共查询到20条相似文献,搜索用时 31 毫秒
1.
Brown Robert H.; Mitzner Wayne; Bulut Yonca; Wagner Elizabeth M. 《Journal of applied physiology》1997,82(2):491-499
Brown, Robert H., Wayne Mitzner, Yonca Bulut, and ElizabethM. Wagner. Effect of lung inflation in vivo on airways with smoothmuscle tone or edema. J. Appl.Physiol. 82(2): 491-499, 1997.Fibrousattachments to the airway wall and a subpleural surrounding pressurecan create an external load against which airway smooth muscle mustcontract. A decrease in this load has been proposed as a possible causeof increased airway narrowing in asthmatic individuals. To study theinteraction between the airways and the surrounding lung parenchyma, weinvestigated the effect of lung inflation on relaxed airways, airwayscontracted with methacholine, and airways made edematous by infusion ofbradykinin into the bronchial artery. Measurements were made inanesthetized sheep by using high-resolution computed tomography tovisualize changes in individual airways. During methacholine infusion,airway area was decreased but increased minimally with increases intranspulmonary pressure. Bradykinin infusion caused a 50% increase inairway wall area and a small decrease in airway luminal area. Incontrast to airways contracted with methacholine, the luminal areaafter bradykinin increased substantially with increases intranspulmonary pressure, reaching 99% of the relaxed area at totallung capacity. Thus airway edema by itself did not prevent fulldistension of the airway at lung volumes approaching total lungcapacity. Therefore, we speculate that if a deep inspiration fails torelieve airway narrowing in vivo, this must be a manifestation ofairway smooth muscle contraction and not airway wall edema. 相似文献
2.
Lung parenchymal shear modulus, airway wall remodeling, and bronchial hyperresponsiveness 总被引:1,自引:0,他引:1
Lambert, Rodney K., and Peter D. Paré. Lungparenchymal shear modulus, airway wall remodeling, and bronchialhyperresponsiveness. J. Appl. Physiol.83(1): 140-147, 1997.When airways narrow, either through theaction of smooth muscle shortening or during forced expiration, thelung parenchyma is locally distorted and provides an increasedperibronchial stress that resists the narrowing. Although thisinterdependence has been well studied, the quantitative significance ofairway remodeling to interdependence has not been elucidated. We haveused an improved computational model of the bronchial response tosmooth muscle agonists to investigate the relationships between airwaynarrowing (as indicated by airway resistance), parenchymal shearmodulus, adventitial thickening, and inner wall thickening at lungrecoil pressures of 4, 5, and 8 cmH2O. We have found that, at lowrecoil pressures, decreases in parenchymal shear modulus have asignificant effect that is comparable to that of moderate thickening ofthe airway wall. At higher lung recoil pressures, the effect isnegligible. 相似文献
3.
Sasaki, F., Y. Saitoh, L. Verburgt, and M. Okazawa.Airway wall dimensions during carbachol-inducedbronchoconstriction in rabbits. J. Appl.Physiol. 81(4): 1578-1583, 1996.Airway wall areais an important determinant of airway narrowing. We hypothesized thatin cross-sectioned peripheral airways, the wall area internal to theouter smooth muscle border (inner wall area) would decrease and theairway wall area external to the outer smooth the muscle layer(adventitial area) would increase during bronchoconstriction because ofthe relocation of blood and/or fluid between these compartments. To test this hypothesis, we used anesthetized open-chest rabbits and measured airway wall dimensions and smooth muscle shortening of membranous airways after carbachol-inducedbronchoconstriction using morphometric techniques. Acute (3-min) andsustained (40-min) bronchoconstriction was induced by aerosolnebulization of carbachol and compared with saline treatment. Afterphysiological measurements, the heart base was snared, and the lung andheart were excised en bloc and frozen by using liquid nitrogen while atranspulmonary pressure of 2 cmH2Owas maintained. The lung was processed for light-microscopicexamination by using a freeze substitution technique. Results show thatadventitial area was significantly decreased aftersustained but not acute bronchoconstriction. The mechanism of thischange, which contradicts our hypothesis, is unclear. However, thedecrease of adventitial area could increase rather than decrease theeffect of lung parenchymal tethering and attenuate airwaynarrowing. 相似文献
4.
