Micronutrient uptake and distribution in mycorrhizal or phosphorus-fertilized soybeans

Summary Soybean plants were grown in a soil very low in available P. Seedlings were inoculated with two vesicular-arbuscular mycorrhizal (VAM) fungi or were left non-inoculated and fertilized with P. Assimilation and allocation of micronutrients (Fe, Mn, Zn, and Cu) were determined during host development, and the uptake of trace elements in VAM plants was compared to P-fertilized, non-VAM plants of similar weight, growth stage, and P status. Copper and zinc concentrations were always higher in VAM plants, while iron and manganese concentrations were lower than in the equivalent P-fertilized soybeans. Differences in the micronutrient content of fully-mature soybean pods reflected differences in the leaves and roots. Thus, for trace elements, seed quality can be altered by VAM colonization in a fashion not duplicated by P fertilizer. Contribution from the Western Regional Research Center, USDA-ARS (CRIS No. 5325-20580-003).     

Effects of the different levels of dietary trace elements from organic or inorganic sources on growth performance,carcass traits,meat quality,and faecal mineral excretion of broilers

ABSTRACT

This experiment was conducted to evaluate the effects of different sources and levels of trace elements on growth performance, carcass composition and mineral excretion levels of broilers. In a completely randomised experimental design, 900 one-day-old male Ross-308 broilers were assigned to 5 treatments, with 6 replicates of 30 birds each. The control group (CITE) was fed with a basal diet containing regular inclusion levels of inorganic trace elements. Treatment groups were supplied with reduced levels (30% and 50% of the regular level) of inorganic (ITE) or organic trace elements (OTE), respectively. Groups 50% ITE, 30% OTE and 50% OTE diets had equivalent average daily gain (ADG), average daily feed intake (ADFI), feed to gain ratio (F/G ratio) and mortality rate compared with group CITE in any phase. However, compared with group CITE chicks in group 30% ITE have lower ADG and ADFI and higher F/G ratio. The carcass yields were not affected by dietary treatments. Compared with group CITE, in groups 30% ITE, 50% ITE, 30% OTE and 50% OTE the shear force values of the breast muscle were only 71.8%, 83.4%, 63.5% and 59.4% (p < 0.05), respectively. Birds received diets containing reduced levels of trace elements had diminished excretions of Mn and Zn throughout the entire period (p < 0.01). In conclusion, the reduced supplementation of trace elements had no or slightly negative impact on growth performance, carcass yield and meat quality, but decreased faecal mineral excretion. Moreover, the trace element supply as OTE played a limited role on performance and excretion and was only partly beneficial for animal performance in case the trace element supply was reduced to 30%.     

Serum trace elements status of rabbits supplemented with Nigella sativa,vitamins C and E,and selenium against damage by N-methyl-N'-nitro-N-nitrosoguanidine

In this study, we investigated the effects of Nigella sativa, vitamins C and E, and selenium on the levels of trace elements in the serum of N-methyl-N′-nitro-N-nitrosoguanidine (MNNG)-injected rabbits. The rabbits were separated into one control and three experimental groups, each consisting of eight rabbits. MNNG was administered to all rabbits at a dose of 20 mg/kg. Group A received a suspension of N. sativa, group B received a combination of vitamins C and E and selenium, and group C received MNNG without any additional treatment. Group D did not receive any treatment and acted as control. The concentrations of serum zinc, copper, and iron were determined for groups A, B, C, and D. The zinc levels were 155.3±25.8, 304.7±14.22, 117.2±27.9, and 87.0±8 μ/dL for groups A-D, respectively; copper was measured at 234.8±31.9, 214.3±14.2, 196.5±19.3, and 359.2±19.9 μ/dL and iron levels were 276.3±10.71, 260.8±7.15, 211.2±13.47, and 223.4±9.5 μ/dL, in the stated group order. There were statistically significant differences between groups (p<0.05). The results obtained in this work may be of use for monitoring and preventing the nocive effects of N-methyl-N′-nitro-N-nitrosoguanidine and similar carcinogens.     

