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1.
There is some controversy concerning the presence of steryl glycosides and acylated steryl glycosides in eucaryotic algae. These two classes of sterol compounds were investigated in species belonging to the three major groups of eucaryotic algae: green algae (Ulva gigantea, Cladophora rupestris), brown algae (Fucus vesiculosus, Ascophyllum nodosum), and red algae (Rhodymenia palmata, Porphyridium sp.). All these algae contain both steryl glycosides and acylated steryl glycosides. The sterol components of these compounds vary according to the alga but they are always the same as the free sterols of the alga in question. The most common sugar moiety is glucose. In the acylated steryl glycosides, the fatty acid is mainly palmitic acid. The percentage of these compounds (as a percentage of the total sterol content) is often low. 相似文献
2.
In 3- and 14-day-old seedlings and in the leaves of Calendula officinalis the following sterols were identified: cholestanol, campestanol, stigmastanol, cholest-7-en-3-β-ol, 24-methylcholest-7-en-3β-ol, stigmast-7-en-3β-ol, cholesterol, campesterol, sitosterol, 24-methylcholesta-5,22-dien-3β-ol, 24-methylenecholesterol, stigmasterol and clerosterol. Sitosterol was predominant in young and stigmasterol in old tissues. Young tissues contained relatively more campesterol but in old tissues a C28Δ5,22 diene was present suggesting transformation of campesterol to its Δ5,22 analog, similar to that of sitosterol to stigmasterol. All the identified sterols were present as free compounds and also in the steryl esters, glucosides, acylated glucosides and water-soluble complexes. The variations in the amounts of these fractions in the embryo axes and cotyledons of 3- and 14-day-old seedlings and the distribution of individual sterols among the fractions are discussed. 相似文献
3.
With 14CO2, d-glucose-[U-14C] and dl-mevalonate-[4R-4-3H1] used as precursors, a study was made of the labelling dynamics of the steryl glucosides (SG) and steryl acylglucosides (ASG) in Sinapis alba seedlings. The radioactivity of the sterol and sugar moieties, as well as of the fatty acid moieties in the case of ASG, was analysed separately. The course of incorporation of 14C from 14 CO2 and glucose-[U-14C] into the sugar part of SG and ASG indicated that about of the whole pool of the newly synthesized sterol glycosides of both types underwent rapid deglucosylation. Likewise, fatty acids in the ASG pool were rapidly exchanged. The present results point to a high metabolic activity of the sterol glycoside derivatives in plant cells. 相似文献
4.
Riccardo Cerri Francesco de Simone Antonio Dini Eliseo Ramundo 《Biochemical Systematics and Ecology》1985,13(1):11-13
Free and esterified sterol levels in seeds of five cultivars of Vicia faba were determined. Sitosterol was the most abundant free sterol, followed by stigmasterol and campesterol. Cholesterol could not be detected. Esters were generally present in greater quantities than the free form of the sterols. The fatty acid content of the plants is also reported. 相似文献
5.
Abstract Complete separation of the steryl and wax esters in the subarctic moss Dicranum elongatum was achieved on MgO thin-layer plates without any notable alteration of the acyl and alkyl moieties of the esters. Gas chromatography-mass spectrometry of the hydrolyzed fraction showed that the sterols (campesterol, stigmasterol, sitosterol, cycloartenol, 24-methylene cycloartanol and an unidentified sterol) were primarily esterified with unsaturated fatty acids 18:2 ω 6, 18:3 ω 3 and 20:4 ω 6. In contrast, the wax alcohols (l-octadecanol, phytol and geranylgeraniol) were mainly esterified with saturated fatty acids with 16:0, 18:0 and 20:0 as major components. No great differences were found in the fatty acid pattern of the steryl esters between different portions of the shoot. Slight differences, however, were found in the proportions of ω 3 and ω 6 fatty acids. In the wax esters a clear decrease was found in the proportions of 18:0 and 20:0 acids with increased shoot age accompanied by a slight increase in the proportions of 14:0, 20:4 ω 6 and phytenic acid. 相似文献
6.
