Evaluating agro-industrial by-products as dietary roughage source on growth performance of fattening steers

Silages from pineapple peel, sweet corn husk and cob mixed with bagasse and vinasse were evaluated to determine their chemical composition and fermentation characteristics as well as feeding performance in fattening steers. The experiment, which lasted 90 days, involved 48 fattening steers (264 ± 37.4 kg BW) randomly allocated to three diets. Treatments included: a control diet containing rice straw and molasses (T1); diet containing bagasse–vinasse mixture including sweet corn husk and cob silage (BS; T2); and diet containing bagasse–vinasse mixture including pineapple peel silage (BP; T3). All treatments included a commercial concentrate feed (13% CP) and ad libitum rice straw throughout the experiment. Results from chemical analysis showed that dry matter (DM) of BS was higher than BP (P < 0.05), whereas the protein content of BS and BP was similar (P > 0.05). For fermentation characteristics, pH in BP was lower than BS (P < 0.05); in addition, acetic and butyric acids in BS were higher than BP (P < 0.05). Findings from growth trial showed that total DM intake in steers fed T1 was higher compared to the other dietary treatments (P < 0.05), whereas the average BW gain was found to be grater in T3 steers (P < 0.05). As result from our findings, bagasse–vinasse mixture with pineapple peel silage appeared to be a viable feed ingredient in fattening steer diet and moreover it could become an economically feasible agro-industrial by-product for farmers.     

Effects of mulberry leaves to replace rapeseed meal on performance of sheep feeding on ammoniated rice straw diet

This study was conducted to investigate effects of leaves of mulberry tree (Morus alba) as a protein supplement to isonitrogenously replace rapeseed meal (RSM) on performance of growing lambs offered ammoniated rice straw (ABRS) (Trial 1), and to evaluate the digestive characteristics of the ABRS supplemented with different ratios of RSM and mulberry leaves in terms of in vitro gas production (Trial 2). In Trial 1, 45 Huzhou lambs were divided into five equal groups according to their body weight and gender. Lambs in each group were kept in three pens (male, female and mixed (one male and two females)), and received one of the following dietary treatments: 100 g RSM (A), 75 g RSM plus 60 g mulberry leaves (B), 50 g RSM plus 120 g mulberry leaves (C), 25 g RSM plus 180 g mulberry leaves (D), and 240 g mulberry leaves (E). All animals were given ABRS ad libitum along with 100 g ground corn per head per day. The intake of ABRS was slightly increased with the supplementary level of mulberry leaves, and hence total intake increased with the increasing level of mulberry leaves. The growth rates were higher in diets A and E than those in other treatments (P<0.05), with little difference between diets A and E, and the slowest in C. Animals of all genders showed a similar trend, though male lambs was higher in weight gain than the female. While feed efficiency was higher in diet A, concentrate consumption per kilogram of weight gain was lower when higher level of mulberry leaves was supplemented (diets D and E). Feed cost per kilogram gain was lower in diets E and A compared to other treatments. Degradation of dry matter in the rumen of sheep were higher for mulberry leaves than for RSM, but crude protein was less degraded for mulberry leaves than for RSM. The potential GP was significantly higher in diet A than those in B, C and D (P<0.05), and higher in E than in C (P<0.05) (Trial 2), indicating a negative associate effect of mulberry leaves and RSM on digestion. It is inferred that mulberry leaves may be used as a protein supplement to ammoniated straw diets to fully substitute for RSM, but these two supplements should unlikely be supplemented together to avoid the negative associate effect.     

Hemicellulose sugar recovery from steam-exploded wheat straw for microbial oil production

There are currently few successful examples of using straw hemicellulose as a carbon source in the fermentation industry. In this paper, hemicellulose hydrolysates were recovered from steam-exploded wheat straw (SEWS) and used to produce microbial oil. The effects of the steam explosion treatment conditions, the elution temperature and the ratio of elution water to SEWS on sugar recovery were examined. A broth with 3.8 g l^?1 of reducing sugar and 22.3 g l^?1 of total soluble sugars was obtained with a 10-fold excess (w/w) of water at 40 °C to wash the SEWS treated under steam explosion conditions at 200 °C for 5 min. This broth was used to produce microbial oil by the oleaginous fungus Microsphaeropsis sp., which was able to secrete xylanase to degrade oligosaccharides from straw hemicellulose and accumulate microbial oil. Under optimized conditions, the oil concentration was 2.6 g l^?1. The yield of oil from sugar consumed was 0.14 g g^?1. The microbial oil produced by this research could be used as feedstock for biodiesel production because the microbial oil was primarily composed of neutral lipids. This research establishes a novel protocol for microbial oil production from straw hemicellulose.     

