共查询到20条相似文献,搜索用时 31 毫秒
1.
TINA KYNDT ANNELIES HAEGEMAN SABINE VAN GLABEKE INGE MAERTENS BART VAN DROOGENBROECK ISABEL ROLDN‐RUIZ GODELIEVE GHEYSEN 《Molecular ecology resources》2005,5(3):590-592
Nine polymorphic microsatellite loci were identified in the tropical plant Vasconcellea ×heilbornii and used to estimate allelic diversity in two populations of southern Ecuador. Allelic richness ranged from two to five alleles, observed heterozygosity ranged from 0.150 to 0.947 and expected heterozygosity ranged from 0.186 to 0.701. Most of these markers also amplified microsatellite loci in two other Vasconcellea species (Vasconcellea stipulata and Vasconcellea cundinamarcensis). Hence, these markers will be useful for population genetic analysis and the evaluation of genetic diversity and gene flow in these species. 相似文献
2.
Xiuli Wang Teng Feng Lei Yang Chengcheng Liu Xiangying Meng Xuemei Qiu 《Conservation Genetics》2009,10(3):729-731
Sea urchin is an important model organism for evolutionary biology, embryology, and gene regulation study. We developed and
evaluated simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) of Strongylocentrotus
purpuratus and Hemicentrotus pulcherrimus. Characteristics of nine EST-SSR loci were investigated using 41 Hemicentrotus pulcherrimus individuals. The number of alleles per locus ranged from two to five. The observed heterozygosity ranged from 0.122 to 0.7805, while the
expected heterozygosity ranged from 0.4472 to 0.7696. These loci and markers will be useful for population genetics and systemic
evolution among species of sea urchin. 相似文献
3.
L. REX MCALILEY RAYMOND E. WILLIS MICHAEL R. J. FORSTNER TRINA GUERRA LLEWELLYN D. DENSMORE 《Molecular ecology resources》2006,6(3):759-761
We characterize eight polymorphic microsatellite loci for the San Esteban chuckwalla, Sauromalus varius, which are being used as markers in a study of the genetic results from a decade of captive breeding in this endangered species. Observed heterozygosity estimates for S. varius ranged from 0.00 to 0.30, whereas expected heterozygosity estimates ranged from 0.06 to 0.58. 相似文献
4.
Xiuli Wang Xiaoli Guo Yingming Zhang Xiangying Meng Xuemei Qiu Shaozhen Liu Tianxiao Zhang 《Conservation Genetics》2009,10(5):1455-1457
In our study, we evaluated simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) of Fenneropenaeus chinensis. Characteristics of 11 EST-SSR loci were investigated using 34 F. chinensis individuals. The number of alleles per locus ranged from 4 to 9. The observed heterozygosity ranged from 0.118 to 0.826,
while the expected heterozygosity ranged from 0.623 to 0.847. Two loci (T2 and T12) conformed to the Hardy–Weinberg equilibrium.
There was no LD observed between all pairs of EST-SSRs loci. The PIC values of 11 microsatellite loci were higher than 0.5.
These loci and markers will be useful for population genetics and systemic evolution of F. chinensis. 相似文献
5.
Small yellow croaker (Larimichthys polyactis) is an important economic species of marine fishery. We developed and evaluated simple sequence repeat (SSR) markers from
expressed sequence tags (ESTs) of Pseudosciaena crocea, Paralichthys olivaceus and Psetta maxima. Characteristics of nine EST–SSR loci were investigated using 46 L. polyactis individuals. The number of alleles per locus ranged from 2 to 6. The observed heterozygosity ranged from 0.0652 to 0.7391,
while the expected heterozygosity ranged from 0.0638 to 0.7754. Seven loci departed from Hardy–Weinberg equilibrium (P < 0.01) significantly. These loci and markers will be useful for population genetics and systemic evolution among species
of small yellow croaker. 相似文献
6.
XIN JIANG MEIJIE LIAO YONGJIAN LIU TIANXIANG GAO GUANPIN YANG 《Molecular ecology resources》2007,7(3):492-494
Japanese sea bass (Laterolabrax japonicus) inhabits the coast of East Asia and is cage‐cultured currently in China as well. Twenty‐two microsatellite DNA markers were developed and used to type 35 individuals collected along the Chinese coast. The number of alleles per locus ranged from three to 23. The expected heterozygosity ranged from 0.111 to 0.938, while the observed heterozygosity ranged from 0.114 to 0.914. All 22 loci are neutral and independent of each other; two deviated significantly from Hardy–Weinberg equilibrium. These microsatellite DNA markers are moderately informative, which will certainly facilitate the management and exploitation of the genetic resource of L. japonicus. 相似文献
7.