Usuki S Ariga T Dasgupta S Kasama T Morikawa K Nonaka S Okuhara Y Kise M Yu RK 《Journal of lipid research》2008,49(10):2188-2196
Previous studies from our laboratory indicated that pre-germinated brown rice (PR) contained certain unknown bioactive lipids that activated two enzymes related to diabetes: Na+/K+ATPase and homocysteine-thiolactonase. In this paper, we report on the isolation and structural characterization of the activator lipids from PR bran as acylated steryl glucosides (ASGs). The activator lipid was isolated by silica gel column chromatography, and its chemical structure was determined by NMR, GC-MS, and tandem mass spectrometry. We demonstrated that the bioactive component consists of a mixture of acylated steryl beta-glucosides. Delta8-cholesterol and 2-hydroxyl stearic acid were identified as constituents of ASGs. The steryl glucosides (SGs) subsequent to alkaline hydrolysis lost this enzyme activator activity. Soybean-derived ASGs were not active. This activity may be quite peculiar to PR-derived ASGs. Our findings suggest that the molecular species of ASG may play an important contributing role in the anti-diabetic properties of a PR diet. 相似文献
7.
Lyons GH Genc Y Stangoulis JC Palmer LT Graham RD 《Biological trace element research》2005,103(2):155-168
Selenium (Se) is an essential micronutrient for animals and humans, and wheat is a major dietary source of this element. It
is improtant that postharvest processing losses of grain Se are minimized. This study, using grain dissection, milling with
a Quadrumat mill, and baking and toasting studies, investigated the distribution of Se and other mineral nutrients in wheat
grain and the effect of postharvest processing on their retention. The dissection study, although showing Se concentration
to be highest in the embryo, confirmed (along with the milling study) previous findings that Se (which occurs mostly as selenomethionine
in wheat grain) and S are more evenly distributed throughout the grain when compared to other mineral nutrients, and hence,
lower proportions are removed in the milling residue. Postmilling processing did not affect Se concentration or content of
wheat products in this study.
No genotypic variability was observed for grain distribution of Se in the dissection and milling studies, in contrast to Cu,
Fe., Mn, and Zn. This variability could be exploited in breeding for higher proportions of these nutrients in the endosperm
to make white flour more nutritious. Further research could include grain dissection and milling studies using larger numbers
of cultivars that have been grown together and a flour, extraction rate of around 70% 相似文献
8.
Sharifa Alkandari Mohammad E. Bhatti Ahmed Aldughpassi Fatima Al-Hassawi Mohammad Al-Foudari Jiwan S. Sidhu 《Saudi Journal of Biological Sciences》2021,28(6):3602-3606
Wheat grain is a rich source of phosphorus which is present mostly as phytic acid and is distributed mainly in the bran and germ fractions. Phytic acid has now been recognized as an important phytochemical having antioxidant properties. This study deals with the determination of total as well as phytic phosphorus contents of psyllium (PS), course (CB) and fine wheat bran (FB) enriched pan bread and Arabic flat bread. The concentration of phytic acid in CB, FB, wheat germ, wholegrain wheat flour (WGF), white wheat flour (WWF), and psyllium were found to be 8.86 mg/g, 8.52 mg/g, 6.05 mg/g, 1.74 mg/g, 0.46 mg/g and 0.02 mg/g, respectively. Most of the phosphorus existed as phytic phosphorus (74.7–90.8%) in FB, CB, germ, and WGF as compared to only 42.6% in WWF. The level of phytic phosphorus in pan bread containing 10% CB, 20% FB (both containing with 5% PS) was found to be 0.63 mg/g and 1.53 mg/g respectively, as compared to only 0.34 mg/g in WWF pan bread, and 0.90 mg/g in WGF pan bread. The phytic phosphorus content in Arabic bread made with WGF and 3% psyllium was 1.32 mg/g as compared to only 0.48 mg/g in WWF Arabic flat bread. The results obtained indicate that the level of phytic phosphorus significantly increased in bread formulations containing CB, FB, and WGF, but no change with psyllium addition was observed. Adding these wheat mill fractions, and psyllium will enable bakeries not only to produce fiber-enriched pan bread and Arabic bread but would also benefit consumers to increase their dietary fiber intakes, and health-promoting phytochemicals coming from wheat bran and germ fractions. 相似文献
9.