Supplementation of isonitrogenous oil seed cakes in cactus (Opuntia ficus-indica)–tef straw (Eragrostis tef) based feeding of Tigray Highland sheep

The experiment was conducted at Maichew Agricultural Technical Vocational Education and Training College, Ethiopia. Twenty four male yearling Tigray Highland sheep with mean body weight (BW) of 21 ± 2.6 kg (mean ± S.D.) were used to investigate the effect of different protein sources on feed intake, nutrient digestibility, BW change and carcass parameters in a study comprising of 90 days feeding trial, followed by 7 days of digestibility trial and evaluation of carcass parameters. Six individually fed animals were used per treatment in a randomized complete block design. The treatments consisted of ad libitum feeding of tef (Eragrostis tef) straw plus 172 g dry matter (DM) of cactus (Opuntia ficus-indica) pear (T1, control) and supplementation with 145 g DM cotton seed cake (CSC) (T2), 195 g DM noug seed cake (NSC) (T3) or 149 g DM peanut cake (PNC) (T4) per head per day. The quantity of the supplements was set to supply 62.5 g crude protein (CP). Tef straw DM intake was depressed (P<0.01) as the result of NSC supplementation. Sheep supplemented with CSC and PNC had higher (P<0.001) total DM intake than the control and NSC supplemented ones. Supplementation with NSC and PNC also resulted in higher (P<0.01) apparent digestibility of DM and OM compared to the control treatment. Supplementation with CSC and PNC resulted in better daily BW gain (P<0.001), feed conversion efficiency (FCE) and dressed carcass weight (P<0.01) compared to the non-supplemented diet. Dressing percentage on slaughter weight base was higher (P<0.01) in supplemented sheep than in the non-supplemented ones. Supplementation with PNC also promoted higher (P<0.05) rib-eye muscle area than in the non-supplemented ones. It was concluded that supplementation with 145 g DM CSC and 149 g DM PNC resulted in better feed intake, BW gain and carcass traits in cactus–tef straw based feeding of sheep.     

Influence of undegraded intake protein on intake,digestion, serum hormones and metabolites,and nitrogen balance in sheep

In Exp. 1, 20 ewes of mixed breeding were fed grass hay : straw mixtures, assigned to one of four supplemental treatments and evaluated during six collection periods. Supplemental treatments were control (no supplement), and low, medium, and high levels (5.2%, 22.1%, and 41.3% of DM, respectively) of undegraded intake protein (UIP). Supplements were formulated to be similar in degraded intake protein (DIP; 21%). Digestibilities of DM, OM, and CP were increased (P < 0.10) with protein supplementation and in medium and high compared with low UIP supplemented ewes. Digestibility of CP also was increased (P < 0.10) in ewes on high compared with medium treatments. Serum insulin was not influenced (P > 0.10) by UIP treatment, except in collection period four. In contrast, serum glucose and growth hormone were not influenced (P > 0.10) by UIP treatment. In Exp. 2, four wether lambs fed the same treatments as in Exp. 1, were used in two 4 × 4 Latin squares trials. In trial 1 lambs received a grass hay diet (6.7% CP) and in trial 2 lambs were fed 40 : 60 blend (6.6% CP) of grass hay and spring wheat straw. In both trials, N intake, urinary N, N digestion, apparent N absorption, and N retention were increased (P < 0.10) with protein supplementation. In addition, in trial 1, urinary N, N digestion, and apparent N absorption were increased (P < 0.10) in medium and high compared with low UIP and also in high compared with medium UIP treatments. In trial 2 of Exp. 2, total feed intake (g/kg BW), digestibility of DM and OM, BW, and fecal N were increased (P < 0.10) with protein supplementation. Organic matter digestion, BW, N intake, and N retention were increased by medium and high compared with low UIP. Fecal N, BW, N intake, and N retention were increased (P < 0.10) in high compared with medium UIP. These data indicate that increasing levels of UIP supplementation increases DM, OM, and CP (N) digestibility, serum urea N concentration, and N retention in sheep fed low quality forage.     