The orchid genus Vanda includes more than 70 monopodial species and numerous hybrids. The aim of this study was to develop microsatellite markers for this horticultural valuable genus. Thirteen polymorphic microsatellite markers were isolated from the variety Vanda Miss Joaquim and were characterized in four Vanda species, 11 Vanda hybrids, and one Aranda intergenus hybrid. Populations from three species were also analysed. Number of alleles ranged from two to 19. For the V. sumatrana population, the expected heterozygosity ranged from 0 to 0.76 (mean 0.31) and the observed heterozygosity ranged from 0 to 1 (mean 0.36). All the varieties tested were distinct from one another (similarity index < 0.8). These microsatellites could be used for studying genetic diversity and population structure of wild populations within the orchid genus Vanda, as well as for distinguishing cultured Vanda varieties. 相似文献
8.
Oyster (Crassostrea gigas) is widely distributed in coastal areas of world. We developed and evaluated simple sequence repeat (SSR) markers from expressed
sequence tags (ESTs) of C. gigas and to amplify EST-SSR in C. gigas. Characteristics of 11 EST-SSR loci were investigated using 45 C. gigas individuals. The number of alleles per locus ranged from two to thirteen. The observed heterozygosity (H
o) ranged from 0.0889 to 0.7333 and the expected heterozygosity (H
e) ranged from 0.0859 to 0.8981. Because of their high level of polymorphism, our 11 single-locus EST-SSR markers will be valuable
tools for research on mating system, population genetics and systemic evolution of oyster in the future. 相似文献
9.
Zorisadday Gonzalez David A. Ray L. Rex McAliley Matthew J. Gray Celine Perchellet Loren M. Smith Llewellyn D. Densmore 《Molecular ecology resources》2004,4(1):9-10
We characterize five polymorphic microsatellite loci for the Great Plains toad, Bufo cognatus, which are being used as markers in a study of the genetics and structure of toad populations inhabiting playa lakes on the Llano Estacado plateau of Texas. Observed heterozygosity (HO) estimates for the toads ranged from 0.56 to 0.90, and expected heterozygosity (HE) estimates ranged from 0.85 to 0.95. 相似文献
10.
We developed 22 microsatellite loci for boreal owls (Aegolius funereus). We genotyped 275 unrelated boreal owls (Aegolius f. richardsoni) and 36 unrelated Tengmalm's owls (Aegolius f. funereus) using seven loci that were polymorphic and did not have detectable null alleles. Among North American and Scandinavian boreal owls, respectively, allelic diversity ranged from three to 11 alleles and from one to 11 alleles, observed heterozygosity ranged from 0.31 to 0.80 and from 0.00 to 0.81, and expected heterozygosity ranged from 0.28 to 0.81 and from 0.00 to 0.81. These markers appeared to amplify DNA in six other Strigidae species. 相似文献
11.
GUANGXIANG TONG YOUYI KUANG JIASHENG YIN LIQUN LIANG XIAOWEN SUN 《Molecular ecology resources》2006,6(4):1099-1101
Hucho taimen (Pallas) is an endangered species in China. To evaluate the species’ genetic diversity and population genetic structure, we isolated six polymorphic microsatellite markers from its genomic libraries by (ACA)16 enriched, and characterized using 61 wild individuals. The number of alleles per locus ranged from two to seven, expected heterozygosity ranged from 0.3200 to 0.7410, polymorphism information content ranged from 0.3047 to 0.6896. These markers will be useful for the genetic variation assessment of taimen. 相似文献
12.
Ayako Sawa Shingo Kaneko Yuji Isagi Shigeru Mariko Takashi Masaki 《Conservation Genetics》2010,11(3):1167-1169
Simple sequence repeat (SSR) markers were developed for Prunus verecunda and Prunus
grayana to help understand the seed dispersal pattern of each species. We isolated and characterized nine microsatellite loci (four
from P. verecunda and five from P. grayana). In P. verecunda, the number of alleles detected and the expected heterozygosity of five loci ranged from 11 to 24 and 0.59 to 0.92, respectively.
In P.
grayana, the number of alleles detected and the expected heterozygosity of five loci ranged from 4 to 14 and 0.62 to 0.86, respectively.
These results show that the markers described here will be useful in studying the seed dispersal pattern of Prunus species. 相似文献
13.
S. NANAMI S. IKEDA N. TANI S. TAN B. DIWAY K. HARADA Y. TSUMURA A. ITOH T. YAMAKURA 《Molecular ecology resources》2007,7(4):623-625
Eight polymorphic microsatellite markers were developed in Dryobalanops aromatica, an emergent tree in tropical rain forests in Southeast Asia, using an enriched library method. For the assessment of microsatellite variation, 36 individuals from a natural population were analysed. The number of alleles per locus ranged from three to 16, with observed heterozygosity of 0.056–0.833 and expected heterozygosity of 0.054–0.882. These microsatellite markers will be useful for studies of population genetics, reproductive ecology and regeneration dynamics of D. aromatica. 相似文献
14.