The ste1 gene encoding a steryl esterase was isolated from the thermophilic fungus Melanocarpus albomyces. The gene has one intron, and it encodes a protein consisting of 576 amino acids. The deduced amino acid sequence of the steryl esterase was shown to be related to lipases and other esterases such as carboxylesterases. Formation of mature protein requires post-translational removal of a putative 18-amino-acid signal sequence and a 13-residue propeptide at the N-terminus. The intronless version of the Melanocarpus albomyces ste1 gene was expressed in Pichia pastoris under the inducible AOX1 promoter. The production level was low, and a large proportion of the total activity yield was found to be present intracellularly. However, the fact that steryl esterase activity was produced by P. pastoris cells carrying the expression cassette confirmed that the correct gene had been cloned. The ste1 gene was subsequently expressed in T. reesei under the inducible cbh1 promoter, and a clearly higher production level was obtained. About 60% of the total activity was bound to the fungal mycelium or to solid components of the culture medium, or existed as aggregates. Triton X-100 was successfully used to recover this activity. The heterologous production system in T. reesei provides a means of producing M. albomyces steryl esterase STE1 reliably in large scale for future studies. 相似文献
10.
Hisae Haruta Hideo Yagi Takashi Iwata Saburo Tamura 《Bioscience, biotechnology, and biochemistry》2013,77(4):877-879
The genetic background of the protein-leaky mutant of Escherichia coli (PE4LA) was examined. This mutant preferentially excretes periplasmic proteins and outer membrane components. The results obtained from various crosses indicated that the mutation responsible for protein leakage was located near the purE gene. The amount of protein excreted decreased to about half whenever PE4LA was converted from Pur? to Pur+ either by transduction or spontaneous reversion. When purine or pyrimidine auxotrophs were obtained from purE+ revertant of PE4LA, only auxotrophs whose blockages were located at a step identical or close to that governed by purE gene excreted proteins to the same extent as PE4LA. Excess adenine in the culture medium repressed exprotein accumulation by PE4LA. These results suggest that purE is involved in some way with protein excretion, and perhaps the accumulating metabolic intermediate(s) causes protein excretion due to a defect in purE enzyme. 相似文献
11.
Endogenous abscisic acid and wheat germ agglutinin content in wheat grains during development 总被引:1,自引:0,他引:1
Peter-Christian Morris 《Physiologia plantarum》1989,77(4):507-511
Abscisic acid (ABA) and wheat germ agglutinin content of immature wheat grains and embryos was determined by immunoassay throughout the development of a field-grown wheat crop ( Triticum aestivum cv. Timmo). Wheat germ agglutinin accumulation in the embryo was not preceded by an increase in endogenous abscisic acid amount or concentration in either embryos or grains. At a later stage in development the endogenous concentration of abscisic acid in both embryos and grains was found to be two orders of magnitude lower than the endogenous levels required to inhibit precocious germination and promote wheat germ agglutinin accumulation in excised embryos cultured in vitro. These findings are discussed in the context of the control of embryo development in vivo by both ABA and the water status of the grain and embryo. 相似文献
12.