The effect of replacing rice straw with dry wormwood (Artemisia sp.) on intake,digestibility, nitrogen balance and ruminal fermentation characteristics in sheep

This study examined the effect of substituting dry wormwood (Artemisia sp.) for rice straw in sheep diets on intake and apparent digestibility in vivo, nitrogen (N) balance and ruminal fermentation characteristics. Four Corriedale×Polwarth sheep (51.7 ± 1.3 kg) were individually housed in metabolism cages and fed diets (ad. libitum) with a 70:30 forage to concentrate ratio (DM basis), in which the basal rice straw was substituted with 0 (Control), 30 (LW), 50 (MW) or 100 (HW) g/kg of dry wormwood. The experimental design was a 4×4 latin square design in which 10 days of dietary adaptation was followed by 6 days of total feces and urine collection in each period. Rumen fluid was collected from a stomach tube at −0.5, 0.5, 1, 2, 4, and 8 h after the morning feed on day 6 of each collection period and analyzed for volatile fatty acids (VFA). The intakes of dry matter (DM), organic matter intake (OM), crude protein (CP), ether extract (EE), neutral detergent fibre (NDF) and acid detergent fibre (ADF) were higher (P<0.05) in sheep fed diets containing wormwood, than those fed the Control diet. Compared to the Control diet, CP digestibility was higher (P<0.05) in sheep fed MW and N intake, retained N, EE digestibility, urinary purine derivatives and microbial N yield were higher (P<0.05) in sheep fed diets, containing wormwood. Rumen pH was unaffected by treatment. Rumen NH₃-N and VFA concentrations were similar across treatments except that most values for diets containing wormwood were higher (P<0.05) than those for the control diet within the first 2 h of feeding. The non-glucogenic acid ratio was also similar across Control, LW and MW treatments, but it was generally lower in MW versus the Control treatment. In conclusion, substituting wormwood for rice straw in the sheep diets increased feed intake, rumen fermentation, in vivo digestibility, N retention and microbial N yield, particularly at the medium and high levels of wormwood inclusion.     

Does soil nutrient availability influence night-time water flux of aspen saplings?

Previous investigations have been demonstrated that night-time water flux may increase or remain unchanged at low mineral nutrient availability. At the same time, it is a well-known fact that night-time water flux is relatively high in fast-growing species, which typically grow in fertile soil. To test the impact of soil nutrient deficiency on night-time water flux and fine-root acclimation in saplings of hybrid aspen (Populus tremula L. × Populus tremuloides Michx.), a growth chamber experiment was carried out. We set up a hypothesis that night-time sap flux density (F) and night-time water use percentage from daytime water use (NWU) are more intense in fertile conditions, in order to enhance or sustain the high intrinsic growth potential of hybrid aspen. The main limiting element in the low nutrient availability treatment (low-n) was nitrogen. The nitrogen concentrations of leaves and fine-roots exhibited the strongest (R² = 0.95; P < 0.001) positive relationship with NWU and foliar biomass. Both the night-time F and NWU were several times higher in the case of fertilized soil (high-n treatment) compared to low-n treatment (P < 0.01). The differences in nocturnal (and in diurnal) F disappeared at the end of the period of sap flow measurements, when the foliage area of trees was almost full-grown. Endogenous increase in water flux during predawn hours was observable only in the high-n treatment. Significantly greater NWU (P < 0.01) and specific fine-root length (P < 0.05), but smaller fine-root biomass (P < 0.05) in saplings of the high-n treatment potentially allow plants to use mass flow in soil more efficiently for transportation nutrients towards roots and to decrease construction costs for fine-root biomass production. Our results suggest that decreased night-time water flux as a result of strong nutrient (especially nitrogen) deficit could be characteristic to fast-growing tree species, which are adapted to grow in fertile soil.     

Designing better water troughs: 2. Surface area and height,but not depth,influence dairy cows’ preference

Water consumption affects milk production of dairy cows. In a previous study, we found that dairy cows preferred to drink from larger than from smaller troughs and that intake was higher when water was offered in the larger, preferred troughs. In this study, we investigated some of the trough's characteristics that may underlie such preference. The volume of water consumed, time spent drinking and number of sips taken by cows (n = 18) were compared when water was offered in two troughs differing in surface area (1.13 m² or 0.28 m²; experiment 1), height (30 cm or 60 cm; experiment 2) or depth (30 cm or 60 cm; experiment 3). In each experiment, each cow was tested individually for six consecutive days with the troughs randomly placed in each side. In experiment 1, cows took more sips (P < 0.01), spent more time drinking (P < 0.01) and drank more water (P < 0.01) from the trough with larger surface area. In experiment 2, cows took more sips from the higher than from the lower trough (P < 0.02) and showed a tendency to consume more water (P = 0.08) and to spend more time drinking (P = 0.08) from the higher than from the lower trough. Trough depth did not influence any of the variables recorded.     