Brown Robert H.; Mitzner Wayne; Wagner Elizabeth M. 《Journal of applied physiology》1997,83(2):366-370
Brown, Robert H., Wayne Mitzner, and Elizabeth M. Wagner.Interaction between airway edema and lung inflation onresponsiveness of individual airways in vivo. J. Appl.Physiol. 83(2): 366-370, 1997.Inflammatorychanges and airway wall thickening are suggested to cause increasedairway responsiveness in patients with asthma. In fivesheep, the dose-response relationships of individual airways weremeasured at different lung volumes to methacholine (MCh) before andafter wall thickening caused by the inflammatory mediator bradykininvia the bronchial artery. At 4 cmH2O transpulmonary pressure(Ptp), 5 µg/ml MCh constricted the airways to a maximum of 18 ± 3%. At 30 cmH2O Ptp, MCh resultedin less constriction (to 31 ± 5%). Bradykinin increased airwaywall area at 4 and 30 cmH2O Ptp(159 ± 6 and 152 ± 4%, respectively;P < 0.0001). At 4 cmH2O Ptp, bradykinin decreasedairway luminal area (13 ± 2%; P < 0.01), and the dose-response curve was significantly lower (P = 0.02). At 30 cmH2O, postbradykinin, the maximalairway narrowing was not significantly different (26 ± 5%;P = 0.76). Bradykinin produced substantial airway wall thickening and slight potentiation ofthe MCh-induced airway constriction at low lung volume. At high lung volume, bradykinin increased wall thickness but had no effecton the MCh-induced airway constriction. We conclude that inflammatoryfluid leakage in the airways cannot be a primary cause of airwayhyperresponsiveness. 相似文献
5.
Esophageal stimulation by hydrochloric acid causes neurogenic inflammation in the airways in guinea pigs 总被引:9,自引:0,他引:9
Hamamoto Junji; Kohrogi Hirotsugu; Kawano Osamu; Iwagoe Hajime; Fujii Kazuhiko; Hirata Nahomi; Ando Masayuki 《Journal of applied physiology》1997,82(3):738-745
Hamamoto, Junji, Hirotsugu Kohrogi, Osamu Kawano,Hajime Iwagoe, Kazuhiko Fujii, Nahomi Hirata, and Masayuki Ando.Esophageal stimulation by hydrochloric acid causes neurogenicinflammation in the airways in guinea pigs. J. Appl.Physiol. 82(3): 738-745, 1997.Toinvestigate whether tachykinins are released in the airways in responseto stimulation of the esophagus, we studied the airway plasmaextravasation induced by intraesophageal HCl in the presence or absenceof neutral endopeptidase inhibitor phosphoramidon and NK1-receptor antagonist FK-888 inanesthetized guinea pigs. The airway plasma leakage wasevaluated by measuring extravasated Evans blue dye in the animalspretreated with propranolol and atropine. Infusion of 1 N HCl into theesophagus significantly increased plasma extravasation in the trachea.Phosphoramidon significantly potentiated plasma extravasation in thetrachea and main bronchi, whereas FK-888 significantly inhibited that extravasation in a dose-related manner. In the capsaicin-treated animals, airway plasma extravasation was completely inhibited even inthe presence of phosphoramidon. Tracheal plasma extravasation potentiated by phosphoramidon was significantly inhibited in the bilateral vagotomized animals. These results suggest that1) tachykinin-like substances arereleased to cause plasma extravasation in the airways as a result ofintraesophageal HCl stimulation and2) there are neural pathwayscommunicating between the esophagus and airways, including the vagusnerve. 相似文献
6.