氮磷肥对茶树锌硒等中微量元素吸收与分配的影响

锌（Zn）和硒（Se）及其他中微量元素（铝Al,钙Ca,铁Fe,铜Cu,锰Mn）是茶叶品质的重要指标,但茶树吸收Zn、Se能力及氮（N）磷（P）肥影响中微量元素吸收与分配的过程尚不清楚。以红壤丘陵区福鼎大白茶树为研究对象,开展Zn+Se、Zn+Se+N、Zn+Se+P、Zn+Se+N+P和对照共5种处理3次重复随机化区组试验,处理第3年春季分茶叶、成熟叶、吸收根、运输根和储藏根采集植物样品,测定其元素含量。结果表明,茶树地上和地下器官Zn和Se及其他中微量元素对N、P、Zn、Se添加的响应具分异性。与对照相比,茶树地上和地下器官Zn和Se含量均显著增加,与Zn+Se相比,施N和/或P肥仅显著提高茶叶和成熟叶Se含量（P<0.05）;与对照相比,施肥处理均显著提高吸收根和运输根Al、Fe含量以及储藏根Cu含量;运输根Mn含量表现为Zn+Se+N、Zn+Se+P、Zn+Se+N+P显著高于对照,储藏根Mn含量为Zn+Se+N+P显著高于其他处理;茶树各器官Ca含量对施肥处理无显著响应。此外,茶叶和成熟叶的Zn含量与吸收根显著正相关,而Se含量则与储藏根显著正相关。茶树具有吸收和积累Zn和Se的能力,而施N、P肥有助于提高茶叶Se含量,研究结果为红壤丘陵区培育高品质锌硒茶及营建生态高值茶园提供了依据。     

Mineral contents of Aloe vera leaf gel and their role on streptozotocin-induced diabetic rats

The role of some inorganic elements like vanadium, zinc, sodium, potassium, calcium, copper, manganese, and traces of chromium in the improvement of impaired glucose tolerance and their indirect role in the management of diabetes mellitus are being increasingly recognized. In traditional methods, medicinal plants are being used, which contain both organic and inorganic constituents. In the present study, an attempt has been made to analyze the inorganic elements present in Aloe vera leat gel and their role on diabetes-related biochemical alterations in experimental rats. Special emphasis was given to the inorganic parts by carefully preparing ash of the leaf gel. The results clearly indicate the presence of several hypoglycemic-activity-possessing elements in the gel. The ash treatment also resulted in hypoglycemic action. In conclusion, the presence of various inorganic trace elements in the gel might account for the hypoglycemic nature of the plant.     

Sorption and desorption of trace elements bySpartina alterniflora detritus

Element and extractant-specific desorption of Mn, Fe, Cu, and Zn fromSpartina alterniflora detritus was observed. Desorption of a substantial fraction of the total detrital Mn, Fe, Cu and Zn occured rapidly when the detritus was treated with 0.1N HCl or 1 MgCl₂. This treatment removed precipitated/coprecipitated and adsorbed trace elements, respectively, suggesting that a large fraction of detritus-bound trace elements are in readily exchangeable, surface reaction sites. The carboxylic acid functional group cation exchange capacity of the detritus also suggests an important role of surface exchange reactions in the dynamics of trace elements during decomposition. The rate and magnitude of changes in the trace element content of detritus has important implications for estuarine biogeochemical cycling of these elements including the potential for biological uptake of trace elements by detritus-consuming fauna.This research was supported by a cooperative agreement between the National Marine Fisheries Service and the US Department of Energy. The opinions or assertions contained herein are the private views of the authors and are not to be construed or reflecting the views of the Department of the Army or the Department of Defense. Special thanks to Dr. W. L. Johnson who provided the proximate analyses, Southeast Fisheries Center Contribution No. 82-10B.     

Trace fossils of the arthropod Camptophyllia from the Westphalian (Carboniferous) rocks of Lancashire, UK and their palaeoenvironmental context

Two arthropod trace fossils are described and analysed from the Carboniferous Lower Westphalian (C. communis and basal A. modiolaris chronozones) coal-bearing strata of Lancashire. The biserial trackway Diplichnites triassicus consists of five overlapping en echelon sets of 7–9 tracks preserved as epichnia and hypichnia in lacustrine siltstones. The trackway suggests subaqueous in-phase walking by a multi-segmented producer with a body length of 35–40 mm, width 17–22 mm, and 7–9 appendages. Curved, clustered, or laterally repeated, hypichnial lobes with transverse striations on the base of ripple cross-laminated sandstone are identified as Rusophycus versans. This trace fossil is interpreted as shallow resting or furrowing burrows of a homopodous arthropod, 30–60 mm long, 15–30 mm wide, and probably the same kind of arthropod as produced D. triassicus.A review of contemporary arthropod body fossils from Lagerstätten in Lancashire favours the onisciform, or Arthropleura like arthropod Camptophyllia as a potential producer of both of these trace fossils in a lacustrine palaeoenvironment.This study integrates the analysis of sediments, trace fossils and body fossils for reconstructing the arthropod biota and ecology in Westphalian lacustrine and crevasse splay fluvial palaeoenvironments.     

