjacent normal mucosa. Among the seven gastric carcinoma cell lines, the MKN45 cell line expressed mRNA for the basic FGF gene. Basic FGF protein production was confirmed by flow cytometric analysis and immunohistochemistry. Among the surgical specimens, 16 (55%) of 29 gastric carcinomas showed higher levels of basic FGF mRNA than the normal mucosa. Interestingly, in scirrhous gastric carcinomas characterized by their fibrous stroma and rapid growth, 9 (69%) of 13, samples examined revealed higher levels of basic FGF mRNA than normal mucosa, whereas only 3 (33%) of the 9 well differentiated adenocarcinomas studied produced similar results. Immunohistochemically, basic FGF protein was localized in tumor cells. These results suggest that basic FGF produced by tumor cells may play an important role in producing fibrosis and angiogenesis in gastric carcinomas.     

Expression of basic fibroblast growth factor in human gastric carcinomas

The expression of mRNA for the basic fibroblast growth factor (FGF) gene was examined in seven human gastric carcinoma cell lines and in tissue from 29 gastric carcinomas together with the adjacent normal mucosa. Among the seven gastric carcinoma cell lines, the MKN45 cell line expressed mRNA for the basic FGF gene. Basic FGF protein production was confirmed by flow cytometric analysis and immunohistochemistry. Among the surgical specimens, 16 (55%) of 29 gastric carcinomas showed higher levels of basic FGF mRNA than the normal mucosa. Interestingly, in scirr-hous gastric carcinomas characterized by their fibrous stroma and rapid growth, 9 (69%) of 13, samples examined revealed higher levels of basic FGF mRNA than normal mucosa, whereas only 3 (33%) of the 9 well differentiated adenocarcinomas studied produced similar results. Immunohistochemically, basic FGF protein was localized in tumor cells. These results suggest that basic FGF produced by tumor cells may play an important role in producing fibrosis and angiogenesis in gastric carcinomas.     

PTEN和Fas在骨肉瘤中的表达及其临床意义

目的探讨PTEN和Fas的表达在骨肉瘤发生中的可能作用及其临床意义.方法用SP免疫组化方法检测PTEN和Fas在38例骨肉瘤及20例骨软骨瘤中的表达.结果 PTEN在骨肉瘤中表达阳性率为55.3%,在骨软骨瘤中为90.0%,二者相比差异有非常显著意义(P<0.01).Fas在骨肉瘤中表达阳性率为73.7%,在骨软骨瘤中为95.0%,二者相比差异有显著意义(P<0.05).骨肉瘤中PTEN和Fas的表达呈显著正相关(r=0.39,P<0.01).PTEN的低表达与分化程度无关,与肺转移有关,Fas的低表达与分化程度及肺转移有关.结论 PTEN和Fas与骨肉瘤的发生有关,且呈正相关,联合检测PTEN和Fas的表达将对骨肉瘤的早期诊断,判定恶性程度及预后具有指导意义.