共查询到20条相似文献,搜索用时 31 毫秒
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Kumar R Mustafiz A Sahoo KK Sharma V Samanta S Sopory SK Pareek A Singla-Pareek SL 《Plant molecular biology》2012,79(6):555-568
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Sumita Kumari Prabhjeet Singh Sneh L. Singla-Pareek Ashwani Pareek 《Molecular biotechnology》2009,42(2):195-204
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Mi-Jeong Jeong Seong-Kon Lee Beom-Gi Kim Tack-Ryoun Kwon Woo-Suk Cho Young-Taik Park Jin-Ohk Lee Hawk-Bin Kwon Myong-Ok Byun Soo-Chul Park 《Plant Cell, Tissue and Organ Culture》2006,85(2):151-160
We have isolated and characterized a putative rice MAPK gene (designated OsMAPK44) encoding for a protein of 593 amino acids that has the MAPK family signature and phosphorylation activation motif, TDY. Alignment of the predicted amino acid sequences of OsMAPK44 showed high homology with other rice MAPKs. Under normal conditions, the OsMAPK44 gene is highly expressed in root tissues, but relatively less in leaf and stem tissues of the japonica type rice plant (O. sativa L. Donggin). mRNA expression of the gene is highly inducible by salt and drought treatment, but not by cold treatment. Moreover, the mRNA level of the OsMAPK44 is up-regulated by exogenously applied Abscisic acid (ABA) and H2O2. When we compared the OsMAPK44 gene expression level between a salt sensitive indica cultivar (IR64) and a salt resistant indica cultivar (Pokkali), they showed some difference in expression kinetics with the salt treatment. OsMAPK44 gene expression in Pokkali was slightly up-regulated within 30 min and then disappeared rapidly, while IR64 maintained its expression for 1 h following down-regulation. Under the salinity stress, OsMAPK44 overexpression transgenic rice plants showed less damage and greater ratio of potassium and sodium than OsMAPK44 suppressed transgenic lines did, suggesting that OsMAPK44 may have a role to prevent damages due to working for favorable ion balance in the presence of salinity. 相似文献
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Mahmuda Binte Monsur Nasrin Akter Ivy M. Moynul Haque Mirza Hasanuzzaman Ayman EL Sabagh Md. Motiar Rohman 《Phyton》2020,89(3):497-517
The research was conducted to investigate comparative oxidative
damage including probable protective roles of antioxidant and glyoxalase systems
in rice (Oryza sativa L.) seedlings under salinity stress. Seedlings of two rice genotypes: Pokkali (tolerant) and BRRI dhan28 (sensitive) were subjected to 8 dSm−1
salinity stress for seven days in a hydroponic system. We observed significant variation between Pokkali and BRRI dhan28 in phenotypic, biochemical and molecular level under salinity stress. Carotenoid content, ion homeostasis, antioxidant
enzymes, ascorbate and glutathione redox system and proline accumulation may
help Pokkali to develop defense system during salinity stress. However, the activity antioxidant enzymes particularly superoxide dismutase (SOD), catalase (CAT)
and non-chloroplastic peroxidase (POD) were observed significantly higher in
Pokkali compared to salt-sensitive BRRI dhan28. Higher glyoxalase (Gly-I)
and glyoxalase (Gly-II) activity might have also accompanied Pokkali genotype
to reduce potential cytotoxic MG through non-toxic hydroxy acids conversion.