Behavioural responses of sows and piglets from two genotypes to recorded nursing grunts played throughout lactation

The impact of playbacks of recorded sow nursing grunts on the development of nursing behaviour in sows from two genotypes was studied. Yorkshire × Landrace (YL; n = 16) and 25% Meishan (MH; n = 16) gilts were equally divided in two groups: (1) no playback, and (2) playbacks of recorded sow nursing grunts at 35-min intervals. Recordings were played from day 110 of gestation until day 27 of lactation. Sow behaviour was videotaped for 24 h every 4 days, from days 6 to 26 of lactation. Playbacks decreased nursing intervals in MH (34.9 min versus 38.2 ± 1.1 min) without altering it in YL (37.9 min versus 36.5 ± 1.1 min; breed × treatment, P < 0.05), although this effect in MH was likely due to the increased frequency of non-productive nursings seen on days 6, 10 and 14 (P < 0.05) in sows subjected to playbacks. In both breeds, the interval between nursings increased (P < 0.01) from day 6 (33.7 ± 0.8 min) to 26 (40.8 ± 0.8 min) of lactation, the interval between non-productive nursings (NPN) remained similar (P > 0.1) and sows, compared to piglets within a litter, ended more nursings as lactation advanced (P < 0.01). More nursings were terminated by sows in MH than in YL (P < 0.01) whereas more nursings were terminated by piglets from YL than MH sows (P < 0.05). Playbacks or genotype did not alter the amount of time over a 24 h period that sows spent standing, sitting, lying on the belly or lying with the udder exposed (P > 0.1), but sows spent more time sitting, standing and lying on the belly and less time lying with the udder exposed as lactation advanced (P < 0.01). Posture changes were more frequent for MH than YL sows (P < 0.01) and increased as lactation advanced in both genotypes (P < 0.01). In conclusion, the effects of playbacks on nursing behaviour differed between genotypes: playbacks decreased nursing intervals in MH sows only, although this was likely due to an increase in NPN.     

Clinical profile of sheep fed non-conventional feeds containing phenols and condensed tannins

A study was carried out to investigate the effects of feeding low quality non-conventional feeds (NCF) containing phenols and condensed tannins on the clinical profiles of sheep. Thirty-two Omani sheep were fed one of four diets with two base roughages, urea-treated palm frond (UTPF) and Rhodesgrass hay (RGH) and two concentrates, commercial concentrate (CC) and a by-products concentrate (BC) for 120 days. Haematological, serum biochemical and urine analyses were used to assess sheep health. Non-conventional feeds (urea-treated palm frond and by-products concentrate) contained higher levels of polyphenols and condensed tannins than conventional feeds (Rhodesgrass hay and commercial cubes). Feeds based on urea-treated palm frond had lower dry matter, crude protein, acid detergent fibre, neutral detergent fibre, gross energy (P < 0.001) and ash (P < 0.05) digestibility coefficients than those based on Rhodesgrass hay. Animals fed NCF had lower feed intake (P < 0.001) and lower body gain (P < 0.001) than those fed conventional ones. They also produced larger volumes of faeces (P < 0.01) which contained higher levels of nitrogen (P < 0.001) and had lower viscosity values of intestinal content (P < 0.001). Rumen liquor of NCF-fed animals had higher pH and lower ammonia–nitrogen levels (P < 0.01). Animals fed urea-treated palm frond plus by-products concentrate had lower lymphocyte (P < 0.01), monocyte (P < 0.05) and eosinophil (P < 0.05) counts by the end of the trial than those fed Rhodesgrass hay based diets. The urea-treated palm frond and by-products concentrate fed animals had lower blood urea nitrogen (BUN) levels (P < 0.05), higher (P < 0.01) alanine aminotransferase (ALT) and lower serum iron (P < 0.001) than those fed Rhodesgrass hay based diets. There was a trend of increasing blood, leukocytes and specific gravity in the urine of NCF-fed animals. This experiment implies that feeding low quality non-conventional feeds containing antinutritional factors for relatively long periods might produce subtle negative effects on the physiology and chemistry of the digestive system and blood parameters which might negatively affect sheep health and make them more susceptible to diseases.     