Steryl glucoside (SG) and acylated steryl glucoside (ASG) synthesis was investigated in amyloplast membranes from young, intermediate and mature potato tubers. The synthesis ratio SG/ASG was lowest in young tubers (3:2) and highest in mature tubers (6:1).About a 3-fold stimulation of [14C] glucose incorporation into a mixture of SG was observed in amyloplast membranes from mature tubers in the presence of β-sitosterol, while radioactivity incorporation in young tubers was unaffected, thus indicating that different availabilities of endogenous acceptors occur in the membranes.The enzymes involved in sterol modification exhibit a different behavior towards Triton X-100, depending on the developmental stage of the tubers. Low concentrations of the detergent (0.045%) are required to stimulate the enzymes present in young tuber membranes (2-fold). On the other,hand, 0.15% of Triton increased the enzymatic activity in mature tubers 5-fold. These results, together with those obtained after studies of pH dependence, could be related to the lipidic structure of the vesicles formed at different developmental stages of the tubers.It is concluded that the major changes in the enzymatic activities occur as a consequence of the sterol acceptors and acyl donor content during potato tuber growth. 相似文献
13.
小麦花后弱光引起籽粒淀粉的粒度分布及组分含量的变化 总被引:10,自引:0,他引:10
在籽粒灌浆阶段(花后1~30 d)对小麦进行光强为自然光照45%的弱光处理,研究了小麦籽粒淀粉粒度分布和组分含量的变化.结果表明,小麦籽粒淀粉粒体积分布呈双峰曲线,峰值分别在5.1~6.1 μm和20.7~24.9 μm,两峰值间的低谷出现在9.9 μm左右.表面积分布和数目分布分别表现为双峰和单峰曲线.小麦花后弱光显著降低2.8~9.9 μm淀粉粒体积百分比,增加22.8~42.8 μm淀粉粒体积百分比.同时花后弱光显著降低<0.8 μm和2.8~9.8 μm淀粉粒表面积百分比,增加0.8~2.8 μm和>9.9 μm淀粉粒表面积百分比.可见灌浆期弱光显著降低籽粒B型(<9.9 μm)淀粉粒体积和表面积百分比,而A型(>9.9 μm)淀粉粒比例相对增加.与A型淀粉粒相比,B型淀粉粒对弱光的反映更敏感.小麦弱光处理籽粒淀粉及其组分含量显著低于对照,但其直/支比较对照高.相关分析表明,籽粒直/支比与2.8~9.9 μm淀粉粒体积百分比呈显著负相关,而与22.8~42.8 μm淀粉粒体积百分比呈显著正相关.花后不同阶段弱光显著增加A型淀粉粒体积百分比、降低B型淀粉粒体积百分比,其中灌浆中、后期弱光影响程度较前期大.表明,弱光条件下小麦籽粒淀粉合成底物优先供应淀粉粒的生长,而非形成更多的淀粉粒. 相似文献
14.
15.
Parental, F(1) , F (2) , BC (1) and BC (2) generations of four crosses involving four cultivars of durum wheat (Triticum durum Desf.) were evaluated at two sites in Tunisia. A three-parameter model was found inadequate for all cases except crosses Chili x Cocorit 71 at site Sidi Thabet and Inrat 69 x Karim at both sites. In most cases a digenic epistatic model was sufficient to explain variation in generation means. Dominance effects (h) and additive x additive epistasis (i) (when significant) were more important than additive (d) effects and other epistatic components. Considering the genotype-by-environment interaction, the non-interactive model (m, d, h, e) was found adequate. Additive variance was higher than environmental variance in three crosses at both sites. The estimated values of narrow-sense heritability were dependent upon the cross and the sites and were 0%-85%. The results indicate that appropriate choice of environment and selection in later generations would increase grain protein content in durum wheat. 相似文献
16.
Homogenates of 7-day-old S. alba seedlings hydrolysed cholesteryl[4-H14C] β-d-glucoside or sitosteryl β-d-glucoside-[6-3H]. Activity was located predominantly in the cell membrane structures sedimenting at 1000–15 000 g and was solubilized by acetone treatment. Partially purified enzyme preparation, with an about 1500 times higher specific activity with respect to the crude homogenate, was obtained by repeated acetone precipitation and subsequent chromatography on DEAE-Sephadex and Sephadex G-100. During this procedure a considerable separation from other enzymes with β-glucosidase activity was achieved. The enzyme had MW 65 000 daltons, pH optimum at 5.2–5.6. Two observations suggested that the enzyme was a specific steryl β-d-glucoside hydrolase. Firstly, there was no substrate competition between steryl glucosides and several other β-d-glucosides. Secondly, enzyme activity wasstrongly inhibited by low concentrations of various 3β-OH sterols with a planar ring system and an intact side chain. 相似文献
17.