However, the efficient antioxidants and glyoxalase system together increased
adaptability in Pokkali during salinity stress. 相似文献
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Gene expression profiles during the initial phase of salt stress in rice 总被引:38,自引:0,他引:38
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Kawasaki S Borchert C Deyholos M Wang H Brazille S Kawai K Galbraith D Bohnert HJ 《The Plant cell》2001,13(4):889-906
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《Genomics》2020,112(5):3537-3548
DNA methylation governs gene regulation in plants in response to environmental conditions. Here, we analyzed role of DNA methylation under desiccation and salinity stresses in three (IR64, stress-sensitive; Nagina 22, drought-tolerant and Pokkali, salinity-tolerant) rice cultivars via bisulphite sequencing. Methylation in CG context within gene body and methylation in CHH context in distal promoter regions were positively correlated with gene expression. Hypomethylation in Nagina 22 and hypermethylation in Pokkali in response to desiccation and salinity stresses, respectively, were correlated with higher expression of few abiotic stress response related genes. Most of the differentially methylated and differentially expressed genes (DMR-DEGs) were cultivar-specific, suggesting an important role of DNA methylation in abiotic stress responses in rice in cultivar-specific manner. DMR-DEGs harboring differentially methylated cytosines due to DNA polymorphisms between the sensitive and tolerant cultivars in their promoter regions and/or coding regions were identified, suggesting the role of epialleles in abiotic stress responses. 相似文献
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Pannaga Krishnamurthy Kosala Ranathunge Rochus Franke H. S. Prakash Lukas Schreiber M. K. Mathew 《Planta》2009,230(1):119-134
Increasing soil salinity reduces crop yields worldwide, with rice being particularly affected. We have examined the correlation
between apoplastic barrier formation in roots, Na+ uptake into shoots and plant survival for three rice (Oryza sativa L.) cultivars of varying salt sensitivity: the salt-tolerant Pokkali, moderately tolerant Jaya and sensitive IR20. Rice plants
grown hydroponically or in soil for 1 month were subjected to both severe and moderate salinity stress. Apoplastic barriers
in roots were visualized using fluorescence microscopy and their chemical composition determined by gas chromatography and
mass spectrometry. Na+ content was estimated by flame photometry. Suberization of apoplastic barriers in roots of Pokkali was the most extensive
of the three cultivars, while Na+ accumulation in the shoots was the least. Saline stress induced the strengthening of these barriers in both sensitive and
tolerant cultivars, with increase in mRNAs encoding suberin biosynthetic enzymes being detectable within 30 min of stress.
Enhanced barriers were detected after several days of moderate stress. Overall, more extensive apoplastic barriers in roots
correlated with reduced Na+ uptake and enhanced survival when challenged with high salinity.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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Cattarin Theerawitaya Rujira Tisarum Thapanee Samphumphuang Taruhiro Takabe Suriyan Cha-um 《Physiology and Molecular Biology of Plants》2020,26(3):513-523
Salt affected soil inhibits plant growth, development and productivity, especially in case of rice crop. Ion homeostasis is a candidate defense mechanism in the salt tolerant plants or halophyte species, where the salt toxic ions are stored in the vacuoles. The aim of this investigation was to determine the OsNHX1 (a vacuolar Na+/H+ exchanger) and OsHKT2;1 (Na+/K+ transporter) regulation by salt stress (200 mM NaCl) in two rice cultivars, i.e. Pokkali (salt tolerant) and IR29 (salt susceptible), the accumulation of Na+ in the root and leaf tissues using CoroNa Green® staining dye and the associated physiological changes in test plants. Na+ content was largely increased in the root tissues of rice seedlings cv. Pokkali (15 min after salt stress) due to the higher expression of OsHKT2;1 gene (by 2.5 folds) in the root tissues. The expression of OsNHX1 gene in the leaf tissues was evidently increased in salt stressed seedlings of Pokkali, whereas it was unchanged in salt stressed seedlings of IR29. Na+ in the root tissues of both Pokkali and IR29 was enriched, when subjected to 200 mM NaCl for 12 h and easily detected in the leaf tissues of salt stressed plants exposed for 24 h, especially in cv. Pokkali. Moreover, the overexpression of OsNHX1 gene regulated the translocation of Na+ from root to leaf tissues, and compartmentation of Na+ into vacuoles, thereby maintaining the photosynthetic abilities in cv. Pokkali. Overall growth performance, maximum quantum yield (Fv/Fm), photon yield of PSII (ΦPSII) and net photosynthetic rate (Pn) was improved in salt stressed leaves of Pokkali than those in salt stressed IR29. 相似文献