Effect of hydrolytic lignin on formation of protein–lignin complexes and protein degradation by rumen microbes

Several methods have been developed to protect feed protein from rumen microbial degradation. The current study aimed to evaluate the potential use of an industrial lignin, namely hydrolytic lignin, to protect protein from rumen microbial degradation. The hydrolytic lignins assessed in this study were extracted from wheat straw previously subjected to various steam treatment conditions (pressure: 15, 17 and 19 bar; reaction time: 0, 5 and 10 min; use of acidic catalyst: without and with 2% H₂SO₄ on DM basis). Results indicated that hydrolytic lignin can precipitate protein when measured by a standard bovine serum albumin assay. It was also observed that protein-precipitating capacity of lignin increased with increasing harshness of steam treatment until a point from which no further effect was observed. The effect of lignin upon protein degradation in vitro was clearly detected. Both ammonia nitrogen and iso-acid concentration in vitro were significantly decreased (P<0.01) when lignin was added to fermentation flask containing casein. Unlike tannins, hydrolytic lignins do not inhibit rumen microbial activity. Additionally, it was observed that lignin’s ability to bind and protect protein is a pH-dependent reaction. Protein binding to lignin is markedly reduced at pH<3.0.     

Effect of barley and oats on feed intake,live weight gain and some carcass characteristics of fattening lambs

The influence of whole, crushed and ground barley and oats on intake, performance and carcass characteristics of lambs was examined. A comparison of diets was conducted with individually-fed male and female lambs in six groups. The study was repeated in three successive years (1995–1997) using the same experimental design. A total of 192 lambs of a mean initial age of 74 days (SD 13.0) and live weight 20.6 kg (SD 4.54) were daily fed their respective concentrates, 72 g dry matter (DM)/kg metabolic live weight (kgW^0.75), for 98 days from weaning to slaughter. Hay was provided ad libitum.The lambs adapted more quickly to diets containing barley than to those containing oats. Compared to oats, the total daily DM intake was higher on barley (1162 vs. 990 g DM/lamb, 89 vs. 78 g DM/kgW^0.75, P < 0.001). Hay consumption was significantly lower on oats than on barley (182 vs. 344 g DM/day, P < 0.001). On oats the lambs experienced energy and protein deficiencies with their energy and protein intakes being 20% below feeding recommendations. On the barley diets the energy and protein requirements of the lambs were satisfied. When processed cereals were offered, there was no increase in the total DM, energy or protein intakes. Processing did not improve the organic matter digestibility of barley or oats.The growth rate (P < 0.01), carcass weight (P < 0.001) and slaughter percentage (P < 0.001) were higher on a barley diet than on oats. A barley diet resulted in more kidney fat (P < 0.01) and fattier carcasses. Processing had no positive influence on the live weight gain. On the contrary, lambs receiving crushed or ground barley or oats had a lower final live weight than those receiving whole grains (P < 0.001). The utilisation of whole grain was more efficient than that of processed grain. There were no advantages in processing barley and oats for the feeding of lambs.     

Variability of soil carbon sequestration capability and microbial activity of different types of salt marsh soils at Chongming Dongtan

Variations in the soil carbon sequestration capability of different types of salt marsh soils at Chongming Dongtan and its influencing factors were studied by analyzing the soil organic carbon (SOC) content, organic matter input and microbial activities. The results indicated that the total SOC content at Area A (southeast of Dongtan, sandy soil with Phragmites communis) was only 46.11% of that of Area B (northeast of Dongtan, clay soil with mixed P. communis and Spartina alterniflora) (P = 0.000 < 0.05), but their organic matter input per year was almost identical. These findings implied that Area B had a lower output of SOC. The microbial biomass at Area A was 3.83 times greater than that at Area B (P = 0.049 < 0.05); the soil catalase and invertase activities at Area A, which were related to carbon metabolism, were 60.31% (P = 0.006 < 0.05) and 34.33% (P = 0.021 < 0.05) higher than at Area B, respectively; and the soil respiration at Area A was also higher than at Area B. These findings implied that the microbial activities at Area A were greater than those at Area B, and therefore the carbon metabolism was rapid, resulting in increased SOC output at Area A. Increased water content and salinity in the clay soil at Area B may inhibit the microbial activities, thereby reducing the decomposition of the organic matter and enhancing carbon sequestration. In addition, some artificial measures for controlling spread of S. alterniflora at Area B (mowing/digging and tillage (M + D); mowing/digging and tillage/waterlogging (M + D + W)) were found to generally improve the microbial activity of soil, thereby increasing SOC output. However, when the two different physical controlling modes were compared, the SOC and microbial activities of the soil subjected to the M + D + W treatment were relatively high and low, respectively, due to waterlogging restraining the microbial metabolism. These findings indicated that the difference in microbial activities was the important factor leading to variability in the SOC sequestration capability between Areas A and B. Additionally, with the exception of soil texture and vegetation types, environmental conditions and artificial turbulence also influenced microbial activities of soil, and hence SOC output and organic carbon sequestration capability.     