Matheson Melissa; Rynell Ann-Christine; McClean Melissa; Berend Norbert 《Journal of applied physiology》1998,84(1):77-81
Matheson, Melissa, Ann-Christine Rynell, Melissa McClean,and Norbert Berend. Relationship between airway microvascular leakage, edema, and baseline airway functions. J. Appl. Physiol. 84(1): 77-81, 1998.This study wasdesigned to examine the relationship among microvascular leakage,edema, and baseline airway function. Microvascular leakage was inducedin the airways of anesthetized, tracheostomized New Zealand Whiterabbits (n = 22) by using nebulized N-formyl-methionyl-leucyl-phenylalanine(10 mg) and was measured in the trachea by using the Evans blue dyetechnique. Airway wall thickness was assessed morphometrically in theright main bronchus after Formalin fixation at a pressure of 25 cmH2O. Areas calculated includedthe mucosal wall area, the adventitial wall area, the total wall area,and the percentage of total wall area consisting of blood vessels. Aneutrophil count was also performed by analyzing numbers of cells inboth the mucosal wall area and the adventitial wall area. Airwayfunction was assessed before and 30 min after challenge withN-formyl-methionyl-leucyl-phenylalanineby determining airway resistance, functional residual capacity,specific airway resistance, and flow-volume and pressure-volume curves(after paralysis of the animals with suxamethonium). The concentration of Evans blue dye in tracheal tissue ranged from 31.3 to 131.2 µg.There was a significant correlation between this concentration and boththe adventitial wall area (P < 0.01)and mucosal neutrophil numbers (P < 0.005). There was no correlation between Evans blue concentration andeither blood vessel area or changes in respiratory physiologyparameters before and after challenge. There was no significantdifference between any respiratory physiology measurements before andafter challenge. We conclude that an increase in microvascular leakagecorrelates with airway edema in the adventitia; however, these airwaychanges have no significant effect on airway elastic or resistiveproperties. 相似文献
7.
Shen, X., M. F. Wu, R. S. Tepper, and S. J. Gunst. Pharmacological modulation of the mechanicalresponse of airway smooth muscle to length oscillation.J. Appl. Physiol. 83(3): 739-745, 1997.Stretch and retraction of the airways caused by changes in lungvolume may play an important role in regulating airway reactivity. Westudied the effects of different pharmacological stimuli on airwaysmooth muscle to determine whether the muscle behavior during lengthoscillation can be modulated pharmacologically and to evaluate the roleof different activation mechanisms in determining its behavior duringthe oscillation. Active force decreased below the static isometricforce during the shortening phase of length oscillation, resulting inan overall depression of force during the length oscillation cycle.This pattern of response was unaffected by the contractile stimulus orlevel of activation, suggesting that it was caused by a mechanism that is independent of the level of activation of cross bridges. The normalized area of the length-force hysteresis loop (hysteresivity) differed depending on the stimulus used for contraction. Effects ofdifferent stimuli on hysteresivity were not correlated with theireffects on isotonic shortening velocity or isometric force, suggestingthat the pharmacological modulation of the behavior of airway smoothmuscle during length oscillation at these amplitudes cannot beaccounted for by the effects on the cross-bridge cycling rate. 相似文献
8.
Wiggs Barry R.; Hrousis Constantine A.; Drazen Jeffrey M.; Kamm Roger D. 《Journal of applied physiology》1997,83(6):1814-1821
Wiggs, Barry R., Constantine A. Hrousis, Jeffrey M. Drazen,and Roger D. Kamm. On the mechanism of mucosalfolding in normal and asthmatic airways. J. Appl.Physiol. 83(6): 1814-1821, 1997.Previous studies have demonstrated that the airwaywall in asthma and chronic obstructive pulmonary disease is markedly thickened. It has also been observed that when the smooth muscle constricts the mucosa buckles, forming folds that penetrate into theairway lumen. This folding pattern may influence the amount of luminalobstruction associated with smooth muscle activation. A finite-elementanalysis of a two-layer composite model for an airway is used toinvestigate the factors that determine the mucosal folding pattern andhow it is altered as a result of changes in the thickness or stiffnessof the different layers that comprise the airway wall. Resultsdemonstrate that the most critical physical characteristic is thethickness of the thin inner layer of the model. Thickening of thisinner layer likely is represented by the enhanced subepithelialcollagen deposition seen in asthma. Other findings show a high shearstress at or near the epithelial layer, which may explain thepronounced epithelial sloughing that occurs in asthma, and steepgradients in pressure that could cause significant shifts of liquidbetween wall compartments or between the wall and luminal or vascularspaces. 相似文献
9.
