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1.
对畲族血浆组特异性成分(Gc)、红细胞磷酸葡萄糖变位酶1(PGM1)、酸性磷酸酶(AcP1)、6-磷酸葡萄糖酸脱氢酶(6-PGD)、腺苷脱氨酶(ADA)、腺苷酸激酶(AK1)的遗传多态性进行了研究。Gc与PGM1是用薄层聚丙烯酰胺凝胶等电聚焦分析的,AcP1、6-PGD、ADA及AK1是用淀粉凝胶电泳分析的。各基因座的基因频率分别为Gc*1F0.4722、Gc*1S0.2421、Gc*20.2738;PGM1*1A0.5357、PGM1*1B0.1627、PGM1*2A0.1587、PGM1*2B0.1429;AcP*1A0.1825、AcP1*B0.8175;6-PGD*A0.9683、6-PGD*B0.0278;ADA*10.9881、ADA*20.0119;AK1*11.0000。畲族Gc、PGM1、AcP1、6-PGD和ADA基因为多态,而AK1基因为单态。发现1例带有6-PGD*R和3例带有Gc*1A2变异等位基因的个体,其中Gc*1A2基因频率为0.0119,达到多态水平。 相似文献
2.
用淀粉凝胶电泳法对我国汉族9个人群的红细胞酸性磷酸酶、酯酶、及6-磷酸葡萄糖脱氢酶的遗传多态性进行了研究。研究结果表明:兰州、呼和浩特,哈尔滨,西安,郑州,成都,贵阳,漳州,梅州等9市汉族人群的AcP1^B基因频率依次为0.929、0.8167、0.7938、0.8131、0.8088、0.8005、0.7896、0.7794和0.7675;EsD^1基因频率依次为0.6473、0.6148、0. 相似文献
3.
对畲族血浆组特异性成分、红细胞磷酸葡萄糖变位酶1(PGM1)、酶性磷酸酶(AcP1)、6-磷酸葡萄糖酸氢酶(6-PGD)、腺苷脱氨酶(ADA0、腺苷酸激酶(AK1)的遗传多态性进行了研究。Gc与PGM1是用薄层聚丙烯酰胺凝胶等电聚集分析的,AcP1、6-PGD、ADA及AD1是用淀粉凝胶电泳分析的。各基因座的基因频率分别为Gc1F0.4722、Ge1S0.2421、Gc20.2738;PGM11A 相似文献
4.
经SephadexG-75凝胶过滤,QAE-SephadexA-50和CM-SephadexC-25离子交换层析的步骤,从湖南产尖吻蝮(Dienagkistrodonacutus)蛇毒中纯化出两个出血毒素(DaHT-1和DaHT-2).SDS-PAGE测得分子量均为23.5kD,IEF-PAGE测得等电点分别为5.6和5.2,两者具有相似的氨基酸组成,其中酸性氨基酸(Asx,Glx)分别占23%和24%,DaHT-1和DaHT-2的最小出血剂量(MHD)分别为0.5μg和0.8μg。都具蛋白水解酶活性,无对TAME,BAEE的水解活性和PLA2酶活性.两者的蛋白水解酶活力与出血活性并非正相关.DaHT-1和DaHT-2的最适温度分别为35℃和40℃,最适pH为6-9,对热均不稳定,温度高于60℃活性完全丧失。金属离子的分析显示每摩尔毒素蛋白约含0.5mol的Zn,1mol的Ca,较多的Na、K、Mg,不含Co。 相似文献
5.
地衣芽孢杆菌β—甘露聚糖酶的纯化及酶学性质 总被引:9,自引:1,他引:9
地衣芽孢杆菌(Bacillus licheniformis)NK-27菌株发酵产生的β-甘露聚糖酶(β-mannanase)经硫酸铵盐析沉淀,两次DEAE纤维素和Sephadex G-100离子交换柱层析以及制备PAGE等步骤,获得了凝胶电泳均一的样品。用SDS-凝胶电泳测得纯化后的β-甘露聚糖酶分子量为26kD,用凝胶聚焦电泳测得等电点P1为5.0。酶反应的最适pH为9.0,最适温度为60℃,稳 相似文献
6.
地衣芽孢杆菌1Baciuus Licheniformis)BL-306产生的胞外β-甘露聚糖酶经硫酸铵分级盐析,DEAE-纤维素柱层析。Sephadex-G100柱凝胶过滤和DEAE-纤维素柱再层析分离纯化,得到SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)均一样品。用SDS-PAGE测得纯化后β-甘露聚糖酶分子量为26000道尔顿。用凝胶等电聚焦电泳(PAGEIEF)测得等电点PI为5.0。该酶 相似文献
7.