Effect of cold process monensin enriched urea molasses mineral blocks on performance of crossbred calves fed a wheat straw based diet

Twenty male crossbred calves were divided into four equal groups. Calves in groups I and II were fed wheat straw ad libitum with a concentrate mixture with or without monensin (30 mg per day per animal). Calves in groups III and IV were fed wheat straw ad libitum with 70% of the allocated concentrate mixture and had free access to urea molasses mineral block (UMMB) with or without monensin (100 ppm). Wheat straw intake was higher (P<0.05) in UMMB supplemented groups, but total dry matter (DM) and crude protein (CP) intake did not differ. ME (Mcal per day) intake was higher (P<0.05) in UMMB supplemented groups. Digestibility of DM, OM, EE, and NDF did not differ due to UMMB or monensin supplementation, although ADF digestibility was increased (P<0.01) with UMMB supplementation. Although the N balance was similar among the groups, the Ca and P balances were higher in UMMB supplemented groups. Blood glucose level was increased (P<0.05) due to monensin treatment but plasma urea N level did not differ. Average body weight gain, feed conversion efficiency, protein utilisation efficiency, and energy utilisation efficiency were higher (P>0.05) in monensin treated groups without any change in body composition. Replacing 30% of a concentrate mixture with a cold process UMMB increased the proportional contribution of wheat straw to DM intake but had no effect on animal performance. However, supplementation with monensin increased the blood glucose level, protein and energy deposition, as well as body weight gain and feed efficiency, but with no change in the wheat straw and total DM consumption.     

Novel extraction method of antioxidant compounds from Sasa palmata (Bean) Nakai using steam explosion

Antioxidant compounds were extracted from various parts of Sasa palmata (Bean) Nakai, a bamboo plant whose leaves are commonly used to wrap foodstuffs such as Sushi in Japan. Highest concentrations of antioxidant compounds existed in the leaf part of S. palmata. Steam explosion treatment followed by hot water and methanol extractions was used for separating the antioxidant compounds from S. palmata leaf. The steam explosion treatment is the physical–chemical treatment which crushes a sample by sudden reduction of the pressure in reactor to atmospheric pressure after steaming the sample at high temperature and pressures. Sasa palmata leaf was hydrolyzed by steaming and crushed by the rapid decompression. The optimal condition of steam explosion for the effective extraction of antioxidant compounds from S. palmata was determined as a steam of temperature of 250 °C and a steaming time of 1 min. In these conditions 217.41 mg/(g-Sasa leaf) of phenolic compounds and 142.81 mg/(g-Sasa leaf) of radical scavenging activity, that was expressed as butylated hydroxyanisole (BHA), were obtained.     

The influence of drinker location and colour on drinking behaviour and water intake of newborn pigs under hot environments