Effect of stochastic heterogeneity on lung impedance during acute bronchoconstriction: a model analysis 总被引:2,自引:0,他引:2
Thorpe, C. William, and Jason H. T. Bates. Effect ofstochastic heterogeneity on lung impedance during acutebronchoconstriction: a model analysis. J. Appl.Physiol. 82(5): 1616-1625, 1997.In a previousstudy (J. H. T. Bates, A. M. Lauzon, G. S. Dechman, G. N. Maksym, and T. F. Schuessler. J. Appl.Physiol. 76: 616-626, 1994), we investigated theacute changes in isovolume lung mechanics immediately after a bolusinjection of histamine. We found that dynamic resistance and elastanceincreased progressively in the 80-s period after injection, whereas theestimated tissue hysteresivity reached a stable plateau after ~25 s.In the present study, we developed a computer model of the lung toinvestigate the mechanisms responsible for these observations. Themodel conforms to Horsfield's morphometry, with the addition ofcompliant airways and structural damping tissue units. Using thismodel, we simulated the time course of acute bronchoconstriction afterintravenous administration of a bolus of bronchial agonist.Heterogeneity was induced by randomly varying the values of the maximalairway smooth muscle contraction and the tissue response to theagonist. Our results demonstrate that much of the increase in lungimpedance observed in our previous study can be produced purely by theeffects of airway heterogeneity. However, we were only able toreproduce the plateauing of hysteresivity by assigning a minimum radius to each airway, beyond which it would immediately snap completely shut.We propose that airway closure played an important role in ourexperimental observations. 相似文献
10.
Grossmann Gertie; Suzuki Yasuhiro; Robertson Bengt; Kobayashi Tsutomu; Berggren Per; Li Wen-Zhi; Song Guo-Wei; Sun Bo 《Journal of applied physiology》1997,82(6):2003-2010
Grossmann, Gertie, Yasuhiro Suzuki, Bengt Robertson, TsutomuKobayashi, Per Berggren, Wen-Zhi Li, Guo-Wei Song, and Bo Sun.Pathophysiology of neonatal lung injury induced by monoclonal antibody to surfactant protein B. J. Appl.Physiol. 82(6): 2003-2010, 1997.Near-termnewborn rabbits were exposed via the airways to a monoclonal antibodyto surfactant protein B and ventilated for 0-120 min. Controlanimals received nonspecific rabbit or mouse immunoglobulin G, saline,or no material via the airways. Administration of the antibody at 40mg/kg elicited an immediate, significant fall in lung-thorax complianceassociated with progressive intra-alveolar edema and/oralveolar collapse and necrosis and desquamation of airway epithelium,and hyaline membranes. The vascular-to-alveolar leak of human albuminand human immunoglobulin G, injected intravenously at birth anddetermined in lung lavage fluid 60-120 min after instillation ofthe antibody, was 1.8% for the left lung, with no difference betweenthe markers. The average leak in control animals ventilated for 120 minwas <0.3% (P < 0.05). Cytospin preparations of lung lavage fluid from animals exposed to the antibodyshowed significantly increased recruitment of neutrophilic granulocytes. The pathology and pathophysiology of neonatal lung injuryinduced by the monoclonal antibody to surfactant protein B probablyreflect a combination of direct inactivation of surfactant and aninflammatory response triggered by the immune reaction. 相似文献
11.
Pedersen O. F.; Brackel H. J.L.; Bogaard J. M.; Kerrebijn K. F. 《Journal of applied physiology》1997,83(5):1721-1732
Pedersen, O. F., H. J. L. Brackel, J. M. Bogaard, and K. F. Kerrebijn. Wave-speed-determined flow limitation at peak flow innormal and asthmatic subjects. J. Appl.Physiol. 83(5): 1721-1732, 1997.The purpose ofthis study was to examine whether peak expiratory flow is determined bythe wave-speed flow-limiting mechanism. We examined 17 healthy subjectsand 11 subjects with stable asthma, the latter treated with inhaledbronchodilators and corticosteroids. We used an esophageal balloon anda Pitot-static probe positioned at five locations between the rightlower lobe and midtrachea to obtain dynamic area-transmural pressure(A-Ptm) curves as described (O. F. Pedersen, B. Thiessen, and S. Lyager. J. Appl.Physiol. 52: 357-369, 1982). From these curves weobtained cross-sectional area (A)and airway compliance (Caw = dA/dPtm) at PEF, calculated flow at wave speed {ws = A[A/(Caw*)0.5],where is density} and speed index is (SI = /ws). In 13 of 15 healthy andin 4 of 10 asthmatic subjects, who could produce satisfactory curves,SI at PEF was >0.9 at one or more measured positions. Alveolarpressure continued to increase after PEF was achieved, suggesting flowlimitation somewhere in the airway in all of these subjects. Weconclude that wave speed is reached in central airways at PEF in mostsubjects, but it cannot be excluded that wave speed is also reached inmore peripheral airways. 相似文献
12.