将构建好的可表达GST融合蛋白的重组病毒AcMNPV-OCC^--GST-6xHis-Etp28感染Sf9细胞,一定时间后取感染了病毒的细胞裂解物上清液进行SDS-PAGE分析,结果显示53kDa的融合蛋白(GST-6xHis-Etp28)呈不溶状态。在原有裂解液的基础上,加固体十二烷基肌氨酸钠致终浓度1.5%,并将Triton X-100的比例由1%提高到2%。SDS-PAGE结果显示至少有1/ 相似文献
8.
用蛋白质工程方法改变葡萄糖异构酶最适pH和最适温度 总被引:3,自引:2,他引:3
用寡核苷酸诱导的定点突变方法构建了葡萄糖异构酶基因的突变体(N184D和A198C)。含突变体的重组质粒pTKD-GI1(N184D)和pTKD-GI2(A198c)在E.coliK38菌株中表达,用DEAE-Sepharose FF和Sephacryl S-300HR柱层析分离纯化突变酶。与野生型葡萄糖异构酶比较实验表明:(1)突变酶N184D的最适pH值下降了1个单位;等电点下降了0.6个单位 相似文献
9.
10.
调查了102名云南阿昌族的ABO,NNSs,Rh和P系统的红细胞血型,结果表明,阿昌族的基因频率p(0.3874)是迄今国内调查过人群中的最高值,E(0.2459)和CDe(0.6936)基因或染色体频率较高,而S(0.0686)和P1(0.1089)频率较低;Ms(0.5950)连锁率高于Ns(0.3353);未发现SS和Rh(-D)阴性表现型。 相似文献
11.
十一个少数民族红细胞酸性磷酸酶、酯酶D、6-磷酸葡萄糖酸脱氢酶及谷丙转氨酶的遗传多态性 总被引:2,自引:1,他引:1
用淀粉凝胶电泳法对我国十一个少数民族红细胞酸性磷酸酶(AcP_1)、酯酶D(EsD)、6-磷酸葡萄糖酸脱氢酶(6-PGD)及谷丙转氨酶(GPT)的遗传多态性进行了研究,共调查了2272人。研究结果表明:侗、回、白、土家、苗、彝、藏、满、瑶、哈尼和布依等民族AcP_1~B基因频率依次为0.7835、0.7958、0.8137、0.7750、0.7624、0.8038、0.8075、0.8035、0.7725、0.6488和0.6896;EsD~1基因频率依次为0.6418、0.7315、0.6005、0.6025、0.6411、0.6411、0.6558、0.6305、0.6020、0.6023和0.6368;6-PGD~A基因频率依次为0.9279、0.9381、0.9387、0.9150、0.9356、0.9014、0.7764.0.8818、0.9851.0.9233和0.9410;GPT~1基因频率依次为0.4075、0.5367、0.5049、0.4824、0.5322、0.6106、0.6313、0.6400、0.3985、0.4930和0.3976。并对发现的变异型进行了讨论。 相似文献
12.
中国20个民族中PGM_1及其亚型EsD、GLO1、AK、ADA和6-PGD酶型分布调查 总被引:2,自引:0,他引:2
调查了汉族、鄂伦春、赫哲、朝鲜、蒙古、羌、土家、苗、侗、畲、壮、纳西、傈僳、白、彝、景颇、哈尼、傣、维吾尔和塔吉克等20个民族的PGM_1及其亚型,EsD、GLO_1、AK、ADA和6-PGD等酶型的分布及基因频率。PGM_1及其亚型、EsD和GLO_1在中国各民族中是分布较好的,个人识别能力较高的酶。有12个民族查出有PQM_1~6基因,壮族的频率最高,PGM_1 6-1表型达4.15%。对在4174份血样中所检出的带有PGM_1~6基因的68份血样做亚型分析,在凝胶上PGM_1~6谱带均在同一位置上。EsD_1基因频率的总趋向是北方各民族高于南方。哈尼、傈僳、傣、纳西、畲、壮、侗和苗等民族EsD2-2表型达15%以上,哈尼族高达32.4%。GLO1~1基因频率塔吉克和维吾尔族为0.2927和0.2112,羌族为0.0583,其它各族在0.0714—0.1527。各民族AK~1、ADA和6-PGD~(?)基因频率均甚高。 相似文献
13.
The geographical distribution of the gene frequencies from loci: Hp, Tf, Gc, Pi, AcP1, GLO1, EsD, 6-PGD, PGM1 and RFLP's of the nuclear DNA of the loci HBG-2 (HindIII), HBB (AvaII), ApoB (XbaI), D7S8 (PstI), LDLR (HincII) and AT-3 was analysed in the Mongolian population. These data revealed the homogeneity of 18 local groups in Mongolia and extremely low genetic differences measured by GST. There was no differences in the average GST values between protein markers and nuclear DNA markers. 相似文献
14.