The effect of drinker location within the farrowing crate in relation to its microenvironment and the effect of dispenser colour on drinking behaviour and water intake of newborn pigs for the first two days of their lives were studied. In the first trial, 16 sows were randomly assigned to four treatment groups with the piglet's water dispenser placed in the front right (FR), front left (FL), back right (BR) or back left (BL) corner of the farrowing pen, respectively. In the second trial nine sows were randomly assigned to three treatment groups with the piglets’ water dispenser, placed in the front left corner of the farrowing pen, and coloured as red (CR), green (CG) or blue (CB), respectively. Trials commenced between 08:00 and 10:00 h on the first morning after the litters were born and the piglets’ age was considered to be zero (“age”) by that time.The average age of the piglets at their first visit to water dispensers was 15.9 ± 1.8 h. The newborn pigs visited the drinker more frequently (P < 0.001) during daytime than during night, with two peaks soon after sow feeding time. The duration of drinking time was long at the time of higher (noon) and shorter at the time of lowest (early morning) temperatures (P < 0.01) in the farrowing house, respectively. The greatest attention at the drinkers occurred soon after suckling (P < 0.001). The longest time spends at the drinker during the first visits and decreased as the pigs visited dispensers repeatedly. The profile of visit frequency and duration differed between piglet sexes (P < 0.001). The pigs that did not visit the drinker grew slower and were lighter at 48 h of age than the pigs visited the drinker. First visit and overall mean visit age were significantly shorter for BL than for FR and BR groups. Piglets visited the FL drinker for shorter time than the others. Water consumption per pig/24 h was lower for FL and higher for BL groups (P < 0.05). Water consumption was higher for CR and CB groups than for CG group. The overall mean visit age was shorter for CG than for CB group. Significant interactions were observed between sex and colour groups for the number of visits (P < 0.05). The results of the present study revealed that water dispenser use by newborn piglets is characterised by diurnal and between two successive milk consumptions distributions. Water consumption by piglets from birth to 48 h of their lives is strongly influenced by water dispenser location in the farrowing pen and the dispenser colour. It also appears that males and females behave differently to the three colour dispensers used in this study.     

Growth,intake, diet digestibility,and nitrogen use in three hair sheep breeds fed alfalfa hay

Pen feeding and metabolism trials were conducted to determine intake, diet digestibility and nitrogen (N) use in three hair sheep breeds with differing growth potential offered an alfalfa hay diet. For pen feeding, 24 6-mo-old wether lambs, equally representing the Barbados Blackbelly, Katahdin, and St. Croix breeds, were paired by breed, placed in 12 enclosed cement-floor pens (2.5 m × 3.5 m), and offered chopped alfalfa (Medicago sativa L.; 17.6% CP, 50.4% NDF, and 36.4% ADF) ad libitum. Lambs were allowed a 14-d adaptation period to pens and diets, and remained on trial for 56 d. Starting body weight (BW) was different (P < 0.05) among breeds (Barbados Blackbelly: 23.8 kg; Katahdin: 36.5 kg; St. Croix: 29.1 kg). Daily gain was not different (P < 0.10) between Katahdin (131 g/d) and St. Croix (117 g/d), but both were higher (P < 0.05) than Barbados Blackbelly (87 g/d). Daily dry matter hay intake was similar among breeds (107–109 g/kg BW^0.75), and feed to gain ratio ranged from 8.7 in St. Croix and 9.1 in Katahdin to 10.5 in Barbados Blackbelly, but was not different (P > 0.10) among breeds. Six lambs per breed were used in the metabolism trial. Total DMI was greater (P < 0.01) for Katahdin (1196 g/d) than St. Croix (907 g/d) and Barbados Blackbelly (858 g/d), but was not different (P > 0.10) adjusted for body weight (mean: 71 g/kg BW^0.75). Coefficients of apparent digestibility for DM (P < 0.06), OM (P < 0.06), N (P < 0.05), NDF (P < 0.08), and ADF (P < 0.08) were higher for Katahdin compared to Barbados Blackbelly with St. Croix intermediate. Intake of N (P < 0.01), feces N (P < 0.08), urine N (P < 0.01), absorbed N (P < 0.01), and retained N (P < 0.05) also were greater for Katahdin compared to St. Croix and Barbados Blackbelly. Blood glucose, urea-N, and creatinine were not different (P > 0.10) among breeds. Breeds differed in their growth performance in line with breed mature size, and differences were observed in N digestibility and absorption.     

Mitochondrial distribution patterns in canine oocytes as related to the reproductive cycle stage