Freed Arthur N.; Taskar Varsha; Schofield Brian; Omori Chiharu 《Journal of applied physiology》1996,81(6):2461-2467
Freed, Arthur N., Varsha Taskar, Brian Schofield, andChiharu Omori. Effect of furosemide on hyperpnea-induced airway obstruction, injury, and microvascular leakage. J. Appl. Physiol. 81(6): 2461-2467, 1996.Furosemideattenuates hyperpnea-induced airway obstruction (HIAO) in asthmaticsubjects via unknown mechanism(s). We studied the effect of furosemideon dry air-induced bronchoconstriction, mucosal injury, andbronchovascular hyperpermeability in a canine model of exercise-inducedasthma. Peripheral airway resistance (Rp) was recorded before and aftera 2-min dry-air challenge (DAC) at 2,000 ml/min. After pretreatmentwith aerosolized saline containing 0.75% dimethyl sulfoxide, DACincreased Rp 72 ± 11% (SE, n = 7) above baseline; aerosolized furosemide(103 M) reduced thisresponse by ~50 ± 6% (P < 0.01). To assess bronchovascular permeability, colloidal carbon wasinjected (1 ml/kg iv) 1 min before DAC, and after 1 h, the vehicle- andfurosemide-treated airways were prepared for morphometric analysis.Light microscopy confirmed previous studies showing that DAC damagedthe airway epithelium and enhanced bronchovascular permeability.Furosemide did not inhibit dry air-induced mucosal injury orbronchovascular hyperpermeability and in fact tended to increase airwaydamage and vascular leakage. This positive trend toward enhancedbronchovascular permeability in DAC canine peripheral airways isconsistent with the hypothesis that furosemide inhibits HIAO in part byenhancing microvascular leakage and thus counterbalancing theevaporative water loss that occurs during hyperpnea. 相似文献
13.
Relationship between heterogeneous changes in airway morphometry and lung resistance and elastance 总被引:3,自引:0,他引:3
Lutchen, Kenneth R., and Heather Gillis. Relationshipbetween heterogeneous changes in airway morphometry and lung resistanceand elastance. J. Appl. Physiol.83(4): 1192-1201, 1997.We present a dog lung model to predictthe relation between inhomogeneous changes in airway morphometry andlung resistance (RL) andelastance (EL) for frequenciessurrounding typical breathing rates. TheRL andEL were sensitive in distinctways to two forms of peripheral constriction. First, when there is alarge and homogeneous constriction, theRL increases uniformly over thefrequency range. The EL israther unaffected below 1 Hz but then increases with frequencies up to5 Hz. This increase is caused by central airway wallshunting. Second, the RL andEL are extremely sensitive to mild inhomogeneous constriction in which a few highly constricted ornearly closed airways occur randomly throughout theperiphery. This results in extreme increases in the levelsand frequency dependence of RLand EL but predominantly attypical breathing rates (<1 Hz). Conversely, theRL andEL are insensitive to highly inhomogeneous airway constriction that does not produce any nearly closed airways. Similarly, alterations in theRL andEL due to central airway wallshunting are not likely until the preponderance of the peripheryconstricts substantially. The RLand EL spectra are far moresensitive to these two forms of peripheral constriction than toconstriction conditions known to occur in the central airways. On thebasis of these simulations, we derived a set of qualitative criteria toinfer airway constriction conditions from RL andEL spectra. 相似文献
14.