H W Goedde H G Benkmann D P Agarwal L Hirth U Bienzle M Dietrich H H Hoppe J Orlowski E Kohne E Kleihauer 《American journal of physical anthropology》1979,50(2):271-277
Population genetic studies in Saudi Arabia are performed for EsD, GPT, AcP, ADA, AK, 6-PGD, PGM, C3, Tf, Hp, Gc, Pi, Bf, Hb, ABO-blood groups and Rh-factor, level of the third component of complement and immunoglobulins. The data are compared with reported frequencies in European and African populations. 相似文献
15.
According to integral characterization of gene frequencies of the investigated loci AB0, MN, Rh, GLO1, PGM1, EsD, AcP, 6-PGD, Hp, Tf, Gc, C'3 and ChE2, Mongolian population has high level of polymorphism, with the exception of haplotypes R" (cdE) and Ry(CdE) at the Rh locus and TfB0-1 at the Tf locus. The data on biochemical and immunological polymorphic gene markers analysed in the population of Mongolia show that the Mongolians have some distinctive features, in comparison with the mean-in-the-world characteristics: high frequencies of the B genes at the AB0 locus; D, E, R1 and R2 at the Rh locus; GLO11, PGDc, TfDChi, E2(C5+), PGM1(1+); low frequencies of the genes A(AB0), R0(Rh), AcPc, Hp1, Gc2, C'3F, PGM 1(2-); the rest of the genes at the above-mentioned loci and the genes of the locus MN have the mean-in-the-world frequencies. 相似文献
16.
Distribution of the genetic markers of blood groups (AB0, MNSs, Rhesus, P, Lewis, Duffy, Kell-Cellano, Kell-Kp, Kell-Sutter, Kidd, Lutheran); of serum proteins (Hp, Tf, Gc, C'3, Pi); red-cell enzymes (6-PGD, EsD, GLO, AcP, subtypes of PGM) was studied in Karels from the South part of Karelian ASSR. The results of comparison of Karels with the other finno-ugric peoples revealed peculiarities of gene pool in Karelian population. 相似文献
17.
A sample of the population from Central Sardinia was studied with respect to acid phosphatase (AcP) and esterase D (EsD) enzymes. The gene frequencies were: AcPA = 0.322, AcPB = 0.617, AcPC = 0.061 and EsD1 = 0.892. The results were compared with those of other Italian populations. 相似文献
18.
A sample of the South Sardinia population was studied with respect to acid phosphatase (AcP) and esterase D (EsD) enzymes. The gene frequencies were: AcPA = 0.326, AcPB = 0.607, AcPC = 0.067 and EsD1 = 0.883. The results were compared with those of other Italian populations. 相似文献
19.
Luiselli D Simoni L Tarazona-Santos E Pastor S Pettener D 《American journal of physical anthropology》2000,113(1):5-17
A sample of 141 Quechua-speaking individuals of the population of Tayacaja, in the Peruvian Central Andes, was typed for the following 16 genetic systems: ABO, Rh, MNSs, P, Duffy, AcP1, EsD, GLOI, PGM1, AK, 6-PGD, Hp, Gc, Pi, C3, and Bf. The genetic structure of the population was analyzed in relation to the allele frequencies available for other South Amerindian populations, using a combination of multivariate and multivariable techniques. Spatial autocorrelation analysis was performed independently for 13 alleles to identify patterns of gene flow in South America as a whole and in more specific geographic regions. We found a longitudinal cline for the AcP1*a and EsD*1 alleles which we interpreted as the result of an ancient longitudinal expansion of a putative ancestral population of modern Amerindians. Monmonnier's algorithm, used to identify areas of sharp genetic discontinuity, suggested a clear east-west differentiation of native South American populations, which was confirmed by analysis of the distribution of genetic distances. We suggest that this pattern of genetic structures is the consequence of the independent peopling of western and eastern South America or to low levels of gene flow between these regions, related to different environmental and demographic histories. 相似文献
20.
我国汉族人群的乙二醛酶Ⅰ遗传多态性 总被引:1,自引:0,他引:1
用琼脂糖平板凝胶电泳技术分析了我国六个城市的汉族共1238人的红细胞乙二醛酶I的遗传多态性。每个汉族人群的受检人数和GLO1*1基因频率分别为:郑州200人(0.1023),哈尔滨195人(0.1103),呼和浩特210人(0.1259),贵阳204人(0.1373),兰州210人(0.1476),西安199人(0.1508)。中国不同地区汉族人群之间的GLOI基因频率分布存在显著性差异,但这种差异明显小于中国不同少数民族之间的差异。 相似文献