Hye Suck An Jung Youn Park Mi-Jung Kim Eun Young Lee Kyung Kil Kim 《Conservation Genetics》2009,10(6):1969-1972
Here we report development and characterization of 14 polymorphic microsatellite loci from Sebastes schlegel. Polymorphism at these loci revealed from 3 to 23 alleles. The observed heterozygosity ranged from 0.34 to 1.00, and the
expected heterozygosity ranged from 0.31 to 0.95. No linkage disequibrium was found. Two loci were significantly deviated
from HWE (P < 0.01). The 14 loci were also surveyed in four other Sebastes species and 12 loci successfully amplified, where allelic diversity ranged from highly polymorphic to monomorphic. These
results demonstrate these microsatellite markers can be used for the study of intra- and inter-specific genetic diversity. 相似文献
15.
Development and characterization of EST‐SSR markers for the genus Rhododendron section Brachycalyx (Ericaceae)
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Watanabe Yoichi Shota Sakaguchi Saneyoshi Ueno Nobuhiro Tomaru Koichi Uehara 《Plant Species Biology》2017,32(4):455-459
Simple sequence repeat (SSR) markers were developed from expressed sequence tags (ESTs) for Rhododendron section Brachycalyx in order to elucidate its evolutionary processes and reproductive ecology. Nineteen polymorphic EST‐SSR markers were developed from EST libraries of R. amagianum and R. hyugaense. Polymorphisms for these markers were assessed using four species of section Brachycalyx. The number of alleles ranged from 1 to 14, and the observed and expected heterozygosity ranged from 0.000 to 0.931 and 0.000 to 0.904, respectively. The EST‐SSR markers developed in this study will be useful for elucidating population genetic structure and breeding systems in section Brachycalyx. 相似文献
16.
Patinopecten yessoensis is a kind of cold water shellfish, and is an important economic species in China. In our study, we developed and evaluated
simple sequence repeat (SSR) markers of P. yessoensis. Characteristics of 11 EST-SSR loci were investigated using 40 P. yessoensis individuals. The number of alleles per locus ranged from two to five. The observed heterozygosity ranged from 0.1026 to 0.9487, while
the expected heterozygosity ranged from 0.1865 to 0.7433. All loci except P4 departed from Hardy–Weinberg equilibrium (P < 0.05) significantly. There was no LD observed between all pairs of EST-SSRs loci. These loci and markers will be useful
for population genetics and systemic evolution of scallop. 相似文献
17.
Anna Barinova Elena Yadrenkina Masamichi Nakajima Nobuhiko Taniguchi 《Molecular ecology resources》2004,4(1):86-88
The first five microsatellite markers for the ide, Leuciscus idus, and four microsatellite markers for the Siberian roach, Rutilus rutilus, were designed. Cross‐amplification of ide markers was examined in Siberian roach and vice versa. The number of alleles per locus ranged from three to 13 in ide and from two to eight in roach. The expected heterozygosity ranged from 0.313 to 0.909 in ide and from 0.119 to 0.775 in roach. These markers could be used to evaluate the genetic population structure of these species and other fish from the Cyprinidae family. 相似文献
18.
R. John Nelson Glenn Cooper Trent Garner Pamela Schnupf 《Molecular ecology resources》2002,2(3):233-235
Five polymorphic markers were isolated from a Parastichopus californicus genomic DNA library. In addition, a method was developed to extract DNA from both muscle and skin tissue. The number of alleles at these loci ranged from three to 14. Observed heterozygosity ranged from 0.2 to 0.9. These markers, the first of their type for a sea cucumber species, will prove useful for exploration of the population structure of P. californicus. 相似文献
19.
We have isolated nine polymorphic microsatellite markers for the bay pipefish Syngnathus leptorhynchus from genomic libraries enriched for (AAGG)n repetitive elements. The number of alleles ranged from two to 15 per locus with the observed heterozygosity ranging from 0.09 to 1.00. These markers will be useful for analyses of questions concerning population genetic structure. 相似文献
20.
Sixteen microsatellite loci were identified and characterized for American shad (Alosa sapidissima). The number of alleles per locus observed ranged from eight to 32 and averaged 15.4 alleles. Average observed heterozygosity was 81.1%. The markers were screened using four other species from the family Clupeidae. Amplification success among Alosa species was 79.2% with 81.6% polymorphism among those markers that amplified successfully. Amplification success was poor in Dorosoma (31.3%). Due to allelic diversity and estimates of heterozygosity, these markers can be useful in A. sapidissima for population level analyses, parentage assignment and broodstock management. 相似文献