Influence of age, sex, and sexual activity on trace element levels and antioxidant enzyme activities in field mice (Apodemus sylvaticus and Mus spretus)

The influence of age, gender and sexual activity on both hepatic levels of some trace elements (Fe, Cu, Zn, Mn, Se) and the activities of glutathione-S-transferase (GST) and superoxide dismutase (SOD) was investigated in Wood mice (Apodemus sylvaticus) and Algerian mice (Mus spretus). Animals were taken from a riverside community of an unpolluted area of central Portugal. Adult A. sylvaticus presented the highest hepatic mean concentrations of Cu and Mn, whereas adult M. spretus had the highest Fe concentration in the liver. Moreover, an influence of age on the contents of Fe, Zn, and Mn has been observed in A. sylvaticus, whereas in M. spretus an influence of gender and sexual activity was only detected on Zn levels. In contrast, enzyme activities were not influenced by the studied variables, despite a tendency for an increase in SOD activity in sexually active M. spretus. GST activity was species dependent, whereas SOD activity was similar between species. These findings were analyzed regarding the relationship of both essential trace elements and the two antioxidant enzymes with physiological and metabolic pathways related to life cycles in the two species of mice. Results enhanced the understanding of A. sylvaticus and M. spretus as biological models, allowing their future use as bioindicators of environmental toxicity.     

Concentration levels of major and trace elements in rice from sri lanka as determined by the k 0 standardization method

Instrumental neutron activation (INAA) with k ₀ standardization has been used to determine the concentration levels of a variety of major and trace elements (Al, As, Br, Cd, Cl, Co, Cr, Cs, Cu, Fe, Hg, K, La, Mg, Mn, Mo, Na, Rb, Sc, Se, and Zn) in rice grains (raw and parboiled) and in rice flour collected from local markets in Sri Lanka. In addition, the energy-dispersive X-ray fluorescence (EDXRF) analysis has been used to determine the three elements Ca, P, and S in powdered samples. To evaluate and assure the accuracy and precision of the k ₀ standardization method, the IAEA standard reference material V-8 rye flour was analyzed. The results obtained in the present investigation were compared with the results reported from other countries. All of the elements detected in the rice matrices from Sri Lanka were very low in concentration or within normal limits for food plants. Approximate daily dietary intakes of the individual elements supplied through rice were calculated and compared with the available literature values of daily allowances.     

Histochemical properties and trace element concentrations in <Emphasis Type="Italic">Parietaria L.</Emphasis>from urban sites (Palermo,Italy)

We examined leaf and pollen histochemical properties in Parietaria L. and measured trace element concentrations in urban sites in Palermo, Sicily, affected by different stress valence levels. Urban pollution affects histochemistry macro-, micro-, and toxic element concentrations in Parietaria. We therefore decided to determine whether this plant can be used as a simple biomonitor or bioaccumulator of pollution contaminants. Samples were collected at various sites in different periods. An increase in phenolic compound levels was a result of the impact of pollution. Histochemical characteristics and trace element concentrations varied according to the period, but not to the place, of sampling. Pollutant concentrations were the highest in the month of February 2002. Trace metal concentrations were the lowest in July. Our investigations have confirmed that Parietaria L. can be used to study the effects of trace elements in the atmosphere in urban sites; in fact, they are veritable accumulators, given that elevated levels of pollutants can be found on their structures with no visible morphological effects. There are differences in accumulation capacity according to the trace elements studied. Pollution alters the content of phenolic compounds and chemical elements in Parietaria L.     