Adler Andy; Cowley Elizabeth A.; Bates Jason H.T.; Eidelman David H. 《Journal of applied physiology》1998,85(1):231-237
The constrictionof pulmonary airways is limited by the tethering effect exerted byparenchymal attachments. To characterize this tethering effect at thescale of intraparenchymal airways, we studied the pattern ofparenchymal distortion due to bronchoconstriction in a rat lung explantsystem. First, we measured the elastic modulus under tension for 2%(wt/vol) agarose alone (37.6 ± 1.5 kPa) and for agarose-filled lung(5.7 ± 1.3 kPa). The latter is similar to the elastic modulus ofair-filled lung at total lung capacity (4.5-6 kPa) (S. J. Lai-Fook, T. A. Wilson, R. E. Hyatt, and J. R. Rodarte.J. Appl. Physiol. 40: 508-513,1976), suggesting that explants can be used as a model of lung tissuedistortion. Subsequently, confocal microscopic images of fluorescentlylabeled 0.5-mm-thick explants prepared from agarose-filled rat lungsinflated to total lung capacity (48 ml/kg) were acquired. Images weretaken before and after airway constriction was induced by directapplication of 10 mM methacholine, and the pattern of parenchymaldistortion was measured from the displacement of tissue landmarksidentified in each image for 14 explants. The magnitude of the radialcomponent of tissue displacement was calculated as a function ofdistance from the airway wall and characterized by a parameter,b, describing the rate at which tissuemovement decreased with radial distance. The parameterb was 0.994 ± 0.19 (SE), which isclose to the prediction of b = 1 ofmicromechanical modeling (T. A. Wilson. J. Appl.Physiol. 33: 472-478, 1972). There was significantvariability in b, however, which wascorrelated with the fractional reduction in airway diameter (r = 0.496). Additionally, parenchymaldistortion showed significant torsion with respect to the radialdirection. This torsion was similar in concentric zones around theairway, suggesting that it originates from inhomogeneity in theparenchyma rather than inhomogeneous airway constriction. Our resultsdemonstrate the significance of the nonlinear mechanical properties ofalveolar walls and the anisotropy of the parenchyma in determining the nature of airway-parenchymal interdependence. 相似文献
15.
Wang C. G.; Almirall J. J.; Dolman C. S.; Dandurand R. J.; Eidelman D. H. 《Journal of applied physiology》1997,82(5):1445-1452
Wang, C. G., J. J. Almirall, C. S. Dolman, R. J. Dandurand,and D. H. Eidelman. In vitro bronchial responsiveness in twohighly inbred rat strains. J. Appl.Physiol. 82(5): 1445-1452, 1997.We investigatedmethacholine (MCh)-induced bronchoconstriction in explanted airwaysfrom Fischer and Lewis rats. Lung explants, 0.5- to 1.0-mm thick, wereprepared from agarose-inflated lungs of anesthetized 8- to 12-wk-oldmale rats. After overnight culture, videomicroscopy was used to recordbaseline images of the individual airways. Dose-response curves to MChwere then constructed by repeated administration of MCh; airways werereimaged 10 min after each MCh administration. Airway internal luminalarea(Ai)was measured at successive MCh concentrations from109 to101 M. Inaddition to the effective concentration leading to 50% of the achievedmaximal response, we also determined the effective concentrationleading to a 40% reduction inAi.Both the effective concentration leading to 50% of the achievedmaximal response and the concentration leading to a 40% reduction inAiwere significantly lower among Fischer rat airways(P < 0.05). Airway closure was morecommon among Fischer rat airways (17%) than among those of Lewis rats(7.5%). Responsiveness of Fischer rat airways was more heterogeneousthan among Lewis airways; a larger number of Fischer rat airwaysexhibited high sensitivity to MCh. There was no relationship betweenresponsiveness and baselineAiin either strain. In a second experiment, we measured the rate ofcontraction of explanted airways from lungs inflated to 50, 75, and100% of total lung capacity. The average rate of contraction in thefirst 15 s was higher in Fischer rat airways at each inflation volume.These data indicate that the hyperresponsiveness of the Fischer rat reflects the responsiveness of individual airways throughout the airwaytree and are consistent with the notion that in this model hyperresponsiveness is an intrinsic property of airway smooth muscle. 相似文献
16.