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Transcript abundance profiles reveal larger and more complex responses of grapevine to chilling compared to osmotic and salinity stress 总被引:1,自引:0,他引:1
Tattersall EA Grimplet J DeLuc L Wheatley MD Vincent D Osborne C Ergül A Lomen E Blank RR Schlauch KA Cushman JC Cramer GR 《Functional & integrative genomics》2007,7(4):317-333
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Protective role of exogenous polyamines on salinity-stressed rice (Oryza sativa) plants 总被引:9,自引:0,他引:9
Chattopadhayay MK Tiwari BS Chattopadhyay G Bose A Sengupta DN Ghosh B 《Physiologia plantarum》2002,116(2):192-199
Salt-tolerant Pokkali rice plants accumulate higher polyamines (PAs) such as spermidine (Spd) and spermine (Spm) in response to salinity stress, while the sensitive cultivar M -1–48 is unable to maintain high titres of these PAs under similar conditions. The effects of the triamine Spd and the tetramine Spm on physiological and biochemical changes in 12-day-old rice seedlings were investigated during salinity stress to determine whether they could protect the sensitive plants from stress effects. At physiological concentrations Spd and Spm significantly prevented the leakage of electrolytes and amino acids from roots and shoots induced by salinity stress. To different degrees they also prevented chlorophyll loss, inhibition of photochemical reactions of photosynthesis as well as downregulation of chloroplast-encoded genes like psbA , psbB , psbE and rbcL , indicating a positive correlation between salt tolerance and accumulation of higher PAs in rice. The inhibitory effect of salinity stress and its reversal by exogenous PAs were more pronounced in the salt-sensitive M -1–48 plants than in the tolerant Pokkali plants. 相似文献
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Does exogenous glycinebetaine affect antioxidative system of rice seedlings under NaCl treatment? 总被引:8,自引:0,他引:8
The effect of exogenously applied glycinebetaine (GB) on the alleviation of damaging effects of NaCl treatment was studied in view of relative water content (RWC), malondialdehyde content, and the activity of some antioxidant enzymes in two rice (Oryza sativa L.) cultivars differing in salt tolerance (salt-tolerant Pokkali and--sensitive IR-28), comparatively. Both cultivars took up exogenously applied GB through their roots and accumulated it to considerable levels. Leaf RWC of both cultivars under salt treatment showed an increase with GB application. The activities of superoxide dismutase (SOD), ascorbate peroxidase (AP), catalase (CAT), and glutathione reductase (GR) increased in leaves of Pokkali, but peroxidase (POX) activity decreased under salinity. In IR-28, the activities of SOD, AP and POX increased, whereas CAT and GR decreased upon exposure to salt treatment. When compared to the salt-treated group alone, GB application decreased the activities of SOD, AP, CAT, and GR in Pokkali, whereas it increased the activities of CAT and AP in IR-28 under salinity. However, the activity of POX in IR-28 under salinity showed a decrease with GB application compared to the NaCl group. In addition, lipid peroxidation levels of both cvs. under salt treatment showed a decrease with GB treatment. Therefore, we conclude that GB protects both rice seedlings from salinity-induced oxidative stress. 相似文献
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Deposition pattern of hydrogen peroxide (H2O2) under salt stress (100 mM NaCl) was examined cytochemically in rice (Oryza sativa L. cv. Pokkali) through the reaction of H2O2 with cerium chloride (CeCl3) to produce electron dense precipitates of cerium perhydroxide. The distribution pattern of cerium perhydroxide precipitates
in leaf sheath was considerably different from other parts of rice under salinity stress. Cerium perhydroxide precipitates
were mainly accumulated on the tonoplast of leaf sheath under salinity, although they were localized on the cell wall and
plasma membrane in all other tissues such as leaf blade and root. 相似文献