Seasonal changes of zinc, copper, and iron in gilthead sea bream (Sparus aurata) fed fortified diets

Four groups of gilthead sea bream (Sparus aurata) were fed diets with additional metal contents: a basal diet (diet A) contained Zn at 60.9 ± 1.9 mg/kg diet, Cu at 3.9 ± 0.9 mg/kg diet, and Fe at 138.3 ± 6.8 mg/kg diet; the other diets were supplemented with copper (20 mg/kg, diet B), iron (100 mg/kg, diet C), or zinc (300 mg/kg, diet D). Two consecutive year-classes (0⁺ and 1⁺ age fish) from the same parent stock were examined. Several fish tissues were analyzed for metal contents in five different periods of each year in order to determine (1) the sensitivity of certain tissues as indicators of trace element metabolism and (2) possible seasonal variations. Growth data were similar for gilthead sea bream fed the basal diet and the metal-fortified diets. Mineral concentrations in tissues were found to be little affected by the dietary supplementation of trace elements, suggesting an efficient homeostatic control of these three metal concentrations. Tissues involved in metal metabolism (e.g., liver, kidney, gills) presented greater variations between minimum and maximum values with respect to other tissues (e.g., brain, muscle, eye). Seasonal variations were observed during the 2 yr of this study and were especially evident for zinc and copper concentrations in the liver. The overall pattern of metal variations showed a decreasing trend during the 2 yr. Results from this study indicate that (1) trace element concentrations in fish tissues vary with age and life cycle and (2) trace element requirements may vary in function of age and life cycle.     

Aluminum,Fe, Ca,Mg, K,Mn, Cu,Zn and P in above- and belowground biomass. II. Pools and circulation in a subalpineAbies amabilis stand

Elemental concentrations of above- and belowground tissues were determined in anAbies amabilis stand in the Cascade Mountains, Washington, USA. These data were used to calculate the pools and circulation of trace elements and micronutrients on a stand level. For all elements except Al, a greater proportion (from 62 to 87%) was distributed in above- rather than belowground tissues. This contrasted sharply with the biocirculation of elements where 97% of the Al and Fe, 88% of the Cu and 67–84% of the Ca, P, and Mg of total detrital cycling was from the belowground components. Aboveground tissues, however, contributed 69% of the Zn, 65% of the K and 68% of the Mn found in annual detritus production. The proportion of total element pool circulated annually was the highest for Al (82%) and Fe (32%) followed by 13% and less for the remaining elements. Copper, Fe and Al were accumulated in root tissues, while Mn and Zn accumulated in foliage.We hypothesize that roots are an effective mechanism for avoiding Al toxicity in these subalpine ecosystems. The large root biomasses of these stands allow for high Al levels to be taken up and immobilized in roots; this is observed in the significantly higher Al accumulations in below- than aboveground tissues. The high root turnover in these stands is hypothesized to be a result of root senescence occurring in response to high Al accumulation. Furthermore, Al inputs into detritus production occur by soil horizon so that roots with high Al concentrations located in the Bhs horizon turnover and are retained within that horizon. These roots also decompose very slowly (99% decay = 456 years) due to the high Al and low Ca, Mn and Mg present in these tissues and therefore have very little impact on short-term elemental cycling.     

Determination of main and trace element contents in human brain by NAA and ICP-AES methods

A study was undertaken to determine the average values for elements in normal human brain (11 individuals, age group 65–75). Twelve brain parts were selected from both hemispheres. Determinations were carried out by NAA and ICP-AES. The main elements (Na, K, Mg, Ca, Fe, P, S) and trace elements (Al, B, Co, Cr, Cu, Mn, Ni, Pb, Zn) were investigated. Quality control was ensured by using NBS Bovine Liver SRM. The results obtained with independent methods were compared, and the data show a good correlation. On the basis of these investigations, the regional distribution of elements can be given.     

The inventories of anthropogenic Ph,Zn, As,Cd, and the radionuclides 137Cs and excess 210Pb in lake sediments of the Adirondack region,USA