Effects of edema on small airway narrowing 总被引:1,自引:0,他引:1
Wagner, Elizabeth M. Effects of edema on small airwaynarrowing. J. Appl. Physiol. 83(3):784-791, 1997.Numerous mediators of inflammation have beendemonstrated to cause airway microvascular fluid and proteinextravasation. That fluid extravasation results in airway wall edemaleading to airway narrowing and enhanced reactivity has not beenconfirmed. In anesthetized, ventilated sheep(n = 30), airway vascularfluid extravasation was induced by infusing bradykinin(106 M) through acannulated, blood-perfused bronchial artery. Airway wall edema andluminal narrowing were determined morphometrically. Airway reactivityto methacholine (MCh; 10 µg/ml, intrabronchial artery) was determinedby measuring conducting airway resistance (Raw) by forced oscillation.Raw measurements were made and lung lobes were excised and quick frozenbefore or after a 1-h bradykinin infusion. In 10 airways per lobe(range 0.2- to 2.0-mm relaxed diameter), wall area occupied 32 ± 2% (SE) of the total normalized airway area(n = 9). Bradykinin infusion increasedwall area to 42 ± 5% (P = 0.02);luminal area decreased by <5%; and smooth muscle perimeter, ameasure of smooth muscle constriction, was not altered(n = 5). Raw showed nochange from baseline (1.4 ± 0.4 cmH2O · l1 · s)after bradykinin infusion (n = 10).During MCh challenge, Raw increased by 3.2 ± 04 cmH2O · l1 · s,and this change did not differ after administration of bradykinin. MChchallenge caused similar decreases in smooth muscle perimeter (10%)and luminal area (72 vs. 68%) before and after bradykinin infusion.However, the time constant of recovery of Raw from MCh constriction wasincreased from control (40 ± 3 s) to 57 ± 10 s after bradykinininfusion (P = 0.03). When lung lobeswere excised at the same time after MCh challenge was terminated(n = 5), luminal area was greaterbefore bradykinin infusion than after (86 vs. 78%;P = 0.007), as was smooth muscleperimeter. The results of this study demonstrate that airway wall edemalimits relaxation after induced constriction rather than enhancingconstriction. 相似文献
17.
Bates Jason H.T.; Schuessler Thomas F.; Dolman Carrie; Eidelman David H. 《Journal of applied physiology》1997,82(1):55-62
Bates, Jason H. T., Thomas F. Schuessler, Carrie Dolman, andDavid H. Eidelman. Temporal dynamics of acute isovolume bronchoconstriction in the rat. J. Appl.Physiol. 82(1): 55-62, 1997.The time course oflung impedance changes after intravenous injection of bronchial agonisthave produced significant insights into the mechanisms ofbronchoconstriction in the dog (J. H. T. Bates, A.-M. Lauzon, G. S. Dechman, G. N. Maksym, and T. F. Shuessler. J. Appl.Physiol. 76: 616-626, 1994). We studied the timecourse of acute induced bronchoconstriction in five anesthetizedparalyzed open-chest rats injected intravenously with a bolus ofmethacholine. For the 16 s immediately after injection, we held thelung volume constant while applying small-amplitude flow oscillationsat 1.48, 5.45, and 19.69 Hz simultaneously, which provided us withcontinuous estimates of lung resistance(RL) and elastance(EL) at eachfrequency. This procedure was repeated at initial lung inflationpressures of 0.2, 0.4, and 0.6 kPa. BothRL andEL increased progressively aftermethacholine administration; however, the rate of change ofEL increased dramatically asfrequency was increased, whereas RL remained relativelyindependent of frequency. We interpret these findings in terms of athree-compartment model of the rat lung, featuring two parallelalveolar compartments feeding into a central airway compartment. Modelsimulations support the notions that both central airway shunting andregional ventilation inhomogeneity developed to a significant degree inour constricted rats. We also found that the rates of increase in bothRL andEL were greatly enhanced as theinitial lung inflation pressure was reduced, in accord with the notionthat parenchymal tethering is an important mechanism limiting theextent to which airways can narrow when their smooth muscle isstimulated to contract. 相似文献
18.
