共查询到20条相似文献,搜索用时 171 毫秒
1.
2.
3.
4.
Girschick HJ Grammer AC Nanki T Mayo M Lipsky PE 《Journal of immunology (Baltimore, Md. : 1950)》2001,166(1):377-386
It has been suggested that B cells acquire the capacity for secondary V(D)J recombination during germinal center (GC) reactions. The nature of these B cells remains controversial. Subsets of tonsil and blood B cells and also individual B cells were examined for the expression of recombination-activating gene (RAG) mRNA. Semiquantitative analysis indicated that RAG1 mRNA was present in all tonsil B cell subsets, with the largest amount found in naive B cells. RAG2 mRNA was only found in tonsil naive B cells, centrocytes, and to a lesser extent in centroblasts. Neither RAG1 nor RAG2 mRNA was routinely found in normal peripheral blood B cells. In individual tonsil B cells, RAG1 and RAG2 mRNAs were found in 18% of naive B cells, 22% of GC founder cells, 0% of centroblasts, 13% of centrocytes, and 9% of memory B cells. Individual naive tonsil B cells containing both RAG1 and RAG2 mRNA were activated (CD69(+)). In normal peripheral blood approximately 5% of B cells expressed both RAG1 and RAG2. These cells were uniformly postswitch memory B cells as documented by the coexpression of IgG mRNA. These results indicate that coordinate RAG expression is not found in normal peripheral naive B cells but is up-regulated in naive B cells which are activated in the tonsil. With the exception of centroblasts, RAG1 and RAG2 expression can be found in all components of the GC, including postswitch memory B cells, some of which may circulate in the blood of normal subjects. 相似文献
5.
6.
7.
8.
Lynch RL Konicek BW McNulty AM Hanna KR Lewis JE Neubauer BL Graff JR 《Molecular cancer research : MCR》2005,3(3):163-169
9.
Armoni M Harel C Karni S Chen H Bar-Yoseph F Ver MR Quon MJ Karnieli E 《The Journal of biological chemistry》2006,281(29):19881-19891
10.
11.
12.
13.
14.
15.
Ig gene rearrangement steps are initiated in early human precursor B cell subsets and correlate with specific transcription factor expression 总被引:3,自引:0,他引:3
van Zelm MC van der Burg M de Ridder D Barendregt BH de Haas EF Reinders MJ Lankester AC Révész T Staal FJ van Dongen JJ 《Journal of immunology (Baltimore, Md. : 1950)》2005,175(9):5912-5922
16.
17.
18.
19.
20.
《Cellular signalling》2014,26(3):564-569
Gastric cancer remains the main cause of cancer related deaths all over the world, and upregulated COX2 is a key player in its development. The mechanism as to how COX2 is regulated during the gastric cancer development is largely unknown. In this study, we found that the expression of COX2 was closely correlated with NF-κB activity. Strikingly, NF-κB activity was not absolutely consistent with its nuclear localization. Especially, in some cancer cell lines, such as MKN28, there were abundant nuclear localized NF-κB, while NF-κB luciferase activity in this cell line was relatively low. Furthermore, FOXP3 was found to be abundantly expressed in these cells. When the nuclear localized NF-κB expression was adjusted with the expression of FOXP3, it then correlated well with NF-κB activity. Molecularly, increased FOXP3 expression can interact with NF-κB and thus repress its activity. Knockdown of FOXP3 could increase NF-κB activity, COX2 expression, and cell migration. Taken together, our study revealed that function of FOXP3 as a negative regulator of NF-κB activity and thus plays a tumor suppressor role by reducing cell metastasis. 相似文献