共查询到20条相似文献,搜索用时 15 毫秒
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Yaju Wang Shilpa Rani Shankar Devaki Kher Belinda Mei Tze Ling Reshma Taneja 《The Journal of biological chemistry》2013,288(24):17654-17662
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Daxx mediates the small ubiquitin-like modifier-dependent transcriptional repression of Smad4 总被引:4,自引:0,他引:4
Chang CC Lin DY Fang HI Chen RH Shih HM 《The Journal of biological chemistry》2005,280(11):10164-10173
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Mascle XH Germain-Desprez D Huynh P Estephan P Aubry M 《The Journal of biological chemistry》2007,282(14):10190-10202
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Nishida T Terashima M Fukami K 《Biochemical and biophysical research communications》2006,345(4):1536-1546
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Knuesel M Cheung HT Hamady M Barthel KK Liu X 《Molecular & cellular proteomics : MCP》2005,4(10):1626-1636
Post-translational modification by small ubiquitin-like modifier 1 (SUMO-1) is a highly conserved process from yeast to humans and plays important regulatory roles in many cellular processes. Sumoylation occurs at certain internal lysine residues of target proteins via an isopeptide bond linkage. Unlike ubiquitin whose carboxyl-terminal sequence is RGG, the tripeptide at the carboxyl terminus of SUMO is TGG. The presence of the arginine residue at the carboxyl terminus of ubiquitin allows tryptic digestion of ubiquitin conjugates to yield a signature peptide containing a diglycine remnant attached to the target lysine residue and rapid identification of the ubiquitination site by mass spectrometry. The absence of lysine or arginine residues in the carboxyl terminus of mammalian SUMO makes it difficult to apply this approach to mapping sumoylation sites. We performed Arg scanning mutagenesis by systematically substituting amino acid residues surrounding the diglycine motif and found that a SUMO variant terminated with RGG can be conjugated efficiently to its target protein under normal sumoylation conditions. We developed a Programmed Data Acquisition (PDA) mass spectrometric approach to map target sumoylation sites using this SUMO variant. A web-based computational program designed for efficient identification of the modified peptides is described. 相似文献
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