共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
Monica C. Roberts Christophe C. Hitte Julie A. Hendrickson Daniel E. Hoffmann Gail H. Flickinger Mark S. Rutherford Richard S. Guyon Francis S. Galibert James R. Mickelson 《Mammalian genome》2003,14(3):203-213
Abstract
Maps of the canine genome are now developing rapidly. Most of the markers on the current integrated canine radiation hybrid/genetic
linkage/cytogenetic map are highly polymorphic microsatellite (type II) markers that are very useful for mapping disease loci.
However, there is still an urgent need for the mapping of gene-based (type I) markers that are required for comparative mapping,
as well as identifying candidate genes for disease loci that have been genetically mapped. We constructed an adult brain cDNA
library as a resource to increase the number of gene-based markers on the canine genome map. Eighty-one percent of the 2700
sequenced expressed sequence tags (ESTs) represented unique sequences. The canine brain ESTs were compared with sequences
in public databases to identify putative canine orthologs of human genes. One hundred nine of the canine ESTs were mapped
on the latest canine radiation hybrid (RH) panel to determine the location of the respective canine gene. The addition of
these new gene-based markers revealed three conserved segments (CS) between human and canine genomes previously detected by
fluorescence in situ hybridization (FISH), but not by RH mapping. In addition, five new CS between dog and human were identified that had not
been detected previously by RH mapping or FISH. This work has increased the number of gene-based markers on the canine RH
map by approximately 30% and indicates the benefit to be gained by increasing the gene content of the current canine comparative
map. 相似文献
4.
5.
To obtain an initial overview of gene diversity and expression pattern in porcine thymus, 11,712 ESTs (Expressed Sequence Tags) from 100-day-old porcine thymus (FTY) were sequenced and 7,071 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 959 contigs and 3,074 singlets were obtained. Blast search showed that 806 contigs and 1,669 singlets (totally 5,442 ESTs) had homologues in GenBank and 1,629 ESTs were novel. According to the Gene Ontology classification, 36.99% ESTs were cataloged into the gene expression group, indicating that although the functional gene (18.78% in defense group) of thymus is expressed in a certain degree, the 100-day-old porcine thymus still exists in a developmental stage. Comparative analysis showed that the gene expression pattern of the 100-day-old porcine thymus is similar to that of the human infant thymus. 相似文献
6.
7.
8.
Dvorak CM Flickinger GH Hendrickson JA Fahrenkrug SC Murtaugh MP Rutherford MS 《Animal genetics》2006,37(1):62-65
To determine the chromosomal locations for genes expressed in porcine Peyer's patches, polymerase chain reaction-based mapping of expressed sequence tags (ESTs) isolated from a porcine Peyer's patch-specific cDNA library was performed across a 6500-rad swine radiation hybrid panel. A total of 116 ESTs were mapped with LOD scores >6.0, and another 11 ESTs had LOD scores between 5.0 and 6.0. Of these 127 ESTs, 63% matched known genes (相似文献
9.
G. Bain F.C. Mansergh M.A. Wride J.E. Hance A. Isogawa S.L. Rancourt W.J. Ray Y. Yoshimura T. Tsuzuki D.I. Gottlieb D.E. Rancourt 《Functional & integrative genomics》2000,1(2):127-139
We have used a method for synchronously differentiating murine embryonic stem (ES) cells into functional neurons and glia
in culture. Using subtractive hybridization we isolated approximately 1200 cDNA clones from ES cell cultures at the neural
precursor stage of neural differentiation. Pilot studies indicated that this library is a good source of novel neuro-embryonic
cDNA clones. We therefore screened the entire library by single-pass sequencing. Characterization of 604 non-redundant cDNA
clones by BLAST revealed 96 novel expressed sequence tags (ESTs) and an additional 197 matching uncharacterized ESTs or genomic
clones derived from genome sequencing projects. With the exception of a handful of genes, whose functions are still unclear,
most of the 311 known genes identified in this screen are expressed in embryonic development and/or the nervous system. At
least 80 of these genes are implicated in disorders of differentiation, neural development and/or neural function. This study
provides an initial snapshot of gene expression during early neural differentiation of ES cell cultures. Given the recent
identification of human ES cells, further characterization of these novel and uncharacterized ESTs has the potential to identify
genes that may be important in nervous system development, physiology and disease.