We determined the inventories of four anthropogenic trace elements, Pb, Zn, As, and Cd, and two radionuclides, ¹³⁷Cs and excess ²¹⁰Pb, in sediment cores collected from eight remote lakes in the Adirondack region of the northeastern United States. The inventories of all six substances vary considerably among the sediment cores, although the lakes and their associated catchments must have received similar cumulative per unit area atmospheric inputs of these substances. These variations are highly correlated, indicating that the trace elements and radionuclides are affected in a coherent way by the processes controlling their deposition to the sediments of these lakes. Assuming that the anthropogenic trace element inventories in each sediment core are enhanced or depleted relative to cumulative atmospheric deposition to the extent indicated by the sediment core inventories of either ¹³⁷Cs or excess ²¹⁰Pb, we produced estimates of the cumulative atmospheric inputs of the four anthropogenic trace elements to this region. Comparison of the excess ²¹⁰Pb normalized anthropogenic Pb, Zn, Cd inventories of the Adirondack sediment cores with excess ²¹⁰Pb normalized inventories of the same substances in a group of South Central Ontario lakes suggests that the Adirondack region has received greater cumulative anthropogenic inputs of Pb, Zn and Cd by a factor of 1.6, 4.5, and 2.9, respectively.     

Three new insertion sequence elements ISLdl2, ISLdl3, and ISLdl4 in Lactobacillus delbrueckii: isolation,molecular characterization,and potential use for strain identification

A group of new insertion sequence (IS) elements, ISLdl2, ISLdl3, and ISLdl4, from Lactobacillus delbrueckii subsp. lactis ATCC 15808 was isolated, characterized, and used for strain identification together with ISLdl1, recently characterized as an L. delbrueckii IS element belonging to the ISL3 family. ISLdl2 was 1367 bp in size and had a 24 bp IR and an 8 bp DR. The single ORF of ISLdl2 encoded a protein of 392 aa similar to transposases of the IS256 family. ISLdl3 had a single ORF encoding a protein of 343 aa similar to transposases of the IS30 family. Finally, ISLdl4 had a single ORF encoding a protein of 406 aa and displayed homology to the transposases of the IS110 family. ISLdl4 was only slight different from ISL4 (Accession No. AY040213). ISLdl1, ISLdl2, and ISLdl4 were present in all of the 10 L. delbrueckii subsp. lactis and subsp. delbrueckii strains tested, as well as in three of the 11 L. delbrueckii subsp. bulgaricus strains tested. ISLdl3 was present only in four closely related strains of L. delbrueckii subsp. lactis. These IS elements were not observed in Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus helveticus, or Lactobacillus plantarum. A cluster of IS elements, ISLdl1, ISLdl2, ISLdl3, ISLdl4, and ISL6, was observed in L. delbrueckii subsp. lactis strain ATCC 15808. Within this cluster, ISLdl4 was inserted into ISLdl1 between the left IR and the start codon of ORF455, encoding a putative transposase. Most of the integration sites of the IS elements were strain-specific. We have observed that IS elements can migrate from one strain to another as integral parts of bacterial DNA by using phage LL-H as a vehicle. We demonstrate for the first time that inverse PCR and vectorette PCR methods with primers based on sequences of the IS elements could be used for identification of L. delbrueckii strains.     

Effects of Aluminum Exposure on Bone Mineral Density,Mineral, and Trace Elements in Rats

The purpose of the study was to investigate the effects of aluminum (Al) exposure on bone mineral elements, trace elements, and bone mineral density (BMD) in rats. One hundred Wistar rats were divided randomly into two groups. Experimental rats were given drinking water containing aluminum chloride (AlCl₃, 430 mg Al³⁺/L), whereas control rats were given distilled water for up to 150 days. Ten rats were sacrificed in each group every 30 days. The levels of Al, calcium (Ca), phosphorus (P), magnesium (Mg), zinc (Zn), iron (Fe), copper (Cu), manganese (Mn), selenium (Se), boron (B), and strontium (Sr) in bone and the BMD of femur were measured. Al-treated rats showed lower deposition of Ca, P, and Mg compared with control rats. Levels of trace elements (Zn, Fe, Cu, Mn, Se, B, and Sr) were significantly lower in the Al-treated group than in the control group from day 60, and the BMD of the femur metaphysis in the Al-treated group was significantly lower than in the control group on days 120 and 150. These findings indicate that long-term Al exposure reduces the levels of mineral and trace elements in bone. As a result, bone loss was induced (particularly in cancellous bone).     