Chen, Zibin, and Frederic L. Eldridge. Inputs fromupper airway affect firing of respiratory-associated midbrain neurons. J. Appl. Physiol. 83(1): 196-203, 1997.In 16 decerebrated unanesthetized cats, we studied effects ofneural inputs from upper airway on firing of 62 mesencephalic neuronsthat also developed respiratory-associated (RA) rhythmic firing whenrespiratory drive was high [Z. Chen, F. L. Eldridge, and P.G.Wagner. J. Physiol. (Lond.) 437:305-325, 1991] and on firing of 16 neurons that did notdevelop the rhythmic firing (non-RA neurons). Activity in RA neuronsincreased after mechanical expansion of pharynx (45% of those tested)or larynx (68%) and after stimulation of glossopharyngeal (50%) orsuperior laryngeal nerves (77%). The increased neuronal firingoccurred despite decreases or abolition of respiratory activity(expressed in phrenic nerve). Neuronal firing also increased aftermechanical stimulation of nasal mucosa (66%) or by jetsof air directed into the nares (48%) and after lightbrushing of nasal skin (~40%). Most stimuli led to decreased firingin a smaller number of neurons, and some neurons showed no response.None of the non-RA neurons developed an increase of firing after any ofthe stimuli, although one had decreased firing after stimulation of thesuperior laryngeal nerve. We conclude that inputs from the upper airwayand nasal skin have independent modulatory effects on the samemesencephalic neurons that are stimulated by ascending rhythmic RAinput from the medulla. These findings may have relevance to generationof the sensation of dyspnea. 相似文献
19.
Shen X.; Bhargava V.; Wodicka G. R.; Doerschuk C. M.; Gunst S. J.; Tepper R. S. 《Journal of applied physiology》1996,81(6):2637-2643
Shen, X., V. Bhargava, G. R. Wodicka, C. M. Doerschuk, S. J. Gunst, and R. S. Tepper. Greater airway narrowing in immature thanin mature rabbits during methacholine challenge. J. Appl. Physiol. 81(6): 2637-2643, 1996.It hasbeen demonstrated that methacholine (MCh) challenge produces a greaterincrease in lung resistance in immature than in mature rabbits (R. S. Tepper, X. Shen, E. Bakan, and S. J. Gunst.J. Appl. Physiol. 79: 1190-1198, 1995). To determine whether this maturational difference in the response to MCh was primarily related to changes in airway resistance (Raw) or changes in tissue resistance, we assessed airway narrowing in1-, 2-, and 6-mo-old rabbits during intravenous MCh challenge (0.01-5.0 mg/kg). Airway narrowing was determined frommeasurements of Raw in vivo and from morphometric measurements on lungsections obtained after rapidly freezing the lung after the MChchallenge. The fold increase in Raw was significantly greater for 1- and 2-mo-old animals than for 6-mo-old animals. Similarly, the degree of airway narrowing assessed morphometrically was significantly greaterfor 1- and 2-mo-old animals than for 6-mo-old animals. The foldincrease in Raw was highly correlated with the degree of airwaynarrowing assessed morphometrically(r2 = 0.82, P < 0.001). We conclude that thematurational difference in the effect of MCh on lung resistance isprimarily caused by greater airway narrowing in the immature rabbits. 相似文献
20.
Selective stimulation of jugular ganglion afferent neurons in guinea pig airways by hypertonic saline 总被引:2,自引:0,他引:2
Pedersen Karen E.; Meeker Sonya N.; Riccio Margerita M.; Undem Bradley J. 《Journal of applied physiology》1998,84(2):499-506
Pedersen, Karen E., Sonya N. Meeker, Margerita M. Riccio,and Bradley J. Undem. Selective stimulation ofjugular ganglion afferent neurons in guinea pig airways by hypertonicsaline. J. Appl. Physiol. 84(2):499-506, 1998.We evaluated the ability of hyperosmolar stimulito activate afferent nerves in the guinea pig trachea and main bronchiand investigated the neural pathways involved. By usingelectrophysiological techniques, studies in vitro examined the effectof hyperosmolar solutions of sodium chloride (hypertonic saline) onguinea pig airway afferent nerve endings arising from either vagalnodose or jugular ganglia. The data reveal a differential sensitivityof airway afferent neurons to activation with hypertonic saline.Afferent fibers (both A and C fibers) with cell bodies located injugular ganglia were much more sensitive to stimulation with hypertonicsaline, compared with afferent neurons with cell bodies located innodose ganglia. Additional studies in vivo demonstrated that inhalationof aerosols of hypertonic saline induced plasma extravasation in guineapig trachea that was mediated via tachykininNK1 receptors. Identification of adifferential sensitivity of guinea pig airway afferent nerves tohypertonic saline leads to the speculation that airway responses tohyperosmolar stimuli may result from activation of afferent neuronsoriginating predominantly from the jugular ganglion. 相似文献