Electronic Publication 相似文献
10.
11.
12.
13.
14.
Vasmatzis G Klee EW Kube DM Therneau TM Kosari F 《Bioinformatics (Oxford, England)》2007,23(11):1348-1355
We describe a method to identify candidate cancer biomarkers by analyzing numeric approximations of tissue specificity of human genes. These approximations were calculated by analyzing predicted tissue expression distributions of genes derived from mapping expressed sequence tags (ESTs) to the human genome sequence using a binary indexing algorithm. Tissue-specificity values facilitated high-throughput analysis of the human genes and enabled the identification of genes highly specific to different tissues. Tissue expression distributions for several genes were compared to estimates obtained from other public gene expression datasets and experimentally validated using quantitative RT-PCR on RNA isolated from several human tissues. Our results demonstrate that most human genes ( approximately 98%) are expressed in many tissues (low specificity), and only a small number of genes possess very specific tissue expression profiles. These genes comprise a rich dataset from which novel therapeutic targets and novel diagnostic serum biomarkers may be selected. 相似文献
15.
Piquemal D Commes T Manchon L Lejeune M Ferraz C Pugnère D Demaille J Elalouf JM Marti J 《Genomics》2002,80(3):361-371
16.
Shi XW Fitzsimmons CJ Genêt C Prather R Whitworth K Green JA Tuggle CK 《Animal genetics》2001,32(4):205-209
A comparative study of human chromosome 17 (HSA17) and pig chromosome 12 (SSC12) was conducted using both somatic cell hybrid panel (SCHP) and radiation hybrid (RH) panel analysis. Sequences from an expressed sequence tag (EST) project in pig reproduction were examined and six genes and ESTs originally believed to map to HSA17 were selected for this study. The genes/ESTs were TATA box binding protein-associated factor (TAF2N/RBP56), alpha-2-plasmin inhibitor (SERPINF2/PLI), H3 histone family 3B (H3F3B), aminopeptidase puromycin sensitive (NPEPPS), an expressed sequence tag (ESTMI015) and P311 protein (P311). The SCHP analysis mapped five genes/ESTs (TAF2N, H3F3B, SERPINF2, NPEPPS and ESTMI015) to SSC12q11-q15 and SSC12p11-p15 with 100% concordance, and assigned P311 to SSC2 (1/2q24)-q29 with 100% concordance. Radiation hybrid analysis of all six genes confirmed the SCHP mapping results, with average retention frequency of 25%. Recent human sequence data demonstrated that P311 is actually located on HSA5q. As HSA5q and SSC2q show conserved syntenic regions predicted from bi-directional painting, our P311 mapping data is consistent with these results. An expanded comparative SSC12 RH map integrating the five new type I markers and 23 previously mapped loci was established using a LOD score threshold of 4.8. The gene order of the five genes/ESTs on the SSC12 framework RH map (H3F3B-ESTMI015-NPEPPS-TAF2N-SERPINF2) is identical to the HSA17 GB4 map but with inversion of the map as conventionally drawn. 相似文献
17.
病毒感染草鱼胸腺的EST分析和免疫相关基因的鉴定 总被引:1,自引:0,他引:1
以感染草鱼(Ctenopharyngodon idellus)出血病病毒(GCHV)的草鱼胸腺为材料,构建了草鱼胸腺的SMARTcDNA文库.筛选文库获得到1933条有效EST序列.BLASTX分析显示,583条序列在公共数据库中能找到同源基因(E-value≤1.00E 10-3,Identities≥30%),另外1350条序列则找不到显著同源性.已知基因按具体功能可划分为6类,大部分与细胞内的各种生理过程、细胞结构以及免疫防御相关.研究结果从分子水平上表明鱼类的胸腺在机体感染病毒的免疫反应中发挥重要作用,同时也表明胸腺组织在病毒感染后可能表达很多目前还不清楚功能的新基因. 相似文献
18.
19.