Effects of antioxidant vitamins A, C, E and trace elements Cu, Se on CuZn SOD, GSH-Px, CAT and LPO levels in chicken erythrocytes

The biologically damaging effects of reactive oxygen species are controlled in vivo by a wide spectrum of antioxidant defence mechanisms. Dietary constituents of antioxidant vitamins and trace elements may play an important role in protecting against oxidant damage. The effects of supplementation of vitamins A, C, E and trace elements Cu and Se on the activities of antioxidant enzymes and lipid peroxide levels in chicken erythrocytes were investigated depend on the time. CuZnSOD activity and plasma Cu levels in the Cu group were increased by 39 and 37 per cent respectively. CuZnSOD activity in vitamin C groups was also increased by 20 per cent. The GSH-Px activity in Se, Se+E and Se+Cu groups was raised by 35, 46 and 69 per cent respectively. Also, the GSH-Px activity in the vitamin C group was increased by 33 per cent. Catalase activity in all of these groups was not significantly different when compared with controls (p<0.01). The maximum decrease in LPO levels of 42 per cent was obtained for the Se+E group.     

Distribution of two Agrobacterium tumefaciens insertion elements in natural isolates: Evidence for stable association between Ti plasmids and their bacterial hosts

Summary A large number of Agrobacterium tumefaciens strains of the biotype III group carry two to ten copies of two related IS elements, IS866 and IS867. A study of the distribution and localization of these elements in 54 strains showed that one IS866 and two IS867 copies are always found at characteristic sites on the octopine/cucumopine and vitopine Ti plasmids, whereas varying amounts of IS866 and IS867 copies occur at different positions on the chromosome. By comparison of the IS patterns, an evolutionary tree could be deduced which shows the phylogenetic relationships between 23 different types of Agrobacterium strains. The structures of the T-regions of the different strains were also compared. Within the octopine/cucumopine group, eight T-region patterns could be defined. These patterns were found to be correlated with the chromosomal IS patterns. This strongly suggests that the IS866 and IS867 containing Ti plasmids are stably associated with their bacterial hosts. The possible role of the IS866 and IS867 elements in Ti plasmid evolution is discussed.     

Concentrations of copper, iron, and zinc in the major organs of the wistar albino and wild black rats

The concentrations of copper, iron, and zinc in the major organs of Wistar albino (Rattus norvegicus) and wild black rats (Rattus rattus) were measured by means of atomic absorption spectroscopy. The copper levels in the kidneys and liver of the Wistar albino rats (WARs) were significantly higher (p<0.05) than in the wild black rats (WBRs). There were no significant differences in the concentrations of zinc in the liver, lungs, kidneys, and brain between the two study groups, but zinc was significantly higher in the spleen (p<0.05) and lower in the heart (p<0.05) of WAR, compared to WBRs. Iron was significantly higher (p<0.05) in the heart and spleen of WBRs, compared to WARs. There were no extreme differences in the organ concentrations of trace elements between the two species, but, cumulatively, the WARs tend to have higher metallic concentrations in their system than the WBRs. The potential of these differences on the experimental results should not be overlooked and will serve as basis to further consider the complex interrelationships of these animals in their microenvironments and macroenvironments.     

Serum Levels of Zinc,Copper, Iron,Cobalt, Magnesium,and Selenium Elements in Children Diagnosed with <Emphasis Type="Italic">Giardia Intestinalis</Emphasis> and <Emphasis Type="Italic">Enterobiosis Vermicularis</Emphasis> in Hatay,Turkey

The intestinal parasites are noted to be an important health problem in Turkey as similarly reported in the globe. The aim of this study was to investigate the changes in total content of essential elements, namely, zinc, iron, copper, cobalt, magnesium, and selenium, in children infected with intestinal parasites aged between 6 and 12 years inhabiting in Hatay Province, Turkey. These essential elements were measured in the children/patient who was positive for intestinal parasites, Giardia intestinalis and Enterobius vermicularis. Scores were obtained from the positive study group (SG), and their age matched the healthy children control group (CG). Serological levels of zinc, iron, copper, cobalt, magnesium, and selenium were analyzed by Varian Liberty Series II inductively coupled plasma atomic emission spectrometer (ICP-AES). The mean magnesium concentrations were found to be statistically different at 95% confidence interval level between study groups. As a result of this study, selenium was found to be uncorrelated with all other elements examined; whereas, copper was observed to have statistically significant correlations with cobalt, magnesium, and zinc. In addition, cobalt-magnesium, cobalt-zinc, and magnesium-zinc metal pairs were found to have statistically significant correlations based on study findings.