This study investigates the mitochondrial (mt) distribution in canine ovarian oocytes examined at recovery time, as related to the reproductive cycle stage, and in oviductal oocytes. Ovarian Germinal Vesicle (GV) stage oocytes were recovered from bitches in anestrous (A, n = 2), follicular phase (F, n = 4), ovulation (O, n = 2), early luteal (EL, n = 7) and mid/late luteal phase (MLL, n = 2). Oviductal GV, metaphase I (MI) or MII stage oocytes were recovered from six bitches between 56 and 110 h after ovulation. Mitochondria were revealed by using MitoTracker Orange CMTM Ros and confocal microscopy. In ovarian oocytes, three mt distribution patterns were found: (I) small aggregates diffused throughout the cytoplasm; (II) diffused tubular networks; (III) pericortical tubular networks. Significantly higher rates of oocytes showing heterogeneous mt patterns (II + III) were obtained from bitches in F (75%) and in O (96%) compared with bitches in A (31%; F vs. A: P < 0.05; O vs. A: P < 0.001), in EL (61%; O vs. EL: P < 0.01), or in MLL (0%; F vs. MLL: P < 0.05; O vs. MLL: P < 0.001). Fluorescence intensity did not vary according to mt distribution pattern except that it was lower in oocytes recovered in EL phase and showing small mt aggregations (P < 0.001). The majority of ovulated MII stage oocytes (79%) showed diffused tubular mt network. We conclude that mt distribution pattern of canine ovarian immature oocytes changes in relation to reproductive cycle stage and that patterns observed in oocytes recovered from bitches in periovulatory phases are heterogeneous and similar to those of in vivo matured oocytes.     

Nutrient composition,digestion and rumen fermentation in sheep of wheat straw treated with calcium oxide,sodium hydroxide and alkaline hydrogen peroxide

This study tested the effect of calcium oxide (CaO), sodium hydroxide (NaOH) and NaOH plus hydrogen peroxide (H₂O₂; AHP) on cell wall composition, digestion and fermentation of wheat straw (straw) in sheep. Treated straws were prepared by mixing straw either with water followed by dusting with CaO at 160 g kg⁻¹ DM or with a NaOH solution alone at 3 l kg⁻¹ DM to supply 80 g NaOH kg⁻¹ DM (Na) or pre-soaked with Na exactly as in the previous treatment for 27 h followed by mixing with 130 g H₂O₂ kg⁻¹ DM (AHP) for 6 h. After 14 days of storage, the treated straws and an untreated straw (U) were fed automatically every 2 h to four individually housed sheep together with a supplement in a 4×4 latin square experiment. Each kilogram supplement DM contained 422 g CP and 10.8 MJ ME. NDF (p<0.001) and hemicellulose (p<0.01) contents were significantly reduced whereas cellulose was increased (p<0.001) in treated compared to untreated straw. ADL was reduced in Ca (p<0.05) but increased (p<0.05) in Na and AHP compared with U. The rumen and total tract digestibility were significantly (p<0.001) greater in sheep fed treated compared with untreated straw. Significant differences (p<0.05) between treatments for pH, NH₃ and VFA were also observed. All treatments improved the nutritive value of straws compared with untreated through modification of cell wall with a subsequent increase in digestibility by sheep. Although the digestibility for Ca was lower than that for Na despite reduction in cell wall, its use to treat straws may be more safe and cost effective than Na. AHP was the most effective and could also improve the energy value of other low quality forages for ruminants. However, the need of AHP for a large amount of NaOH to achieve highly alkaline pH limits its farm scale application. Therefore, further studies should either consider reducing the amount of NaOH or finding alternative alkalis that are cost effective and user-friendly.     

Influence of different types of environmental enrichment on the behaviour of finishing pigs in two different housing systems: 3. Hanging toy versus rootable toy of the same material

This study aimed to assess the effects of (1) environmental enrichment with either straw bedding or a plastic toy and (2) the way in which the plastic toy was presented on the behaviour of finishing pigs in two different housing systems. One thousand and twenty four (Large White × Landrace) × Large White pigs were housed contemporarily in either a straw-based (ST) or fully slatted (FS) building from 35 kg to slaughter at 104 kg. In each building, half of the pens received additional environmental enrichment in the form of a hanging plastic toy. The remaining pens in the ST house were enriched only by the straw bedding. In the FS house, the remaining pens were provided with a rootable toy of the same plastic material presented on the floor of the pen. There was no significant effect of housing system on the level of manipulation of the hanging toy. Within the FS system, the level of manipulation of the hanging toy tended to be higher than that of the plastic toy presented on the floor (P = 0.052). Neither form of additional environmental enrichment provided a comparable level of occupation to that of straw bedding. In the absence of straw, more investigatory behaviours were directed towards both pen-mates (P < 0.001) and pen components (P < 0.001). Further study is required to identify functional forms of environmental enrichment in slatted systems to occupy pigs and prevent them from performing adverse behaviours.