The uptake and distribution of eight metallic elements were examined in wheat seedlings for a period of 12 d with a radioactive
multitracer technique. The radioactive nuclides of the seedlings were simultaneously determined by γ-ray spectrometry. All
of the elements studied were taken up by the wheat seedlings and mainly accumulated in the roots. Only some elements were
transported to shoots and leaves of the seedlings or bound to leaf proteins, and two elements were transported into the chloroplast.
Uptake of most elements reached a maximum on the fifth or the eighth day and then gradually decreased afterward. In the cases
of 95mTc and 72Se, the uptake increased continuously within 12 d without the peak uptake. The change of elemental concentrations was dependent
on uptake and excretion rates. The dynamics of metal elements taken up by the wheat seedlings and their distribution in roots,
shoots, and leaves were different for each element, suggesting that it may depend on the characteristics of the elements. 相似文献
18.
通过响应面法优化提取发酵麸皮多糖的工艺,并评价其体外益生和抗氧化活性。以发酵麸皮多糖的得率为响应值,采用纤维素酶酶解与水浴浸提相结合的方法提取发酵麸皮多糖,以纤维素酶添加量、料液比、水浴浸提温度、水浴浸提时间为试验因素建立数学模型,筛选最佳提取工艺条件。通过测定还原力、DPPH和·OH自由基的清除能力对比发酵和未发酵麸皮多糖的体外抗氧化活性,并通过测定嗜酸乳杆菌、植物乳杆菌、两歧双歧杆菌的生长对比发酵和未发酵麸皮多糖的体外益生活性。结果表明,发酵麸皮多糖最佳提取工艺为:料液比1∶16(w/v),酶添加量1 000 U/g,水浴浸提温度90℃,水浴浸提时间60 min,在此条件下发酵麸皮多糖的得率实测值为73. 35%。发酵麸皮多糖具有较强的DPPH和·OH自由基的清除能力,可促进嗜酸乳杆菌、植物乳杆菌和两歧双歧杆菌的生长。 相似文献
19.
Rice and wheat brans, without additional nutrients and hydrolyzed by alpha-amylase and amyloglucosidase, were fermented to DL-lactic acid using a newly isolated strain of Lactobacillus sp. RKY2. In batch fermentations at 36 degrees C and pH 6, the amount of lactic acid in fermentation broth reached 129 g l(-1) by supplementation of rice bran with whole rice flour. The maximum productivity was 3.1 g lactic acid l(-1) h(-1) in rice bran medium supplemented with whole rice flour or whole wheat flour. 相似文献
20.
小麦土霉素抗性内生细菌分离、鉴定及分布 总被引:1,自引:0,他引:1
研究通过培养的方法研究了小麦根际土壤、根、茎、叶各部位的土霉素抗性内生菌数量、种类和分布特征.结果表明,小麦内生菌数目在1×104cfu g-1~1.95×106 cfu g-1 之间,土霉素抗性内生菌数目介于6.9×103cfu g-1和5.67×105 cfu g-1,从中分离出22株土霉素抗性内生菌,包括15株G+和7株G-,经16s rRNA基因序列分析,它们与Bacillaceae,Alphaproteobacteria 和Gammaproteobacteria 三大类微生物聚在一起,其中Bacillaceae类细菌最多,占59.1%.土霉素抗性的Bacillus licheniformis在小麦根际土壤、根、茎等器官中都分离得到表明该抗性菌种在小麦内生系统中具有较高的传播性;具有土霉素抗性的人类机会致病菌Bacillus cereus和Stenotrophomonas属的细菌在小麦的根际土壤、根以及叶子中也均分离到,但是在小麦种子中没有分离到任何土霉素抗性的内生菌. 相似文献